Restriction map for
pFN21AE5810
(Linear) (Six-base) MAPSORT of: KIEE5810.seq Check: 5241 from: 1 to: 4365
KIEE5810.orf
With 182 enzymes: SgfI *
December 5, 2014 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1458 4365
Size: 1458 2907
AatII G_ACGT'C
Cuts at: 0 689 2173 4365
Size: 689 1484 2192
Fragments arranged by size:
2192 1484 689
AccI GT'mk_AC
Cuts at: 0 3455 4365
Size: 3455 910
Acc65I G'GTAC_C
Cuts at: 0 1460 4365
Size: 1460 2905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 942 967 1627 1840 1904 2843 2891
Size: 615 327 25 660 213 64 939 48
Cuts at: 2891 3190 3387 4365
Size: 299 197 978
Fragments arranged by size:
978 939 660 615 327 299 213 197
64 48 25
AflIII A'CryG_T
Cuts at: 0 1736 2265 2337 4076 4248 4365
Size: 1736 529 72 1739 172 117
Fragments arranged by size:
1739 1736 529 172 117 72
AleI CACnn'nnGTG
Cuts at: 0 2372 3368 3860 4365
Size: 2372 996 492 505
Fragments arranged by size:
2372 996 505 492
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2732 4365
Size: 2732 1633
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2473 2505 4365
Size: 2473 32 1860
Fragments arranged by size:
2473 1860 32
AlwNI CAG_nnn'CTG
Cuts at: 0 168 1215 2187 2845 3174 3682 4365
Size: 168 1047 972 658 329 508 683
Fragments arranged by size:
1047 972 683 658 508 329 168
ApaI G_GGCC'C
Cuts at: 0 1324 2320 4365
Size: 1324 996 2045
Fragments arranged by size:
2045 1324 996
ApaLI G'TGCA_C
Cuts at: 0 1471 2365 4365
Size: 1471 894 2000
Fragments arranged by size:
2000 1471 894
ApoI r'AATT_y
Cuts at: 0 2503 2813 3006 4365
Size: 2503 310 193 1359
Fragments arranged by size:
2503 1359 310 193
AvaI C'yCGr_G
Cuts at: 0 30 1769 2319 3581 3874 4365
Size: 30 1739 550 1262 293 491
Fragments arranged by size:
1739 1262 550 491 293 30
BamHI G'GATC_C
Cuts at: 0 3239 4365
Size: 3239 1126
BanI G'GyrC_C
Cuts at: 0 1460 3370 4226 4365
Size: 1460 1910 856 139
Fragments arranged by size:
1910 1460 856 139
BanII G_rGCy'C
Cuts at: 0 1324 1628 1946 2320 3574 4157 4365
Size: 1324 304 318 374 1254 583 208
Fragments arranged by size:
1324 1254 583 374 318 304 208
BbsI GAAGACnn'nnnn_
Cuts at: 0 428 1229 1404 4003 4037 4365
Size: 428 801 175 2599 34 328
Fragments arranged by size:
2599 801 428 328 175 34
BbvCI CC'TCA_GC
Cuts at: 0 1937 4365
Size: 1937 2428
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 592 626 4365
Size: 592 34 3739
Fragments arranged by size:
3739 592 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4107 4365
Size: 4107 258
BfrBI ATG'CAT
Cuts at: 0 609 4365
Size: 609 3756
BglII A'GATC_T
Cuts at: 0 368 753 4365
Size: 368 385 3612
Fragments arranged by size:
3612 385 368
BlpI GC'TnA_GC
Cuts at: 0 2110 4365
Size: 2110 2255
Bme1580I G_kGCm'C
Cuts at: 0 120 1324 1475 2320 2334 2369 3375 4231
Size: 120 1204 151 845 14 35 1006 856
Cuts at: 4231 4365
Size: 134
Fragments arranged by size:
1204 1006 856 845 151 134 120 35
14
BmgBI CAC'GTC
Cuts at: 0 2266 4365
Size: 2266 2099
BmrI ACTGGGnnnn_n'
Cuts at: 0 1236 1687 1978 2544 4365
Size: 1236 451 291 566 1821
Fragments arranged by size:
1821 1236 566 451 291
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 882 1341 2737 3663 4365
Size: 882 459 1396 926 702
Fragments arranged by size:
1396 926 882 702 459
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 869 1336 2152 2184 2567 2697 2824
Size: 365 504 467 816 32 383 130 127
Cuts at: 2824 3103 3151 3550 4118 4171 4291 4365
Size: 279 48 399 568 53 120 74
Fragments arranged by size:
816 568 504 467 399 383 365 279
130 127 120 74 53 48 32
Bpu10I CC'TnA_GC
Cuts at: 0 1937 4365
Size: 1937 2428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 291 1546 2967 4141 4365
Size: 205 86 1255 1421 1174 224
Fragments arranged by size:
1421 1255 1174 224 205 86
BsaI GGTCTCn'nnnn_
Cuts at: 0 382 2673 2918 3431 4365
Size: 382 2291 245 513 934
Fragments arranged by size:
2291 934 513 382 245
BsaAI yAC'GTr
Cuts at: 0 558 1737 4251 4365
Size: 558 1179 2514 114
Fragments arranged by size:
2514 1179 558 114
BsaHI Gr'CG_yC
Cuts at: 0 686 2170 4365
Size: 686 1484 2195
Fragments arranged by size:
2195 1484 686
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2255 2285 3227 3257 3343 3373 4365
Size: 2255 30 942 30 86 30 992
Fragments arranged by size:
2255 992 942 86 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 874 1120 1507 1954 2668 3235 3570 3775
Size: 874 246 387 447 714 567 335 205
Cuts at: 3775 3943 3946 4365
Size: 168 3 419
Fragments arranged by size:
874 714 567 447 419 387 335 246
205 168 3
BseYI C'CCAG_C
Cuts at: 0 312 1609 1999 2343 3651 3942 4108 4365
Size: 312 1297 390 344 1308 291 166 257
Fragments arranged by size:
1308 1297 390 344 312 291 257 166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 1121 1949 2402 2589 3668 3673 3884
Size: 357 764 828 453 187 1079 5 211
Cuts at: 3884 4365
Size: 481
Fragments arranged by size:
1079 828 764 481 453 357 211 187
5
BsiHKAI G_wGCw'C
Cuts at: 0 1169 1375 1475 1778 2273 2285 2369 2885
Size: 1169 206 100 303 495 12 84 516
Cuts at: 2885 3137 4157 4365
Size: 252 1020 208
Fragments arranged by size:
1169 1020 516 495 303 252 208 206
100 84 12
BsmI GAATG_Cn'
Cuts at: 0 177 821 1252 4365
Size: 177 644 431 3113
Fragments arranged by size:
3113 644 431 177
Bsp1286I G_dGCh'C
Cuts at: 0 120 1169 1324 1375 1475 1628 1778 1946
Size: 120 1049 155 51 100 153 150 168
Cuts at: 1946 2273 2285 2320 2334 2369 2885 3137 3375
Size: 327 12 35 14 35 516 252 238
Cuts at: 3375 3574 4157 4231 4365
Size: 199 583 74 134
Fragments arranged by size:
1049 583 516 327 252 238 199 168
155 153 150 134 120 100 74 51
35 35 14 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 780 1458 1472 2936 3848 4365
Size: 780 678 14 1464 912 517
Fragments arranged by size:
1464 912 780 678 517 14
BsrBI CCG'CTC
Cuts at: 0 1511 4365
Size: 1511 2854
BsrFI r'CCGG_y
Cuts at: 0 3548 3608 4365
Size: 3548 60 757
Fragments arranged by size:
3548 757 60
BsrGI T'GTAC_A
Cuts at: 0 2509 3230 4365
Size: 2509 721 1135
Fragments arranged by size:
2509 1135 721
BssSI C'ACGA_G
Cuts at: 0 9 1376 1438 2555 4365
Size: 9 1367 62 1117 1810
Fragments arranged by size:
1810 1367 1117 62 9
BstAPI GCAn_nnn'nTGC
Cuts at: 0 585 816 1012 1164 1215 2425 4365
Size: 585 231 196 152 51 1210 1940
Fragments arranged by size:
1940 1210 585 231 196 152 51
BstYI r'GATC_y
Cuts at: 0 368 753 939 3239 4365
Size: 368 385 186 2300 1126
Fragments arranged by size:
2300 1126 385 368 186
Bsu36I CC'TnA_GG
Cuts at: 0 3638 3937 4365
Size: 3638 299 428
Fragments arranged by size:
3638 428 299
BtgI C'CryG_G
Cuts at: 0 323 1386 2137 3363 4175 4365
Size: 323 1063 751 1226 812 190
Fragments arranged by size:
1226 1063 812 751 323 190
BtsI GCAGTG_nn'
Cuts at: 0 930 1283 2039 2285 3302 3389 3561 3685
Size: 930 353 756 246 1017 87 172 124
Cuts at: 3685 4365
Size: 680
Fragments arranged by size:
1017 930 756 680 353 246 172 124
87
DraIII CAC_nnn'GTG
Cuts at: 0 402 2425 4365
Size: 402 2023 1940
Fragments arranged by size:
2023 1940 402
DrdI GACnn_nn'nnGTC
Cuts at: 0 13 1279 4365
Size: 13 1266 3086
Fragments arranged by size:
3086 1266 13
EaeI y'GGCC_r
Cuts at: 0 1116 1538 1553 2360 3027 3114 3467 3520
Size: 1116 422 15 807 667 87 353 53
Cuts at: 3520 4037 4365
Size: 517 328
Fragments arranged by size:
1116 807 667 517 422 353 328 87
53 15
EarI CTCTTCn'nnn_
Cuts at: 0 634 943 1646 1816 1880 2248 3883 4043
Size: 634 309 703 170 64 368 1635 160
Cuts at: 4043 4105 4365
Size: 62 260
Fragments arranged by size:
1635 703 634 368 309 260 170 160
64 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 15 3238 4365
Size: 15 3223 1127
Fragments arranged by size:
3223 1127 15
EcoICRI GAG'CTC
Cuts at: 0 4155 4365
Size: 4155 210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 615 869 942 967 1336 1627 1840
Size: 365 250 254 73 25 369 291 213
Cuts at: 1840 1904 2152 2184 2567 2697 2824 2843 2891
Size: 64 248 32 383 130 127 19 48
Cuts at: 2891 3103 3151 3190 3387 3550 4118 4171 4291
Size: 212 48 39 197 163 568 53 120
Cuts at: 4291 4365
Size: 74
Fragments arranged by size:
568 383 369 365 291 254 250 248
213 212 197 163 130 127 120 74
73 64 53 48 48 39 32 25
19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3379 3844 4345 4365
Size: 3379 465 501 20
Fragments arranged by size:
3379 501 465 20
EcoO109I rG'GnC_Cy
Cuts at: 0 15 39 59 165 1320 1321 1404 2227
Size: 15 24 20 106 1155 1 83 823
Cuts at: 2227 2316 2410 3408 4365
Size: 89 94 998 957
Fragments arranged by size:
1155 998 957 823 106 94 89 83
24 20 15 1
EcoRV GAT'ATC
Cuts at: 0 2096 3899 4365
Size: 2096 1803 466
Fragments arranged by size:
2096 1803 466
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 972 1380 4216 4365
Size: 972 408 2836 149
Fragments arranged by size:
2836 972 408 149
HaeII r_GCGC'y
Cuts at: 0 4301 4365
Size: 4301 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 289 321 611 643 969 1001 1041 1073
Size: 289 32 290 32 326 32 40 32
Cuts at: 1073 1516 1548 1585 1588 1617 1620 2002 2034
Size: 443 32 37 3 29 3 382 32
Cuts at: 2034 2211 2243 2255 2287 2368 2400 3492 3524
Size: 177 32 12 32 81 32 1092 32
Cuts at: 3524 3616 3648 3658 3690 4365
Size: 92 32 10 32 675
Fragments arranged by size:
1092 675 443 382 326 290 289 177
92 81 40 37 32 32 32 32
32 32 32 32 32 32 32 32
29 12 10 3 3
HincII GTy'rAC
Cuts at: 0 2955 3336 3456 3923 4365
Size: 2955 381 120 467 442
Fragments arranged by size:
2955 467 442 381 120
KpnI G_GTAC'C
Cuts at: 0 1464 4365
Size: 1464 2901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 4365
Size: 29 4336
MscI TGG'CCA
Cuts at: 0 1118 1540 3469 4365
Size: 1118 422 1929 896
Fragments arranged by size:
1929 1118 896 422
MslI CAynn'nnrTG
Cuts at: 0 1666 1702 2152 2372 3368 3860 4365
Size: 1666 36 450 220 996 492 505
Fragments arranged by size:
1666 996 505 492 450 220 36
MspA1I CmG'CkG
Cuts at: 0 139 385 1215 1269 1609 1963 2301 2573
Size: 139 246 830 54 340 354 338 272
Cuts at: 2573 3576 3679 4138 4365
Size: 1003 103 459 227
Fragments arranged by size:
1003 830 459 354 340 338 272 246
227 139 103 54
NaeI GCC'GGC
Cuts at: 0 3610 4365
Size: 3610 755
NcoI C'CATG_G
Cuts at: 0 1386 4365
Size: 1386 2979
NdeI CA'TA_TG
Cuts at: 0 1341 4365
Size: 1341 3024
NgoMIV G'CCGG_C
Cuts at: 0 3608 4365
Size: 3608 757
NsiI A_TGCA'T
Cuts at: 0 611 4365
Size: 611 3754
NspI r_CATG'y
Cuts at: 0 609 613 682 2341 4066 4080 4365
Size: 609 4 69 1659 1725 14 285
Fragments arranged by size:
1725 1659 609 285 69 14 4
PasI CC'CwG_GG
Cuts at: 0 43 1908 1947 2231 4365
Size: 43 1865 39 284 2134
Fragments arranged by size:
2134 1865 284 43 39
PciI A'CATG_T
Cuts at: 0 2337 4076 4365
Size: 2337 1739 289
Fragments arranged by size:
2337 1739 289
PfoI T'CCnGG_A
Cuts at: 0 2674 4365
Size: 2674 1691
PmlI CAC'GTG
Cuts at: 0 4251 4365
Size: 4251 114
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 4365
Size: 13 32 4320
Fragments arranged by size:
4320 32 13
PpuMI rG'GwC_Cy
Cuts at: 0 15 39 165 2227 2410 4365
Size: 15 24 126 2062 183 1955
Fragments arranged by size:
2062 1955 183 126 24 15
PspOMI G'GGCC_C
Cuts at: 0 1320 2316 4365
Size: 1320 996 2049
Fragments arranged by size:
2049 1320 996
PspXI vC'TCGA_Gb
Cuts at: 0 1769 3874 4365
Size: 1769 2105 491
Fragments arranged by size:
2105 1769 491
PstI C_TGCA'G
Cuts at: 0 812 1396 1469 2280 2703 2933 3092 3593
Size: 812 584 73 811 423 230 159 501
Cuts at: 3593 4214 4365
Size: 621 151
Fragments arranged by size:
812 811 621 584 501 423 230 159
151 73
PvuII CAG'CTG
Cuts at: 0 139 1215 1269 1609 1963 2301 2573 3679
Size: 139 1076 54 340 354 338 272 1106
Cuts at: 3679 4138 4365
Size: 459 227
Fragments arranged by size:
1106 1076 459 354 340 338 272 227
139 54
SacI G_AGCT'C
Cuts at: 0 4157 4365
Size: 4157 208
SalI G'TCGA_C
Cuts at: 0 3454 4365
Size: 3454 911
SanDI GG'GwC_CC
Cuts at: 0 15 2227 4365
Size: 15 2212 2138
Fragments arranged by size:
2212 2138 15
SapI GCTCTTCn'nnn_
Cuts at: 0 634 1646 4105 4365
Size: 634 1012 2459 260
Fragments arranged by size:
2459 1012 634 260
SbfI CC_TGCA'GG
Cuts at: 0 1469 2933 4365
Size: 1469 1464 1432
Fragments arranged by size:
1469 1464 1432
SexAI A'CCwGG_T
Cuts at: 0 2421 4365
Size: 2421 1944
SfcI C'TryA_G
Cuts at: 0 808 1392 1465 1571 2276 2469 2699 2929
Size: 808 584 73 106 705 193 230 230
Cuts at: 2929 3088 3589 4210 4365
Size: 159 501 621 155
Fragments arranged by size:
808 705 621 584 501 230 230 193
159 155 106 73
SmaI CCC'GGG
Cuts at: 0 3583 4365
Size: 3583 782
SmlI C'TyrA_G
Cuts at: 0 184 306 1561 1769 2982 3874 4156 4365
Size: 184 122 1255 208 1213 892 282 209
Fragments arranged by size:
1255 1213 892 282 209 208 184 122
SphI G_CATG'C
Cuts at: 0 613 682 4365
Size: 613 69 3683
Fragments arranged by size:
3683 613 69
StuI AGG'CCT
Cuts at: 0 253 1360 1728 1870 2588 4054 4365
Size: 253 1107 368 142 718 1466 311
Fragments arranged by size:
1466 1107 718 368 311 253 142
StyI C'CwwG_G
Cuts at: 0 1386 4365
Size: 1386 2979
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1218 1499 2362 3672 4365
Size: 1218 281 863 1310 693
Fragments arranged by size:
1310 1218 863 693 281
TatI w'GTAC_w
Cuts at: 0 804 852 1850 2509 3230 4079 4365
Size: 804 48 998 659 721 849 286
Fragments arranged by size:
998 849 804 721 659 286 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2244 2276 3109 3141 4365
Size: 2244 32 833 32 1224
Fragments arranged by size:
2244 1224 833 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1548 4365
Size: 1548 2817
XhoI C'TCGA_G
Cuts at: 0 1769 3874 4365
Size: 1769 2105 491
Fragments arranged by size:
2105 1769 491
XmaI C'CCGG_G
Cuts at: 0 3581 4365
Size: 3581 784
XmnI GAAnn'nnTTC
Cuts at: 0 952 2771 4365
Size: 952 1819 1594
Fragments arranged by size:
1819 1594 952
ZraI GAC'GTC
Cuts at: 0 687 2171 4365
Size: 687 1484 2194
Fragments arranged by size:
2194 1484 687
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstYI
Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I
HincII KpnI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NsiI NspI PasI PciI PfoI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuII SacI SalI
SanDI SapI SbfI SexAI SfcI SmaI SmlI SphI
StuI StyI TaqII TatI TstI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AhdI AscI AseI AsiSI
AvrII BaeI BbeI BclI BglI BmtI BsaBI BsaWI
BsiEI BsiWI BsmBI BspEI BspHI BsrDI BssHII BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI EagI
EcoRI FseI FspI FspAI HindIII HpaI KasI MfeI
MluI NarI NheI NotI NruI PacI PflMI PmeI
PshAI PsiI PsrI PvuI RsrII SacII ScaI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI