Restriction map for
pFN21AE5928
(Linear) (Six-base) MAPSORT of: KIEE5928.seq Check: 2926 from: 1 to: 933
KIEE5928.orf
With 182 enzymes: SgfI *
December 5, 2014 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 404 933
Size: 404 529
AflIII A'CryG_T
Cuts at: 0 203 933
Size: 203 730
AleI CACnn'nnGTG
Cuts at: 0 397 933
Size: 397 536
BamHI G'GATC_C
Cuts at: 0 677 933
Size: 677 256
BanI G'GyrC_C
Cuts at: 0 225 933
Size: 225 708
BbvCI CC'TCA_GC
Cuts at: 0 573 933
Size: 573 360
BclI T'GATC_A
Cuts at: 0 137 933
Size: 137 796
BglI GCCn_nnn'nGGC
Cuts at: 0 427 933
Size: 427 506
BlpI GC'TnA_GC
Cuts at: 0 293 933
Size: 293 640
Bme1580I G_kGCm'C
Cuts at: 0 230 423 933
Size: 230 193 510
Fragments arranged by size:
510 230 193
Bpu10I CC'TnA_GC
Cuts at: 0 385 573 933
Size: 385 188 360
Fragments arranged by size:
385 360 188
BsaAI yAC'GTr
Cuts at: 0 99 933
Size: 99 834
BsaBI GATnn'nnATC
Cuts at: 0 589 933
Size: 589 344
BsaHI Gr'CG_yC
Cuts at: 0 65 835 933
Size: 65 770 98
Fragments arranged by size:
770 98 65
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 402 432 933
Size: 402 30 501
Fragments arranged by size:
501 402 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 112 933
Size: 61 51 821
Fragments arranged by size:
821 61 51
BseYI C'CCAG_C
Cuts at: 0 111 933
Size: 111 822
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 584 901 933
Size: 442 142 317 32
Fragments arranged by size:
442 317 142 32
BsiHKAI G_wGCw'C
Cuts at: 0 104 325 933
Size: 104 221 608
Fragments arranged by size:
608 221 104
Bsp1286I G_dGCh'C
Cuts at: 0 104 230 325 423 933
Size: 104 126 95 98 510
Fragments arranged by size:
510 126 104 98 95
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 404 933
Size: 404 529
BsrBI CCG'CTC
Cuts at: 0 384 933
Size: 384 549
BsrFI r'CCGG_y
Cuts at: 0 433 933
Size: 433 500
BsrGI T'GTAC_A
Cuts at: 0 857 933
Size: 857 76
BssSI C'ACGA_G
Cuts at: 0 16 933
Size: 16 917
BstXI CCAn_nnnn'nTGG
Cuts at: 0 585 933
Size: 585 348
BstYI r'GATC_y
Cuts at: 0 515 677 933
Size: 515 162 256
Fragments arranged by size:
515 256 162
Bsu36I CC'TnA_GG
Cuts at: 0 618 933
Size: 618 315
BtgI C'CryG_G
Cuts at: 0 788 797 933
Size: 788 9 136
Fragments arranged by size:
788 136 9
DraIII CAC_nnn'GTG
Cuts at: 0 329 933
Size: 329 604
DrdI GACnn_nn'nnGTC
Cuts at: 0 249 933
Size: 249 684
EaeI y'GGCC_r
Cuts at: 0 368 428 812 933
Size: 368 60 384 121
Fragments arranged by size:
384 368 121 60
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 933
Size: 29 904
MscI TGG'CCA
Cuts at: 0 370 430 814 933
Size: 370 60 384 119
Fragments arranged by size:
384 370 119 60
MslI CAynn'nnrTG
Cuts at: 0 166 202 346 397 595 933
Size: 166 36 144 51 198 338
Fragments arranged by size:
338 198 166 144 51 36
MspA1I CmG'CkG
Cuts at: 0 284 933
Size: 284 649
NcoI C'CATG_G
Cuts at: 0 788 797 933
Size: 788 9 136
Fragments arranged by size:
788 136 9
NspI r_CATG'y
Cuts at: 0 207 933
Size: 207 726
PasI CC'CwG_GG
Cuts at: 0 55 933
Size: 55 878
PciI A'CATG_T
Cuts at: 0 203 933
Size: 203 730
PflMI CCAn_nnn'nTGG
Cuts at: 0 315 933
Size: 315 618
PvuII CAG'CTG
Cuts at: 0 284 933
Size: 284 649
StuI AGG'CCT
Cuts at: 0 41 933
Size: 41 892
StyI C'CwwG_G
Cuts at: 0 245 788 797 933
Size: 245 543 9 136
Fragments arranged by size:
543 245 136 9
TatI w'GTAC_w
Cuts at: 0 170 857 933
Size: 170 687 76
Fragments arranged by size:
687 170 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 59 402 434 530 562 933
Size: 27 32 343 32 96 32 371
Fragments arranged by size:
371 343 96 32 32 32 27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 552 795 933
Size: 552 243 138
Fragments arranged by size:
552 243 138
Enzymes that do cut:
AarI AflIII AleI BamHI BanI BbvCI BclI BglI
BlpI Bme1580I Bpu10I BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI
BssSI BstXI BstYI Bsu36I BtgI DraIII DrdI EaeI
MmeI MscI MslI MspA1I NcoI NspI PasI PciI
PflMI PvuII StuI StyI TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BanII BbeI BbsI
BcgI BciVI BfrBI BglII BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BssHII BstAPI BstBI BstEII BstZ17I
BtgZI BtsI ClaI CspCI DraI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I
XbaI XhoI XmaI XmnI ZraI