Restriction map for
pFN21AE5784
(Linear) (Six-base) MAPSORT of: KIEE5784.seq Check: 2741 from: 1 to: 2094
KIEE5784.orf
With 182 enzymes: SgfI *
December 5, 2014 13:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 691 1150 2094
Size: 55 636 459 944
Fragments arranged by size:
944 636 459 55
AfeI AGC'GCT
Cuts at: 0 1787 2094
Size: 1787 307
AflII C'TTAA_G
Cuts at: 0 1414 2094
Size: 1414 680
AflIII A'CryG_T
Cuts at: 0 488 1628 2094
Size: 488 1140 466
Fragments arranged by size:
1140 488 466
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 769 2094
Size: 769 1325
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 795 827 2094
Size: 795 32 1267
Fragments arranged by size:
1267 795 32
AlwNI CAG_nnn'CTG
Cuts at: 0 861 1020 1173 1231 1747 1815 2094
Size: 861 159 153 58 516 68 279
Fragments arranged by size:
861 516 279 159 153 68 58
ApoI r'AATT_y
Cuts at: 0 149 259 2094
Size: 149 110 1835
Fragments arranged by size:
1835 149 110
AvaI C'yCGr_G
Cuts at: 0 2077 2094
Size: 2077 17
AvrII C'CTAG_G
Cuts at: 0 394 2094
Size: 394 1700
BanI G'GyrC_C
Cuts at: 0 745 1578 2094
Size: 745 833 516
Fragments arranged by size:
833 745 516
BanII G_rGCy'C
Cuts at: 0 1093 1547 1732 2094
Size: 1093 454 185 362
Fragments arranged by size:
1093 454 362 185
BbsI GAAGACnn'nnnn_
Cuts at: 0 2078 2094
Size: 2078 16
BbvCI CC'TCA_GC
Cuts at: 0 1290 1501 1583 2094
Size: 1290 211 82 511
Fragments arranged by size:
1290 511 211 82
BciVI GTATCCnnnnn_n'
Cuts at: 0 517 2094
Size: 517 1577
BlpI GC'TnA_GC
Cuts at: 0 1712 2094
Size: 1712 382
BmrI ACTGGGnnnn_n'
Cuts at: 0 1401 1735 2094
Size: 1401 334 359
Fragments arranged by size:
1401 359 334
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1566 2094
Size: 1566 528
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 322 642 983 1663 2094
Size: 280 42 320 341 680 431
Fragments arranged by size:
680 431 341 320 280 42
Bpu10I CC'TnA_GC
Cuts at: 0 700 1290 1501 1583 2094
Size: 700 590 211 82 511
Fragments arranged by size:
700 590 511 211 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 626 2094
Size: 238 388 1468
Fragments arranged by size:
1468 388 238
BsaI GGTCTCn'nnnn_
Cuts at: 0 908 1096 2094
Size: 908 188 998
Fragments arranged by size:
998 908 188
BsaAI yAC'GTr
Cuts at: 0 907 2094
Size: 907 1187
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1181 1211 1661 1691 2094
Size: 1181 30 450 30 403
Fragments arranged by size:
1181 450 403 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1537 2094
Size: 1537 557
BseYI C'CCAG_C
Cuts at: 0 918 1237 2094
Size: 918 319 857
Fragments arranged by size:
918 857 319
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1136 1744 1885 2094
Size: 1136 608 141 209
Fragments arranged by size:
1136 608 209 141
BsiHKAI G_wGCw'C
Cuts at: 0 904 1056 1093 1547 2094
Size: 904 152 37 454 547
Fragments arranged by size:
904 547 454 152 37
BsmI GAATG_Cn'
Cuts at: 0 865 1160 2094
Size: 865 295 934
Fragments arranged by size:
934 865 295
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1203 2094
Size: 1203 891
Bsp1286I G_dGCh'C
Cuts at: 0 904 1056 1093 1547 1732 2094
Size: 904 152 37 454 185 362
Fragments arranged by size:
904 454 362 185 152 37
BspHI T'CATG_A
Cuts at: 0 1931 2094
Size: 1931 163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 671 2094
Size: 671 1423
BsrDI GCAATG_nn'
Cuts at: 0 280 2094
Size: 280 1814
BsrGI T'GTAC_A
Cuts at: 0 987 1625 1631 2094
Size: 987 638 6 463
Fragments arranged by size:
987 638 463 6
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1231 2094
Size: 1231 863
BstBI TT'CG_AA
Cuts at: 0 56 2094
Size: 56 2038
BstEII G'GTnAC_C
Cuts at: 0 1381 2094
Size: 1381 713
BstXI CCAn_nnnn'nTGG
Cuts at: 0 961 2094
Size: 961 1133
Bsu36I CC'TnA_GG
Cuts at: 0 799 1112 1297 2094
Size: 799 313 185 797
Fragments arranged by size:
799 797 313 185
BtgI C'CryG_G
Cuts at: 0 2054 2094
Size: 2054 40
BtsI GCAGTG_nn'
Cuts at: 0 1008 1938 2094
Size: 1008 930 156
Fragments arranged by size:
1008 930 156
DraI TTT'AAA
Cuts at: 0 303 2094
Size: 303 1791
DraIII CAC_nnn'GTG
Cuts at: 0 244 1176 1944 2094
Size: 244 932 768 150
Fragments arranged by size:
932 768 244 150
EarI CTCTTCn'nnn_
Cuts at: 0 587 1371 1933 2021 2094
Size: 587 784 562 88 73
Fragments arranged by size:
784 587 562 88 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1576 1878 2094
Size: 1576 302 216
Fragments arranged by size:
1576 302 216
EcoICRI GAG'CTC
Cuts at: 0 1091 1545 2094
Size: 1091 454 549
Fragments arranged by size:
1091 549 454
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 280 322 642 691 983 1150 1663
Size: 55 225 42 320 49 292 167 513
Cuts at: 1663 2094
Size: 431
Fragments arranged by size:
513 431 320 292 225 167 55 49
42
EcoO109I rG'GnC_Cy
Cuts at: 0 938 1017 1837 1950 2094
Size: 938 79 820 113 144
Fragments arranged by size:
938 820 144 113 79
EcoRI G'AATT_C
Cuts at: 0 259 2094
Size: 259 1835
HaeII r_GCGC'y
Cuts at: 0 1789 2094
Size: 1789 305
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1181 1213 1273 1305 2094
Size: 1181 32 60 32 789
Fragments arranged by size:
1181 789 60 32 32
HincII GTy'rAC
Cuts at: 0 615 2094
Size: 615 1479
HindIII A'AGCT_T
Cuts at: 0 606 1714 2094
Size: 606 1108 380
Fragments arranged by size:
1108 606 380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1778 2094
Size: 1778 316
MslI CAynn'nnrTG
Cuts at: 0 1795 2094
Size: 1795 299
MspA1I CmG'CkG
Cuts at: 0 620 1036 1187 1575 1683 2056 2094
Size: 620 416 151 388 108 373 38
Fragments arranged by size:
620 416 388 373 151 108 38
NspI r_CATG'y
Cuts at: 0 434 492 1632 1992 2094
Size: 434 58 1140 360 102
Fragments arranged by size:
1140 434 360 102 58
PasI CC'CwG_GG
Cuts at: 0 688 2094
Size: 688 1406
PciI A'CATG_T
Cuts at: 0 488 1628 2094
Size: 488 1140 466
Fragments arranged by size:
1140 488 466
PflMI CCAn_nnn'nTGG
Cuts at: 0 445 769 1747 2094
Size: 445 324 978 347
Fragments arranged by size:
978 445 347 324
PfoI T'CCnGG_A
Cuts at: 0 960 1130 2094
Size: 960 170 964
Fragments arranged by size:
964 960 170
PmlI CAC'GTG
Cuts at: 0 907 2094
Size: 907 1187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 20 52 711 743 2094
Size: 20 32 659 32 1351
Fragments arranged by size:
1351 659 32 32 20
PpuMI rG'GwC_Cy
Cuts at: 0 1837 1950 2094
Size: 1837 113 144
Fragments arranged by size:
1837 144 113
PsiI TTA'TAA
Cuts at: 0 418 2094
Size: 418 1676
PstI C_TGCA'G
Cuts at: 0 758 1025 1374 2094
Size: 758 267 349 720
Fragments arranged by size:
758 720 349 267
PvuII CAG'CTG
Cuts at: 0 620 1036 1575 1683 2094
Size: 620 416 539 108 411
Fragments arranged by size:
620 539 416 411 108
SacI G_AGCT'C
Cuts at: 0 1093 1547 2094
Size: 1093 454 547
Fragments arranged by size:
1093 547 454
SacII CC_GC'GG
Cuts at: 0 2057 2094
Size: 2057 37
SanDI GG'GwC_CC
Cuts at: 0 1837 2094
Size: 1837 257
SapI GCTCTTCn'nnn_
Cuts at: 0 1933 2094
Size: 1933 161
SbfI CC_TGCA'GG
Cuts at: 0 1025 2094
Size: 1025 1069
SexAI A'CCwGG_T
Cuts at: 0 1172 1967 2094
Size: 1172 795 127
Fragments arranged by size:
1172 795 127
SfcI C'TryA_G
Cuts at: 0 754 1021 1370 2094
Size: 754 267 349 724
Fragments arranged by size:
754 724 349 267
SmlI C'TyrA_G
Cuts at: 0 217 641 1414 2094
Size: 217 424 773 680
Fragments arranged by size:
773 680 424 217
SphI G_CATG'C
Cuts at: 0 1992 2094
Size: 1992 102
StuI AGG'CCT
Cuts at: 0 797 2094
Size: 797 1297
StyI C'CwwG_G
Cuts at: 0 394 779 1317 1661 1843 2094
Size: 394 385 538 344 182 251
Fragments arranged by size:
538 394 385 344 251 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1343 1864 2094
Size: 1343 521 230
Fragments arranged by size:
1343 521 230
TatI w'GTAC_w
Cuts at: 0 987 1625 1631 2094
Size: 987 638 6 463
Fragments arranged by size:
987 638 463 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1108 1140 1661 1693 2094
Size: 1108 32 521 32 401
Fragments arranged by size:
1108 521 401 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 516 2094
Size: 516 1578
XbaI T'CTAG_A
Cuts at: 0 186 2094
Size: 186 1908
XmnI GAAnn'nnTTC
Cuts at: 0 35 122 182 2094
Size: 35 87 60 1912
Fragments arranged by size:
1912 87 60 35
Enzymes that do cut:
AcuI AfeI AflII AflIII AlfI AloI AlwNI ApoI
AvaI AvrII BanI BanII BbsI BbvCI BciVI BlpI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrGI BstAPI BstBI BstEII BstXI Bsu36I
BtgI BtsI DraI DraIII EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI HaeII Hin4I HincII HindIII MmeI MslI
MspA1I NspI PasI PciI PflMI PfoI PmlI PpiI
PpuMI PsiI PstI PvuII SacI SacII SanDI SapI
SbfI SexAI SfcI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AgeI AhdI AleI
ApaI ApaLI AscI AseI AsiSI BaeI BamHI BbeI
BcgI BclI BfrBI BglI BglII Bme1580I BmgBI BmtI
BsaBI BsaHI BsaWI BsiEI BsiWI BspEI BsrBI BsrFI
BssHII BssSI BstYI BstZ17I BtgZI ClaI CspCI DrdI
EaeI EagI EcoNI EcoRV FalI FseI FspI FspAI
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PshAI PspOMI PspXI PsrI PvuI RsrII SalI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI XcmI XhoI XmaI ZraI