Restriction map for
pFN21AE5926
(Linear) (Six-base) MAPSORT of: KIEE5926.seq Check: 7497 from: 1 to: 933
KIEE5926.orf
With 182 enzymes: SgfI *
December 5, 2014 13:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 933
Size: 922 11
AhdI GACnn_n'nnGTC
Cuts at: 0 255 933
Size: 255 678
AleI CACnn'nnGTG
Cuts at: 0 736 933
Size: 736 197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 440 933
Size: 440 493
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 500 532 933
Size: 500 32 401
Fragments arranged by size:
500 401 32
ApoI r'AATT_y
Cuts at: 0 31 933
Size: 31 902
BbsI GAAGACnn'nnnn_
Cuts at: 0 903 915 933
Size: 903 12 18
Fragments arranged by size:
903 18 12
BbvCI CC'TCA_GC
Cuts at: 0 673 933
Size: 673 260
BglII A'GATC_T
Cuts at: 0 476 933
Size: 476 457
Bme1580I G_kGCm'C
Cuts at: 0 758 933
Size: 758 175
Bpu10I CC'TnA_GC
Cuts at: 0 673 933
Size: 673 260
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 933
Size: 604 329
BsaI GGTCTCn'nnnn_
Cuts at: 0 683 933
Size: 683 250
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 216 246 933
Size: 216 30 687
Fragments arranged by size:
687 216 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 210 933
Size: 118 92 723
Fragments arranged by size:
723 118 92
BseYI C'CCAG_C
Cuts at: 0 117 933
Size: 117 816
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 493 933
Size: 373 120 440
Fragments arranged by size:
440 373 120
Bsp1286I G_dGCh'C
Cuts at: 0 758 933
Size: 758 175
BspHI T'CATG_A
Cuts at: 0 404 933
Size: 404 529
BsrDI GCAATG_nn'
Cuts at: 0 424 933
Size: 424 509
BsrGI T'GTAC_A
Cuts at: 0 101 933
Size: 101 832
BssSI C'ACGA_G
Cuts at: 0 913 933
Size: 913 20
BstAPI GCAn_nnn'nTGC
Cuts at: 0 753 933
Size: 753 180
BstEII G'GTnAC_C
Cuts at: 0 138 738 933
Size: 138 600 195
Fragments arranged by size:
600 195 138
BstXI CCAn_nnnn'nTGG
Cuts at: 0 319 933
Size: 319 614
BstYI r'GATC_y
Cuts at: 0 476 933
Size: 476 457
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 551 933
Size: 551 382
BtsI GCAGTG_nn'
Cuts at: 0 422 785 933
Size: 422 363 148
Fragments arranged by size:
422 363 148
DraIII CAC_nnn'GTG
Cuts at: 0 396 933
Size: 396 537
DrdI GACnn_nn'nnGTC
Cuts at: 0 153 675 933
Size: 153 522 258
Fragments arranged by size:
522 258 153
EaeI y'GGCC_r
Cuts at: 0 371 933
Size: 371 562
EarI CTCTTCn'nnn_
Cuts at: 0 313 898 933
Size: 313 585 35
Fragments arranged by size:
585 313 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 933
Size: 922 11
EcoO109I rG'GnC_Cy
Cuts at: 0 549 933
Size: 549 384
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 846 878 933
Size: 241 32 573 32 55
Fragments arranged by size:
573 241 55 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 933
Size: 626 307
MscI TGG'CCA
Cuts at: 0 373 933
Size: 373 560
MslI CAynn'nnrTG
Cuts at: 0 172 664 733 736 933
Size: 172 492 69 3 197
Fragments arranged by size:
492 197 172 69 3
PflMI CCAn_nnn'nTGG
Cuts at: 0 72 933
Size: 72 861
PpuMI rG'GwC_Cy
Cuts at: 0 549 933
Size: 549 384
SmlI C'TyrA_G
Cuts at: 0 583 933
Size: 583 350
StyI C'CwwG_G
Cuts at: 0 318 933
Size: 318 615
TatI w'GTAC_w
Cuts at: 0 101 176 933
Size: 101 75 757
Fragments arranged by size:
757 101 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 216 248 933
Size: 216 32 685
Fragments arranged by size:
685 216 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 319 609 933
Size: 319 290 324
Fragments arranged by size:
324 319 290
Enzymes that do cut:
AcuI AhdI AleI AlfI AloI ApoI BbsI BbvCI
BglII Bme1580I Bpu10I BpuEI BsaI BsaXI BseRI BseYI
BsgI Bsp1286I BspHI BsrDI BsrGI BssSI BstAPI BstEII
BstXI BstYI BtgZI BtsI DraIII DrdI EaeI EarI
Eco57MI EcoO109I Hin4I MmeI MscI MslI PflMI PpuMI
SmlI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BcgI BciVI
BclI BfrBI BglI BlpI BmgBI BmrI BmtI BplI
BpmI BsaAI BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrFI BssHII BstBI
BstZ17I Bsu36I BtgI ClaI CspCI DraI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XhoI XmaI XmnI ZraI