Restriction map for
pFN21AE5931
(Linear) (Six-base) MAPSORT of: KIEE5931.seq Check: 7420 from: 1 to: 933
KIEE5931.orf
With 182 enzymes: SgfI *
December 5, 2014 13:14 ..
AccI GT'mk_AC
Cuts at: 0 443 933
Size: 443 490
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 922 933
Size: 5 917 11
Fragments arranged by size:
917 11 5
AleI CACnn'nnGTG
Cuts at: 0 115 933
Size: 115 818
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 259 291 933
Size: 259 32 642
Fragments arranged by size:
642 259 32
AlwNI CAG_nnn'CTG
Cuts at: 0 28 419 933
Size: 28 391 514
Fragments arranged by size:
514 391 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 812 933
Size: 812 121
BciVI GTATCCnnnnn_n'
Cuts at: 0 682 933
Size: 682 251
BfrBI ATG'CAT
Cuts at: 0 290 933
Size: 290 643
BmrI ACTGGGnnnn_n'
Cuts at: 0 460 933
Size: 460 473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 552 933
Size: 155 397 381
Fragments arranged by size:
397 381 155
Bpu10I CC'TnA_GC
Cuts at: 0 621 933
Size: 621 312
BsaI GGTCTCn'nnnn_
Cuts at: 0 515 933
Size: 515 418
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 68 98 259 289 933
Size: 68 30 161 30 644
Fragments arranged by size:
644 161 68 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 210 277 623 933
Size: 210 67 346 310
Fragments arranged by size:
346 310 210 67
BsmI GAATG_Cn'
Cuts at: 0 292 933
Size: 292 641
BstXI CCAn_nnnn'nTGG
Cuts at: 0 432 933
Size: 432 501
BstZ17I GTA'TAC
Cuts at: 0 444 933
Size: 444 489
Bsu36I CC'TnA_GG
Cuts at: 0 874 933
Size: 874 59
BtgI C'CryG_G
Cuts at: 0 350 933
Size: 350 583
BtsI GCAGTG_nn'
Cuts at: 0 385 933
Size: 385 548
EarI CTCTTCn'nnn_
Cuts at: 0 85 933
Size: 85 848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 155 552 922 933
Size: 5 150 397 370 11
Fragments arranged by size:
397 370 150 11 5
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 355 933
Size: 355 578
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 685 717 802 834 933
Size: 685 32 85 32 99
Fragments arranged by size:
685 99 85 32 32
MslI CAynn'nnrTG
Cuts at: 0 115 430 490 581 799 933
Size: 115 315 60 91 218 134
Fragments arranged by size:
315 218 134 115 91 60
NcoI C'CATG_G
Cuts at: 0 350 933
Size: 350 583
NsiI A_TGCA'T
Cuts at: 0 292 933
Size: 292 641
NspI r_CATG'y
Cuts at: 0 489 933
Size: 489 444
PflMI CCAn_nnn'nTGG
Cuts at: 0 735 933
Size: 735 198
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 933
Size: 259 32 642
Fragments arranged by size:
642 259 32
SfcI C'TryA_G
Cuts at: 0 504 867 933
Size: 504 363 66
Fragments arranged by size:
504 363 66
SspI AAT'ATT
Cuts at: 0 399 933
Size: 399 534
StyI C'CwwG_G
Cuts at: 0 93 350 675 933
Size: 93 257 325 258
Fragments arranged by size:
325 258 257 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 589 933
Size: 589 344
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 68 89 100 121 623 655 933
Size: 68 21 11 21 502 32 278
Fragments arranged by size:
502 278 68 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 0 558 933
Size: 558 375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 798 933
Size: 798 135
Enzymes that do cut:
AccI AcuI AleI AloI AlwNI BbsI BciVI BfrBI
BmrI BpmI Bpu10I BsaI BsaXI BseRI BsmI BstXI
BstZ17I Bsu36I BtgI BtsI EarI Eco57MI FalI Hin4I
MslI NcoI NsiI NspI PflMI PpiI SfcI SspI
StyI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmtI
BplI BpuEI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
SwaI TatI XbaI XhoI XmaI XmnI ZraI