Restriction map for
pFN21AE5905
(Linear) (Six-base) MAPSORT of: KIEE5905.seq Check: 3722 from: 1 to: 927
KIEE5905.orf
With 182 enzymes: SgfI *
December 5, 2014 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 397 927
Size: 397 530
AccI GT'mk_AC
Cuts at: 0 200 927
Size: 200 727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 468 704 927
Size: 46 422 236 223
Fragments arranged by size:
422 236 223 46
AleI CACnn'nnGTG
Cuts at: 0 16 829 927
Size: 16 813 98
Fragments arranged by size:
813 98 16
AseI AT'TA_AT
Cuts at: 0 515 927
Size: 515 412
BanI G'GyrC_C
Cuts at: 0 478 927
Size: 478 449
BbsI GAAGACnn'nnnn_
Cuts at: 0 693 927
Size: 693 234
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 265 299 927
Size: 265 34 628
Fragments arranged by size:
628 265 34
BclI T'GATC_A
Cuts at: 0 134 927
Size: 134 793
BfrBI ATG'CAT
Cuts at: 0 656 675 927
Size: 656 19 252
Fragments arranged by size:
656 252 19
BglI GCCn_nnn'nGGC
Cuts at: 0 346 927
Size: 346 581
Bme1580I G_kGCm'C
Cuts at: 0 420 927
Size: 420 507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 765 927
Size: 765 162
BsaBI GATnn'nnATC
Cuts at: 0 133 927
Size: 133 794
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 59 89 133 163 927
Size: 59 30 44 30 764
Fragments arranged by size:
764 59 44 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 927
Size: 64 863
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 927
Size: 305 622
BsiHKAI G_wGCw'C
Cuts at: 0 891 927
Size: 891 36
BsmI GAATG_Cn'
Cuts at: 0 675 927
Size: 675 252
Bsp1286I G_dGCh'C
Cuts at: 0 420 891 927
Size: 420 471 36
Fragments arranged by size:
471 420 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 397 927
Size: 397 530
BsrDI GCAATG_nn'
Cuts at: 0 244 927
Size: 244 683
BstXI CCAn_nnnn'nTGG
Cuts at: 0 237 927
Size: 237 690
Bsu36I CC'TnA_GG
Cuts at: 0 865 927
Size: 865 62
BtgI C'CryG_G
Cuts at: 0 344 791 927
Size: 344 447 136
Fragments arranged by size:
447 344 136
BtsI GCAGTG_nn'
Cuts at: 0 725 927
Size: 725 202
EarI CTCTTCn'nnn_
Cuts at: 0 386 927
Size: 386 541
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 468 704 765 927
Size: 46 422 236 61 162
Fragments arranged by size:
422 236 162 61 46
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 717 927
Size: 717 210
MslI CAynn'nnrTG
Cuts at: 0 16 166 397 760 829 927
Size: 16 150 231 363 69 98
Fragments arranged by size:
363 231 150 98 69 16
MspA1I CmG'CkG
Cuts at: 0 220 367 927
Size: 220 147 560
Fragments arranged by size:
560 220 147
NcoI C'CATG_G
Cuts at: 0 344 927
Size: 344 583
NdeI CA'TA_TG
Cuts at: 0 652 927
Size: 652 275
NsiI A_TGCA'T
Cuts at: 0 658 677 927
Size: 658 19 250
Fragments arranged by size:
658 250 19
PflMI CCAn_nnn'nTGG
Cuts at: 0 792 927
Size: 792 135
PvuII CAG'CTG
Cuts at: 0 220 927
Size: 220 707
SfcI C'TryA_G
Cuts at: 0 221 927
Size: 221 706
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 346 927
Size: 346 581
StuI AGG'CCT
Cuts at: 0 378 927
Size: 378 549
StyI C'CwwG_G
Cuts at: 0 344 927
Size: 344 583
TatI w'GTAC_w
Cuts at: 0 170 764 927
Size: 170 594 163
Fragments arranged by size:
594 170 163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 116 133 148 165 927
Size: 116 17 15 17 762
Fragments arranged by size:
762 116 17 17 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 789 927
Size: 789 138
XmnI GAAnn'nnTTC
Cuts at: 0 700 927
Size: 700 227
Enzymes that do cut:
AarI AccI AcuI AleI AseI BanI BbsI BcgI
BclI BfrBI BglI Bme1580I BpmI BsaBI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BstXI Bsu36I
BtgI BtsI EarI Eco57MI FalI MslI MspA1I NcoI
NdeI NsiI PflMI PvuII SfcI SfiI StuI StyI
TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbvCI BciVI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BseYI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NgoMIV NheI NotI NruI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI