Restriction map for
pFN21AE5916
(Linear) (Six-base) MAPSORT of: KIEE5916.seq Check: 6635 from: 1 to: 930
KIEE5916.orf
With 182 enzymes: SgfI *
December 5, 2014 13:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 930
Size: 277 653
AflIII A'CryG_T
Cuts at: 0 206 930
Size: 206 724
ApaLI G'TGCA_C
Cuts at: 0 417 930
Size: 417 513
AvaI C'yCGr_G
Cuts at: 0 219 930
Size: 219 711
BamHI G'GATC_C
Cuts at: 0 677 930
Size: 677 253
BanII G_rGCy'C
Cuts at: 0 140 897 930
Size: 140 757 33
Fragments arranged by size:
757 140 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 171 930
Size: 171 759
Bme1580I G_kGCm'C
Cuts at: 0 421 930
Size: 421 509
BmtI G_CTAG'C
Cuts at: 0 244 930
Size: 244 686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 429 930
Size: 85 344 501
Fragments arranged by size:
501 344 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 693 782 930
Size: 693 89 148
Fragments arranged by size:
693 148 89
BsaI GGTCTCn'nnnn_
Cuts at: 0 648 930
Size: 648 282
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 711 930
Size: 711 219
BseYI C'CCAG_C
Cuts at: 0 57 930
Size: 57 873
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 930
Size: 145 785
BsiHKAI G_wGCw'C
Cuts at: 0 421 930
Size: 421 509
BsmI GAATG_Cn'
Cuts at: 0 882 930
Size: 882 48
Bsp1286I G_dGCh'C
Cuts at: 0 140 421 897 930
Size: 140 281 476 33
Fragments arranged by size:
476 281 140 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 724 930
Size: 724 206
BsrBI CCG'CTC
Cuts at: 0 627 930
Size: 627 303
BsrGI T'GTAC_A
Cuts at: 0 770 930
Size: 770 160
BstYI r'GATC_y
Cuts at: 0 677 812 930
Size: 677 135 118
Fragments arranged by size:
677 135 118
Bsu36I CC'TnA_GG
Cuts at: 0 871 930
Size: 871 59
EaeI y'GGCC_r
Cuts at: 0 194 665 930
Size: 194 471 265
Fragments arranged by size:
471 265 194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 277 429 930
Size: 85 192 152 501
Fragments arranged by size:
501 192 152 85
HaeII r_GCGC'y
Cuts at: 0 761 930
Size: 761 169
MscI TGG'CCA
Cuts at: 0 196 667 930
Size: 196 471 263
Fragments arranged by size:
471 263 196
MslI CAynn'nnrTG
Cuts at: 0 169 616 661 766 772 930
Size: 169 447 45 105 6 158
Fragments arranged by size:
447 169 158 105 45 6
NheI G'CTAG_C
Cuts at: 0 240 930
Size: 240 690
NspI r_CATG'y
Cuts at: 0 210 930
Size: 210 720
PciI A'CATG_T
Cuts at: 0 206 930
Size: 206 724
PspXI vC'TCGA_Gb
Cuts at: 0 219 930
Size: 219 711
ScaI AGT'ACT
Cuts at: 0 225 930
Size: 225 705
SexAI A'CCwGG_T
Cuts at: 0 581 930
Size: 581 349
SfcI C'TryA_G
Cuts at: 0 99 864 930
Size: 99 765 66
Fragments arranged by size:
765 99 66
SmlI C'TyrA_G
Cuts at: 0 219 672 797 930
Size: 219 453 125 133
Fragments arranged by size:
453 219 133 125
StuI AGG'CCT
Cuts at: 0 706 930
Size: 706 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 183 930
Size: 183 747
TatI w'GTAC_w
Cuts at: 0 223 770 930
Size: 223 547 160
Fragments arranged by size:
547 223 160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 35 67 930
Size: 35 32 863
Fragments arranged by size:
863 35 32
XbaI T'CTAG_A
Cuts at: 0 154 930
Size: 154 776
XhoI C'TCGA_G
Cuts at: 0 219 930
Size: 219 711
Enzymes that do cut:
AcuI AflIII ApaLI AvaI BamHI BanII BbsI Bme1580I
BmtI BpmI BpuEI BsaI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrGI BstYI Bsu36I EaeI
Eco57MI HaeII MscI MslI NheI NspI PciI PspXI
ScaI SexAI SfcI SmlI StuI TaqII TatI TstI
XbaI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApoI AscI
AseI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI
BplI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmBI BspEI BspHI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EarI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StyI SwaI Tth111I XcmI XmaI
XmnI ZraI