Restriction map for
pFN21AE5812
(Linear) (Six-base) MAPSORT of: KIEE5812.seq Check: 1710 from: 1 to: 3609
KIEE5812.orf
With 182 enzymes: SgfI *
December 5, 2014 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 570 3609
Size: 570 3039
AatII G_ACGT'C
Cuts at: 0 3434 3609
Size: 3434 175
Acc65I G'GTAC_C
Cuts at: 0 2201 3609
Size: 2201 1408
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1041 2154 3609
Size: 1041 1113 1455
Fragments arranged by size:
1455 1113 1041
AfeI AGC'GCT
Cuts at: 0 700 3609
Size: 700 2909
AflIII A'CryG_T
Cuts at: 0 3329 3609
Size: 3329 280
AhdI GACnn_n'nnGTC
Cuts at: 0 603 1674 3609
Size: 603 1071 1935
Fragments arranged by size:
1935 1071 603
AleI CACnn'nnGTG
Cuts at: 0 1249 2396 3609
Size: 1249 1147 1213
Fragments arranged by size:
1249 1213 1147
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3124 3609
Size: 3124 485
AlwNI CAG_nnn'CTG
Cuts at: 0 369 1064 1611 1940 2238 2667 3046 3609
Size: 369 695 547 329 298 429 379 563
Fragments arranged by size:
695 563 547 429 379 369 329 298
ApaI G_GGCC'C
Cuts at: 0 91 123 197 278 1593 1923 2097 2908
Size: 91 32 74 81 1315 330 174 811
Cuts at: 2908 3609
Size: 701
Fragments arranged by size:
1315 811 701 330 174 91 81 74
32
ApaLI G'TGCA_C
Cuts at: 0 2257 2556 3298 3609
Size: 2257 299 742 311
Fragments arranged by size:
2257 742 311 299
AvaI C'yCGr_G
Cuts at: 0 162 380 593 919 1356 1643 1961 2159
Size: 162 218 213 326 437 287 318 198
Cuts at: 2159 3233 3316 3609
Size: 1074 83 293
Fragments arranged by size:
1074 437 326 318 293 287 218 213
198 162 83
BamHI G'GATC_C
Cuts at: 0 560 1731 2685 3609
Size: 560 1171 954 924
Fragments arranged by size:
1171 954 924 560
BanI G'GyrC_C
Cuts at: 0 481 936 1459 1927 2201 2463 2832 3609
Size: 481 455 523 468 274 262 369 777
Fragments arranged by size:
777 523 481 468 455 369 274 262
BanII G_rGCy'C
Cuts at: 0 91 113 123 169 197 278 304 818
Size: 91 22 10 46 28 81 26 514
Cuts at: 818 896 1593 1923 1955 2097 2477 2651 2908
Size: 78 697 330 32 142 380 174 257
Cuts at: 2908 2988 3485 3609
Size: 80 497 124
Fragments arranged by size:
697 514 497 380 330 257 174 142
124 91 81 80 78 46 32 28
26 22 10
BbeI G_GCGC'C
Cuts at: 0 2467 3609
Size: 2467 1142
BbsI GAAGACnn'nnnn_
Cuts at: 0 1486 3476 3609
Size: 1486 1990 133
Fragments arranged by size:
1990 1486 133
BbvCI CC'TCA_GC
Cuts at: 0 250 2612 2677 3609
Size: 250 2362 65 932
Fragments arranged by size:
2362 932 250 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1065 1099 3609
Size: 1065 34 2510
Fragments arranged by size:
2510 1065 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1149 3609
Size: 1149 2460
BglI GCCn_nnn'nGGC
Cuts at: 0 100 469 1000 1270 2110 2415 2513 3609
Size: 100 369 531 270 840 305 98 1096
Fragments arranged by size:
1096 840 531 369 305 270 100 98
BglII A'GATC_T
Cuts at: 0 1511 3609
Size: 1511 2098
BlpI GC'TnA_GC
Cuts at: 0 521 2209 2440 2629 2938 3609
Size: 521 1688 231 189 309 671
Fragments arranged by size:
1688 671 521 309 231 189
Bme1580I G_kGCm'C
Cuts at: 0 91 123 197 278 941 1349 1462 1534
Size: 91 32 74 81 663 408 113 72
Cuts at: 1534 1593 1923 1930 2097 2261 2560 2908 2969
Size: 59 330 7 167 164 299 348 61
Cuts at: 2969 3302 3569 3609
Size: 333 267 40
Fragments arranged by size:
663 408 348 333 330 299 267 167
164 113 91 81 74 72 61 59
40 32 7
BmgBI CAC'GTC
Cuts at: 0 861 1671 3609
Size: 861 810 1938
Fragments arranged by size:
1938 861 810
BmrI ACTGGGnnnn_n'
Cuts at: 0 228 1341 2029 3609
Size: 228 1113 688 1580
Fragments arranged by size:
1580 1113 688 228
BmtI G_CTAG'C
Cuts at: 0 1237 3609
Size: 1237 2372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 964 979 1057 1147 1312 2032 2515 2689
Size: 964 15 78 90 165 720 483 174
Cuts at: 2689 2755 2764 2788 3609
Size: 66 9 24 821
Fragments arranged by size:
964 821 720 483 174 165 90 78
66 24 15 9
Bpu10I CC'TnA_GC
Cuts at: 0 107 250 359 493 2612 2677 3609
Size: 107 143 109 134 2119 65 932
Fragments arranged by size:
2119 932 143 134 109 107 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 2932 3609
Size: 952 1980 677
Fragments arranged by size:
1980 952 677
BsaI GGTCTCn'nnnn_
Cuts at: 0 1573 2116 2951 3609
Size: 1573 543 835 658
Fragments arranged by size:
1573 835 658 543
BsaAI yAC'GTr
Cuts at: 0 2256 2933 3261 3609
Size: 2256 677 328 348
Fragments arranged by size:
2256 677 348 328
BsaHI Gr'CG_yC
Cuts at: 0 2464 3431 3609
Size: 2464 967 178
Fragments arranged by size:
2464 967 178
BsaWI w'CCGG_w
Cuts at: 0 1625 2353 3609
Size: 1625 728 1256
Fragments arranged by size:
1625 1256 728
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 391 421 1780 1810 1831 1861 2581 2611
Size: 391 30 1359 30 21 30 720 30
Cuts at: 2611 2695 2725 2948 2978 3535 3565 3609
Size: 84 30 223 30 557 30 44
Fragments arranged by size:
1359 720 557 391 223 84 44 30
30 30 30 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 432 728 2089 2716 2773 3609
Size: 432 296 1361 627 57 836
Fragments arranged by size:
1361 836 627 432 296 57
BseYI C'CCAG_C
Cuts at: 0 54 60 90 112 123 179 792 1611
Size: 54 6 30 22 11 56 613 819
Cuts at: 1611 2043 2090 2528 2605 2789 2836 2901 3013
Size: 432 47 438 77 184 47 65 112
Cuts at: 3013 3191 3609
Size: 178 418
Fragments arranged by size:
819 613 438 432 418 184 178 112
77 65 56 54 47 47 30 22
11 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 264 593 1204 1934 2896 3202 3283 3400
Size: 264 329 611 730 962 306 81 117
Cuts at: 3400 3609
Size: 209
Fragments arranged by size:
962 730 611 329 306 264 209 117
81
BsiEI CG_ry'CG
Cuts at: 0 852 1587 1807 3609
Size: 852 735 220 1802
Fragments arranged by size:
1802 852 735 220
BsiHKAI G_wGCw'C
Cuts at: 0 169 818 950 1078 2261 2560 3302 3609
Size: 169 649 132 128 1183 299 742 307
Fragments arranged by size:
1183 742 649 307 299 169 132 128
BsmI GAATG_Cn'
Cuts at: 0 821 3609
Size: 821 2788
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1684 3609
Size: 1684 1925
Bsp1286I G_dGCh'C
Cuts at: 0 91 113 123 169 197 278 304 818
Size: 91 22 10 46 28 81 26 514
Cuts at: 818 896 941 950 1078 1349 1462 1534 1593
Size: 78 45 9 128 271 113 72 59
Cuts at: 1593 1923 1930 1955 2097 2261 2477 2560 2651
Size: 330 7 25 142 164 216 83 91
Cuts at: 2651 2908 2969 2988 3302 3485 3569 3609
Size: 257 61 19 314 183 84 40
Fragments arranged by size:
514 330 314 271 257 216 183 164
142 128 113 91 91 84 83 81
78 72 61 59 46 45 40 28
26 25 22 19 10 9 7
BspEI T'CCGG_A
Cuts at: 0 1625 3609
Size: 1625 1984
BspHI T'CATG_A
Cuts at: 0 1280 3609
Size: 1280 2329
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 570 593 2312 3609
Size: 570 23 1719 1297
Fragments arranged by size:
1719 1297 570 23
BsrBI CCG'CTC
Cuts at: 0 422 2031 2291 3081 3321 3609
Size: 422 1609 260 790 240 288
Fragments arranged by size:
1609 790 422 288 260 240
BsrDI GCAATG_nn'
Cuts at: 0 3050 3609
Size: 3050 559
BsrFI r'CCGG_y
Cuts at: 0 332 761 1462 1582 2204 2414 2460 3424
Size: 332 429 701 120 622 210 46 964
Cuts at: 3424 3609
Size: 185
Fragments arranged by size:
964 701 622 429 332 210 185 120
46
BssSI C'ACGA_G
Cuts at: 0 206 436 1089 1687 1991 2322 3609
Size: 206 230 653 598 304 331 1287
Fragments arranged by size:
1287 653 598 331 304 230 206
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1850 2238 3306 3378 3609
Size: 1850 388 1068 72 231
Fragments arranged by size:
1850 1068 388 231 72
BstEII G'GTnAC_C
Cuts at: 0 1707 3609
Size: 1707 1902
BstXI CCAn_nnnn'nTGG
Cuts at: 0 31 1436 2337 2733 3129 3609
Size: 31 1405 901 396 396 480
Fragments arranged by size:
1405 901 480 396 396 31
BstYI r'GATC_y
Cuts at: 0 560 1511 1731 1829 2685 2855 3609
Size: 560 951 220 98 856 170 754
Fragments arranged by size:
951 856 754 560 220 170 98
Bsu36I CC'TnA_GG
Cuts at: 0 1933 3274 3609
Size: 1933 1341 335
Fragments arranged by size:
1933 1341 335
BtgI C'CryG_G
Cuts at: 0 316 1268 1997 2477 3353 3609
Size: 316 952 729 480 876 256
Fragments arranged by size:
952 876 729 480 316 256
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3359 3609
Size: 3359 250
BtsI GCAGTG_nn'
Cuts at: 0 1010 1202 2776 3569 3609
Size: 1010 192 1574 793 40
Fragments arranged by size:
1574 1010 793 192 40
DraI TTT'AAA
Cuts at: 0 1488 3609
Size: 1488 2121
DraIII CAC_nnn'GTG
Cuts at: 0 3306 3609
Size: 3306 303
DrdI GACnn_nn'nnGTC
Cuts at: 0 1170 3609
Size: 1170 2439
EaeI y'GGCC_r
Cuts at: 0 677 708 1211 1496 1584 1804 2067 3210
Size: 677 31 503 285 88 220 263 1143
Cuts at: 3210 3609
Size: 399
Fragments arranged by size:
1143 677 503 399 285 263 220 88
31
EagI C'GGCC_G
Cuts at: 0 1584 1804 3609
Size: 1584 220 1805
Fragments arranged by size:
1805 1584 220
EarI CTCTTCn'nnn_
Cuts at: 0 7 910 1627 1879 2639 3609
Size: 7 903 717 252 760 970
Fragments arranged by size:
970 903 760 717 252 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1506 1896 2280 3609
Size: 1506 390 384 1329
Fragments arranged by size:
1506 1329 390 384
EcoICRI GAG'CTC
Cuts at: 0 167 816 3609
Size: 167 649 2793
Fragments arranged by size:
2793 649 167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 964 979 1041 1057 1147 1312 2032 2154
Size: 964 15 62 16 90 165 720 122
Cuts at: 2154 2515 2689 2755 2764 2788 3609
Size: 361 174 66 9 24 821
Fragments arranged by size:
964 821 720 361 174 165 122 90
66 62 24 16 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 710 2428 2680 3609
Size: 710 1718 252 929
Fragments arranged by size:
1718 929 710 252
EcoO109I rG'GnC_Cy
Cuts at: 0 87 120 193 275 789 882 1032 1647
Size: 87 33 73 82 514 93 150 615
Cuts at: 1647 1919 1920 2226 2454 2538 2673 2905 3609
Size: 272 1 306 228 84 135 232 704
Fragments arranged by size:
704 615 514 306 272 232 228 150
135 93 87 84 82 73 33 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1500 1851 3609
Size: 1500 351 1758
Fragments arranged by size:
1758 1500 351
FseI GG_CCGG'CC
Cuts at: 0 336 3609
Size: 336 3273
FspI TGC'GCA
Cuts at: 0 3493 3609
Size: 3493 116
HaeII r_GCGC'y
Cuts at: 0 148 257 702 2196 2216 2437 2467 2767
Size: 148 109 445 1494 20 221 30 300
Cuts at: 2767 3609
Size: 842
Fragments arranged by size:
1494 842 445 300 221 148 109 30
20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 589 621 1660 1692 2581 2613 3609
Size: 589 32 1039 32 889 32 996
Fragments arranged by size:
1039 996 889 589 32 32 32
HincII GTy'rAC
Cuts at: 0 1395 3609
Size: 1395 2214
KasI G'GCGC_C
Cuts at: 0 2463 3609
Size: 2463 1146
KpnI G_GTAC'C
Cuts at: 0 2205 3609
Size: 2205 1404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1034 3609
Size: 1034 2575
MscI TGG'CCA
Cuts at: 0 1498 3609
Size: 1498 2111
MslI CAynn'nnrTG
Cuts at: 0 685 1249 1537 2338 2396 3031 3121 3439
Size: 685 564 288 801 58 635 90 318
Cuts at: 3439 3609
Size: 170
Fragments arranged by size:
801 685 635 564 318 288 170 90
58
MspA1I CmG'CkG
Cuts at: 0 366 444 548 741 1611 1762 2043 2493
Size: 366 78 104 193 870 151 281 450
Cuts at: 2493 2720 2793 3609
Size: 227 73 816
Fragments arranged by size:
870 816 450 366 281 227 193 151
104 78 73
NaeI GCC'GGC
Cuts at: 0 334 2462 3426 3609
Size: 334 2128 964 183
Fragments arranged by size:
2128 964 334 183
NarI GG'CG_CC
Cuts at: 0 2464 3609
Size: 2464 1145
NcoI C'CATG_G
Cuts at: 0 3353 3609
Size: 3353 256
NgoMIV G'CCGG_C
Cuts at: 0 332 2460 3424 3609
Size: 332 2128 964 185
Fragments arranged by size:
2128 964 332 185
NheI G'CTAG_C
Cuts at: 0 1233 3609
Size: 1233 2376
NspI r_CATG'y
Cuts at: 0 1017 3333 3609
Size: 1017 2316 276
Fragments arranged by size:
2316 1017 276
PasI CC'CwG_GG
Cuts at: 0 975 1923 2651 3569 3609
Size: 975 948 728 918 40
Fragments arranged by size:
975 948 918 728 40
PciI A'CATG_T
Cuts at: 0 3329 3609
Size: 3329 280
PflMI CCAn_nnn'nTGG
Cuts at: 0 3046 3609
Size: 3046 563
PfoI T'CCnGG_A
Cuts at: 0 1124 2585 3609
Size: 1124 1461 1024
Fragments arranged by size:
1461 1124 1024
PmlI CAC'GTG
Cuts at: 0 2256 2933 3609
Size: 2256 677 676
Fragments arranged by size:
2256 677 676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 496 528 3609
Size: 496 32 3081
Fragments arranged by size:
3081 496 32
PpuMI rG'GwC_Cy
Cuts at: 0 789 1647 2454 2538 3609
Size: 789 858 807 84 1071
Fragments arranged by size:
1071 858 807 789 84
PsiI TTA'TAA
Cuts at: 0 1826 3609
Size: 1826 1783
PspOMI G'GGCC_C
Cuts at: 0 87 119 193 274 1589 1919 2093 2904
Size: 87 32 74 81 1315 330 174 811
Cuts at: 2904 3609
Size: 705
Fragments arranged by size:
1315 811 705 330 174 87 81 74
32
PspXI vC'TCGA_Gb
Cuts at: 0 3316 3609
Size: 3316 293
PstI C_TGCA'G
Cuts at: 0 581 604 881 1329 2042 2234 2375 3113
Size: 581 23 277 448 713 192 141 738
Cuts at: 3113 3374 3609
Size: 261 235
Fragments arranged by size:
738 713 581 448 277 261 235 192
141 23
PvuII CAG'CTG
Cuts at: 0 366 741 1611 1762 2043 2493 2793 3609
Size: 366 375 870 151 281 450 300 816
Fragments arranged by size:
870 816 450 375 366 300 281 151
SacI G_AGCT'C
Cuts at: 0 169 818 3609
Size: 169 649 2791
Fragments arranged by size:
2791 649 169
SanDI GG'GwC_CC
Cuts at: 0 789 2538 3609
Size: 789 1749 1071
Fragments arranged by size:
1749 1071 789
SapI GCTCTTCn'nnn_
Cuts at: 0 7 2639 3609
Size: 7 2632 970
Fragments arranged by size:
2632 970 7
ScaI AGT'ACT
Cuts at: 0 400 3609
Size: 400 3209
SfcI C'TryA_G
Cuts at: 0 325 402 577 600 877 1325 2038 2230
Size: 325 77 175 23 277 448 713 192
Cuts at: 2230 2371 2867 3109 3370 3609
Size: 141 496 242 261 239
Fragments arranged by size:
713 496 448 325 277 261 242 239
192 175 141 77 23
SfoI GGC'GCC
Cuts at: 0 2465 3609
Size: 2465 1144
SgrAI Cr'CCGG_yG
Cuts at: 0 2460 3609
Size: 2460 1149
SmaI CCC'GGG
Cuts at: 0 382 595 1645 1963 3235 3609
Size: 382 213 1050 318 1272 374
Fragments arranged by size:
1272 1050 382 374 318 213
SmlI C'TyrA_G
Cuts at: 0 931 2947 3316 3609
Size: 931 2016 369 293
Fragments arranged by size:
2016 931 369 293
StuI AGG'CCT
Cuts at: 0 529 2080 3609
Size: 529 1551 1529
Fragments arranged by size:
1551 1529 529
StyI C'CwwG_G
Cuts at: 0 66 1454 2147 2636 3353 3609
Size: 66 1388 693 489 717 256
Fragments arranged by size:
1388 717 693 489 256 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2520 2619 2803 2850 2857 2861 3511 3538
Size: 2520 99 184 47 7 4 650 27
Cuts at: 3538 3609
Size: 71
Fragments arranged by size:
2520 650 184 99 71 47 27 7
4
TatI w'GTAC_w
Cuts at: 0 398 3609
Size: 398 3211
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1086 1118 1431 1463 2695 2727 2948 2980
Size: 1086 32 313 32 1232 32 221 32
Cuts at: 2980 3609
Size: 629
Fragments arranged by size:
1232 1086 629 313 221 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1017 2475 3609
Size: 1017 1458 1134
Fragments arranged by size:
1458 1134 1017
XhoI C'TCGA_G
Cuts at: 0 3316 3609
Size: 3316 293
XmaI C'CCGG_G
Cuts at: 0 380 593 1643 1961 3233 3609
Size: 380 213 1050 318 1272 376
Fragments arranged by size:
1272 1050 380 376 318 213
XmnI GAAnn'nnTTC
Cuts at: 0 2272 2713 3609
Size: 2272 441 896
Fragments arranged by size:
2272 896 441
ZraI GAC'GTC
Cuts at: 0 3432 3609
Size: 3432 177
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PciI PflMI PfoI PmlI PpiI PpuMI PsiI
PspOMI PspXI PstI PvuII SacI SanDI SapI ScaI
SfcI SfoI SgrAI SmaI SmlI StuI StyI TaqII
TatI TstI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AgeI AloI ApoI AscI AseI
AsiSI AvrII BaeI BclI BfrBI BplI BsaBI BsiWI
BsrGI BssHII BstBI BstZ17I ClaI CspCI EcoRI EcoRV
FspAI HindIII HpaI MfeI MluI NdeI NotI NruI
NsiI PacI PmeI PshAI PsrI PvuI RsrII SacII
SalI SbfI SexAI SfiI SgfI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI