(Linear) (Six-base) MAPSORT of: KIEE5906.seq  Check: 5789  from: 1  to: 930



KIEE5906.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:13  ..

AccI GT'mk_AC

Cuts at:      0     524     930 
   Size:        524     406
 
AflIII A'CryG_T

Cuts at:      0     212     930 
   Size:        212     718
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     406     930 
   Size:        406     524
 
ApaLI G'TGCA_C

Cuts at:      0     463     930 
   Size:        463     467
 
ApoI r'AATT_y

Cuts at:      0      31      72     495     930 
   Size:         31      41     423     435

  Fragments arranged by size: 

                 435     423      41      31
 
BclI T'GATC_A

Cuts at:      0     245     264     554     930 
   Size:        245      19     290     376

  Fragments arranged by size: 

                 376     290     245      19
 
Bme1580I G_kGCm'C

Cuts at:      0     467     930 
   Size:        467     463
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     645     930 
   Size:        645     285
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     159     631     658     930 
   Size:        159     472      27     272

  Fragments arranged by size: 

                 472     272     159      27
 
BseYI C'CCAG_C

Cuts at:      0     117     189     930 
   Size:        117      72     741

  Fragments arranged by size: 

                 741     117      72
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      36     148     710     930 
   Size:         36     112     562     220

  Fragments arranged by size: 

                 562     220     112      36
 
BsiHKAI G_wGCw'C

Cuts at:      0     344     467     930 
   Size:        344     123     463

  Fragments arranged by size: 

                 463     344     123
 
Bsp1286I G_dGCh'C

Cuts at:      0     344     467     930 
   Size:        344     123     463

  Fragments arranged by size: 

                 463     344     123
 
BsrFI r'CCGG_y

Cuts at:      0     691     930 
   Size:        691     239
 
BstEII G'GTnAC_C

Cuts at:      0     121     930 
   Size:        121     809
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     117     930 
   Size:        117     813
 
BtgI C'CryG_G

Cuts at:      0     353     930 
   Size:        353     577
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     580     930 
   Size:        580     350
 
EaeI y'GGCC_r

Cuts at:      0     107     374     930 
   Size:        107     267     556

  Fragments arranged by size: 

                 556     267     107
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     435     930 
   Size:        435     495
 
MscI TGG'CCA

Cuts at:      0     109     376     930 
   Size:        109     267     554

  Fragments arranged by size: 

                 554     267     109
 
MslI CAynn'nnrTG

Cuts at:      0     739     930 
   Size:        739     191
 
NcoI C'CATG_G

Cuts at:      0     353     930 
   Size:        353     577
 
NdeI CA'TA_TG

Cuts at:      0     379     930 
   Size:        379     551
 
NspI r_CATG'y

Cuts at:      0     216     930 
   Size:        216     714
 
PciI A'CATG_T

Cuts at:      0     212     930 
   Size:        212     718
 
PfoI T'CCnGG_A

Cuts at:      0     206     930 
   Size:        206     724
 
SmlI C'TyrA_G

Cuts at:      0     624     930 
   Size:        624     306
 
StyI C'CwwG_G

Cuts at:      0      20     353     930 
   Size:         20     333     577

  Fragments arranged by size: 

                 577     333      20
 
TatI w'GTAC_w

Cuts at:      0     179     930 
   Size:        179     751
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     477     930 
   Size:        477     453
 

 Enzymes that do cut:

     AccI   AflIII     AlfI    ApaLI     ApoI     BclI Bme1580I    BpuEI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrFI   BstEII    BstXI
     BtgI     DrdI     EaeI     MmeI     MscI     MslI     NcoI     NdeI
     NspI     PciI     PfoI     SmlI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
   MspA1I     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI