(Linear) (Six-base) MAPSORT of: KIEE5807.seq Check: 3824 from: 1 to: 4218 KIEE5807.orf With 182 enzymes: SgfI * December 5, 2014 13:11 .. Acc65I G'GTAC_C Cuts at: 0 1341 4218 Size: 1341 2877 AclI AA'CG_TT Cuts at: 0 1375 1533 3599 4218 Size: 1375 158 2066 619 Fragments arranged by size: 2066 1375 619 158 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 107 485 1048 1207 1261 1523 2645 2977 Size: 107 378 563 159 54 262 1122 332 Cuts at: 2977 3566 4218 Size: 589 652 Fragments arranged by size: 1122 652 589 563 378 332 262 159 107 54 AflII C'TTAA_G Cuts at: 0 609 4218 Size: 609 3609 AflIII A'CryG_T Cuts at: 0 69 4218 Size: 69 4149 AleI CACnn'nnGTG Cuts at: 0 424 3847 4218 Size: 424 3423 371 Fragments arranged by size: 3423 424 371 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 784 1271 4218 Size: 784 487 2947 Fragments arranged by size: 2947 784 487 AlwNI CAG_nnn'CTG Cuts at: 0 312 999 1590 4218 Size: 312 687 591 2628 Fragments arranged by size: 2628 687 591 312 ApaI G_GGCC'C Cuts at: 0 58 4218 Size: 58 4160 ApaLI G'TGCA_C Cuts at: 0 3794 4218 Size: 3794 424 ApoI r'AATT_y Cuts at: 0 370 491 523 619 640 2485 3120 3210 Size: 370 121 32 96 21 1845 635 90 Cuts at: 3210 3355 3460 3477 4218 Size: 145 105 17 741 Fragments arranged by size: 1845 741 635 370 145 121 105 96 90 32 21 17 AseI AT'TA_AT Cuts at: 0 2152 4218 Size: 2152 2066 AvaI C'yCGr_G Cuts at: 0 114 4218 Size: 114 4104 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 3619 3652 4218 Size: 3619 33 566 Fragments arranged by size: 3619 566 33 BanI G'GyrC_C Cuts at: 0 1341 4218 Size: 1341 2877 BanII G_rGCy'C Cuts at: 0 58 1561 4218 Size: 58 1503 2657 Fragments arranged by size: 2657 1503 58 BbsI GAAGACnn'nnnn_ Cuts at: 0 863 2735 3352 3808 4218 Size: 863 1872 617 456 410 Fragments arranged by size: 1872 863 617 456 410 BbvCI CC'TCA_GC Cuts at: 0 238 2578 4218 Size: 238 2340 1640 Fragments arranged by size: 2340 1640 238 BciVI GTATCCnnnnn_n' Cuts at: 0 3084 3227 4218 Size: 3084 143 991 Fragments arranged by size: 3084 991 143 BclI T'GATC_A Cuts at: 0 2372 4218 Size: 2372 1846 BfrBI ATG'CAT Cuts at: 0 2347 3396 4218 Size: 2347 1049 822 Fragments arranged by size: 2347 1049 822 BglII A'GATC_T Cuts at: 0 414 1999 2443 4218 Size: 414 1585 444 1775 Fragments arranged by size: 1775 1585 444 414 BlpI GC'TnA_GC Cuts at: 0 325 4218 Size: 325 3893 Bme1580I G_kGCm'C Cuts at: 0 58 3798 4218 Size: 58 3740 420 Fragments arranged by size: 3740 420 58 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 291 2048 4218 Size: 291 1757 2170 Fragments arranged by size: 2170 1757 291 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 334 382 748 802 2018 2320 4218 Size: 334 48 366 54 1216 302 1898 Fragments arranged by size: 1898 1216 366 334 302 54 48 Bpu10I CC'TnA_GC Cuts at: 0 238 2578 4218 Size: 238 2340 1640 Fragments arranged by size: 2340 1640 238 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1295 3658 4218 Size: 1295 2363 560 Fragments arranged by size: 2363 1295 560 BsaI GGTCTCn'nnnn_ Cuts at: 0 2203 4218 Size: 2203 2015 BsaAI yAC'GTr Cuts at: 0 70 4218 Size: 70 4148 BsaHI Gr'CG_yC Cuts at: 0 3938 4218 Size: 3938 280 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 74 104 4218 Size: 74 30 4114 Fragments arranged by size: 4114 74 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 226 298 355 745 2040 2337 3046 3667 Size: 226 72 57 390 1295 297 709 621 Cuts at: 3667 4218 Size: 551 Fragments arranged by size: 1295 709 621 551 390 297 226 72 57 BseYI C'CCAG_C Cuts at: 0 39 51 1037 2591 3133 4218 Size: 39 12 986 1554 542 1085 Fragments arranged by size: 1554 1085 986 542 39 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 744 4218 Size: 744 3474 BsiHKAI G_wGCw'C Cuts at: 0 1296 1561 3798 4218 Size: 1296 265 2237 420 Fragments arranged by size: 2237 1296 420 265 BsmI GAATG_Cn' Cuts at: 0 2820 3003 3312 4218 Size: 2820 183 309 906 Fragments arranged by size: 2820 906 309 183 BsmBI CGTCTCn'nnnn_ Cuts at: 0 400 4218 Size: 400 3818 Bsp1286I G_dGCh'C Cuts at: 0 58 1296 1561 3798 4218 Size: 58 1238 265 2237 420 Fragments arranged by size: 2237 1238 420 265 58 BspHI T'CATG_A Cuts at: 0 1490 2805 4218 Size: 1490 1315 1413 Fragments arranged by size: 1490 1413 1315 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 776 4218 Size: 776 3442 BsrDI GCAATG_nn' Cuts at: 0 104 2568 3725 3766 3805 4218 Size: 104 2464 1157 41 39 413 Fragments arranged by size: 2464 1157 413 104 41 39 BsrFI r'CCGG_y Cuts at: 0 124 194 4218 Size: 124 70 4024 Fragments arranged by size: 4024 124 70 BsrGI T'GTAC_A Cuts at: 0 684 4091 4218 Size: 684 3407 127 Fragments arranged by size: 3407 684 127 BssHII G'CGCG_C Cuts at: 0 242 4218 Size: 242 3976 BstAPI GCAn_nnn'nTGC Cuts at: 0 999 4218 Size: 999 3219 BstEII G'GTnAC_C Cuts at: 0 294 4218 Size: 294 3924 BstYI r'GATC_y Cuts at: 0 414 1794 1999 2443 3669 4218 Size: 414 1380 205 444 1226 549 Fragments arranged by size: 1380 1226 549 444 414 205 Bsu36I CC'TnA_GG Cuts at: 0 2320 4218 Size: 2320 1898 BtgI C'CryG_G Cuts at: 0 248 3229 4218 Size: 248 2981 989 Fragments arranged by size: 2981 989 248 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 304 1333 4218 Size: 304 1029 2885 Fragments arranged by size: 2885 1029 304 BtsI GCAGTG_nn' Cuts at: 0 1764 2622 4218 Size: 1764 858 1596 Fragments arranged by size: 1764 1596 858 DraI TTT'AAA Cuts at: 0 645 2490 2771 4218 Size: 645 1845 281 1447 Fragments arranged by size: 1845 1447 645 281 EaeI y'GGCC_r Cuts at: 0 2426 3860 4218 Size: 2426 1434 358 Fragments arranged by size: 2426 1434 358 EarI CTCTTCn'nnn_ Cuts at: 0 314 692 1304 1571 1974 2360 3827 4218 Size: 314 378 612 267 403 386 1467 391 Fragments arranged by size: 1467 612 403 391 386 378 314 267 EcoICRI GAG'CTC Cuts at: 0 1559 4218 Size: 1559 2659 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 107 334 382 485 748 802 1048 1207 Size: 107 227 48 103 263 54 246 159 Cuts at: 1207 1261 1523 2018 2320 2645 2977 3566 4218 Size: 54 262 495 302 325 332 589 652 Fragments arranged by size: 652 589 495 332 325 302 263 262 246 227 159 107 103 54 54 48 EcoNI CCTnn'n_nnAGG Cuts at: 0 337 355 2581 4218 Size: 337 18 2226 1637 Fragments arranged by size: 2226 1637 337 18 EcoO109I rG'GnC_Cy Cuts at: 0 55 62 202 3783 4218 Size: 55 7 140 3581 435 Fragments arranged by size: 3581 435 140 55 7 EcoRI G'AATT_C Cuts at: 0 619 4218 Size: 619 3599 EcoRV GAT'ATC Cuts at: 0 3407 4218 Size: 3407 811 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1291 4135 4218 Size: 1291 2844 83 Fragments arranged by size: 2844 1291 83 FspI TGC'GCA Cuts at: 0 103 4218 Size: 103 4115 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1779 1811 2104 2136 3166 3198 3697 3729 Size: 1779 32 293 32 1030 32 499 32 Cuts at: 3729 4218 Size: 489 Fragments arranged by size: 1779 1030 499 489 293 32 32 32 32 HincII GTy'rAC Cuts at: 0 2892 4218 Size: 2892 1326 HindIII A'AGCT_T Cuts at: 0 1365 3676 4218 Size: 1365 2311 542 Fragments arranged by size: 2311 1365 542 KpnI G_GTAC'C Cuts at: 0 1345 4218 Size: 1345 2873 MfeI C'AATT_G Cuts at: 0 3286 3572 4218 Size: 3286 286 646 Fragments arranged by size: 3286 646 286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2039 2069 2746 3581 4218 Size: 2039 30 677 835 637 Fragments arranged by size: 2039 835 677 637 30 MscI TGG'CCA Cuts at: 0 2428 3862 4218 Size: 2428 1434 356 Fragments arranged by size: 2428 1434 356 MslI CAynn'nnrTG Cuts at: 0 424 2437 3847 4201 4218 Size: 424 2013 1410 354 17 Fragments arranged by size: 2013 1410 424 354 17 MspA1I CmG'CkG Cuts at: 0 51 309 758 1123 4218 Size: 51 258 449 365 3095 Fragments arranged by size: 3095 449 365 258 51 NaeI GCC'GGC Cuts at: 0 126 196 4218 Size: 126 70 4022 Fragments arranged by size: 4022 126 70 NcoI C'CATG_G Cuts at: 0 248 4218 Size: 248 3970 NgoMIV G'CCGG_C Cuts at: 0 124 194 4218 Size: 124 70 4024 Fragments arranged by size: 4024 124 70 NsiI A_TGCA'T Cuts at: 0 2349 3398 4218 Size: 2349 1049 820 Fragments arranged by size: 2349 1049 820 PflMI CCAn_nnn'nTGG Cuts at: 0 73 3972 4218 Size: 73 3899 246 Fragments arranged by size: 3899 246 73 PmlI CAC'GTG Cuts at: 0 70 4218 Size: 70 4148 PpuMI rG'GwC_Cy Cuts at: 0 62 3783 4218 Size: 62 3721 435 Fragments arranged by size: 3721 435 62 PshAI GACnn'nnGTC Cuts at: 0 2308 4218 Size: 2308 1910 PsiI TTA'TAA Cuts at: 0 718 4218 Size: 718 3500 PspOMI G'GGCC_C Cuts at: 0 54 4218 Size: 54 4164 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1904 1936 4218 Size: 1904 32 2282 Fragments arranged by size: 2282 1904 32 PstI C_TGCA'G Cuts at: 0 995 1004 4218 Size: 995 9 3214 Fragments arranged by size: 3214 995 9 PvuII CAG'CTG Cuts at: 0 51 309 1123 4218 Size: 51 258 814 3095 Fragments arranged by size: 3095 814 258 51 SacI G_AGCT'C Cuts at: 0 1561 4218 Size: 1561 2657 SapI GCTCTTCn'nnn_ Cuts at: 0 314 692 1571 2360 4218 Size: 314 378 879 789 1858 Fragments arranged by size: 1858 879 789 378 314 ScaI AGT'ACT Cuts at: 0 545 1094 2031 4151 4218 Size: 545 549 937 2120 67 Fragments arranged by size: 2120 937 549 545 67 SfcI C'TryA_G Cuts at: 0 991 1000 1582 1696 1716 4218 Size: 991 9 582 114 20 2502 Fragments arranged by size: 2502 991 582 114 20 9 SmlI C'TyrA_G Cuts at: 0 609 1310 3673 4218 Size: 609 701 2363 545 Fragments arranged by size: 2363 701 609 545 SpeI A'CTAG_T Cuts at: 0 711 4179 4218 Size: 711 3468 39 Fragments arranged by size: 3468 711 39 SspI AAT'ATT Cuts at: 0 2940 4019 4218 Size: 2940 1079 199 Fragments arranged by size: 2940 1079 199 StuI AGG'CCT Cuts at: 0 112 4218 Size: 112 4106 StyI C'CwwG_G Cuts at: 0 248 2477 3971 4218 Size: 248 2229 1494 247 Fragments arranged by size: 2229 1494 248 247 SwaI ATTT'AAAT Cuts at: 0 2771 4218 Size: 2771 1447 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 159 163 4218 Size: 159 4 4055 Fragments arranged by size: 4055 159 4 TatI w'GTAC_w Cuts at: 0 90 543 684 1024 1092 1920 2029 2757 Size: 90 453 141 340 68 828 109 728 Cuts at: 2757 2903 2988 4091 4149 4218 Size: 146 85 1103 58 69 Fragments arranged by size: 1103 828 728 453 340 146 141 109 90 85 69 68 58 XbaI T'CTAG_A Cuts at: 0 486 4218 Size: 486 3732 XmnI GAAnn'nnTTC Cuts at: 0 619 1112 1274 4218 Size: 619 493 162 2944 Fragments arranged by size: 2944 619 493 162 Enzymes that do cut: Acc65I AclI AcuI AflII AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI BaeI BanI BanII BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI PflMI PmlI PpuMI PshAI PsiI PspOMI PsrI PstI PvuII SacI SapI ScaI SfcI SmlI SpeI SspI StuI StyI SwaI TaqII TatI XbaI XmnI Enzymes that do not cut: AarI AatII AccI AfeI AgeI AhdI AloI AscI AsiSI AvrII BamHI BbeI BcgI BglI BmgBI BmrI BmtI BsaBI BsaWI BsiEI BsiWI BspEI BsrBI BssSI BstBI BstXI BstZ17I ClaI CspCI DraIII DrdI EagI EciI FseI FspAI HaeII HpaI KasI MluI NarI NdeI NheI NotI NruI NspI PacI PasI PciI PfoI PmeI PpiI PspXI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI TstI Tth111I XcmI XhoI XmaI ZraI