(Linear) (Six-base) MAPSORT of: KIEE5945.seq  Check: 5691  from: 1  to: 936



KIEE5945.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     677     936 
   Size:         83     594     259

  Fragments arranged by size: 

                 594     259      83
 
AleI CACnn'nnGTG

Cuts at:      0      25     936 
   Size:         25     911
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     393     936 
   Size:        393     543
 
AlwNI CAG_nnn'CTG

Cuts at:      0      61     936 
   Size:         61     875
 
AseI AT'TA_AT

Cuts at:      0     683     936 
   Size:        683     253
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     306     927     936 
   Size:        306     621       9

  Fragments arranged by size: 

                 621     306       9
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     446     936 
   Size:        446     490
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     915     936 
   Size:        145     770      21

  Fragments arranged by size: 

                 770     145      21
 
Bpu10I CC'TnA_GC

Cuts at:      0     246     936 
   Size:        246     690
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      88     336     936 
   Size:         88     248     600

  Fragments arranged by size: 

                 600     248      88
 
BseYI C'CCAG_C

Cuts at:      0     120     936 
   Size:        120     816
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     549     936 
   Size:        549     387
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     729     936 
   Size:        729     207
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     621     936 
   Size:        621     315
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     469     936 
   Size:        469     467
 
EarI CTCTTCn'nnn_

Cuts at:      0      59     801     936 
   Size:         59     742     135

  Fragments arranged by size: 

                 742     135      59
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     139     936 
   Size:        139     797
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     145     677     915     936 
   Size:         83      62     532     238      21

  Fragments arranged by size: 

                 532     238      83      62      21
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     809     936 
   Size:        809     127
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     366     398     731     763     936 
   Size:        366      32     333      32     173

  Fragments arranged by size: 

                 366     333     173      32      32
 
HindIII A'AGCT_T

Cuts at:      0     707     936 
   Size:        707     229
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     290     936 
   Size:        290     646
 
MslI CAynn'nnrTG

Cuts at:      0      25      43     175     503     586     619     936 
   Size:         25      18     132     328      83      33     317

  Fragments arranged by size: 

                 328     317     132      83      33      25      18
 
PstI C_TGCA'G

Cuts at:      0     382     936 
   Size:        382     554
 
SfcI C'TryA_G

Cuts at:      0     378     936 
   Size:        378     558
 
StyI C'CwwG_G

Cuts at:      0     800     936 
   Size:        800     136
 
XmnI GAAnn'nnTTC

Cuts at:      0     709     936 
   Size:        709     227
 

 Enzymes that do cut:

     AcuI     AleI     AlfI    AlwNI     AseI     BbsI     BmrI     BpmI
   Bpu10I    BseRI    BseYI     BsgI    BsmBI    BstXI     DrdI     EarI
     EciI  Eco57MI     FalI    Hin4I  HindIII     MmeI     MslI     PstI
     SfcI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI     ApaI    ApaLI     ApoI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EagI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI