Restriction map for
pFN21AE5819
(Linear) (Six-base) MAPSORT of: KIEE5819.seq Check: 899 from: 1 to: 3189
KIEE5819.orf
With 182 enzymes: SgfI *
December 5, 2014 13:12 ..
Acc65I G'GTAC_C
Cuts at: 0 1794 3189
Size: 1794 1395
AclI AA'CG_TT
Cuts at: 0 2447 3189
Size: 2447 742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 722 992 1028 1596 2206 2759 3189
Size: 184 538 270 36 568 610 553 430
Fragments arranged by size:
610 568 553 538 430 270 184 36
AflII C'TTAA_G
Cuts at: 0 2584 3189
Size: 2584 605
AflIII A'CryG_T
Cuts at: 0 1788 3189
Size: 1788 1401
AgeI A'CCGG_T
Cuts at: 0 661 3189
Size: 661 2528
AhdI GACnn_n'nnGTC
Cuts at: 0 2540 3189
Size: 2540 649
AleI CACnn'nnGTG
Cuts at: 0 1722 3066 3189
Size: 1722 1344 123
Fragments arranged by size:
1722 1344 123
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2144 3189
Size: 2144 1045
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 97 129 3019 3051 3189
Size: 97 32 2890 32 138
Fragments arranged by size:
2890 138 97 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 100 162 350 1051 3189
Size: 100 62 188 701 2138
Fragments arranged by size:
2138 701 188 100 62
ApaI G_GGCC'C
Cuts at: 0 181 3189
Size: 181 3008
ApoI r'AATT_y
Cuts at: 0 1219 3189
Size: 1219 1970
AvaI C'yCGr_G
Cuts at: 0 192 220 450 757 1122 2506 3189
Size: 192 28 230 307 365 1384 683
Fragments arranged by size:
1384 683 365 307 230 192 28
BanI G'GyrC_C
Cuts at: 0 1794 2077 2779 3150 3189
Size: 1794 283 702 371 39
Fragments arranged by size:
1794 702 371 283 39
BanII G_rGCy'C
Cuts at: 0 181 199 230 722 758 1123 1465 3013
Size: 181 18 31 492 36 365 342 1548
Cuts at: 3013 3061 3189
Size: 48 128
Fragments arranged by size:
1548 492 365 342 181 128 48 36
31 18
BbeI G_GCGC'C
Cuts at: 0 2081 3189
Size: 2081 1108
BbsI GAAGACnn'nnnn_
Cuts at: 0 880 1016 1191 2697 3189
Size: 880 136 175 1506 492
Fragments arranged by size:
1506 880 492 175 136
BbvCI CC'TCA_GC
Cuts at: 0 798 3189
Size: 798 2391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 946 980 2326 2360 3189
Size: 946 34 1346 34 829
Fragments arranged by size:
1346 946 829 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1994 3189
Size: 1994 1195
BfrBI ATG'CAT
Cuts at: 0 2048 2993 3189
Size: 2048 945 196
Fragments arranged by size:
2048 945 196
BglI GCCn_nnn'nGGC
Cuts at: 0 431 808 3189
Size: 431 377 2381
Fragments arranged by size:
2381 431 377
BlpI GC'TnA_GC
Cuts at: 0 827 1117 2251 3012 3189
Size: 827 290 1134 761 177
Fragments arranged by size:
1134 827 761 290 177
Bme1580I G_kGCm'C
Cuts at: 0 181 457 3153 3189
Size: 181 276 2696 36
Fragments arranged by size:
2696 276 181 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 1656 1837 3189
Size: 1656 181 1352
Fragments arranged by size:
1656 1352 181
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 92 2190 3189
Size: 92 2098 999
Fragments arranged by size:
2098 999 92
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1504 2154 2185 2835 3189
Size: 493 1011 650 31 650 354
Fragments arranged by size:
1011 650 650 493 354 31
Bpu10I CC'TnA_GC
Cuts at: 0 798 1464 1984 3088 3189
Size: 798 666 520 1104 101
Fragments arranged by size:
1104 798 666 520 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1000 2425 3189
Size: 1000 1425 764
Fragments arranged by size:
1425 1000 764
BsaI GGTCTCn'nnnn_
Cuts at: 0 934 3118 3189
Size: 934 2184 71
Fragments arranged by size:
2184 934 71
BsaAI yAC'GTr
Cuts at: 0 1791 3189
Size: 1791 1398
BsaHI Gr'CG_yC
Cuts at: 0 124 2078 3189
Size: 124 1954 1111
Fragments arranged by size:
1954 1111 124
BsaWI w'CCGG_w
Cuts at: 0 661 2273 3189
Size: 661 1612 916
Fragments arranged by size:
1612 916 661
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 97 127 472 502 709 739 894 924
Size: 97 30 345 30 207 30 155 30
Cuts at: 924 1124 1154 2503 2533 3019 3049 3189
Size: 200 30 1349 30 486 30 140
Fragments arranged by size:
1349 486 345 207 200 155 140 97
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 899 1145 1223 1226 1863 2524 3027
Size: 238 661 246 78 3 637 661 503
Cuts at: 3027 3111 3147 3189
Size: 84 36 42
Fragments arranged by size:
661 661 637 503 246 238 84 78
42 36 3
BseYI C'CCAG_C
Cuts at: 0 413 2073 2871 2942 3083 3108 3189
Size: 413 1660 798 71 141 25 81
Fragments arranged by size:
1660 798 413 141 81 71 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1064 1243 2077 3189
Size: 1064 179 834 1112
Fragments arranged by size:
1112 1064 834 179
BsiHKAI G_wGCw'C
Cuts at: 0 722 2339 3013 3189
Size: 722 1617 674 176
Fragments arranged by size:
1617 722 674 176
BsmBI CGTCTCn'nnnn_
Cuts at: 0 952 3189
Size: 952 2237
Bsp1286I G_dGCh'C
Cuts at: 0 181 199 230 457 722 758 1123 1465
Size: 181 18 31 227 265 36 365 342
Cuts at: 1465 2339 3013 3061 3153 3189
Size: 874 674 48 92 36
Fragments arranged by size:
874 674 365 342 265 227 181 92
48 36 36 31 18
BspHI T'CATG_A
Cuts at: 0 1281 1529 3189
Size: 1281 248 1660
Fragments arranged by size:
1660 1281 248
BsrBI CCG'CTC
Cuts at: 0 2161 3189
Size: 2161 1028
BsrDI GCAATG_nn'
Cuts at: 0 1798 3180 3189
Size: 1798 1382 9
Fragments arranged by size:
1798 1382 9
BsrFI r'CCGG_y
Cuts at: 0 661 2390 3189
Size: 661 1729 799
Fragments arranged by size:
1729 799 661
BstAPI GCAn_nnn'nTGC
Cuts at: 0 59 817 1310 2247 2939 3189
Size: 59 758 493 937 692 250
Fragments arranged by size:
937 758 692 493 250 59
BstXI CCAn_nnnn'nTGG
Cuts at: 0 421 471 1275 1996 3189
Size: 421 50 804 721 1193
Fragments arranged by size:
1193 804 721 421 50
Bsu36I CC'TnA_GG
Cuts at: 0 383 2144 3189
Size: 383 1761 1045
Fragments arranged by size:
1761 1045 383
BtgI C'CryG_G
Cuts at: 0 376 2116 2843 3189
Size: 376 1740 727 346
Fragments arranged by size:
1740 727 376 346
BtsI GCAGTG_nn'
Cuts at: 0 924 2094 2921 3189
Size: 924 1170 827 268
Fragments arranged by size:
1170 924 827 268
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 486 521 3189
Size: 486 35 2668
Fragments arranged by size:
2668 486 35
EaeI y'GGCC_r
Cuts at: 0 432 551 2972 3189
Size: 432 119 2421 217
Fragments arranged by size:
2421 432 217 119
EarI CTCTTCn'nnn_
Cuts at: 0 1169 1753 2275 3189
Size: 1169 584 522 914
Fragments arranged by size:
1169 914 584 522
EcoICRI GAG'CTC
Cuts at: 0 720 3011 3189
Size: 720 2291 178
Fragments arranged by size:
2291 720 178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 493 722 992 1028 1504 1596 2154
Size: 184 309 229 270 36 476 92 558
Cuts at: 2154 2185 2206 2759 2835 3189
Size: 31 21 553 76 354
Fragments arranged by size:
558 553 476 354 309 270 229 184
92 76 36 31 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 649 1567 3166 3189
Size: 649 918 1599 23
Fragments arranged by size:
1599 918 649 23
EcoO109I rG'GnC_Cy
Cuts at: 0 177 794 1560 2714 3189
Size: 177 617 766 1154 475
Fragments arranged by size:
1154 766 617 475 177
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2734 3189
Size: 2734 455
HaeII r_GCGC'y
Cuts at: 0 2081 3182 3189
Size: 2081 1101 7
Fragments arranged by size:
2081 1101 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 472 504 1942 1974 2503 2526 2535 2558
Size: 472 32 1438 32 529 23 9 23
Cuts at: 2558 3043 3075 3189
Size: 485 32 114
Fragments arranged by size:
1438 529 485 472 114 32 32 32
23 23 9
HincII GTy'rAC
Cuts at: 0 1428 3189
Size: 1428 1761
KasI G'GCGC_C
Cuts at: 0 2077 3189
Size: 2077 1112
KpnI G_GTAC'C
Cuts at: 0 1798 3189
Size: 1798 1391
MscI TGG'CCA
Cuts at: 0 434 553 2974 3189
Size: 434 119 2421 215
Fragments arranged by size:
2421 434 215 119
MslI CAynn'nnrTG
Cuts at: 0 1722 2126 3066 3189
Size: 1722 404 940 123
Fragments arranged by size:
1722 940 404 123
MspA1I CmG'CkG
Cuts at: 0 1048 1433 1588 1722 2118 2845 3066 3108
Size: 1048 385 155 134 396 727 221 42
Cuts at: 3108 3189
Size: 81
Fragments arranged by size:
1048 727 396 385 221 155 134 81
42
NarI GG'CG_CC
Cuts at: 0 2078 3189
Size: 2078 1111
NcoI C'CATG_G
Cuts at: 0 376 3189
Size: 376 2813
NsiI A_TGCA'T
Cuts at: 0 2050 2995 3189
Size: 2050 945 194
Fragments arranged by size:
2050 945 194
NspI r_CATG'y
Cuts at: 0 25 2766 3189
Size: 25 2741 423
Fragments arranged by size:
2741 423 25
PflMI CCAn_nnn'nTGG
Cuts at: 0 534 3189
Size: 534 2655
PfoI T'CCnGG_A
Cuts at: 0 244 587 1094 2189 2363 2397 2480 3189
Size: 244 343 507 1095 174 34 83 709
Fragments arranged by size:
1095 709 507 343 244 174 83 34
PmlI CAC'GTG
Cuts at: 0 1791 3189
Size: 1791 1398
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 1372 1404 3019 3051 3189
Size: 97 32 1243 32 1615 32 138
Fragments arranged by size:
1615 1243 138 97 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1560 2714 3189
Size: 1560 1154 475
Fragments arranged by size:
1560 1154 475
PshAI GACnn'nnGTC
Cuts at: 0 1277 3189
Size: 1277 1912
PsiI TTA'TAA
Cuts at: 0 1364 3189
Size: 1364 1825
PspOMI G'GGCC_C
Cuts at: 0 177 3189
Size: 177 3012
PstI C_TGCA'G
Cuts at: 0 55 84 524 773 1625 2569 2629 2664
Size: 55 29 440 249 852 944 60 35
Cuts at: 2664 3189
Size: 525
Fragments arranged by size:
944 852 525 440 249 60 55 35
29
PvuII CAG'CTG
Cuts at: 0 1048 1722 3066 3189
Size: 1048 674 1344 123
Fragments arranged by size:
1344 1048 674 123
SacI G_AGCT'C
Cuts at: 0 722 3013 3189
Size: 722 2291 176
Fragments arranged by size:
2291 722 176
SacII CC_GC'GG
Cuts at: 0 2119 2846 3189
Size: 2119 727 343
Fragments arranged by size:
2119 727 343
SanDI GG'GwC_CC
Cuts at: 0 2714 3189
Size: 2714 475
SbfI CC_TGCA'GG
Cuts at: 0 524 3189
Size: 524 2665
ScaI AGT'ACT
Cuts at: 0 1483 3189
Size: 1483 1706
SexAI A'CCwGG_T
Cuts at: 0 1562 2024 2306 3189
Size: 1562 462 282 883
Fragments arranged by size:
1562 883 462 282
SfcI C'TryA_G
Cuts at: 0 51 80 520 769 821 1621 2565 2625
Size: 51 29 440 249 52 800 944 60
Cuts at: 2625 2660 3189
Size: 35 529
Fragments arranged by size:
944 800 529 440 249 60 52 51
35 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 431 3189
Size: 431 2758
SfoI GGC'GCC
Cuts at: 0 2079 3189
Size: 2079 1110
SmaI CCC'GGG
Cuts at: 0 194 452 759 1124 3189
Size: 194 258 307 365 2065
Fragments arranged by size:
2065 365 307 258 194
SmlI C'TyrA_G
Cuts at: 0 1015 2404 2584 3189
Size: 1015 1389 180 605
Fragments arranged by size:
1389 1015 605 180
StuI AGG'CCT
Cuts at: 0 644 1868 2515 3189
Size: 644 1224 647 674
Fragments arranged by size:
1224 674 647 644
StyI C'CwwG_G
Cuts at: 0 29 376 950 1995 2709 3167 3189
Size: 29 347 574 1045 714 458 22
Fragments arranged by size:
1045 714 574 458 347 29 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1675 1744 3189
Size: 1675 69 1445
Fragments arranged by size:
1675 1445 69
TatI w'GTAC_w
Cuts at: 0 1481 1739 3189
Size: 1481 258 1450
Fragments arranged by size:
1481 1450 258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1124 1156 1915 1947 3189
Size: 1124 32 759 32 1242
Fragments arranged by size:
1242 1124 759 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 468 3189
Size: 468 2721
XmaI C'CCGG_G
Cuts at: 0 192 450 757 1122 3189
Size: 192 258 307 365 2067
Fragments arranged by size:
2067 365 307 258 192
XmnI GAAnn'nnTTC
Cuts at: 0 1651 2688 3189
Size: 1651 1037 501
Fragments arranged by size:
1651 1037 501
Enzymes that do cut:
Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BsrBI BsrDI BsrFI BstAPI BstXI Bsu36I
BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII KasI KpnI MscI
MslI MspA1I NarI NcoI NsiI NspI PflMI PfoI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuII
SacI SacII SanDI SbfI ScaI SexAI SfcI SfiI
SfoI SmaI SmlI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AfeI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BclI BglII BmgBI BmtI BsaBI
BsiEI BsiWI BsmI BspEI BspMI BsrGI BssHII BssSI
BstBI BstEII BstYI BstZ17I BtgZI ClaI DraI DraIII
DrdI EagI EciI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI MfeI MluI MmeI NaeI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PmeI PspXI
PsrI PvuI RsrII SalI SapI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
ZraI