Restriction map for
pFN21AE5907
(Linear) (Six-base) MAPSORT of: KIEE5907.seq Check: 6165 from: 1 to: 930
KIEE5907.orf
With 182 enzymes: SgfI *
December 5, 2014 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 167 930
Size: 167 763
AleI CACnn'nnGTG
Cuts at: 0 112 930
Size: 112 818
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 256 288 930
Size: 256 32 642
Fragments arranged by size:
642 256 32
AseI AT'TA_AT
Cuts at: 0 859 930
Size: 859 71
AvaI C'yCGr_G
Cuts at: 0 799 930
Size: 799 131
BamHI G'GATC_C
Cuts at: 0 68 930
Size: 68 862
BanI G'GyrC_C
Cuts at: 0 436 930
Size: 436 494
BanII G_rGCy'C
Cuts at: 0 557 930
Size: 557 373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 648 930
Size: 648 282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 571 891 930
Size: 240 331 320 39
Fragments arranged by size:
331 320 240 39
BsaAI yAC'GTr
Cuts at: 0 366 930
Size: 366 564
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 213 243 930
Size: 213 30 687
Fragments arranged by size:
687 213 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 915 930
Size: 915 15
BseYI C'CCAG_C
Cuts at: 0 457 930
Size: 457 473
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 930
Size: 145 785
BsiHKAI G_wGCw'C
Cuts at: 0 557 930
Size: 557 373
BsmI GAATG_Cn'
Cuts at: 0 626 668 930
Size: 626 42 262
Fragments arranged by size:
626 262 42
BsmBI CGTCTCn'nnnn_
Cuts at: 0 327 930
Size: 327 603
Bsp1286I G_dGCh'C
Cuts at: 0 557 930
Size: 557 373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 167 686 930
Size: 167 519 244
Fragments arranged by size:
519 244 167
BsrGI T'GTAC_A
Cuts at: 0 389 930
Size: 389 541
BstEII G'GTnAC_C
Cuts at: 0 105 930
Size: 105 825
BstYI r'GATC_y
Cuts at: 0 68 930
Size: 68 862
Bsu36I CC'TnA_GG
Cuts at: 0 898 930
Size: 898 32
BtgI C'CryG_G
Cuts at: 0 110 464 930
Size: 110 354 466
Fragments arranged by size:
466 354 110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 361 930
Size: 361 569
DraIII CAC_nnn'GTG
Cuts at: 0 284 930
Size: 284 646
DrdI GACnn_nn'nnGTC
Cuts at: 0 514 930
Size: 514 416
EaeI y'GGCC_r
Cuts at: 0 368 930
Size: 368 562
EarI CTCTTCn'nnn_
Cuts at: 0 21 82 304 930
Size: 21 61 222 626
Fragments arranged by size:
626 222 61 21
EcoICRI GAG'CTC
Cuts at: 0 555 930
Size: 555 375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 648 930
Size: 648 282
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 53 85 930
Size: 53 32 845
Fragments arranged by size:
845 53 32
HincII GTy'rAC
Cuts at: 0 612 930
Size: 612 318
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 597 930
Size: 597 333
MscI TGG'CCA
Cuts at: 0 370 930
Size: 370 560
MslI CAynn'nnrTG
Cuts at: 0 112 169 930
Size: 112 57 761
Fragments arranged by size:
761 112 57
NcoI C'CATG_G
Cuts at: 0 464 930
Size: 464 466
PspXI vC'TCGA_Gb
Cuts at: 0 799 930
Size: 799 131
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 705 737 930
Size: 705 32 193
Fragments arranged by size:
705 193 32
SacI G_AGCT'C
Cuts at: 0 557 930
Size: 557 373
SapI GCTCTTCn'nnn_
Cuts at: 0 304 930
Size: 304 626
ScaI AGT'ACT
Cuts at: 0 714 930
Size: 714 216
SfcI C'TryA_G
Cuts at: 0 686 864 930
Size: 686 178 66
Fragments arranged by size:
686 178 66
SmlI C'TyrA_G
Cuts at: 0 255 550 799 870 930
Size: 255 295 249 71 60
Fragments arranged by size:
295 255 249 71 60
StyI C'CwwG_G
Cuts at: 0 464 930
Size: 464 466
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 424 930
Size: 424 506
TatI w'GTAC_w
Cuts at: 0 173 389 712 930
Size: 173 216 323 218
Fragments arranged by size:
323 218 216 173
XhoI C'TCGA_G
Cuts at: 0 799 930
Size: 799 131
Enzymes that do cut:
AarI AleI AloI AseI AvaI BamHI BanI BanII
BpmI BpuEI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrGI BstEII BstYI Bsu36I
BtgI BtgZI DraIII DrdI EaeI EarI EcoICRI Eco57MI
Hin4I HincII MmeI MscI MslI NcoI PspXI PsrI
SacI SapI ScaI SfcI SmlI StyI TaqII TatI
XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AscI
AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BsaI BsaBI BsaHI BsaWI BsiEI
BsiWI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstZ17I BtsI ClaI CspCI DraI
EagI EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI