Restriction map for
pFN21AE5933
(Linear) (Six-base) MAPSORT of: KIEE5933.seq Check: 4209 from: 1 to: 933
KIEE5933.orf
With 182 enzymes: SgfI *
December 5, 2014 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 443 933
Size: 443 490
AccI GT'mk_AC
Cuts at: 0 390 933
Size: 390 543
Acc65I G'GTAC_C
Cuts at: 0 504 933
Size: 504 429
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 933
Size: 694 239
AflIII A'CryG_T
Cuts at: 0 727 933
Size: 727 206
AlwNI CAG_nnn'CTG
Cuts at: 0 252 933
Size: 252 681
ApaI G_GGCC'C
Cuts at: 0 271 933
Size: 271 662
ApoI r'AATT_y
Cuts at: 0 804 933
Size: 804 129
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 496 529 572 605 933
Size: 496 33 43 33 328
Fragments arranged by size:
496 328 43 33 33
BanI G'GyrC_C
Cuts at: 0 504 664 933
Size: 504 160 269
Fragments arranged by size:
504 269 160
BanII G_rGCy'C
Cuts at: 0 271 738 933
Size: 271 467 195
Fragments arranged by size:
467 271 195
BclI T'GATC_A
Cuts at: 0 608 933
Size: 608 325
Bme1580I G_kGCm'C
Cuts at: 0 271 933
Size: 271 662
BmrI ACTGGGnnnn_n'
Cuts at: 0 337 713 933
Size: 337 376 220
Fragments arranged by size:
376 337 220
BmtI G_CTAG'C
Cuts at: 0 29 933
Size: 29 904
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 933
Size: 772 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 794 933
Size: 794 139
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 148 178 933
Size: 148 30 755
Fragments arranged by size:
755 148 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 933
Size: 157 776
BsiHKAI G_wGCw'C
Cuts at: 0 738 933
Size: 738 195
Bsp1286I G_dGCh'C
Cuts at: 0 271 738 933
Size: 271 467 195
Fragments arranged by size:
467 271 195
BspHI T'CATG_A
Cuts at: 0 605 933
Size: 605 328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 443 933
Size: 443 490
BsrDI GCAATG_nn'
Cuts at: 0 827 933
Size: 827 106
BstYI r'GATC_y
Cuts at: 0 216 933
Size: 216 717
BtsI GCAGTG_nn'
Cuts at: 0 914 933
Size: 914 19
DraIII CAC_nnn'GTG
Cuts at: 0 341 933
Size: 341 592
EaeI y'GGCC_r
Cuts at: 0 770 933
Size: 770 163
EarI CTCTTCn'nnn_
Cuts at: 0 907 933
Size: 907 26
EcoICRI GAG'CTC
Cuts at: 0 736 933
Size: 736 197
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 772 933
Size: 694 78 161
Fragments arranged by size:
694 161 78
EcoO109I rG'GnC_Cy
Cuts at: 0 267 933
Size: 267 666
HincII GTy'rAC
Cuts at: 0 698 933
Size: 698 235
KpnI G_GTAC'C
Cuts at: 0 508 933
Size: 508 425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 933
Size: 33 900
MslI CAynn'nnrTG
Cuts at: 0 181 590 933
Size: 181 409 343
Fragments arranged by size:
409 343 181
MspA1I CmG'CkG
Cuts at: 0 382 933
Size: 382 551
NheI G'CTAG_C
Cuts at: 0 25 933
Size: 25 908
NspI r_CATG'y
Cuts at: 0 731 933
Size: 731 202
PciI A'CATG_T
Cuts at: 0 727 933
Size: 727 206
PflMI CCAn_nnn'nTGG
Cuts at: 0 438 933
Size: 438 495
PspOMI G'GGCC_C
Cuts at: 0 267 933
Size: 267 666
PvuII CAG'CTG
Cuts at: 0 382 933
Size: 382 551
SacI G_AGCT'C
Cuts at: 0 738 933
Size: 738 195
SmlI C'TyrA_G
Cuts at: 0 809 933
Size: 809 124
TatI w'GTAC_w
Cuts at: 0 185 681 933
Size: 185 496 252
Fragments arranged by size:
496 252 185
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlwNI ApaI ApoI
BaeI BanI BanII BclI Bme1580I BmrI BmtI BpmI
BpuEI BsaXI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI
BstYI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I
HincII KpnI MmeI MslI MspA1I NheI NspI PciI
PflMI PspOMI PvuII SacI SmlI TatI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvaI AvrII BamHI
BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII
BlpI BmgBI BplI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HindIII HpaI KasI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NotI
NruI NsiI PacI PasI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI