(Linear) (Six-base) MAPSORT of: KIEE5918.seq  Check: 4331  from: 1  to: 930



KIEE5918.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     930 
   Size:        256     674
 
ApaI G_GGCC'C

Cuts at:      0     761     930 
   Size:        761     169
 
ApoI r'AATT_y

Cuts at:      0     192     930 
   Size:        192     738
 
AseI AT'TA_AT

Cuts at:      0     433     930 
   Size:        433     497
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     286     319     930 
   Size:        286      33     611

  Fragments arranged by size: 

                 611     286      33
 
BanII G_rGCy'C

Cuts at:      0     729     761     784     930 
   Size:        729      32      23     146

  Fragments arranged by size: 

                 729     146      32      23
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     448     930 
   Size:        448     482
 
BclI T'GATC_A

Cuts at:      0     245     930 
   Size:        245     685
 
Bme1580I G_kGCm'C

Cuts at:      0     761     930 
   Size:        761     169
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     748     930 
   Size:        748     182
 
BsaBI GATnn'nnATC

Cuts at:      0     244     930 
   Size:        244     686
 
BsiHKAI G_wGCw'C

Cuts at:      0     729     930 
   Size:        729     201
 
BsmI GAATG_Cn'

Cuts at:      0      88     292     930 
   Size:         88     204     638

  Fragments arranged by size: 

                 638     204      88
 
Bsp1286I G_dGCh'C

Cuts at:      0     729     761     784     930 
   Size:        729      32      23     146

  Fragments arranged by size: 

                 729     146      32      23
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     598     930 
   Size:        598     332
 
BsrDI GCAATG_nn'

Cuts at:      0      19     930 
   Size:         19     911
 
BtgI C'CryG_G

Cuts at:      0     326     930 
   Size:        326     604
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      46      81     930 
   Size:         46      35     849

  Fragments arranged by size: 

                 849      46      35
 
EcoICRI GAG'CTC

Cuts at:      0     727     930 
   Size:        727     203
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     930 
   Size:        256     674
 
EcoO109I rG'GnC_Cy

Cuts at:      0     758     930 
   Size:        758     172
 
EcoRV GAT'ATC

Cuts at:      0     275     930 
   Size:        275     655
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     261     293     407     439     807     839     930 
   Size:        261      32     114      32     368      32      91

  Fragments arranged by size: 

                 368     261     114      91      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     536     930 
   Size:        536     394
 
MslI CAynn'nnrTG

Cuts at:      0     115     775     907     930 
   Size:        115     660     132      23

  Fragments arranged by size: 

                 660     132     115      23
 
NdeI CA'TA_TG

Cuts at:      0     357     376     444     930 
   Size:        357      19      68     486

  Fragments arranged by size: 

                 486     357      68      19
 
PspOMI G'GGCC_C

Cuts at:      0     757     930 
   Size:        757     173
 
SacI G_AGCT'C

Cuts at:      0     729     930 
   Size:        729     201
 
SfcI C'TryA_G

Cuts at:      0     867     930 
   Size:        867      63
 
StyI C'CwwG_G

Cuts at:      0     186     930 
   Size:        186     744
 
TatI w'GTAC_w

Cuts at:      0     176     930 
   Size:        176     754
 
XmnI GAAnn'nnTTC

Cuts at:      0     335     930 
   Size:        335     595
 

 Enzymes that do cut:

     AcuI     ApaI     ApoI     AseI     BaeI    BanII     BbsI     BclI
 Bme1580I     BplI    BsaBI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI
     BtgI    CspCI  EcoICRI  Eco57MI EcoO109I    EcoRV    Hin4I     MmeI
     MslI     NdeI   PspOMI     SacI     SfcI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI
    AsiSI     AvaI    AvrII    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI    EcoNI    EcoRI
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI