Restriction map for
pFN21AE5830
(Linear) (Six-base) MAPSORT of: KIEE5830.seq Check: 119 from: 1 to: 3177
KIEE5830.orf
With 182 enzymes: SgfI *
December 5, 2014 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1424 1592 1876 3177
Size: 1424 168 284 1301
Fragments arranged by size:
1424 1301 284 168
AfeI AGC'GCT
Cuts at: 0 307 1477 2367 3177
Size: 307 1170 890 810
Fragments arranged by size:
1170 890 810 307
AflII C'TTAA_G
Cuts at: 0 414 3177
Size: 414 2763
AflIII A'CryG_T
Cuts at: 0 1646 3170 3177
Size: 1646 1524 7
Fragments arranged by size:
1646 1524 7
AleI CACnn'nnGTG
Cuts at: 0 2749 3177
Size: 2749 428
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 586 3177
Size: 586 2591
AlwNI CAG_nnn'CTG
Cuts at: 0 79 1966 2335 2947 3000 3091 3120 3177
Size: 79 1887 369 612 53 91 29 57
Fragments arranged by size:
1887 612 369 91 79 57 53 29
ApaI G_GGCC'C
Cuts at: 0 956 2013 2281 3034 3177
Size: 956 1057 268 753 143
Fragments arranged by size:
1057 956 753 268 143
ApaLI G'TGCA_C
Cuts at: 0 337 505 589 1507 1759 3166 3177
Size: 337 168 84 918 252 1407 11
Fragments arranged by size:
1407 918 337 252 168 84 11
AvaI C'yCGr_G
Cuts at: 0 693 728 949 2068 2097 2716 3027 3046
Size: 693 35 221 1119 29 619 311 19
Cuts at: 3046 3177
Size: 131
Fragments arranged by size:
1119 693 619 311 221 131 35 29
19
BanI G'GyrC_C
Cuts at: 0 36 491 679 697 773 834 963 981
Size: 36 455 188 18 76 61 129 18
Cuts at: 981 993 1244 1299 1916 2655 2980 3177
Size: 12 251 55 617 739 325 197
Fragments arranged by size:
739 617 455 325 251 197 188 129
76 61 55 36 18 18 12
BanII G_rGCy'C
Cuts at: 0 737 845 956 1112 1396 2013 2281 3034
Size: 737 108 111 156 284 617 268 753
Cuts at: 3034 3044 3054 3177
Size: 10 10 123
Fragments arranged by size:
753 737 617 284 268 156 123 111
108 10 10
BbeI G_GCGC'C
Cuts at: 0 40 495 683 967 997 1248 1303 3177
Size: 40 455 188 284 30 251 55 1874
Fragments arranged by size:
1874 455 284 251 188 55 40 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 738 3177
Size: 738 2439
BbvCI CC'TCA_GC
Cuts at: 0 1262 2690 3177
Size: 1262 1428 487
Fragments arranged by size:
1428 1262 487
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 729 763 1704 1738 3177
Size: 729 34 941 34 1439
Fragments arranged by size:
1439 941 729 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 97 260 374 709 1544 3177
Size: 97 163 114 335 835 1633
Fragments arranged by size:
1633 835 335 163 114 97
BlpI GC'TnA_GC
Cuts at: 0 2815 3177
Size: 2815 362
Bme1580I G_kGCm'C
Cuts at: 0 199 283 341 451 509 593 949 956
Size: 199 84 58 110 58 84 356 7
Cuts at: 956 1511 1763 2013 2086 2281 3034 3170 3177
Size: 555 252 250 73 195 753 136 7
Fragments arranged by size:
753 555 356 252 250 199 195 136
110 84 84 73 58 58 7 7
BmgBI CAC'GTC
Cuts at: 0 1649 2037 3177
Size: 1649 388 1140
Fragments arranged by size:
1649 1140 388
BmrI ACTGGGnnnn_n'
Cuts at: 0 96 717 3177
Size: 96 621 2460
Fragments arranged by size:
2460 621 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 952 2035 2146 2226 2443 2485 2518
Size: 763 189 1083 111 80 217 42 33
Cuts at: 2518 2551 2584 2764 2993 3038 3177
Size: 33 33 180 229 45 139
Fragments arranged by size:
1083 763 229 217 189 180 139 111
80 45 42 33 33 33
Bpu10I CC'TnA_GC
Cuts at: 0 839 1262 2690 3177
Size: 839 423 1428 487
Fragments arranged by size:
1428 839 487 423
BsaI GGTCTCn'nnnn_
Cuts at: 0 856 3177
Size: 856 2321
BsaAI yAC'GTr
Cuts at: 0 504 1224 1674 3177
Size: 504 720 450 1503
Fragments arranged by size:
1503 720 504 450
BsaHI Gr'CG_yC
Cuts at: 0 37 492 680 758 964 994 1245 1300
Size: 37 455 188 78 206 30 251 55
Cuts at: 1300 3177
Size: 1877
Fragments arranged by size:
1877 455 251 206 188 78 55 37
30
BsaWI w'CCGG_w
Cuts at: 0 134 1094 3177
Size: 134 960 2083
Fragments arranged by size:
2083 960 134
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 865 895 2856 2886 2903 2933 3177
Size: 865 30 1961 30 17 30 244
Fragments arranged by size:
1961 865 244 30 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 216 300 636 934 1028 1243 1386 1470
Size: 216 84 336 298 94 215 143 84
Cuts at: 1470 1806 2229 2707 2911 2990 3177
Size: 336 423 478 204 79 187
Fragments arranged by size:
478 423 336 336 298 216 215 204
187 143 94 84 84 79
BseYI C'CCAG_C
Cuts at: 0 968 1105 1951 1979 2173 2248 2254 2368
Size: 968 137 846 28 194 75 6 114
Cuts at: 2368 2940 3021 3091 3177
Size: 572 81 70 86
Fragments arranged by size:
968 846 572 194 137 114 86 81
75 70 28 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 646 1576 1816 2203 2371 2473 3177
Size: 139 507 930 240 387 168 102 704
Fragments arranged by size:
930 704 507 387 240 168 139 102
BsiEI CG_ry'CG
Cuts at: 0 1241 1884 2265 3177
Size: 1241 643 381 912
Fragments arranged by size:
1241 912 643 381
BsiHKAI G_wGCw'C
Cuts at: 0 341 509 565 593 845 938 1511 1763
Size: 341 168 56 28 252 93 573 252
Cuts at: 1763 3170 3177
Size: 1407 7
Fragments arranged by size:
1407 573 341 252 252 168 93 56
28 7
Bsp1286I G_dGCh'C
Cuts at: 0 199 283 341 451 509 565 593 737
Size: 199 84 58 110 58 56 28 144
Cuts at: 737 845 938 949 956 1112 1396 1511 1763
Size: 108 93 11 7 156 284 115 252
Cuts at: 1763 2013 2086 2281 3034 3044 3054 3170 3177
Size: 250 73 195 753 10 10 116 7
Fragments arranged by size:
753 284 252 250 199 195 156 144
116 115 110 108 93 84 73 58
58 56 28 11 10 10 7 7
BspEI T'CCGG_A
Cuts at: 0 1094 3177
Size: 1094 2083
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 578 662 773 1288 1424 1592 1748 1832
Size: 578 84 111 515 136 168 156 84
Cuts at: 1832 1876 2672 3177
Size: 44 796 505
Fragments arranged by size:
796 578 515 505 168 156 136 111
84 84 44
BsrBI CCG'CTC
Cuts at: 0 183 267 519 603 621 1353 1437 1605
Size: 183 84 252 84 18 732 84 168
Cuts at: 1605 1773 1791 2359 2681 2819 3177
Size: 168 18 568 322 138 358
Fragments arranged by size:
732 568 358 322 252 183 168 168
138 84 84 84 18 18
BsrFI r'CCGG_y
Cuts at: 0 39 343 511 1141 1972 2611 2899 3177
Size: 39 304 168 630 831 639 288 278
Fragments arranged by size:
831 639 630 304 288 278 168 39
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2805 3177
Size: 2805 372
BstEII G'GTnAC_C
Cuts at: 0 1275 2517 2583 3177
Size: 1275 1242 66 594
Fragments arranged by size:
1275 1242 594 66
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1203 2667 3177
Size: 1203 1464 510
Fragments arranged by size:
1464 1203 510
BstYI r'GATC_y
Cuts at: 0 323 3177
Size: 323 2854
Bsu36I CC'TnA_GG
Cuts at: 0 1921 3113 3177
Size: 1921 1192 64
Fragments arranged by size:
1921 1192 64
BtgI C'CryG_G
Cuts at: 0 707 830 866 1131 1235 2281 2489 2555
Size: 707 123 36 265 104 1046 208 66
Cuts at: 2555 2588 2606 3035 3177
Size: 33 18 429 142
Fragments arranged by size:
1046 707 429 265 208 142 123 104
66 36 33 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 905 3177
Size: 905 2272
BtsI GCAGTG_nn'
Cuts at: 0 1222 2294 3177
Size: 1222 1072 883
Fragments arranged by size:
1222 1072 883
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2613 2648 3177
Size: 2613 35 529
Fragments arranged by size:
2613 529 35
DraIII CAC_nnn'GTG
Cuts at: 0 1652 3177
Size: 1652 1525
EaeI y'GGCC_r
Cuts at: 0 366 1193 1238 1490 1536 1898 1937 2262
Size: 366 827 45 252 46 362 39 325
Cuts at: 2262 2360 2609 2613 3177
Size: 98 249 4 564
Fragments arranged by size:
827 564 366 362 325 252 249 98
46 45 39 4
EagI C'GGCC_G
Cuts at: 0 1238 2262 3177
Size: 1238 1024 915
Fragments arranged by size:
1238 1024 915
EarI CTCTTCn'nnn_
Cuts at: 0 541 3177
Size: 541 2636
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 874 1323 1867 2189 3177
Size: 874 449 544 322 988
Fragments arranged by size:
988 874 544 449 322
EcoICRI GAG'CTC
Cuts at: 0 843 3177
Size: 843 2334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 952 2035 2146 2226 2443 2485 2518
Size: 763 189 1083 111 80 217 42 33
Cuts at: 2518 2551 2584 2764 2993 3038 3177
Size: 33 33 180 229 45 139
Fragments arranged by size:
1083 763 229 217 189 180 139 111
80 45 42 33 33 33
EcoO109I rG'GnC_Cy
Cuts at: 0 76 1691 2010 2094 2157 2277 2332 2713
Size: 76 1615 319 84 63 120 55 381
Cuts at: 2713 3031 3117 3177
Size: 318 86 60
Fragments arranged by size:
1615 381 319 318 120 86 84 76
63 60 55
FseI GG_CCGG'CC
Cuts at: 0 2615 3177
Size: 2615 562
HaeII r_GCGC'y
Cuts at: 0 40 309 438 495 683 967 997 1248
Size: 40 269 129 57 188 284 30 251
Cuts at: 1248 1303 1479 1860 2119 2369 2939 3177
Size: 55 176 381 259 250 570 238
Fragments arranged by size:
570 381 284 269 259 251 250 238
188 176 129 57 55 40 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2903 2935 3177
Size: 2903 32 242
Fragments arranged by size:
2903 242 32
KasI G'GCGC_C
Cuts at: 0 36 491 679 963 993 1244 1299 3177
Size: 36 455 188 284 30 251 55 1878
Fragments arranged by size:
1878 455 284 251 188 55 36 30
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 1760 1844 2493 3177
Size: 674 1086 84 649 684
Fragments arranged by size:
1086 684 674 649 84
MscI TGG'CCA
Cuts at: 0 1195 1492 1538 3177
Size: 1195 297 46 1639
Fragments arranged by size:
1639 1195 297 46
MslI CAynn'nnrTG
Cuts at: 0 135 2749 3177
Size: 135 2614 428
Fragments arranged by size:
2614 428 135
MspA1I CmG'CkG
Cuts at: 0 614 709 771 887 1160 1169 1191 1237
Size: 614 95 62 116 273 9 22 46
Cuts at: 1237 1296 1503 1784 1914 2166 2283 2378 2689
Size: 59 207 281 130 252 117 95 311
Cuts at: 2689 3037 3162 3177
Size: 348 125 15
Fragments arranged by size:
614 348 311 281 273 252 207 130
125 117 116 95 95 62 59 46
22 15 9
NaeI GCC'GGC
Cuts at: 0 41 1143 1974 2613 3177
Size: 41 1102 831 639 564
Fragments arranged by size:
1102 831 639 564 41
NarI GG'CG_CC
Cuts at: 0 37 492 680 964 994 1245 1300 3177
Size: 37 455 188 284 30 251 55 1877
Fragments arranged by size:
1877 455 284 251 188 55 37 30
NgoMIV G'CCGG_C
Cuts at: 0 39 1141 1972 2611 3177
Size: 39 1102 831 639 566
Fragments arranged by size:
1102 831 639 566 39
PasI CC'CwG_GG
Cuts at: 0 2154 2335 3177
Size: 2154 181 842
Fragments arranged by size:
2154 842 181
PflMI CCAn_nnn'nTGG
Cuts at: 0 2335 2607 3177
Size: 2335 272 570
Fragments arranged by size:
2335 570 272
PfoI T'CCnGG_A
Cuts at: 0 929 2756 3177
Size: 929 1827 421
Fragments arranged by size:
1827 929 421
PmlI CAC'GTG
Cuts at: 0 504 1224 1674 3177
Size: 504 720 450 1503
Fragments arranged by size:
1503 720 504 450
PshAI GACnn'nnGTC
Cuts at: 0 2524 2590 3177
Size: 2524 66 587
Fragments arranged by size:
2524 587 66
PspOMI G'GGCC_C
Cuts at: 0 952 2009 2277 3030 3177
Size: 952 1057 268 753 147
Fragments arranged by size:
1057 952 753 268 147
PspXI vC'TCGA_Gb
Cuts at: 0 2068 3177
Size: 2068 1109
PstI C_TGCA'G
Cuts at: 0 323 770 1913 2408 2996 3177
Size: 323 447 1143 495 588 181
Fragments arranged by size:
1143 588 495 447 323 181
PvuII CAG'CTG
Cuts at: 0 1169 1191 2166 2689 3162 3177
Size: 1169 22 975 523 473 15
Fragments arranged by size:
1169 975 523 473 22 15
SacI G_AGCT'C
Cuts at: 0 845 3177
Size: 845 2332
SacII CC_GC'GG
Cuts at: 0 710 1238 2284 3038 3177
Size: 710 528 1046 754 139
Fragments arranged by size:
1046 754 710 528 139
SapI GCTCTTCn'nnn_
Cuts at: 0 541 3177
Size: 541 2636
SfcI C'TryA_G
Cuts at: 0 319 766 1909 2404 2992 3177
Size: 319 447 1143 495 588 185
Fragments arranged by size:
1143 588 495 447 319 185
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 374 1544 3177
Size: 374 1170 1633
Fragments arranged by size:
1633 1170 374
SfoI GGC'GCC
Cuts at: 0 38 493 681 965 995 1246 1301 3177
Size: 38 455 188 284 30 251 55 1876
Fragments arranged by size:
1876 455 284 251 188 55 38 30
SgrAI Cr'CCGG_yG
Cuts at: 0 511 3177
Size: 511 2666
SmaI CCC'GGG
Cuts at: 0 695 730 951 2718 3048 3177
Size: 695 35 221 1767 330 129
Fragments arranged by size:
1767 695 330 221 129 35
SmlI C'TyrA_G
Cuts at: 0 414 2068 3177
Size: 414 1654 1109
Fragments arranged by size:
1654 1109 414
StuI AGG'CCT
Cuts at: 0 298 466 1468 1636 3177
Size: 298 168 1002 168 1541
Fragments arranged by size:
1541 1002 298 168 168
StyI C'CwwG_G
Cuts at: 0 209 293 461 998 3177
Size: 209 84 168 537 2179
Fragments arranged by size:
2179 537 209 168 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 655 1825 2038 2673 3177
Size: 655 1170 213 635 504
Fragments arranged by size:
1170 655 635 504 213
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 109 873 1678 2748 2952 3177
Size: 109 764 805 1070 204 225
Fragments arranged by size:
1070 805 764 225 204 109
XhoI C'TCGA_G
Cuts at: 0 2068 3177
Size: 2068 1109
XmaI C'CCGG_G
Cuts at: 0 693 728 949 2716 3046 3177
Size: 693 35 221 1767 330 131
Fragments arranged by size:
1767 693 330 221 131 35
XmnI GAAnn'nnTTC
Cuts at: 0 550 2867 3177
Size: 550 2317 310
Fragments arranged by size:
2317 550 310
Enzymes that do cut:
AarI AfeI AflII AflIII AleI AlfI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FseI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV PasI PflMI PfoI PmlI PshAI PspOMI
PspXI PstI PvuII SacI SacII SapI SfcI SfiI
SfoI SgrAI SmaI SmlI StuI StyI TaqII XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AgeI AhdI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI
BclI BfrBI BglII BmtI BplI BpuEI BsaBI BsiWI
BsmI BsmBI BspHI BsrDI BsrGI BssHII BssSI BstBI
BstZ17I ClaI DraI DrdI EcoNI EcoRI EcoRV FalI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PmeI PpiI PpuMI PsiI PsrI PvuI RsrII
SalI SanDI SbfI ScaI SexAI SgfI SnaBI SpeI
SphI SrfI SspI SwaI TatI TstI Tth111I XbaI
ZraI