(Linear) (Six-base) MAPSORT of: KIEE5925.seq  Check: 5046  from: 1  to: 933



KIEE5925.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:14  ..

AccI GT'mk_AC

Cuts at:      0     712     933 
   Size:        712     221
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     674     933 
   Size:         80     594     259

  Fragments arranged by size: 

                 594     259      80
 
AflIII A'CryG_T

Cuts at:      0     536     933 
   Size:        536     397
 
AleI CACnn'nnGTG

Cuts at:      0      22     933 
   Size:         22     911
 
AlwNI CAG_nnn'CTG

Cuts at:      0      58     933 
   Size:         58     875
 
ApaI G_GGCC'C

Cuts at:      0     440     933 
   Size:        440     493
 
AseI AT'TA_AT

Cuts at:      0     680     933 
   Size:        680     253
 
BanII G_rGCy'C

Cuts at:      0     440     933 
   Size:        440     493
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     303     924     933 
   Size:        303     621       9

  Fragments arranged by size: 

                 621     303       9
 
Bme1580I G_kGCm'C

Cuts at:      0     440     933 
   Size:        440     493
 
BmgBI CAC'GTC

Cuts at:      0     539     933 
   Size:        539     394
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     388     443     933 
   Size:        388      55     490

  Fragments arranged by size: 

                 490     388      55
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     912     933 
   Size:        142     770      21

  Fragments arranged by size: 

                 770     142      21
 
Bpu10I CC'TnA_GC

Cuts at:      0     243     933 
   Size:        243     690
 
BsaAI yAC'GTr

Cuts at:      0     366     933 
   Size:        366     567
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      77     107     933 
   Size:         77      30     826

  Fragments arranged by size: 

                 826      77      30
 
BseYI C'CCAG_C

Cuts at:      0     117     933 
   Size:        117     816
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     545     933 
   Size:        545     388
 
Bsp1286I G_dGCh'C

Cuts at:      0     440     933 
   Size:        440     493
 
BspHI T'CATG_A

Cuts at:      0     587     933 
   Size:        587     346
 
BsrDI GCAATG_nn'

Cuts at:      0     129     933 
   Size:        129     804
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     618     933 
   Size:        618     315
 
DraI TTT'AAA

Cuts at:      0     776     933 
   Size:        776     157
 
EarI CTCTTCn'nnn_

Cuts at:      0      56     933 
   Size:         56     877
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     136     933 
   Size:        136     797
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     142     674     912     933 
   Size:         80      62     532     238      21

  Fragments arranged by size: 

                 532     238      80      62      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     437     933 
   Size:        437     496
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     806     933 
   Size:        806     127
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     728     760     933 
   Size:        728      32     173

  Fragments arranged by size: 

                 728     173      32
 
HindIII A'AGCT_T

Cuts at:      0     704     933 
   Size:        704     229
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     287     933 
   Size:        287     646
 
MslI CAynn'nnrTG

Cuts at:      0      22     172     616     933 
   Size:         22     150     444     317

  Fragments arranged by size: 

                 444     317     150      22
 
PspOMI G'GGCC_C

Cuts at:      0     436     933 
   Size:        436     497
 
PstI C_TGCA'G

Cuts at:      0     379     933 
   Size:        379     554
 
SfcI C'TryA_G

Cuts at:      0     375     933 
   Size:        375     558
 
SnaBI TAC'GTA

Cuts at:      0     366     933 
   Size:        366     567
 
XmnI GAAnn'nnTTC

Cuts at:      0     332     933 
   Size:        332     601
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI    AlwNI     ApaI     AseI    BanII
     BbsI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BsaAI    BsaXI
    BseYI    BsmBI Bsp1286I    BspHI    BsrDI    BstXI     DraI     EarI
     EciI  Eco57MI EcoO109I     FalI    Hin4I  HindIII     MmeI     MslI
   PspOMI     PstI     SfcI    SnaBI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI    ApaLI     ApoI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI     BmtI     BplI    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI