Restriction map for
pFN21AE5917
(Linear) (Six-base) MAPSORT of: KIEE5917.seq Check: 8802 from: 1 to: 930
KIEE5917.orf
With 182 enzymes: SgfI *
December 5, 2014 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 724 930
Size: 724 206
AccI GT'mk_AC
Cuts at: 0 203 930
Size: 203 727
AflIII A'CryG_T
Cuts at: 0 445 930
Size: 445 485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 244 930
Size: 244 686
AlwNI CAG_nnn'CTG
Cuts at: 0 240 930
Size: 240 690
ApaI G_GGCC'C
Cuts at: 0 58 930
Size: 58 872
BamHI G'GATC_C
Cuts at: 0 677 930
Size: 677 253
BanI G'GyrC_C
Cuts at: 0 228 892 930
Size: 228 664 38
Fragments arranged by size:
664 228 38
BanII G_rGCy'C
Cuts at: 0 58 86 140 930
Size: 58 28 54 790
Fragments arranged by size:
790 58 54 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 234 268 930
Size: 234 34 662
Fragments arranged by size:
662 234 34
Bme1580I G_kGCm'C
Cuts at: 0 58 233 897 930
Size: 58 175 664 33
Fragments arranged by size:
664 175 58 33
BmgBI CAC'GTC
Cuts at: 0 446 930
Size: 446 484
BmrI ACTGGGnnnn_n'
Cuts at: 0 49 919 930
Size: 49 870 11
Fragments arranged by size:
870 49 11
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 429 930
Size: 138 291 501
Fragments arranged by size:
501 291 138
BsaAI yAC'GTr
Cuts at: 0 366 930
Size: 366 564
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 711 814 915 930
Size: 64 647 103 101 15
Fragments arranged by size:
647 103 101 64 15
BseYI C'CCAG_C
Cuts at: 0 114 628 930
Size: 114 514 302
Fragments arranged by size:
514 302 114
BsiEI CG_ry'CG
Cuts at: 0 360 930
Size: 360 570
BsiHKAI G_wGCw'C
Cuts at: 0 909 930
Size: 909 21
Bsp1286I G_dGCh'C
Cuts at: 0 58 86 140 233 897 909 930
Size: 58 28 54 93 664 12 21
Fragments arranged by size:
664 93 58 54 28 21 12
BspHI T'CATG_A
Cuts at: 0 401 510 930
Size: 401 109 420
Fragments arranged by size:
420 401 109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 724 930
Size: 724 206
BsrGI T'GTAC_A
Cuts at: 0 770 930
Size: 770 160
BstYI r'GATC_y
Cuts at: 0 677 930
Size: 677 253
Bsu36I CC'TnA_GG
Cuts at: 0 550 673 797 871 898 930
Size: 550 123 124 74 27 32
Fragments arranged by size:
550 124 123 74 32 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 192 930
Size: 192 738
BtsI GCAGTG_nn'
Cuts at: 0 762 930
Size: 762 168
EaeI y'GGCC_r
Cuts at: 0 368 428 665 930
Size: 368 60 237 265
Fragments arranged by size:
368 265 237 60
EarI CTCTTCn'nnn_
Cuts at: 0 91 751 930
Size: 91 660 179
Fragments arranged by size:
660 179 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 429 930
Size: 138 291 501
Fragments arranged by size:
501 291 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 380 404 412 436 930
Size: 380 24 8 24 494
Fragments arranged by size:
494 380 24 24 8
HincII GTy'rAC
Cuts at: 0 204 930
Size: 204 726
MscI TGG'CCA
Cuts at: 0 370 930
Size: 370 560
MslI CAynn'nnrTG
Cuts at: 0 169 766 772 930
Size: 169 597 6 158
Fragments arranged by size:
597 169 158 6
PflMI CCAn_nnn'nTGG
Cuts at: 0 240 930
Size: 240 690
PfoI T'CCnGG_A
Cuts at: 0 439 930
Size: 439 491
PshAI GACnn'nnGTC
Cuts at: 0 19 930
Size: 19 911
PspOMI G'GGCC_C
Cuts at: 0 54 930
Size: 54 876
PvuI CG_AT'CG
Cuts at: 0 360 930
Size: 360 570
SalI G'TCGA_C
Cuts at: 0 202 930
Size: 202 728
SapI GCTCTTCn'nnn_
Cuts at: 0 91 930
Size: 91 839
SexAI A'CCwGG_T
Cuts at: 0 581 930
Size: 581 349
StuI AGG'CCT
Cuts at: 0 706 930
Size: 706 224
StyI C'CwwG_G
Cuts at: 0 58 930
Size: 58 872
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 642 930
Size: 642 288
TatI w'GTAC_w
Cuts at: 0 173 770 930
Size: 173 597 160
Fragments arranged by size:
597 173 160
Enzymes that do cut:
AarI AccI AflIII AlfI AlwNI ApaI BamHI BanI
BanII BcgI Bme1580I BmgBI BmrI BpmI BsaAI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrGI BstYI
Bsu36I BtgZI BtsI EaeI EarI Eco57MI Hin4I HincII
MscI MslI PflMI PfoI PshAI PspOMI PvuI SalI
SapI SexAI StuI StyI TaqII TatI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI BmtI BplI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BsgI BsiWI BsmI BsmBI
BspEI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PpuMI PsiI PspXI
PsrI PstI PvuII RsrII SacI SacII SanDI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI