Restriction map for
pFN21AE5777
(Linear) (Six-base) MAPSORT of: KIEE5777.seq Check: 4689 from: 1 to: 1896
KIEE5777.orf
With 182 enzymes: SgfI *
December 5, 2014 13:10 ..
AatII G_ACGT'C
Cuts at: 0 1255 1896
Size: 1255 641
AccI GT'mk_AC
Cuts at: 0 815 1040 1820 1883 1896
Size: 815 225 780 63 13
Fragments arranged by size:
815 780 225 63 13
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 790 1793 1896
Size: 790 1003 103
Fragments arranged by size:
1003 790 103
AhdI GACnn_n'nnGTC
Cuts at: 0 1827 1896
Size: 1827 69
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 859 891 1896
Size: 859 32 1005
Fragments arranged by size:
1005 859 32
AlwNI CAG_nnn'CTG
Cuts at: 0 53 1670 1732 1896
Size: 53 1617 62 164
Fragments arranged by size:
1617 164 62 53
ApoI r'AATT_y
Cuts at: 0 91 228 565 1896
Size: 91 137 337 1331
Fragments arranged by size:
1331 337 137 91
AvrII C'CTAG_G
Cuts at: 0 1084 1896
Size: 1084 812
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 757 790 1896
Size: 757 33 1106
Fragments arranged by size:
1106 757 33
BanI G'GyrC_C
Cuts at: 0 238 1076 1896
Size: 238 838 820
Fragments arranged by size:
838 820 238
BanII G_rGCy'C
Cuts at: 0 1516 1694 1896
Size: 1516 178 202
Fragments arranged by size:
1516 202 178
BbeI G_GCGC'C
Cuts at: 0 1080 1896
Size: 1080 816
BbsI GAAGACnn'nnnn_
Cuts at: 0 632 1184 1896
Size: 632 552 712
Fragments arranged by size:
712 632 552
BbvCI CC'TCA_GC
Cuts at: 0 1294 1896
Size: 1294 602
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1437 1471 1612 1646 1896
Size: 1437 34 141 34 250
Fragments arranged by size:
1437 250 141 34 34
BfrBI ATG'CAT
Cuts at: 0 402 1896
Size: 402 1494
BglI GCCn_nnn'nGGC
Cuts at: 0 1222 1276 1330 1896
Size: 1222 54 54 566
Fragments arranged by size:
1222 566 54 54
BglII A'GATC_T
Cuts at: 0 287 1896
Size: 287 1609
BlpI GC'TnA_GC
Cuts at: 0 354 1896
Size: 354 1542
Bme1580I G_kGCm'C
Cuts at: 0 122 243 1896
Size: 122 121 1653
Fragments arranged by size:
1653 122 121
BmrI ACTGGGnnnn_n'
Cuts at: 0 71 1535 1887 1896
Size: 71 1464 352 9
Fragments arranged by size:
1464 352 71 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 126 1896
Size: 126 1770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1051 1358 1896
Size: 1051 307 538
Fragments arranged by size:
1051 538 307
Bpu10I CC'TnA_GC
Cuts at: 0 1294 1348 1896
Size: 1294 54 548
Fragments arranged by size:
1294 548 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1530 1896
Size: 1530 366
BsaI GGTCTCn'nnnn_
Cuts at: 0 216 361 1092 1896
Size: 216 145 731 804
Fragments arranged by size:
804 731 216 145
BsaHI Gr'CG_yC
Cuts at: 0 1077 1252 1896
Size: 1077 175 644
Fragments arranged by size:
1077 644 175
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 99 691 1896
Size: 99 592 1205
Fragments arranged by size:
1205 592 99
BseYI C'CCAG_C
Cuts at: 0 1206 1271 1592 1896
Size: 1206 65 321 304
Fragments arranged by size:
1206 321 304 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1174 1896
Size: 1174 722
BsiHKAI G_wGCw'C
Cuts at: 0 1516 1694 1765 1896
Size: 1516 178 71 131
Fragments arranged by size:
1516 178 131 71
Bsp1286I G_dGCh'C
Cuts at: 0 122 243 1516 1694 1765 1896
Size: 122 121 1273 178 71 131
Fragments arranged by size:
1273 178 131 122 121 71
BsrDI GCAATG_nn'
Cuts at: 0 1112 1896
Size: 1112 784
BssSI C'ACGA_G
Cuts at: 0 273 997 1896
Size: 273 724 899
Fragments arranged by size:
899 724 273
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1471 1534 1896
Size: 1471 63 362
Fragments arranged by size:
1471 362 63
BstYI r'GATC_y
Cuts at: 0 287 555 1896
Size: 287 268 1341
Fragments arranged by size:
1341 287 268
BstZ17I GTA'TAC
Cuts at: 0 1884 1896
Size: 1884 12
BtgI C'CryG_G
Cuts at: 0 1464 1470 1527 1533 1563 1701 1896
Size: 1464 6 57 6 30 138 195
Fragments arranged by size:
1464 195 138 57 30 6 6
BtsI GCAGTG_nn'
Cuts at: 0 35 1896
Size: 35 1861
EaeI y'GGCC_r
Cuts at: 0 1277 1331 1467 1530 1704 1896
Size: 1277 54 136 63 174 192
Fragments arranged by size:
1277 192 174 136 63 54
EarI CTCTTCn'nnn_
Cuts at: 0 145 1101 1846 1896
Size: 145 956 745 50
Fragments arranged by size:
956 745 145 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 205 1896
Size: 205 1691
EcoICRI GAG'CTC
Cuts at: 0 1514 1692 1896
Size: 1514 178 204
Fragments arranged by size:
1514 204 178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 790 1051 1358 1793 1896
Size: 790 261 307 435 103
Fragments arranged by size:
790 435 307 261 103
EcoO109I rG'GnC_Cy
Cuts at: 0 50 417 1069 1215 1323 1596 1674 1896
Size: 50 367 652 146 108 273 78 222
Fragments arranged by size:
652 367 273 222 146 108 78 50
EcoRV GAT'ATC
Cuts at: 0 854 987 1896
Size: 854 133 909
Fragments arranged by size:
909 854 133
HaeII r_GCGC'y
Cuts at: 0 1080 1527 1896
Size: 1080 447 369
Fragments arranged by size:
1080 447 369
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 197 229 1345 1377 1708 1740 1813 1845
Size: 197 32 1116 32 331 32 73 32
Cuts at: 1845 1896
Size: 51
Fragments arranged by size:
1116 331 197 73 51 32 32 32
32
HincII GTy'rAC
Cuts at: 0 795 1821 1896
Size: 795 1026 75
Fragments arranged by size:
1026 795 75
HpaI GTT'AAC
Cuts at: 0 795 1896
Size: 795 1101
KasI G'GCGC_C
Cuts at: 0 1076 1896
Size: 1076 820
MfeI C'AATT_G
Cuts at: 0 896 1896
Size: 896 1000
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 1896
Size: 549 1347
MscI TGG'CCA
Cuts at: 0 1279 1333 1469 1532 1706 1896
Size: 1279 54 136 63 174 190
Fragments arranged by size:
1279 190 174 136 63 54
MslI CAynn'nnrTG
Cuts at: 0 500 667 1388 1469 1487 1532 1896
Size: 500 167 721 81 18 45 364
Fragments arranged by size:
721 500 364 167 81 45 18
MspA1I CmG'CkG
Cuts at: 0 1275 1896
Size: 1275 621
NarI GG'CG_CC
Cuts at: 0 1077 1896
Size: 1077 819
NcoI C'CATG_G
Cuts at: 0 1464 1470 1527 1533 1563 1701 1896
Size: 1464 6 57 6 30 138 195
Fragments arranged by size:
1464 195 138 57 30 6 6
NdeI CA'TA_TG
Cuts at: 0 1889 1896
Size: 1889 7
NsiI A_TGCA'T
Cuts at: 0 404 1896
Size: 404 1492
NspI r_CATG'y
Cuts at: 0 18 1896
Size: 18 1878
PasI CC'CwG_GG
Cuts at: 0 1756 1896
Size: 1756 140
PflMI CCAn_nnn'nTGG
Cuts at: 0 1137 1756 1896
Size: 1137 619 140
Fragments arranged by size:
1137 619 140
PpuMI rG'GwC_Cy
Cuts at: 0 50 417 1069 1596 1896
Size: 50 367 652 527 300
Fragments arranged by size:
652 527 367 300 50
PstI C_TGCA'G
Cuts at: 0 885 1896
Size: 885 1011
PvuII CAG'CTG
Cuts at: 0 1275 1896
Size: 1275 621
SacI G_AGCT'C
Cuts at: 0 1516 1694 1896
Size: 1516 178 202
Fragments arranged by size:
1516 202 178
SalI G'TCGA_C
Cuts at: 0 1819 1896
Size: 1819 77
SanDI GG'GwC_CC
Cuts at: 0 417 1896
Size: 417 1479
SexAI A'CCwGG_T
Cuts at: 0 593 1896
Size: 593 1303
SfcI C'TryA_G
Cuts at: 0 536 881 1059 1896
Size: 536 345 178 837
Fragments arranged by size:
837 536 345 178
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1276 1330 1896
Size: 1276 54 566
Fragments arranged by size:
1276 566 54
SfoI GGC'GCC
Cuts at: 0 1078 1896
Size: 1078 818
SmlI C'TyrA_G
Cuts at: 0 1509 1896
Size: 1509 387
SphI G_CATG'C
Cuts at: 0 18 1896
Size: 18 1878
StuI AGG'CCT
Cuts at: 0 586 1896
Size: 586 1310
StyI C'CwwG_G
Cuts at: 0 1084 1464 1470 1527 1533 1563 1701 1896
Size: 1084 380 6 57 6 30 138 195
Fragments arranged by size:
1084 380 195 138 57 30 6 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 240 512 1748 1896
Size: 240 272 1236 148
Fragments arranged by size:
1236 272 240 148
Tth111I GACn'n_nGTC
Cuts at: 0 1183 1807 1896
Size: 1183 624 89
Fragments arranged by size:
1183 624 89
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1540 1896
Size: 1540 356
XmnI GAAnn'nnTTC
Cuts at: 0 139 1896
Size: 139 1757
ZraI GAC'GTC
Cuts at: 0 1253 1896
Size: 1253 643
Enzymes that do cut:
AatII AccI AcuI AhdI AloI AlwNI ApoI AvrII
BaeI BanI BanII BbeI BbsI BbvCI BcgI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I
BsrDI BssSI BstXI BstYI BstZ17I BtgI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRV HaeII Hin4I
HincII HpaI KasI MfeI MmeI MscI MslI MspA1I
NarI NcoI NdeI NsiI NspI PasI PflMI PpuMI
PstI PvuII SacI SalI SanDI SexAI SfcI SfiI
SfoI SmlI SphI StuI StyI TaqII Tth111I XcmI
XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI ApaI ApaLI AscI AseI AsiSI AvaI BamHI
BciVI BclI BmgBI BmtI BsaAI BsaBI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BstAPI BstBI BstEII Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRI
FalI FseI FspI FspAI HindIII KpnI MluI NaeI
NgoMIV NheI NotI NruI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SapI SbfI ScaI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI TatI TstI XbaI
XhoI XmaI