Restriction map for
pFN21AE5915
(Linear) (Six-base) MAPSORT of: KIEE5915.seq Check: 4661 from: 1 to: 927
KIEE5915.orf
With 182 enzymes: SgfI *
December 5, 2014 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 721 927
Size: 721 206
AleI CACnn'nnGTG
Cuts at: 0 527 927
Size: 527 400
ApaLI G'TGCA_C
Cuts at: 0 417 927
Size: 417 510
BamHI G'GATC_C
Cuts at: 0 674 927
Size: 674 253
BanI G'GyrC_C
Cuts at: 0 889 927
Size: 889 38
BbeI G_GCGC'C
Cuts at: 0 893 927
Size: 893 34
BfrBI ATG'CAT
Cuts at: 0 287 927
Size: 287 640
Bme1580I G_kGCm'C
Cuts at: 0 421 906 927
Size: 421 485 21
Fragments arranged by size:
485 421 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 429 927
Size: 85 344 498
Fragments arranged by size:
498 344 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 690 927
Size: 532 158 237
Fragments arranged by size:
532 237 158
BsaI GGTCTCn'nnnn_
Cuts at: 0 645 927
Size: 645 282
BsaAI yAC'GTr
Cuts at: 0 324 767 927
Size: 324 443 160
Fragments arranged by size:
443 324 160
BsaHI Gr'CG_yC
Cuts at: 0 890 927
Size: 890 37
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 708 811 912 927
Size: 64 644 103 101 15
Fragments arranged by size:
644 103 101 64 15
BseYI C'CCAG_C
Cuts at: 0 114 927
Size: 114 813
BsiHKAI G_wGCw'C
Cuts at: 0 421 927
Size: 421 506
BsmI GAATG_Cn'
Cuts at: 0 879 927
Size: 879 48
Bsp1286I G_dGCh'C
Cuts at: 0 421 906 927
Size: 421 485 21
Fragments arranged by size:
485 421 21
BspHI T'CATG_A
Cuts at: 0 401 510 927
Size: 401 109 417
Fragments arranged by size:
417 401 109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 721 927
Size: 721 206
BstYI r'GATC_y
Cuts at: 0 674 927
Size: 674 253
Bsu36I CC'TnA_GG
Cuts at: 0 487 794 868 895 927
Size: 487 307 74 27 32
Fragments arranged by size:
487 307 74 32 27
EaeI y'GGCC_r
Cuts at: 0 194 659 927
Size: 194 465 268
Fragments arranged by size:
465 268 194
EarI CTCTTCn'nnn_
Cuts at: 0 748 927
Size: 748 179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 429 927
Size: 85 344 498
Fragments arranged by size:
498 344 85
FspI TGC'GCA
Cuts at: 0 652 927
Size: 652 275
HaeII r_GCGC'y
Cuts at: 0 758 893 927
Size: 758 135 34
Fragments arranged by size:
758 135 34
HincII GTy'rAC
Cuts at: 0 204 927
Size: 204 723
HindIII A'AGCT_T
Cuts at: 0 157 927
Size: 157 770
KasI G'GCGC_C
Cuts at: 0 889 927
Size: 889 38
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 927
Size: 245 682
MscI TGG'CCA
Cuts at: 0 196 927
Size: 196 731
MslI CAynn'nnrTG
Cuts at: 0 169 527 613 769 927
Size: 169 358 86 156 158
Fragments arranged by size:
358 169 158 156 86
NarI GG'CG_CC
Cuts at: 0 890 927
Size: 890 37
NsiI A_TGCA'T
Cuts at: 0 289 927
Size: 289 638
SexAI A'CCwGG_T
Cuts at: 0 578 927
Size: 578 349
SfoI GGC'GCC
Cuts at: 0 891 927
Size: 891 36
SmlI C'TyrA_G
Cuts at: 0 547 669 927
Size: 547 122 258
Fragments arranged by size:
547 258 122
StuI AGG'CCT
Cuts at: 0 703 927
Size: 703 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 183 432 927
Size: 183 249 495
Fragments arranged by size:
495 249 183
TatI w'GTAC_w
Cuts at: 0 173 927
Size: 173 754
Enzymes that do cut:
AarI AleI ApaLI BamHI BanI BbeI BfrBI Bme1580I
BpmI BpuEI BsaI BsaAI BsaHI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspHI BspMI BstYI Bsu36I EaeI EarI
Eco57MI FspI HaeII HincII HindIII KasI MmeI MscI
MslI NarI NsiI SexAI SfoI SmlI StuI TaqII
TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BsaBI BsaWI BsaXI BsgI BsiEI
BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspAI
Hin4I HpaI KpnI MfeI MluI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StyI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI