Restriction map for
pFN21AE5779
(Linear) (Six-base) MAPSORT of: KIEE5779.seq Check: 604 from: 1 to: 1935
KIEE5779.orf
With 182 enzymes: SgfI *
December 5, 2014 13:10 ..
AccI GT'mk_AC
Cuts at: 0 863 1935
Size: 863 1072
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1139 1897 1935
Size: 1139 758 38
Fragments arranged by size:
1139 758 38
AflIII A'CryG_T
Cuts at: 0 157 1935
Size: 157 1778
AleI CACnn'nnGTG
Cuts at: 0 1526 1935
Size: 1526 409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 478 1935
Size: 478 1457
AlwNI CAG_nnn'CTG
Cuts at: 0 304 1858 1935
Size: 304 1554 77
Fragments arranged by size:
1554 304 77
ApoI r'AATT_y
Cuts at: 0 119 127 1230 1625 1935
Size: 119 8 1103 395 310
Fragments arranged by size:
1103 395 310 119 8
AseI AT'TA_AT
Cuts at: 0 1276 1935
Size: 1276 659
BamHI G'GATC_C
Cuts at: 0 875 1935
Size: 875 1060
BanI G'GyrC_C
Cuts at: 0 664 1722 1935
Size: 664 1058 213
Fragments arranged by size:
1058 664 213
BanII G_rGCy'C
Cuts at: 0 1556 1932 1935
Size: 1556 376 3
Fragments arranged by size:
1556 376 3
BbvCI CC'TCA_GC
Cuts at: 0 965 1935
Size: 965 970
BciVI GTATCCnnnnn_n'
Cuts at: 0 1123 1935
Size: 1123 812
BlpI GC'TnA_GC
Cuts at: 0 437 1935
Size: 437 1498
Bme1580I G_kGCm'C
Cuts at: 0 1725 1935
Size: 1725 210
BmrI ACTGGGnnnn_n'
Cuts at: 0 881 1520 1935
Size: 881 639 415
Fragments arranged by size:
881 639 415
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1423 1935
Size: 1423 512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 1220 1346 1687 1935
Size: 886 334 126 341 248
Fragments arranged by size:
886 341 334 248 126
Bpu10I CC'TnA_GC
Cuts at: 0 965 1935
Size: 965 970
BsaI GGTCTCn'nnnn_
Cuts at: 0 1285 1935
Size: 1285 650
BsaBI GATnn'nnATC
Cuts at: 0 418 1935
Size: 418 1517
BsaWI w'CCGG_w
Cuts at: 0 146 1935
Size: 146 1789
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1767 1797 1935
Size: 1767 30 138
Fragments arranged by size:
1767 138 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 129 1412 1935
Size: 129 1283 523
Fragments arranged by size:
1283 523 129
BsiHKAI G_wGCw'C
Cuts at: 0 1334 1409 1932 1935
Size: 1334 75 523 3
Fragments arranged by size:
1334 523 75 3
BsmI GAATG_Cn'
Cuts at: 0 453 1935
Size: 453 1482
BsmBI CGTCTCn'nnnn_
Cuts at: 0 182 1935
Size: 182 1753
Bsp1286I G_dGCh'C
Cuts at: 0 1334 1409 1556 1725 1932 1935
Size: 1334 75 147 169 207 3
Fragments arranged by size:
1334 207 169 147 75 3
BspEI T'CCGG_A
Cuts at: 0 146 1935
Size: 146 1789
BspHI T'CATG_A
Cuts at: 0 581 1838 1935
Size: 581 1257 97
Fragments arranged by size:
1257 581 97
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 566 1935
Size: 566 1369
BsrDI GCAATG_nn'
Cuts at: 0 1605 1935
Size: 1605 330
BsrGI T'GTAC_A
Cuts at: 0 29 1544 1935
Size: 29 1515 391
Fragments arranged by size:
1515 391 29
BstBI TT'CG_AA
Cuts at: 0 1623 1935
Size: 1623 312
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1488 1935
Size: 1488 447
BstYI r'GATC_y
Cuts at: 0 875 927 1780 1935
Size: 875 52 853 155
Fragments arranged by size:
875 853 155 52
Bsu36I CC'TnA_GG
Cuts at: 0 1746 1868 1935
Size: 1746 122 67
Fragments arranged by size:
1746 122 67
BtgI C'CryG_G
Cuts at: 0 1089 1935
Size: 1089 846
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 141 176 1382 1417 1935
Size: 141 35 1206 35 518
Fragments arranged by size:
1206 518 141 35 35
DraI TTT'AAA
Cuts at: 0 1592 1935
Size: 1592 343
DraIII CAC_nnn'GTG
Cuts at: 0 203 1935
Size: 203 1732
EaeI y'GGCC_r
Cuts at: 0 974 1935
Size: 974 961
EarI CTCTTCn'nnn_
Cuts at: 0 509 1462 1935
Size: 509 953 473
Fragments arranged by size:
953 509 473
EcoICRI GAG'CTC
Cuts at: 0 1930 1935
Size: 1930 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 1139 1220 1346 1687 1897 1935
Size: 886 253 81 126 341 210 38
Fragments arranged by size:
886 341 253 210 126 81 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1465 1733 1935
Size: 1465 268 202
Fragments arranged by size:
1465 268 202
EcoO109I rG'GnC_Cy
Cuts at: 0 569 653 1935
Size: 569 84 1282
Fragments arranged by size:
1282 569 84
EcoRI G'AATT_C
Cuts at: 0 119 127 1230 1625 1935
Size: 119 8 1103 395 310
Fragments arranged by size:
1103 395 310 119 8
EcoRV GAT'ATC
Cuts at: 0 1029 1935
Size: 1029 906
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 483 1093 1935
Size: 483 610 842
Fragments arranged by size:
842 610 483
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1138 1170 1477 1509 1820 1852 1935
Size: 1138 32 307 32 311 32 83
Fragments arranged by size:
1138 311 307 83 32 32 32
MscI TGG'CCA
Cuts at: 0 976 1935
Size: 976 959
MslI CAynn'nnrTG
Cuts at: 0 586 850 1526 1935
Size: 586 264 676 409
Fragments arranged by size:
676 586 409 264
MspA1I CmG'CkG
Cuts at: 0 27 436 1174 1935
Size: 27 409 738 761
Fragments arranged by size:
761 738 409 27
NcoI C'CATG_G
Cuts at: 0 1089 1935
Size: 1089 846
NdeI CA'TA_TG
Cuts at: 0 1108 1935
Size: 1108 827
NspI r_CATG'y
Cuts at: 0 161 1346 1935
Size: 161 1185 589
Fragments arranged by size:
1185 589 161
PasI CC'CwG_GG
Cuts at: 0 658 1935
Size: 658 1277
PciI A'CATG_T
Cuts at: 0 157 1935
Size: 157 1778
PpuMI rG'GwC_Cy
Cuts at: 0 569 653 1935
Size: 569 84 1282
Fragments arranged by size:
1282 569 84
PshAI GACnn'nnGTC
Cuts at: 0 567 1935
Size: 567 1368
PstI C_TGCA'G
Cuts at: 0 381 1646 1935
Size: 381 1265 289
Fragments arranged by size:
1265 381 289
PvuII CAG'CTG
Cuts at: 0 27 436 1174 1935
Size: 27 409 738 761
Fragments arranged by size:
761 738 409 27
SacI G_AGCT'C
Cuts at: 0 1932 1935
Size: 1932 3
SapI GCTCTTCn'nnn_
Cuts at: 0 509 1935
Size: 509 1426
SbfI CC_TGCA'GG
Cuts at: 0 381 1935
Size: 381 1554
SfcI C'TryA_G
Cuts at: 0 87 377 1642 1935
Size: 87 290 1265 293
Fragments arranged by size:
1265 293 290 87
SphI G_CATG'C
Cuts at: 0 1346 1935
Size: 1346 589
StyI C'CwwG_G
Cuts at: 0 1089 1466 1935
Size: 1089 377 469
Fragments arranged by size:
1089 469 377
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 79 1428 1520 1935
Size: 79 1349 92 415
Fragments arranged by size:
1349 415 92 79
TatI w'GTAC_w
Cuts at: 0 2 29 1544 1935
Size: 2 27 1515 391
Fragments arranged by size:
1515 391 27 2
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1767 1799 1935
Size: 1767 32 136
Fragments arranged by size:
1767 136 32
XbaI T'CTAG_A
Cuts at: 0 1386 1935
Size: 1386 549
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApoI AseI
BamHI BanI BanII BbvCI BciVI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaBI BsaWI BsaXI BseRI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI
BsrGI BstBI BstXI BstYI Bsu36I BtgI CspCI DraI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI Hin4I MscI MslI MspA1I NcoI NdeI
NspI PasI PciI PpuMI PshAI PstI PvuII SacI
SapI SbfI SfcI SphI StyI TaqII TatI TstI
XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BbeI BbsI BcgI BclI BfrBI BglI BglII BmgBI
BmtI BpuEI BsaAI BsaHI BseYI BsgI BsiEI BsiWI
BsrBI BsrFI BssHII BssSI BstAPI BstEII BstZ17I BtgZI
BtsI ClaI DrdI EagI EciI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PflMI PfoI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI StuI SwaI Tth111I XcmI XhoI
XmaI XmnI ZraI