Restriction map for
pFN21AE5794
(Linear) (Six-base) MAPSORT of: KIEE5794.seq Check: 3102 from: 1 to: 2067
KIEE5794.orf
With 182 enzymes: SgfI *
December 5, 2014 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 733 2067
Size: 733 1334
AccI GT'mk_AC
Cuts at: 0 718 2067
Size: 718 1349
AclI AA'CG_TT
Cuts at: 0 325 2067
Size: 325 1742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 1465 1528 2067
Size: 566 899 63 539
Fragments arranged by size:
899 566 539 63
AgeI A'CCGG_T
Cuts at: 0 214 2067
Size: 214 1853
AleI CACnn'nnGTG
Cuts at: 0 1528 2067
Size: 1528 539
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1276 1630 2067
Size: 1276 354 437
Fragments arranged by size:
1276 437 354
AlwNI CAG_nnn'CTG
Cuts at: 0 657 799 894 2067
Size: 657 142 95 1173
Fragments arranged by size:
1173 657 142 95
ApoI r'AATT_y
Cuts at: 0 831 2067
Size: 831 1236
AvaI C'yCGr_G
Cuts at: 0 137 1621 1811 1950 2067
Size: 137 1484 190 139 117
Fragments arranged by size:
1484 190 139 137 117
BamHI G'GATC_C
Cuts at: 0 1840 2067
Size: 1840 227
BanI G'GyrC_C
Cuts at: 0 745 897 1260 1414 2067
Size: 745 152 363 154 653
Fragments arranged by size:
745 653 363 154 152
BanII G_rGCy'C
Cuts at: 0 1300 1894 2067
Size: 1300 594 173
Fragments arranged by size:
1300 594 173
BbeI G_GCGC'C
Cuts at: 0 1418 2067
Size: 1418 649
BbsI GAAGACnn'nnnn_
Cuts at: 0 332 2067
Size: 332 1735
BbvCI CC'TCA_GC
Cuts at: 0 203 2067
Size: 203 1864
BlpI GC'TnA_GC
Cuts at: 0 389 2067
Size: 389 1678
Bme1580I G_kGCm'C
Cuts at: 0 1265 1619 2067
Size: 1265 354 448
Fragments arranged by size:
1265 448 354
BmgBI CAC'GTC
Cuts at: 0 1329 2067
Size: 1329 738
BmrI ACTGGGnnnn_n'
Cuts at: 0 748 2067
Size: 748 1319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 653 722 1447 2067
Size: 99 554 69 725 620
Fragments arranged by size:
725 620 554 99 69
Bpu10I CC'TnA_GC
Cuts at: 0 203 1931 2067
Size: 203 1728 136
Fragments arranged by size:
1728 203 136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1024 2067
Size: 1024 1043
BsaI GGTCTCn'nnnn_
Cuts at: 0 44 428 810 2067
Size: 44 384 382 1257
Fragments arranged by size:
1257 384 382 44
BsaHI Gr'CG_yC
Cuts at: 0 1415 2067
Size: 1415 652
BsaWI w'CCGG_w
Cuts at: 0 214 1924 2067
Size: 214 1710 143
Fragments arranged by size:
1710 214 143
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 38 68 97 127 164 194 763 793
Size: 38 30 29 30 37 30 569 30
Cuts at: 793 2067
Size: 1274
Fragments arranged by size:
1274 569 38 37 30 30 30 30
29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 548 1374 1996 2067
Size: 548 826 622 71
Fragments arranged by size:
826 622 548 71
BseYI C'CCAG_C
Cuts at: 0 154 712 1078 1800 1913 2067
Size: 154 558 366 722 113 154
Fragments arranged by size:
722 558 366 154 154 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1351 2067
Size: 1351 716
BsiEI CG_ry'CG
Cuts at: 0 1529 2067
Size: 1529 538
BsiHKAI G_wGCw'C
Cuts at: 0 1628 1788 1932 2067
Size: 1628 160 144 135
Fragments arranged by size:
1628 160 144 135
BsmI GAATG_Cn'
Cuts at: 0 34 2067
Size: 34 2033
Bsp1286I G_dGCh'C
Cuts at: 0 1265 1300 1619 1628 1788 1894 1932 2067
Size: 1265 35 319 9 160 106 38 135
Fragments arranged by size:
1265 319 160 135 106 38 35 9
BspHI T'CATG_A
Cuts at: 0 12 1406 2067
Size: 12 1394 661
Fragments arranged by size:
1394 661 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 717 733 2067
Size: 717 16 1334
Fragments arranged by size:
1334 717 16
BsrBI CCG'CTC
Cuts at: 0 1499 2067
Size: 1499 568
BsrDI GCAATG_nn'
Cuts at: 0 1241 2067
Size: 1241 826
BsrFI r'CCGG_y
Cuts at: 0 214 1878 2067
Size: 214 1664 189
Fragments arranged by size:
1664 214 189
BssSI C'ACGA_G
Cuts at: 0 821 2067
Size: 821 1246
BstAPI GCAn_nnn'nTGC
Cuts at: 0 799 1993 2067
Size: 799 1194 74
Fragments arranged by size:
1194 799 74
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1236 1555 2067
Size: 1236 319 512
Fragments arranged by size:
1236 512 319
BstYI r'GATC_y
Cuts at: 0 1304 1840 2067
Size: 1304 536 227
Fragments arranged by size:
1304 536 227
BstZ17I GTA'TAC
Cuts at: 0 719 2067
Size: 719 1348
Bsu36I CC'TnA_GG
Cuts at: 0 767 2067
Size: 767 1300
BtgI C'CryG_G
Cuts at: 0 952 1176 2067
Size: 952 224 891
Fragments arranged by size:
952 891 224
BtsI GCAGTG_nn'
Cuts at: 0 1491 2067
Size: 1491 576
DraIII CAC_nnn'GTG
Cuts at: 0 522 2067
Size: 522 1545
DrdI GACnn_nn'nnGTC
Cuts at: 0 1006 2067
Size: 1006 1061
EaeI y'GGCC_r
Cuts at: 0 1644 1942 2067
Size: 1644 298 125
Fragments arranged by size:
1644 298 125
EarI CTCTTCn'nnn_
Cuts at: 0 1693 1864 2067
Size: 1693 171 203
Fragments arranged by size:
1693 203 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1689 2067
Size: 1689 378
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 566 653 722 1447 1465 1528 2067
Size: 99 467 87 69 725 18 63 539
Fragments arranged by size:
725 539 467 99 87 69 63 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 740 2067
Size: 740 1327
EcoO109I rG'GnC_Cy
Cuts at: 0 986 1458 1804 2067
Size: 986 472 346 263
Fragments arranged by size:
986 472 346 263
FspI TGC'GCA
Cuts at: 0 1483 2067
Size: 1483 584
HaeII r_GCGC'y
Cuts at: 0 1028 1418 2067
Size: 1028 390 649
Fragments arranged by size:
1028 649 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 164 196 763 795 2067
Size: 164 32 567 32 1272
Fragments arranged by size:
1272 567 164 32 32
KasI G'GCGC_C
Cuts at: 0 1414 2067
Size: 1414 653
MfeI C'AATT_G
Cuts at: 0 628 2067
Size: 628 1439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 2067
Size: 655 1412
MscI TGG'CCA
Cuts at: 0 1646 1944 2067
Size: 1646 298 123
Fragments arranged by size:
1646 298 123
MslI CAynn'nnrTG
Cuts at: 0 1118 1528 1612 2067
Size: 1118 410 84 455
Fragments arranged by size:
1118 455 410 84
MspA1I CmG'CkG
Cuts at: 0 92 657 796 1014 1428 1781 1800 2067
Size: 92 565 139 218 414 353 19 267
Fragments arranged by size:
565 414 353 267 218 139 92 19
NaeI GCC'GGC
Cuts at: 0 1880 2067
Size: 1880 187
NarI GG'CG_CC
Cuts at: 0 1415 2067
Size: 1415 652
NdeI CA'TA_TG
Cuts at: 0 528 2067
Size: 528 1539
NgoMIV G'CCGG_C
Cuts at: 0 1878 2067
Size: 1878 189
NspI r_CATG'y
Cuts at: 0 243 1974 2067
Size: 243 1731 93
Fragments arranged by size:
1731 243 93
PasI CC'CwG_GG
Cuts at: 0 1669 2067
Size: 1669 398
PflMI CCAn_nnn'nTGG
Cuts at: 0 894 1017 2067
Size: 894 123 1050
Fragments arranged by size:
1050 894 123
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1747 1779 2067
Size: 1747 32 288
Fragments arranged by size:
1747 288 32
PpuMI rG'GwC_Cy
Cuts at: 0 986 1458 1804 2067
Size: 986 472 346 263
Fragments arranged by size:
986 472 346 263
PstI C_TGCA'G
Cuts at: 0 413 1152 2067
Size: 413 739 915
Fragments arranged by size:
915 739 413
PvuII CAG'CTG
Cuts at: 0 92 796 1428 1781 1800 2067
Size: 92 704 632 353 19 267
Fragments arranged by size:
704 632 353 267 92 19
SanDI GG'GwC_CC
Cuts at: 0 986 1804 2067
Size: 986 818 263
Fragments arranged by size:
986 818 263
SapI GCTCTTCn'nnn_
Cuts at: 0 1864 2067
Size: 1864 203
ScaI AGT'ACT
Cuts at: 0 648 2067
Size: 648 1419
SexAI A'CCwGG_T
Cuts at: 0 1256 2067
Size: 1256 811
SfcI C'TryA_G
Cuts at: 0 409 1148 1711 2067
Size: 409 739 563 356
Fragments arranged by size:
739 563 409 356
SfoI GGC'GCC
Cuts at: 0 1416 2067
Size: 1416 651
SmlI C'TyrA_G
Cuts at: 0 1003 2067
Size: 1003 1064
SphI G_CATG'C
Cuts at: 0 243 1974 2067
Size: 243 1731 93
Fragments arranged by size:
1731 243 93
StuI AGG'CCT
Cuts at: 0 1283 1821 1936 2067
Size: 1283 538 115 131
Fragments arranged by size:
1283 538 131 115
TatI w'GTAC_w
Cuts at: 0 646 2067
Size: 646 1421
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 97 129 443 475 1381 1413 2067
Size: 97 32 314 32 906 32 654
Fragments arranged by size:
906 654 314 97 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 72 183 1018 1254 1425 2067
Size: 72 111 835 236 171 642
Fragments arranged by size:
835 642 236 171 111 72
XmnI GAAnn'nnTTC
Cuts at: 0 32 2067
Size: 32 2035
Enzymes that do cut:
AarI AccI AclI AcuI AgeI AleI AlfI AlwNI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraIII
DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I KasI MfeI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PasI PflMI PpiI
PpuMI PstI PvuII SanDI SapI ScaI SexAI SfcI
SfoI SmlI SphI StuI TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AflIII AhdI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BcgI BciVI
BclI BfrBI BglI BglII BmtI BplI BsaAI BsaBI
BsiWI BsmBI BspEI BsrGI BssHII BstBI BstEII BtgZI
ClaI CspCI DraI EagI EcoICRI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KpnI MluI NcoI
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SbfI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StyI SwaI TaqII Tth111I
XbaI XhoI XmaI ZraI