(Linear) (Six-base) MAPSORT of: KIEE5950.seq  Check: 2068  from: 1  to: 936



KIEE5950.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     864     936 
   Size:        864      72
 
AccI GT'mk_AC

Cuts at:      0     168     936 
   Size:        168     768
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     936 
   Size:        286     650
 
AflIII A'CryG_T

Cuts at:      0     320     936 
   Size:        320     616
 
ApaLI G'TGCA_C

Cuts at:      0     572     936 
   Size:        572     364
 
ApoI r'AATT_y

Cuts at:      0     459     936 
   Size:        459     477
 
BanII G_rGCy'C

Cuts at:      0     605     936 
   Size:        605     331
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     442     936 
   Size:        442     494
 
BclI T'GATC_A

Cuts at:      0     149     936 
   Size:        149     787
 
BglI GCCn_nnn'nGGC

Cuts at:      0     565     936 
   Size:        565     371
 
Bme1580I G_kGCm'C

Cuts at:      0     576     936 
   Size:        576     360
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     936 
   Size:        379     557
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     677     936 
   Size:        677     259
 
BsiHKAI G_wGCw'C

Cuts at:      0     548     576     936 
   Size:        548      28     360

  Fragments arranged by size: 

                 548     360      28
 
Bsp1286I G_dGCh'C

Cuts at:      0     548     576     605     936 
   Size:        548      28      29     331

  Fragments arranged by size: 

                 548     331      29      28
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     864     936 
   Size:        864      72
 
BsrGI T'GTAC_A

Cuts at:      0     924     936 
   Size:        924      12
 
BstYI r'GATC_y

Cuts at:      0     896     936 
   Size:        896      40
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     705     740     936 
   Size:        705      35     196

  Fragments arranged by size: 

                 705     196      35
 
EaeI y'GGCC_r

Cuts at:      0     194     557     795     936 
   Size:        194     363     238     141

  Fragments arranged by size: 

                 363     238     194     141
 
EarI CTCTTCn'nnn_

Cuts at:      0     654     936 
   Size:        654     282
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     379     936 
   Size:        286      93     557

  Fragments arranged by size: 

                 557     286      93
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     432     726     936 
   Size:        432     294     210

  Fragments arranged by size: 

                 432     294     210
 
MscI TGG'CCA

Cuts at:      0     196     797     936 
   Size:        196     601     139

  Fragments arranged by size: 

                 601     196     139
 
MslI CAynn'nnrTG

Cuts at:      0     737     936 
   Size:        737     199
 
NspI r_CATG'y

Cuts at:      0     324     936 
   Size:        324     612
 
PciI A'CATG_T

Cuts at:      0     320     936 
   Size:        320     616
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     511     936 
   Size:        511     425
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     152     184     936 
   Size:        152      32     752

  Fragments arranged by size: 

                 752     152      32
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     565     936 
   Size:        565     371
 
SmlI C'TyrA_G

Cuts at:      0     692     936 
   Size:        692     244
 
SspI AAT'ATT

Cuts at:      0     920     936 
   Size:        920      16
 
StuI AGG'CCT

Cuts at:      0     712     936 
   Size:        712     224
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      34     591     936 
   Size:         34     557     345

  Fragments arranged by size: 

                 557     345      34
 
TatI w'GTAC_w

Cuts at:      0     398     924     936 
   Size:        398     526      12

  Fragments arranged by size: 

                 526     398      12
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII    ApaLI     ApoI    BanII    BciVI
     BclI     BglI Bme1580I     BpmI    BpuEI  BsiHKAI Bsp1286I    BspMI
    BsrGI    BstYI    CspCI     EaeI     EarI  Eco57MI     MmeI     MscI
     MslI     NspI     PciI    PflMI     PsrI     SfiI     SmlI     SspI
     StuI    TaqII     TatI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StyI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI