(Linear) (Six-base) MAPSORT of: KIEE5902.seq  Check: 2342  from: 1  to: 927



KIEE5902.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:13  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     927 
   Size:         41     886
 
AflIII A'CryG_T

Cuts at:      0     487     718     770     927 
   Size:        487     231      52     157

  Fragments arranged by size: 

                 487     231     157      52
 
AlwNI CAG_nnn'CTG

Cuts at:      0     332     927 
   Size:        332     595
 
ApoI r'AATT_y

Cuts at:      0      31     267     927 
   Size:         31     236     660

  Fragments arranged by size: 

                 660     236      31
 
BamHI G'GATC_C

Cuts at:      0     360     927 
   Size:        360     567
 
BanI G'GyrC_C

Cuts at:      0      52     927 
   Size:         52     875
 
BanII G_rGCy'C

Cuts at:      0     457     927 
   Size:        457     470
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     677     927 
   Size:        677     250
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     401     435     927 
   Size:        401      34     492

  Fragments arranged by size: 

                 492     401      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      85     927 
   Size:         85     842
 
Bme1580I G_kGCm'C

Cuts at:      0      57     927 
   Size:         57     870
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     638     927 
   Size:        638     289
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     894     927 
   Size:        894      33
 
BsaAI yAC'GTr

Cuts at:      0     176     927 
   Size:        176     751
 
BseYI C'CCAG_C

Cuts at:      0      56     927 
   Size:         56     871
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     713     927 
   Size:        713     214
 
Bsp1286I G_dGCh'C

Cuts at:      0      57     457     927 
   Size:         57     400     470

  Fragments arranged by size: 

                 470     400      57
 
BsrGI T'GTAC_A

Cuts at:      0     471     927 
   Size:        471     456
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     319     927 
   Size:        319     608
 
BstYI r'GATC_y

Cuts at:      0     360     610     927 
   Size:        360     250     317

  Fragments arranged by size: 

                 360     317     250
 
BtsI GCAGTG_nn'

Cuts at:      0     362     927 
   Size:        362     565
 
DraI TTT'AAA

Cuts at:      0     902     927 
   Size:        902      25
 
EaeI y'GGCC_r

Cuts at:      0     665     927 
   Size:        665     262
 
EarI CTCTTCn'nnn_

Cuts at:      0      85     927 
   Size:         85     842
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     927 
   Size:         41     886
 
EcoRI G'AATT_C

Cuts at:      0      31     927 
   Size:         31     896
 
EcoRV GAT'ATC

Cuts at:      0     210     927 
   Size:        210     717
 
HincII GTy'rAC

Cuts at:      0     576     927 
   Size:        576     351
 
HindIII A'AGCT_T

Cuts at:      0     249     896     927 
   Size:        249     647      31

  Fragments arranged by size: 

                 647     249      31
 
MscI TGG'CCA

Cuts at:      0     667     927 
   Size:        667     260
 
MslI CAynn'nnrTG

Cuts at:      0     103     927 
   Size:        103     824
 
NspI r_CATG'y

Cuts at:      0     491     722     774     927 
   Size:        491     231      52     153

  Fragments arranged by size: 

                 491     231     153      52
 
PciI A'CATG_T

Cuts at:      0     487     718     770     927 
   Size:        487     231      52     157

  Fragments arranged by size: 

                 487     231     157      52
 
PshAI GACnn'nnGTC

Cuts at:      0     739     927 
   Size:        739     188
 
SfcI C'TryA_G

Cuts at:      0     867     927 
   Size:        867      60
 
SmlI C'TyrA_G

Cuts at:      0     873     927 
   Size:        873      54
 
TatI w'GTAC_w

Cuts at:      0     471     927 
   Size:        471     456
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     828     860     927 
   Size:        828      32      67

  Fragments arranged by size: 

                 828      67      32
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI    BamHI     BanI    BanII     BbsI
     BcgI    BciVI Bme1580I     BmrI    BpuEI    BsaAI    BseYI     BsgI
 Bsp1286I    BsrGI   BstAPI    BstYI     BtsI     DraI     EaeI     EarI
  Eco57MI    EcoRI    EcoRV   HincII  HindIII     MscI     MslI     NspI
     PciI    PshAI     SfcI     SmlI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BbeI    BbvCI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI EcoO109I     FalI     FseI     FspI    FspAI    HaeII
    Hin4I     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI