Restriction map for
pFN21AE5911
(Linear) (Six-base) MAPSORT of: KIEE5911.seq Check: 3762 from: 1 to: 930
KIEE5911.orf
With 182 enzymes: SgfI *
December 5, 2014 13:13 ..
AflIII A'CryG_T
Cuts at: 0 285 930
Size: 285 645
AleI CACnn'nnGTG
Cuts at: 0 17 930
Size: 17 913
AlwNI CAG_nnn'CTG
Cuts at: 0 407 682 930
Size: 407 275 248
Fragments arranged by size:
407 275 248
ApoI r'AATT_y
Cuts at: 0 28 501 930
Size: 28 473 429
Fragments arranged by size:
473 429 28
AseI AT'TA_AT
Cuts at: 0 112 930
Size: 112 818
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 387 420 930
Size: 387 33 510
Fragments arranged by size:
510 387 33
BbvCI CC'TCA_GC
Cuts at: 0 184 930
Size: 184 746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 930
Size: 223 707
Bpu10I CC'TnA_GC
Cuts at: 0 184 930
Size: 184 746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 930
Size: 649 281
BsaAI yAC'GTr
Cuts at: 0 765 930
Size: 765 165
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 637 667 930
Size: 637 30 263
Fragments arranged by size:
637 263 30
BstYI r'GATC_y
Cuts at: 0 206 668 930
Size: 206 462 262
Fragments arranged by size:
462 262 206
BtgI C'CryG_G
Cuts at: 0 314 930
Size: 314 616
EaeI y'GGCC_r
Cuts at: 0 684 930
Size: 684 246
EarI CTCTTCn'nnn_
Cuts at: 0 259 569 866 930
Size: 259 310 297 64
Fragments arranged by size:
310 297 259 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 930
Size: 223 707
EcoO109I rG'GnC_Cy
Cuts at: 0 53 885 930
Size: 53 832 45
Fragments arranged by size:
832 53 45
EcoRI G'AATT_C
Cuts at: 0 28 930
Size: 28 902
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 930
Size: 30 900
HincII GTy'rAC
Cuts at: 0 830 916 930
Size: 830 86 14
Fragments arranged by size:
830 86 14
HpaI GTT'AAC
Cuts at: 0 830 930
Size: 830 100
MfeI C'AATT_G
Cuts at: 0 535 930
Size: 535 395
MslI CAynn'nnrTG
Cuts at: 0 17 133 391 930
Size: 17 116 258 539
Fragments arranged by size:
539 258 116 17
MspA1I CmG'CkG
Cuts at: 0 749 930
Size: 749 181
NcoI C'CATG_G
Cuts at: 0 314 930
Size: 314 616
NspI r_CATG'y
Cuts at: 0 289 930
Size: 289 641
PasI CC'CwG_GG
Cuts at: 0 49 930
Size: 49 881
PciI A'CATG_T
Cuts at: 0 285 930
Size: 285 645
PpuMI rG'GwC_Cy
Cuts at: 0 53 930
Size: 53 877
PstI C_TGCA'G
Cuts at: 0 164 930
Size: 164 766
PvuII CAG'CTG
Cuts at: 0 749 930
Size: 749 181
SanDI GG'GwC_CC
Cuts at: 0 53 930
Size: 53 877
SapI GCTCTTCn'nnn_
Cuts at: 0 259 930
Size: 259 671
SfcI C'TryA_G
Cuts at: 0 160 930
Size: 160 770
SmlI C'TyrA_G
Cuts at: 0 664 930
Size: 664 266
StyI C'CwwG_G
Cuts at: 0 57 314 930
Size: 57 257 616
Fragments arranged by size:
616 257 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 405 423 542 930
Size: 405 18 119 388
Fragments arranged by size:
405 388 119 18
TatI w'GTAC_w
Cuts at: 0 794 930
Size: 794 136
Tth111I GACn'n_nGTC
Cuts at: 0 874 930
Size: 874 56
XmnI GAAnn'nnTTC
Cuts at: 0 697 847 930
Size: 697 150 83
Fragments arranged by size:
697 150 83
Enzymes that do cut:
AflIII AleI AlwNI ApoI AseI BaeI BbvCI BpmI
Bpu10I BpuEI BsaAI BsaXI BstYI BtgI EaeI EarI
Eco57MI EcoO109I EcoRI Hin4I HincII HpaI MfeI MslI
MspA1I NcoI NspI PasI PciI PpuMI PstI PvuII
SanDI SapI SfcI SmlI StyI TaqII TatI Tth111I
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BsaI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII
HindIII KasI KpnI MluI MmeI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI XbaI XcmI
XhoI XmaI ZraI