Restriction map for
pFN21AE5929
(Linear) (Six-base) MAPSORT of: KIEE5929.seq Check: 9621 from: 1 to: 933
KIEE5929.orf
With 182 enzymes: SgfI *
December 5, 2014 13:14 ..
AfeI AGC'GCT
Cuts at: 0 455 933
Size: 455 478
AgeI A'CCGG_T
Cuts at: 0 359 933
Size: 359 574
AleI CACnn'nnGTG
Cuts at: 0 19 933
Size: 19 914
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 400 933
Size: 400 533
ApoI r'AATT_y
Cuts at: 0 68 933
Size: 68 865
AseI AT'TA_AT
Cuts at: 0 518 898 933
Size: 518 380 35
Fragments arranged by size:
518 380 35
BanI G'GyrC_C
Cuts at: 0 435 933
Size: 435 498
BglII A'GATC_T
Cuts at: 0 329 933
Size: 329 604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 933
Size: 262 671
Bpu10I CC'TnA_GC
Cuts at: 0 184 933
Size: 184 749
BsaI GGTCTCn'nnnn_
Cuts at: 0 466 933
Size: 466 467
BsaBI GATnn'nnATC
Cuts at: 0 334 933
Size: 334 599
BsaWI w'CCGG_w
Cuts at: 0 359 933
Size: 359 574
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 56 86 463 493 933
Size: 56 30 377 30 440
Fragments arranged by size:
440 377 56 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 933
Size: 64 869
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 933
Size: 313 620
BsiHKAI G_wGCw'C
Cuts at: 0 421 933
Size: 421 512
Bsp1286I G_dGCh'C
Cuts at: 0 421 933
Size: 421 512
BsrFI r'CCGG_y
Cuts at: 0 359 933
Size: 359 574
BstXI CCAn_nnnn'nTGG
Cuts at: 0 102 933
Size: 102 831
BstYI r'GATC_y
Cuts at: 0 204 329 933
Size: 204 125 604
Fragments arranged by size:
604 204 125
BtsI GCAGTG_nn'
Cuts at: 0 301 933
Size: 301 632
DraI TTT'AAA
Cuts at: 0 674 933
Size: 674 259
DrdI GACnn_nn'nnGTC
Cuts at: 0 50 933
Size: 50 883
EaeI y'GGCC_r
Cuts at: 0 92 368 933
Size: 92 276 565
Fragments arranged by size:
565 276 92
EarI CTCTTCn'nnn_
Cuts at: 0 82 222 634 933
Size: 82 140 412 299
Fragments arranged by size:
412 299 140 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 933
Size: 262 671
EcoNI CCTnn'n_nnAGG
Cuts at: 0 142 933
Size: 142 791
HaeII r_GCGC'y
Cuts at: 0 457 933
Size: 457 476
HindIII A'AGCT_T
Cuts at: 0 704 933
Size: 704 229
MscI TGG'CCA
Cuts at: 0 94 370 933
Size: 94 276 563
Fragments arranged by size:
563 276 94
MslI CAynn'nnrTG
Cuts at: 0 19 100 169 433 933
Size: 19 81 69 264 500
Fragments arranged by size:
500 264 81 69 19
MspA1I CmG'CkG
Cuts at: 0 736 933
Size: 736 197
NdeI CA'TA_TG
Cuts at: 0 637 933
Size: 637 296
NspI r_CATG'y
Cuts at: 0 168 933
Size: 168 765
PflMI CCAn_nnn'nTGG
Cuts at: 0 155 933
Size: 155 778
PshAI GACnn'nnGTC
Cuts at: 0 736 933
Size: 736 197
PsiI TTA'TAA
Cuts at: 0 859 933
Size: 859 74
PvuII CAG'CTG
Cuts at: 0 736 933
Size: 736 197
SfcI C'TryA_G
Cuts at: 0 686 933
Size: 686 247
StyI C'CwwG_G
Cuts at: 0 123 314 933
Size: 123 191 619
Fragments arranged by size:
619 191 123
TatI w'GTAC_w
Cuts at: 0 173 933
Size: 173 760
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 463 495 933
Size: 463 32 438
Fragments arranged by size:
463 438 32
Enzymes that do cut:
AfeI AgeI AleI AlfI ApoI AseI BanI BglII
BpmI Bpu10I BsaI BsaBI BsaWI BsaXI BseRI BsgI
BsiHKAI Bsp1286I BsrFI BstXI BstYI BtsI DraI DrdI
EaeI EarI Eco57MI EcoNI HaeII HindIII MscI MslI
MspA1I NdeI NspI PflMI PshAI PsiI PvuII SfcI
StyI TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AhdI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BmtI BplI BpuEI BsaAI BsaHI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraIII EagI EciI EcoICRI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI