Restriction map for
pFN21AE5941
(Linear) (Six-base) MAPSORT of: KIEE5941.seq Check: 6835 from: 1 to: 936
KIEE5941.orf
With 182 enzymes: SgfI *
December 5, 2014 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 673 936
Size: 673 263
AccI GT'mk_AC
Cuts at: 0 832 936
Size: 832 104
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 936
Size: 697 239
AvaI C'yCGr_G
Cuts at: 0 259 936
Size: 259 677
BanI G'GyrC_C
Cuts at: 0 607 936
Size: 607 329
BanII G_rGCy'C
Cuts at: 0 900 936
Size: 900 36
BbvCI CC'TCA_GC
Cuts at: 0 94 187 936
Size: 94 93 749
Fragments arranged by size:
749 94 93
BclI T'GATC_A
Cuts at: 0 620 936
Size: 620 316
Bme1580I G_kGCm'C
Cuts at: 0 426 612 936
Size: 426 186 324
Fragments arranged by size:
426 324 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 936
Size: 36 900
Bpu10I CC'TnA_GC
Cuts at: 0 94 187 936
Size: 94 93 749
Fragments arranged by size:
749 94 93
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 67 736 936
Size: 28 39 669 200
Fragments arranged by size:
669 200 39 28
BseYI C'CCAG_C
Cuts at: 0 62 936
Size: 62 874
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 457 527 651 936
Size: 148 309 70 124 285
Fragments arranged by size:
309 285 148 124 70
Bsp1286I G_dGCh'C
Cuts at: 0 426 612 900 936
Size: 426 186 288 36
Fragments arranged by size:
426 288 186 36
BspHI T'CATG_A
Cuts at: 0 617 936
Size: 617 319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 673 936
Size: 134 539 263
Fragments arranged by size:
539 263 134
BsrBI CCG'CTC
Cuts at: 0 5 936
Size: 5 931
BsrDI GCAATG_nn'
Cuts at: 0 764 936
Size: 764 172
BstAPI GCAn_nnn'nTGC
Cuts at: 0 480 514 936
Size: 480 34 422
Fragments arranged by size:
480 422 34
BstXI CCAn_nnnn'nTGG
Cuts at: 0 117 243 936
Size: 117 126 693
Fragments arranged by size:
693 126 117
BstZ17I GTA'TAC
Cuts at: 0 833 936
Size: 833 103
Bsu36I CC'TnA_GG
Cuts at: 0 874 936
Size: 874 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 551 936
Size: 551 385
DrdI GACnn_nn'nnGTC
Cuts at: 0 15 367 936
Size: 15 352 569
Fragments arranged by size:
569 352 15
EaeI y'GGCC_r
Cuts at: 0 107 245 936
Size: 107 138 691
Fragments arranged by size:
691 138 107
EarI CTCTTCn'nnn_
Cuts at: 0 754 936
Size: 754 182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 697 936
Size: 36 661 239
Fragments arranged by size:
661 239 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 583 601 936
Size: 583 18 335
Fragments arranged by size:
583 335 18
EcoO109I rG'GnC_Cy
Cuts at: 0 681 936
Size: 681 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 396 428 936
Size: 396 32 508
Fragments arranged by size:
508 396 32
MscI TGG'CCA
Cuts at: 0 109 247 936
Size: 109 138 689
Fragments arranged by size:
689 138 109
MslI CAynn'nnrTG
Cuts at: 0 172 298 319 581 769 936
Size: 172 126 21 262 188 167
Fragments arranged by size:
262 188 172 167 126 21
MspA1I CmG'CkG
Cuts at: 0 802 936
Size: 802 134
NspI r_CATG'y
Cuts at: 0 102 936
Size: 102 834
PpuMI rG'GwC_Cy
Cuts at: 0 681 936
Size: 681 255
PvuII CAG'CTG
Cuts at: 0 802 936
Size: 802 134
SfcI C'TryA_G
Cuts at: 0 753 867 936
Size: 753 114 69
Fragments arranged by size:
753 114 69
SmlI C'TyrA_G
Cuts at: 0 259 936
Size: 259 677
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 435 936
Size: 435 501
TatI w'GTAC_w
Cuts at: 0 176 936
Size: 176 760
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 431 463 936
Size: 431 32 473
Fragments arranged by size:
473 431 32
XhoI C'TCGA_G
Cuts at: 0 259 936
Size: 259 677
XmnI GAAnn'nnTTC
Cuts at: 0 709 936
Size: 709 227
Enzymes that do cut:
AarI AccI AcuI AvaI BanI BanII BbvCI BclI
Bme1580I BpmI Bpu10I BseRI BseYI BsgI Bsp1286I BspHI
BspMI BsrBI BsrDI BstAPI BstXI BstZ17I Bsu36I BtgZI
DrdI EaeI EarI Eco57MI EcoNI EcoO109I Hin4I MscI
MslI MspA1I NspI PpuMI PvuII SfcI SmlI TaqII
TatI TstI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BcgI
BciVI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstYI BtgI BtsI ClaI
CspCI DraI DraIII EagI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI Tth111I XbaI XcmI XmaI ZraI