Restriction map for
pFN21AE5943
(Linear) (Six-base) MAPSORT of: KIEE5943.seq Check: 8450 from: 1 to: 936
KIEE5943.orf
With 182 enzymes: SgfI *
December 5, 2014 13:15 ..
AgeI A'CCGG_T
Cuts at: 0 362 936
Size: 362 574
AhdI GACnn_n'nnGTC
Cuts at: 0 769 936
Size: 769 167
AleI CACnn'nnGTG
Cuts at: 0 298 734 936
Size: 298 436 202
Fragments arranged by size:
436 298 202
AlwNI CAG_nnn'CTG
Cuts at: 0 514 936
Size: 514 422
ApaI G_GGCC'C
Cuts at: 0 900 936
Size: 900 36
ApoI r'AATT_y
Cuts at: 0 31 936
Size: 31 905
BamHI G'GATC_C
Cuts at: 0 638 936
Size: 638 298
BanI G'GyrC_C
Cuts at: 0 287 936
Size: 287 649
BanII G_rGCy'C
Cuts at: 0 900 936
Size: 900 36
BbvCI CC'TCA_GC
Cuts at: 0 913 936
Size: 913 23
BciVI GTATCCnnnnn_n'
Cuts at: 0 324 936
Size: 324 612
BglI GCCn_nnn'nGGC
Cuts at: 0 352 422 936
Size: 352 70 514
Fragments arranged by size:
514 352 70
Bme1580I G_kGCm'C
Cuts at: 0 900 936
Size: 900 36
Bpu10I CC'TnA_GC
Cuts at: 0 545 913 936
Size: 545 368 23
Fragments arranged by size:
545 368 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 936
Size: 538 398
BsaI GGTCTCn'nnnn_
Cuts at: 0 747 936
Size: 747 189
BsaWI w'CCGG_w
Cuts at: 0 362 936
Size: 362 574
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 568 657 901 936
Size: 28 540 89 244 35
Fragments arranged by size:
540 244 89 35 28
BseYI C'CCAG_C
Cuts at: 0 117 615 634 936
Size: 117 498 19 302
Fragments arranged by size:
498 302 117 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 936
Size: 373 563
Bsp1286I G_dGCh'C
Cuts at: 0 900 936
Size: 900 36
BspHI T'CATG_A
Cuts at: 0 404 936
Size: 404 532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 936
Size: 134 802
BsrFI r'CCGG_y
Cuts at: 0 362 540 936
Size: 362 178 396
Fragments arranged by size:
396 362 178
BstEII G'GTnAC_C
Cuts at: 0 840 936
Size: 840 96
BstXI CCAn_nnnn'nTGG
Cuts at: 0 105 936
Size: 105 831
BstYI r'GATC_y
Cuts at: 0 638 724 936
Size: 638 86 212
Fragments arranged by size:
638 212 86
Bsu36I CC'TnA_GG
Cuts at: 0 874 936
Size: 874 62
BtgI C'CryG_G
Cuts at: 0 605 836 936
Size: 605 231 100
Fragments arranged by size:
605 231 100
BtsI GCAGTG_nn'
Cuts at: 0 385 610 936
Size: 385 225 326
Fragments arranged by size:
385 326 225
EaeI y'GGCC_r
Cuts at: 0 140 344 371 602 936
Size: 140 204 27 231 334
Fragments arranged by size:
334 231 204 140 27
EarI CTCTTCn'nnn_
Cuts at: 0 484 649 936
Size: 484 165 287
Fragments arranged by size:
484 287 165
EcoNI CCTnn'n_nnAGG
Cuts at: 0 295 936
Size: 295 641
EcoO109I rG'GnC_Cy
Cuts at: 0 549 896 897 936
Size: 549 347 1 39
Fragments arranged by size:
549 347 39 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 716 748 755 787 936
Size: 716 32 7 32 149
Fragments arranged by size:
716 149 32 32 7
HincII GTy'rAC
Cuts at: 0 207 497 936
Size: 207 290 439
Fragments arranged by size:
439 290 207
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 936
Size: 215 721
MscI TGG'CCA
Cuts at: 0 373 604 936
Size: 373 231 332
Fragments arranged by size:
373 332 231
MslI CAynn'nnrTG
Cuts at: 0 172 298 734 847 936
Size: 172 126 436 113 89
Fragments arranged by size:
436 172 126 113 89
NcoI C'CATG_G
Cuts at: 0 836 936
Size: 836 100
PpuMI rG'GwC_Cy
Cuts at: 0 549 936
Size: 549 387
PshAI GACnn'nnGTC
Cuts at: 0 929 936
Size: 929 7
PspOMI G'GGCC_C
Cuts at: 0 896 936
Size: 896 40
PstI C_TGCA'G
Cuts at: 0 71 936
Size: 71 865
SexAI A'CCwGG_T
Cuts at: 0 104 936
Size: 104 832
SfcI C'TryA_G
Cuts at: 0 67 629 867 936
Size: 67 562 238 69
Fragments arranged by size:
562 238 69 67
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 352 936
Size: 352 584
SmlI C'TyrA_G
Cuts at: 0 553 936
Size: 553 383
StyI C'CwwG_G
Cuts at: 0 692 836 936
Size: 692 144 100
Fragments arranged by size:
692 144 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 398 607 936
Size: 398 209 329
Fragments arranged by size:
398 329 209
TatI w'GTAC_w
Cuts at: 0 176 701 936
Size: 176 525 235
Fragments arranged by size:
525 235 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 504 536 936
Size: 504 32 400
Fragments arranged by size:
504 400 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 612 936
Size: 612 324
XmnI GAAnn'nnTTC
Cuts at: 0 859 936
Size: 859 77
Enzymes that do cut:
AgeI AhdI AleI AlwNI ApaI ApoI BamHI BanI
BanII BbvCI BciVI BglI Bme1580I Bpu10I BpuEI BsaI
BsaWI BseRI BseYI BsgI Bsp1286I BspHI BspMI BsrFI
BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI EarI
EcoNI EcoO109I Hin4I HincII MmeI MscI MslI NcoI
PpuMI PshAI PspOMI PstI SexAI SfcI SfiI SmlI
StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbsI BcgI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI BpmI BsaAI BsaBI
BsaHI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI Eco57MI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XhoI XmaI ZraI