(Linear) (Six-base) MAPSORT of: KIEE5840.seq Check: 1511 from: 1 to: 7851 KIEE5840.orf With 182 enzymes: SgfI * December 5, 2014 13:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 3181 4030 4750 5352 7851 Size: 3181 849 720 602 2499 Fragments arranged by size: 3181 2499 849 720 602 AatII G_ACGT'C Cuts at: 0 3402 7851 Size: 3402 4449 AccI GT'mk_AC Cuts at: 0 3413 5736 7851 Size: 3413 2323 2115 Fragments arranged by size: 3413 2323 2115 Acc65I G'GTAC_C Cuts at: 0 5389 6438 7851 Size: 5389 1049 1413 Fragments arranged by size: 5389 1413 1049 AclI AA'CG_TT Cuts at: 0 5461 7045 7851 Size: 5461 1584 806 Fragments arranged by size: 5461 1584 806 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1574 1814 2366 2836 3113 6524 6621 6795 Size: 1574 240 552 470 277 3411 97 174 Cuts at: 6795 7568 7838 7851 Size: 773 270 13 Fragments arranged by size: 3411 1574 773 552 470 277 270 240 174 97 13 AflII C'TTAA_G Cuts at: 0 2401 7851 Size: 2401 5450 AflIII A'CryG_T Cuts at: 0 1248 1347 2148 3603 7851 Size: 1248 99 801 1455 4248 Fragments arranged by size: 4248 1455 1248 801 99 AhdI GACnn_n'nnGTC Cuts at: 0 297 2598 7851 Size: 297 2301 5253 Fragments arranged by size: 5253 2301 297 AleI CACnn'nnGTG Cuts at: 0 2101 4576 7851 Size: 2101 2475 3275 Fragments arranged by size: 3275 2475 2101 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2183 4058 4361 5903 6165 7851 Size: 2183 1875 303 1542 262 1686 Fragments arranged by size: 2183 1875 1686 1542 303 262 AlwNI CAG_nnn'CTG Cuts at: 0 505 1585 1774 2922 3796 4045 5773 5838 Size: 505 1080 189 1148 874 249 1728 65 Cuts at: 5838 6304 6958 7507 7591 7851 Size: 466 654 549 84 260 Fragments arranged by size: 1728 1148 1080 874 654 549 505 466 260 249 189 84 65 ApaLI G'TGCA_C Cuts at: 0 542 1842 7851 Size: 542 1300 6009 Fragments arranged by size: 6009 1300 542 ApoI r'AATT_y Cuts at: 0 1108 2838 3916 4443 4842 4860 7124 7539 Size: 1108 1730 1078 527 399 18 2264 415 Cuts at: 7539 7851 Size: 312 Fragments arranged by size: 2264 1730 1108 1078 527 415 399 312 18 AseI AT'TA_AT Cuts at: 0 3542 3647 7851 Size: 3542 105 4204 Fragments arranged by size: 4204 3542 105 AvaI C'yCGr_G Cuts at: 0 1436 3428 6174 6392 7769 7851 Size: 1436 1992 2746 218 1377 82 Fragments arranged by size: 2746 1992 1436 1377 218 82 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 6108 6141 7851 Size: 6108 33 1710 Fragments arranged by size: 6108 1710 33 BamHI G'GATC_C Cuts at: 0 119 414 6462 7851 Size: 119 295 6048 1389 Fragments arranged by size: 6048 1389 295 119 BanI G'GyrC_C Cuts at: 0 25 271 1870 2142 5176 5389 6438 6663 Size: 25 246 1599 272 3034 213 1049 225 Cuts at: 6663 6801 7429 7679 7851 Size: 138 628 250 172 Fragments arranged by size: 3034 1599 1049 628 272 250 246 225 213 172 138 25 BanII G_rGCy'C Cuts at: 0 3948 6183 7851 Size: 3948 2235 1668 Fragments arranged by size: 3948 2235 1668 BbsI GAAGACnn'nnnn_ Cuts at: 0 2625 5318 7851 Size: 2625 2693 2533 Fragments arranged by size: 2693 2625 2533 BbvCI CC'TCA_GC Cuts at: 0 250 7451 7619 7851 Size: 250 7201 168 232 Fragments arranged by size: 7201 250 232 168 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 5581 5615 7851 Size: 5581 34 2236 Fragments arranged by size: 5581 2236 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1405 3134 3360 7851 Size: 1405 1729 226 4491 Fragments arranged by size: 4491 1729 1405 226 BclI T'GATC_A Cuts at: 0 1902 2913 5225 7851 Size: 1902 1011 2312 2626 Fragments arranged by size: 2626 2312 1902 1011 BfrBI ATG'CAT Cuts at: 0 780 934 4169 5416 7275 7851 Size: 780 154 3235 1247 1859 576 Fragments arranged by size: 3235 1859 1247 780 576 154 BglI GCCn_nnn'nGGC Cuts at: 0 3987 5575 7567 7851 Size: 3987 1588 1992 284 Fragments arranged by size: 3987 1992 1588 284 BglII A'GATC_T Cuts at: 0 333 7851 Size: 333 7518 Bme1580I G_kGCm'C Cuts at: 0 546 1846 3023 5181 7851 Size: 546 1300 1177 2158 2670 Fragments arranged by size: 2670 2158 1300 1177 546 BmrI ACTGGGnnnn_n' Cuts at: 0 340 894 2374 2770 3563 5524 5619 5668 Size: 340 554 1480 396 793 1961 95 49 Cuts at: 5668 6462 7851 Size: 794 1389 Fragments arranged by size: 1961 1480 1389 794 793 554 396 340 95 49 BmtI G_CTAG'C Cuts at: 0 1333 7851 Size: 1333 6518 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 6078 7851 Size: 6078 1773 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 962 2257 2431 2945 3512 3818 5402 5897 Size: 962 1295 174 514 567 306 1584 495 Cuts at: 5897 6167 7733 7829 7851 Size: 270 1566 96 22 Fragments arranged by size: 1584 1566 1295 962 567 514 495 306 270 174 96 22 Bpu10I CC'TnA_GC Cuts at: 0 250 5300 5544 7451 7619 7851 Size: 250 5050 244 1907 168 232 Fragments arranged by size: 5050 1907 250 244 232 168 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1271 1496 1978 2111 3855 6011 6494 7851 Size: 1271 225 482 133 1744 2156 483 1357 Fragments arranged by size: 2156 1744 1357 1271 483 482 225 133 BsaI GGTCTCn'nnnn_ Cuts at: 0 3655 5673 7851 Size: 3655 2018 2178 Fragments arranged by size: 3655 2178 2018 BsaAI yAC'GTr Cuts at: 0 2149 7851 Size: 2149 5702 BsaBI GATnn'nnATC Cuts at: 0 4546 7851 Size: 4546 3305 BsaHI Gr'CG_yC Cuts at: 0 3399 7851 Size: 3399 4452 BsaWI w'CCGG_w Cuts at: 0 515 4067 7851 Size: 515 3552 3784 Fragments arranged by size: 3784 3552 515 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 935 965 1334 1364 3485 3515 3830 3860 Size: 935 30 369 30 2121 30 315 30 Cuts at: 3860 5844 5874 5895 5925 7127 7157 7768 7798 Size: 1984 30 21 30 1202 30 611 30 Cuts at: 7798 7851 Size: 53 Fragments arranged by size: 2121 1984 1202 935 611 369 315 53 30 30 30 30 30 30 30 30 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 52 2353 2642 4489 5153 5958 6250 6890 Size: 52 2301 289 1847 664 805 292 640 Cuts at: 6890 7773 7826 7851 Size: 883 53 25 Fragments arranged by size: 2301 1847 883 805 664 640 292 289 53 52 25 BseYI C'CCAG_C Cuts at: 0 72 76 3529 4615 6582 6615 6680 6824 Size: 72 4 3453 1086 1967 33 65 144 Cuts at: 6824 7562 7851 Size: 738 289 Fragments arranged by size: 3453 1967 1086 738 289 144 72 65 33 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 548 1061 1103 1205 1870 1949 3209 4042 Size: 548 513 42 102 665 79 1260 833 Cuts at: 4042 4778 5957 6550 7851 Size: 736 1179 593 1301 Fragments arranged by size: 1301 1260 1179 833 736 665 593 548 513 102 79 42 BsiEI CG_ry'CG Cuts at: 0 7654 7699 7851 Size: 7654 45 152 Fragments arranged by size: 7654 152 45 BsiHKAI G_wGCw'C Cuts at: 0 546 971 1125 1199 1287 1846 2620 3221 Size: 546 425 154 74 88 559 774 601 Cuts at: 3221 5433 6183 7851 Size: 2212 750 1668 Fragments arranged by size: 2212 1668 774 750 601 559 546 425 154 88 74 BsmI GAATG_Cn' Cuts at: 0 4171 4284 6413 7555 7851 Size: 4171 113 2129 1142 296 Fragments arranged by size: 4171 2129 1142 296 113 Bsp1286I G_dGCh'C Cuts at: 0 546 971 1125 1199 1287 1846 2620 3023 Size: 546 425 154 74 88 559 774 403 Cuts at: 3023 3221 3948 5181 5433 6183 7851 Size: 198 727 1233 252 750 1668 Fragments arranged by size: 1668 1233 774 750 727 559 546 425 403 252 198 154 88 74 BspEI T'CCGG_A Cuts at: 0 515 7851 Size: 515 7336 BspHI T'CATG_A Cuts at: 0 879 3162 3198 7851 Size: 879 2283 36 4653 Fragments arranged by size: 4653 2283 879 36 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1033 3181 4030 4750 5352 6021 6312 7851 Size: 1033 2148 849 720 602 669 291 1539 Fragments arranged by size: 2148 1539 1033 849 720 669 602 291 BsrDI GCAATG_nn' Cuts at: 0 442 3099 3221 3599 4993 7731 7851 Size: 442 2657 122 378 1394 2738 120 Fragments arranged by size: 2738 2657 1394 442 378 122 120 BssSI C'ACGA_G Cuts at: 0 6704 7851 Size: 6704 1147 BstAPI GCAn_nnn'nTGC Cuts at: 0 1039 3232 4561 4799 7851 Size: 1039 2193 1329 238 3052 Fragments arranged by size: 3052 2193 1329 1039 238 BstEII G'GTnAC_C Cuts at: 0 6851 7851 Size: 6851 1000 BstYI r'GATC_y Cuts at: 0 119 333 414 501 5292 6462 7503 7851 Size: 119 214 81 87 4791 1170 1041 348 Fragments arranged by size: 4791 1170 1041 348 214 119 87 81 Bsu36I CC'TnA_GG Cuts at: 0 506 2690 4436 7851 Size: 506 2184 1746 3415 Fragments arranged by size: 3415 2184 1746 506 BtgI C'CryG_G Cuts at: 0 2171 5618 6110 6434 7851 Size: 2171 3447 492 324 1417 Fragments arranged by size: 3447 2171 1417 492 324 BtsI GCAGTG_nn' Cuts at: 0 288 917 1217 4824 4879 5153 6533 7851 Size: 288 629 300 3607 55 274 1380 1318 Fragments arranged by size: 3607 1380 1318 629 300 288 274 55 ClaI AT'CG_AT Cuts at: 0 3109 7851 Size: 3109 4742 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 3077 3112 4524 4559 7028 7063 7851 Size: 3077 35 1412 35 2469 35 788 Fragments arranged by size: 3077 2469 1412 788 35 35 35 DraI TTT'AAA Cuts at: 0 2712 4295 7851 Size: 2712 1583 3556 Fragments arranged by size: 3556 2712 1583 DraIII CAC_nnn'GTG Cuts at: 0 3056 7851 Size: 3056 4795 EaeI y'GGCC_r Cuts at: 0 2034 7651 7851 Size: 2034 5617 200 Fragments arranged by size: 5617 2034 200 EagI C'GGCC_G Cuts at: 0 7651 7851 Size: 7651 200 EarI CTCTTCn'nnn_ Cuts at: 0 2468 3813 4319 4466 5130 5817 6897 7633 Size: 2468 1345 506 147 664 687 1080 736 Cuts at: 7633 7833 7851 Size: 200 18 Fragments arranged by size: 2468 1345 1080 736 687 664 506 200 147 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 57 86 221 7851 Size: 57 29 135 7630 Fragments arranged by size: 7630 135 57 29 EcoICRI GAG'CTC Cuts at: 0 6181 7851 Size: 6181 1670 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 962 1574 1814 2257 2366 2431 2836 2945 Size: 962 612 240 443 109 65 405 109 Cuts at: 2945 3113 3512 3818 5402 5897 6167 6524 6621 Size: 168 399 306 1584 495 270 357 97 Cuts at: 6621 6795 7568 7733 7829 7838 7851 Size: 174 773 165 96 9 13 Fragments arranged by size: 1584 962 773 612 495 443 405 399 357 306 270 240 174 168 165 109 109 97 96 65 13 9 EcoO109I rG'GnC_Cy Cuts at: 0 42 80 2289 7474 7851 Size: 42 38 2209 5185 377 Fragments arranged by size: 5185 2209 377 42 38 EcoRI G'AATT_C Cuts at: 0 1108 4443 4860 7124 7851 Size: 1108 3335 417 2264 727 Fragments arranged by size: 3335 2264 1108 727 417 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 787 988 1826 2416 6052 7851 Size: 787 201 838 590 3636 1799 Fragments arranged by size: 3636 1799 838 787 590 201 HaeII r_GCGC'y Cuts at: 0 223 1676 7016 7851 Size: 223 1453 5340 835 Fragments arranged by size: 5340 1453 835 223 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 34 66 283 295 315 327 880 912 Size: 34 32 217 12 20 12 553 32 Cuts at: 912 2584 2616 2725 2757 2826 2858 5135 5167 Size: 1672 32 109 32 69 32 2277 32 Cuts at: 5167 5589 5621 5895 5927 6076 6108 6454 6486 Size: 422 32 274 32 149 32 346 32 Cuts at: 6486 7851 Size: 1365 Fragments arranged by size: 2277 1672 1365 553 422 346 274 217 149 109 69 34 32 32 32 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 0 476 894 954 1881 2697 3135 4973 7851 Size: 476 418 60 927 816 438 1838 2878 Fragments arranged by size: 2878 1838 927 816 476 438 418 60 HindIII A'AGCT_T Cuts at: 0 491 632 7851 Size: 491 141 7219 Fragments arranged by size: 7219 491 141 HpaI GTT'AAC Cuts at: 0 1881 7851 Size: 1881 5970 KpnI G_GTAC'C Cuts at: 0 5393 6442 7851 Size: 5393 1049 1409 Fragments arranged by size: 5393 1409 1049 MfeI C'AATT_G Cuts at: 0 7100 7851 Size: 7100 751 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2042 3056 3282 3795 5458 5530 7164 7811 Size: 2042 1014 226 513 1663 72 1634 647 Cuts at: 7811 7851 Size: 40 Fragments arranged by size: 2042 1663 1634 1014 647 513 226 72 40 MscI TGG'CCA Cuts at: 0 2036 7851 Size: 2036 5815 MslI CAynn'nnrTG Cuts at: 0 785 1103 1742 2042 2101 3384 3953 4013 Size: 785 318 639 300 59 1283 569 60 Cuts at: 4013 4223 4576 5186 7851 Size: 210 353 610 2665 Fragments arranged by size: 2665 1283 785 639 610 569 353 318 300 210 60 59 MspA1I CmG'CkG Cuts at: 0 76 176 191 197 3004 6523 6544 7851 Size: 76 100 15 6 2807 3519 21 1307 Fragments arranged by size: 3519 2807 1307 100 76 21 15 6 NcoI C'CATG_G Cuts at: 0 2171 5618 6110 6434 7851 Size: 2171 3447 492 324 1417 Fragments arranged by size: 3447 2171 1417 492 324 NdeI CA'TA_TG Cuts at: 0 4694 7851 Size: 4694 3157 NheI G'CTAG_C Cuts at: 0 1329 7851 Size: 1329 6522 NsiI A_TGCA'T Cuts at: 0 782 936 4171 5418 7277 7851 Size: 782 154 3235 1247 1859 574 Fragments arranged by size: 3235 1859 1247 782 574 154 NspI r_CATG'y Cuts at: 0 784 938 1252 1299 1351 1687 1928 3301 Size: 784 154 314 47 52 336 241 1373 Cuts at: 3301 3389 3607 6783 7851 Size: 88 218 3176 1068 Fragments arranged by size: 3176 1373 1068 784 336 314 241 218 154 88 52 47 PciI A'CATG_T Cuts at: 0 1248 1347 3603 7851 Size: 1248 99 2256 4248 Fragments arranged by size: 4248 2256 1248 99 PflMI CCAn_nnn'nTGG Cuts at: 0 2322 6111 7851 Size: 2322 3789 1740 Fragments arranged by size: 3789 2322 1740 PfoI T'CCnGG_A Cuts at: 0 7467 7851 Size: 7467 384 PmlI CAC'GTG Cuts at: 0 2149 7851 Size: 2149 5702 PpuMI rG'GwC_Cy Cuts at: 0 42 2289 7851 Size: 42 2247 5562 Fragments arranged by size: 5562 2247 42 PshAI GACnn'nnGTC Cuts at: 0 3905 7851 Size: 3905 3946 PspXI vC'TCGA_Gb Cuts at: 0 3428 6392 7851 Size: 3428 2964 1459 Fragments arranged by size: 3428 2964 1459 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 3351 3383 4103 4135 7851 Size: 3351 32 720 32 3716 Fragments arranged by size: 3716 3351 720 32 32 PstI C_TGCA'G Cuts at: 0 390 1305 2516 3615 4266 6528 7851 Size: 390 915 1211 1099 651 2262 1323 Fragments arranged by size: 2262 1323 1211 1099 915 651 390 PvuII CAG'CTG Cuts at: 0 76 3004 6523 6544 7851 Size: 76 2928 3519 21 1307 Fragments arranged by size: 3519 2928 1307 76 21 SacI G_AGCT'C Cuts at: 0 6183 7851 Size: 6183 1668 SapI GCTCTTCn'nnn_ Cuts at: 0 2468 7851 Size: 2468 5383 ScaI AGT'ACT Cuts at: 0 384 4914 6021 7851 Size: 384 4530 1107 1830 Fragments arranged by size: 4530 1830 1107 384 SfcI C'TryA_G Cuts at: 0 386 1301 1889 2512 3611 4262 4664 5816 Size: 386 915 588 623 1099 651 402 1152 Cuts at: 5816 6524 6713 7851 Size: 708 189 1138 Fragments arranged by size: 1152 1138 1099 915 708 651 623 588 402 386 189 SmaI CCC'GGG Cuts at: 0 1438 6176 7771 7851 Size: 1438 4738 1595 80 Fragments arranged by size: 4738 1595 1438 80 SmlI C'TyrA_G Cuts at: 0 1286 1511 1993 2126 2401 3428 3870 6026 Size: 1286 225 482 133 275 1027 442 2156 Cuts at: 6026 6392 6509 7851 Size: 366 117 1342 Fragments arranged by size: 2156 1342 1286 1027 482 442 366 275 225 133 117 SpeI A'CTAG_T Cuts at: 0 6838 7851 Size: 6838 1013 SphI G_CATG'C Cuts at: 0 784 1687 7851 Size: 784 903 6164 Fragments arranged by size: 6164 903 784 SspI AAT'ATT Cuts at: 0 1657 2501 4126 7851 Size: 1657 844 1625 3725 Fragments arranged by size: 3725 1657 1625 844 StuI AGG'CCT Cuts at: 0 1234 7851 Size: 1234 6617 StyI C'CwwG_G Cuts at: 0 2171 5618 6110 6434 7003 7851 Size: 2171 3447 492 324 569 848 Fragments arranged by size: 3447 2171 848 569 492 324 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 100 2324 3749 5416 6090 6209 6342 7647 Size: 100 2224 1425 1667 674 119 133 1305 Cuts at: 7647 7713 7744 7851 Size: 66 31 107 Fragments arranged by size: 2224 1667 1425 1305 674 133 119 107 100 66 31 TatI w'GTAC_w Cuts at: 0 382 4912 6019 7851 Size: 382 4530 1107 1832 Fragments arranged by size: 4530 1832 1107 382 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 246 278 399 431 3260 3292 7768 7800 Size: 246 32 121 32 2829 32 4476 32 Cuts at: 7800 7851 Size: 51 Fragments arranged by size: 4476 2829 246 121 51 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 4989 7851 Size: 4989 2862 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1614 2568 7508 7851 Size: 1614 954 4940 343 Fragments arranged by size: 4940 1614 954 343 XhoI C'TCGA_G Cuts at: 0 3428 6392 7851 Size: 3428 2964 1459 Fragments arranged by size: 3428 2964 1459 XmaI C'CCGG_G Cuts at: 0 1436 6174 7769 7851 Size: 1436 4738 1595 82 Fragments arranged by size: 4738 1595 1436 82 ZraI GAC'GTC Cuts at: 0 3400 7851 Size: 3400 4451 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AhdI AleI AlfI AlwNI ApaLI ApoI AseI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BssSI BstAPI BstEII BstYI Bsu36I BtgI BtsI ClaI CspCI DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MmeI MscI MslI MspA1I NcoI NdeI NheI NsiI NspI PciI PflMI PfoI PmlI PpuMI PshAI PspXI PsrI PstI PvuII SacI SapI ScaI SfcI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI ZraI Enzymes that do not cut: AfeI AgeI AloI ApaI AscI AsiSI AvrII BbeI BlpI BmgBI BsiWI BsmBI BsrBI BsrFI BsrGI BssHII BstBI BstXI BstZ17I BtgZI DrdI EcoNI EcoRV FseI FspI FspAI KasI MluI NaeI NarI NgoMIV NotI NruI PacI PasI PmeI PpiI PsiI PspOMI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SrfI SwaI XbaI XmnI