Restriction map for
pFN21AE5942
(Linear) (Six-base) MAPSORT of: KIEE5942.seq Check: 4029 from: 1 to: 936
KIEE5942.orf
With 182 enzymes: SgfI *
December 5, 2014 13:15 ..
AccI GT'mk_AC
Cuts at: 0 206 832 936
Size: 206 626 104
Fragments arranged by size:
626 206 104
AflIII A'CryG_T
Cuts at: 0 476 887 936
Size: 476 411 49
Fragments arranged by size:
476 411 49
ApoI r'AATT_y
Cuts at: 0 31 338 518 680 936
Size: 31 307 180 162 256
Fragments arranged by size:
307 256 180 162 31
BamHI G'GATC_C
Cuts at: 0 57 936
Size: 57 879
BciVI GTATCCnnnnn_n'
Cuts at: 0 914 936
Size: 914 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 786 936
Size: 11 775 150
Fragments arranged by size:
775 150 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 936
Size: 538 398
BsaWI w'CCGG_w
Cuts at: 0 60 936
Size: 60 876
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 139 169 936
Size: 139 30 767
Fragments arranged by size:
767 139 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 175 657 789 936
Size: 175 482 132 147
Fragments arranged by size:
482 175 147 132
BseYI C'CCAG_C
Cuts at: 0 117 936
Size: 117 819
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 936
Size: 457 479
BspEI T'CCGG_A
Cuts at: 0 60 936
Size: 60 876
BspHI T'CATG_A
Cuts at: 0 347 404 674 936
Size: 347 57 270 262
Fragments arranged by size:
347 270 262 57
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 936
Size: 134 802
BstEII G'GTnAC_C
Cuts at: 0 603 840 936
Size: 603 237 96
Fragments arranged by size:
603 237 96
BstXI CCAn_nnnn'nTGG
Cuts at: 0 105 117 243 936
Size: 105 12 126 693
Fragments arranged by size:
693 126 105 12
BstYI r'GATC_y
Cuts at: 0 57 936
Size: 57 879
BstZ17I GTA'TAC
Cuts at: 0 833 936
Size: 833 103
BtgI C'CryG_G
Cuts at: 0 836 936
Size: 836 100
ClaI AT'CG_AT
Cuts at: 0 363 936
Size: 363 573
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 402 437 936
Size: 402 35 499
Fragments arranged by size:
499 402 35
EaeI y'GGCC_r
Cuts at: 0 107 140 371 936
Size: 107 33 231 565
Fragments arranged by size:
565 231 107 33
EarI CTCTTCn'nnn_
Cuts at: 0 85 936
Size: 85 851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 786 936
Size: 11 775 150
Fragments arranged by size:
775 150 11
EcoRI G'AATT_C
Cuts at: 0 31 338 936
Size: 31 307 598
Fragments arranged by size:
598 307 31
HincII GTy'rAC
Cuts at: 0 147 207 936
Size: 147 60 729
Fragments arranged by size:
729 147 60
HindIII A'AGCT_T
Cuts at: 0 707 936
Size: 707 229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 936
Size: 303 633
MscI TGG'CCA
Cuts at: 0 109 373 936
Size: 109 264 563
Fragments arranged by size:
563 264 109
MslI CAynn'nnrTG
Cuts at: 0 172 229 481 592 847 936
Size: 172 57 252 111 255 89
Fragments arranged by size:
255 252 172 111 89 57
MspA1I CmG'CkG
Cuts at: 0 495 691 936
Size: 495 196 245
Fragments arranged by size:
495 245 196
NspI r_CATG'y
Cuts at: 0 891 936
Size: 891 45
PciI A'CATG_T
Cuts at: 0 887 936
Size: 887 49
PflMI CCAn_nnn'nTGG
Cuts at: 0 691 936
Size: 691 245
PfoI T'CCnGG_A
Cuts at: 0 458 802 936
Size: 458 344 134
Fragments arranged by size:
458 344 134
PstI C_TGCA'G
Cuts at: 0 71 936
Size: 71 865
PvuII CAG'CTG
Cuts at: 0 495 691 936
Size: 495 196 245
Fragments arranged by size:
495 245 196
SalI G'TCGA_C
Cuts at: 0 205 936
Size: 205 731
SfcI C'TryA_G
Cuts at: 0 67 490 936
Size: 67 423 446
Fragments arranged by size:
446 423 67
SmlI C'TyrA_G
Cuts at: 0 553 936
Size: 553 383
StyI C'CwwG_G
Cuts at: 0 200 936
Size: 200 736
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 310 398 936
Size: 310 88 538
Fragments arranged by size:
538 310 88
TatI w'GTAC_w
Cuts at: 0 176 936
Size: 176 760
XmnI GAAnn'nnTTC
Cuts at: 0 35 936
Size: 35 901
Enzymes that do cut:
AccI AflIII ApoI BamHI BciVI BpmI BpuEI BsaWI
BsaXI BseRI BseYI BsgI BspEI BspHI BspMI BstEII
BstXI BstYI BstZ17I BtgI ClaI CspCI EaeI EarI
Eco57MI EcoRI HincII HindIII MmeI MscI MslI MspA1I
NspI PciI PflMI PfoI PstI PvuII SalI SfcI
SmlI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BanI BanII BbeI
BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI
BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
Bsu36I BtgZI BtsI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII Hin4I HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI