Restriction map for
pFN21AE5936
(Linear) (Six-base) MAPSORT of: KIEE5936.seq Check: 9933 from: 1 to: 933
KIEE5936.orf
With 182 enzymes: SgfI *
December 5, 2014 13:15 ..
AccI GT'mk_AC
Cuts at: 0 593 933
Size: 593 340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 933
Size: 916 17
AflIII A'CryG_T
Cuts at: 0 572 933
Size: 572 361
AgeI A'CCGG_T
Cuts at: 0 362 933
Size: 362 571
AlwNI CAG_nnn'CTG
Cuts at: 0 118 503 933
Size: 118 385 430
Fragments arranged by size:
430 385 118
ApaLI G'TGCA_C
Cuts at: 0 324 933
Size: 324 609
AvaI C'yCGr_G
Cuts at: 0 779 933
Size: 779 154
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 286 319 933
Size: 286 33 614
Fragments arranged by size:
614 286 33
BanII G_rGCy'C
Cuts at: 0 419 933
Size: 419 514
BbvCI CC'TCA_GC
Cuts at: 0 496 933
Size: 496 437
BclI T'GATC_A
Cuts at: 0 128 933
Size: 128 805
BfrBI ATG'CAT
Cuts at: 0 659 680 933
Size: 659 21 253
Fragments arranged by size:
659 253 21
BglI GCCn_nnn'nGGC
Cuts at: 0 352 933
Size: 352 581
BglII A'GATC_T
Cuts at: 0 476 925 933
Size: 476 449 8
Fragments arranged by size:
476 449 8
Bme1580I G_kGCm'C
Cuts at: 0 236 328 933
Size: 236 92 605
Fragments arranged by size:
605 236 92
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 933
Size: 530 403
Bpu10I CC'TnA_GC
Cuts at: 0 496 933
Size: 496 437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 933
Size: 81 852
BsaI GGTCTCn'nnnn_
Cuts at: 0 202 933
Size: 202 731
BsaBI GATnn'nnATC
Cuts at: 0 133 933
Size: 133 800
BsaWI w'CCGG_w
Cuts at: 0 362 933
Size: 362 571
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 48 78 199 229 472 502 933
Size: 48 30 121 30 243 30 431
Fragments arranged by size:
431 243 121 48 30 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 549 933
Size: 148 401 384
Fragments arranged by size:
401 384 148
BsiHKAI G_wGCw'C
Cuts at: 0 328 933
Size: 328 605
BsmI GAATG_Cn'
Cuts at: 0 659 933
Size: 659 274
Bsp1286I G_dGCh'C
Cuts at: 0 236 328 419 933
Size: 236 92 91 514
Fragments arranged by size:
514 236 92 91
BsrDI GCAATG_nn'
Cuts at: 0 311 933
Size: 311 622
BsrFI r'CCGG_y
Cuts at: 0 362 933
Size: 362 571
BssSI C'ACGA_G
Cuts at: 0 20 933
Size: 20 913
BstEII G'GTnAC_C
Cuts at: 0 735 933
Size: 735 198
BstYI r'GATC_y
Cuts at: 0 476 925 933
Size: 476 449 8
Fragments arranged by size:
476 449 8
BtgI C'CryG_G
Cuts at: 0 350 411 933
Size: 350 61 522
Fragments arranged by size:
522 350 61
BtsI GCAGTG_nn'
Cuts at: 0 160 933
Size: 160 773
EaeI y'GGCC_r
Cuts at: 0 157 188 344 371 665 933
Size: 157 31 156 27 294 268
Fragments arranged by size:
294 268 157 156 31 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 415 933
Size: 415 518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 916 933
Size: 530 386 17
Fragments arranged by size:
530 386 17
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 498 933
Size: 498 435
MscI TGG'CCA
Cuts at: 0 190 373 667 933
Size: 190 183 294 266
Fragments arranged by size:
294 266 190 183
MslI CAynn'nnrTG
Cuts at: 0 40 223 571 933
Size: 40 183 348 362
Fragments arranged by size:
362 348 183 40
NcoI C'CATG_G
Cuts at: 0 350 411 933
Size: 350 61 522
Fragments arranged by size:
522 350 61
NsiI A_TGCA'T
Cuts at: 0 661 682 933
Size: 661 21 251
Fragments arranged by size:
661 251 21
NspI r_CATG'y
Cuts at: 0 576 684 933
Size: 576 108 249
Fragments arranged by size:
576 249 108
PasI CC'CwG_GG
Cuts at: 0 52 933
Size: 52 881
PciI A'CATG_T
Cuts at: 0 572 933
Size: 572 361
PflMI CCAn_nnn'nTGG
Cuts at: 0 729 933
Size: 729 204
PshAI GACnn'nnGTC
Cuts at: 0 22 933
Size: 22 911
PspXI vC'TCGA_Gb
Cuts at: 0 779 933
Size: 779 154
ScaI AGT'ACT
Cuts at: 0 551 933
Size: 551 382
SexAI A'CCwGG_T
Cuts at: 0 211 933
Size: 211 722
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 352 933
Size: 352 581
SmlI C'TyrA_G
Cuts at: 0 60 779 933
Size: 60 719 154
Fragments arranged by size:
719 154 60
SphI G_CATG'C
Cuts at: 0 684 933
Size: 684 249
StyI C'CwwG_G
Cuts at: 0 350 411 933
Size: 350 61 522
Fragments arranged by size:
522 350 61
TatI w'GTAC_w
Cuts at: 0 176 302 549 933
Size: 176 126 247 384
Fragments arranged by size:
384 247 176 126
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 36 585 933
Size: 36 549 348
Fragments arranged by size:
549 348 36
XhoI C'TCGA_G
Cuts at: 0 779 933
Size: 779 154
XmnI GAAnn'nnTTC
Cuts at: 0 703 933
Size: 703 230
Enzymes that do cut:
AccI AcuI AflIII AgeI AlwNI ApaLI AvaI BaeI
BanII BbvCI BclI BfrBI BglI BglII Bme1580I BpmI
Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BsgI BsiHKAI
BsmI Bsp1286I BsrDI BsrFI BssSI BstEII BstYI BtgI
BtsI EaeI EciI Eco57MI FalI MscI MslI NcoI
NsiI NspI PasI PciI PflMI PshAI PspXI ScaI
SexAI SfiI SmlI SphI StyI TatI XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AhdI AleI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII
BamHI BanI BbeI BbsI BcgI BciVI BlpI BmgBI
BmrI BmtI BplI BsaAI BsaHI BseRI BseYI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII
BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI SfcI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TaqII TstI Tth111I
XbaI XmaI ZraI