Restriction map for
pFN21AE5903
(Linear) (Six-base) MAPSORT of: KIEE5903.seq Check: 4699 from: 1 to: 927
KIEE5903.orf
With 182 enzymes: SgfI *
December 5, 2014 13:13 ..
AleI CACnn'nnGTG
Cuts at: 0 109 927
Size: 109 818
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 927
Size: 397 530
BbsI GAAGACnn'nnnn_
Cuts at: 0 708 927
Size: 708 219
BclI T'GATC_A
Cuts at: 0 236 927
Size: 236 691
BfrBI ATG'CAT
Cuts at: 0 284 927
Size: 284 643
Bme1580I G_kGCm'C
Cuts at: 0 420 752 927
Size: 420 332 175
Fragments arranged by size:
420 332 175
BmrI ACTGGGnnnn_n'
Cuts at: 0 166 927
Size: 166 761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 879 927
Size: 275 604 48
Fragments arranged by size:
604 275 48
BsaI GGTCTCn'nnnn_
Cuts at: 0 645 765 927
Size: 645 120 162
Fragments arranged by size:
645 162 120
BseYI C'CCAG_C
Cuts at: 0 36 927
Size: 36 891
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 543 927
Size: 341 202 384
Fragments arranged by size:
384 341 202
BsiHKAI G_wGCw'C
Cuts at: 0 125 664 927
Size: 125 539 263
Fragments arranged by size:
539 263 125
BsmI GAATG_Cn'
Cuts at: 0 870 927
Size: 870 57
Bsp1286I G_dGCh'C
Cuts at: 0 125 420 664 752 927
Size: 125 295 244 88 175
Fragments arranged by size:
295 244 175 125 88
BspHI T'CATG_A
Cuts at: 0 398 927
Size: 398 529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 783 927
Size: 783 144
BsrDI GCAATG_nn'
Cuts at: 0 587 927
Size: 587 340
BsrGI T'GTAC_A
Cuts at: 0 764 927
Size: 764 163
BstEII G'GTnAC_C
Cuts at: 0 132 927
Size: 132 795
BstYI r'GATC_y
Cuts at: 0 470 927
Size: 470 457
Bsu36I CC'TnA_GG
Cuts at: 0 667 927
Size: 667 260
BtsI GCAGTG_nn'
Cuts at: 0 140 927
Size: 140 787
DraIII CAC_nnn'GTG
Cuts at: 0 363 927
Size: 363 564
EaeI y'GGCC_r
Cuts at: 0 365 927
Size: 365 562
EarI CTCTTCn'nnn_
Cuts at: 0 481 589 927
Size: 481 108 338
Fragments arranged by size:
481 338 108
EcoO109I rG'GnC_Cy
Cuts at: 0 161 927
Size: 161 766
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 107 139 927
Size: 107 32 788
Fragments arranged by size:
788 107 32
MscI TGG'CCA
Cuts at: 0 367 927
Size: 367 560
MslI CAynn'nnrTG
Cuts at: 0 106 109 571 760 927
Size: 106 3 462 189 167
Fragments arranged by size:
462 189 167 106 3
NdeI CA'TA_TG
Cuts at: 0 572 927
Size: 572 355
NsiI A_TGCA'T
Cuts at: 0 286 927
Size: 286 641
PflMI CCAn_nnn'nTGG
Cuts at: 0 363 576 927
Size: 363 213 351
Fragments arranged by size:
363 351 213
PpuMI rG'GwC_Cy
Cuts at: 0 161 927
Size: 161 766
PshAI GACnn'nnGTC
Cuts at: 0 292 927
Size: 292 635
PstI C_TGCA'G
Cuts at: 0 780 927
Size: 780 147
SanDI GG'GwC_CC
Cuts at: 0 161 927
Size: 161 766
SfcI C'TryA_G
Cuts at: 0 776 927
Size: 776 151
SmlI C'TyrA_G
Cuts at: 0 290 858 927
Size: 290 568 69
Fragments arranged by size:
568 290 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 583 927
Size: 583 344
TatI w'GTAC_w
Cuts at: 0 764 927
Size: 764 163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 153 927
Size: 153 774
Enzymes that do cut:
AleI AlfI BbsI BclI BfrBI Bme1580I BmrI BpuEI
BsaI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrGI BstEII BstYI Bsu36I BtsI DraIII EaeI
EarI EcoO109I Hin4I MscI MslI NdeI NsiI PflMI
PpuMI PshAI PstI SanDI SfcI SmlI TaqII TatI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BglI BglII BlpI
BmgBI BmtI BplI BpmI Bpu10I BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BsiEI BsiWI BsmBI BspEI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgI
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI
Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI