Restriction map for
pFN21AE5775
(Linear) (Six-base) MAPSORT of: KIEE5775.seq Check: 21 from: 1 to: 1872
KIEE5775.orf
With 182 enzymes: SgfI *
December 5, 2014 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 106 1872
Size: 106 1766
Acc65I G'GTAC_C
Cuts at: 0 817 1872
Size: 817 1055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 606 805 1228 1264 1306 1872
Size: 606 199 423 36 42 566
Fragments arranged by size:
606 566 423 199 42 36
AflIII A'CryG_T
Cuts at: 0 479 1872
Size: 479 1393
AhdI GACnn_n'nnGTC
Cuts at: 0 852 1872
Size: 852 1020
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 458 1872
Size: 458 1414
AlwNI CAG_nnn'CTG
Cuts at: 0 159 1757 1872
Size: 159 1598 115
Fragments arranged by size:
1598 159 115
ApaLI G'TGCA_C
Cuts at: 0 519 1872
Size: 519 1353
ApoI r'AATT_y
Cuts at: 0 36 238 248 316 894 1312 1548 1872
Size: 36 202 10 68 578 418 236 324
Fragments arranged by size:
578 418 324 236 202 68 36 10
AvaI C'yCGr_G
Cuts at: 0 125 1676 1872
Size: 125 1551 196
Fragments arranged by size:
1551 196 125
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 809 842 1872
Size: 809 33 1030
Fragments arranged by size:
1030 809 33
BamHI G'GATC_C
Cuts at: 0 129 1872
Size: 129 1743
BanI G'GyrC_C
Cuts at: 0 11 817 1872
Size: 11 806 1055
Fragments arranged by size:
1055 806 11
BanII G_rGCy'C
Cuts at: 0 467 1291 1872
Size: 467 824 581
Fragments arranged by size:
824 581 467
BbeI G_GCGC'C
Cuts at: 0 15 1872
Size: 15 1857
BbsI GAAGACnn'nnnn_
Cuts at: 0 941 1213 1363 1872
Size: 941 272 150 509
Fragments arranged by size:
941 509 272 150
BciVI GTATCCnnnnn_n'
Cuts at: 0 494 1604 1872
Size: 494 1110 268
Fragments arranged by size:
1110 494 268
BclI T'GATC_A
Cuts at: 0 243 1872
Size: 243 1629
BglI GCCn_nnn'nGGC
Cuts at: 0 121 1872
Size: 121 1751
BglII A'GATC_T
Cuts at: 0 1031 1872
Size: 1031 841
BlpI GC'TnA_GC
Cuts at: 0 1297 1872
Size: 1297 575
Bme1580I G_kGCm'C
Cuts at: 0 523 1872
Size: 523 1349
BmgBI CAC'GTC
Cuts at: 0 480 1872
Size: 480 1392
BmrI ACTGGGnnnn_n'
Cuts at: 0 643 1821 1872
Size: 643 1178 51
Fragments arranged by size:
1178 643 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 1530 1872
Size: 88 1442 342
Fragments arranged by size:
1442 342 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1872
Size: 955 917
BsaAI yAC'GTr
Cuts at: 0 366 1872
Size: 366 1506
BsaHI Gr'CG_yC
Cuts at: 0 12 1872
Size: 12 1860
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1034 1064 1872
Size: 1034 30 808
Fragments arranged by size:
1034 808 30
BseYI C'CCAG_C
Cuts at: 0 203 1799 1872
Size: 203 1596 73
Fragments arranged by size:
1596 203 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 211 353 1090 1872
Size: 183 28 142 737 782
Fragments arranged by size:
782 737 183 142 28
BsiEI CG_ry'CG
Cuts at: 0 116 1872
Size: 116 1756
BsiHKAI G_wGCw'C
Cuts at: 0 82 523 1291 1872
Size: 82 441 768 581
Fragments arranged by size:
768 581 441 82
BsmI GAATG_Cn'
Cuts at: 0 1250 1872
Size: 1250 622
Bsp1286I G_dGCh'C
Cuts at: 0 82 467 523 1291 1872
Size: 82 385 56 768 581
Fragments arranged by size:
768 581 385 82 56
BspHI T'CATG_A
Cuts at: 0 1170 1872
Size: 1170 702
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 106 1872
Size: 106 1766
BsrBI CCG'CTC
Cuts at: 0 55 1872
Size: 55 1817
BsrDI GCAATG_nn'
Cuts at: 0 746 1276 1872
Size: 746 530 596
Fragments arranged by size:
746 596 530
BssSI C'ACGA_G
Cuts at: 0 229 1872
Size: 229 1643
BstAPI GCAn_nnn'nTGC
Cuts at: 0 159 1872
Size: 159 1713
BstEII G'GTnAC_C
Cuts at: 0 637 1872
Size: 637 1235
BstXI CCAn_nnnn'nTGG
Cuts at: 0 140 1872
Size: 140 1732
BstYI r'GATC_y
Cuts at: 0 129 1031 1389 1872
Size: 129 902 358 483
Fragments arranged by size:
902 483 358 129
BtgI C'CryG_G
Cuts at: 0 446 1872
Size: 446 1426
BtsI GCAGTG_nn'
Cuts at: 0 160 1228 1688 1872
Size: 160 1068 460 184
Fragments arranged by size:
1068 460 184 160
ClaI AT'CG_AT
Cuts at: 0 176 1872
Size: 176 1696
DraI TTT'AAA
Cuts at: 0 1578 1872
Size: 1578 294
DraIII CAC_nnn'GTG
Cuts at: 0 1671 1872
Size: 1671 201
EaeI y'GGCC_r
Cuts at: 0 113 1020 1872
Size: 113 907 852
Fragments arranged by size:
907 852 113
EagI C'GGCC_G
Cuts at: 0 113 1872
Size: 113 1759
EarI CTCTTCn'nnn_
Cuts at: 0 978 1872
Size: 978 894
EcoICRI GAG'CTC
Cuts at: 0 1289 1872
Size: 1289 583
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 606 805 1228 1264 1306 1530 1872
Size: 88 518 199 423 36 42 224 342
Fragments arranged by size:
518 423 342 224 199 88 42 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 599 1872
Size: 599 1273
EcoO109I rG'GnC_Cy
Cuts at: 0 1791 1872
Size: 1791 81
HaeII r_GCGC'y
Cuts at: 0 15 111 1872
Size: 15 96 1761
Fragments arranged by size:
1761 96 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 838 870 1034 1066 1078 1110 1872
Size: 838 32 164 32 12 32 762
Fragments arranged by size:
838 762 164 32 32 32 12
HindIII A'AGCT_T
Cuts at: 0 1001 1872
Size: 1001 871
KasI G'GCGC_C
Cuts at: 0 11 1872
Size: 11 1861
KpnI G_GTAC'C
Cuts at: 0 821 1872
Size: 821 1051
MscI TGG'CCA
Cuts at: 0 1022 1872
Size: 1022 850
MslI CAynn'nnrTG
Cuts at: 0 711 1856 1872
Size: 711 1145 16
Fragments arranged by size:
1145 711 16
MspA1I CmG'CkG
Cuts at: 0 207 494 859 1872
Size: 207 287 365 1013
Fragments arranged by size:
1013 365 287 207
NarI GG'CG_CC
Cuts at: 0 12 1872
Size: 12 1860
NcoI C'CATG_G
Cuts at: 0 446 1872
Size: 446 1426
NspI r_CATG'y
Cuts at: 0 1063 1872
Size: 1063 809
PasI CC'CwG_GG
Cuts at: 0 456 1872
Size: 456 1416
PfoI T'CCnGG_A
Cuts at: 0 698 978 1872
Size: 698 280 894
Fragments arranged by size:
894 698 280
PmlI CAC'GTG
Cuts at: 0 366 1872
Size: 366 1506
PstI C_TGCA'G
Cuts at: 0 155 1872
Size: 155 1717
PvuII CAG'CTG
Cuts at: 0 207 494 859 1872
Size: 207 287 365 1013
Fragments arranged by size:
1013 365 287 207
SacI G_AGCT'C
Cuts at: 0 1291 1872
Size: 1291 581
SfcI C'TryA_G
Cuts at: 0 151 1761 1872
Size: 151 1610 111
Fragments arranged by size:
1610 151 111
SfoI GGC'GCC
Cuts at: 0 13 1872
Size: 13 1859
SmlI C'TyrA_G
Cuts at: 0 970 1872
Size: 970 902
SpeI A'CTAG_T
Cuts at: 0 1423 1872
Size: 1423 449
StyI C'CwwG_G
Cuts at: 0 446 1188 1872
Size: 446 742 684
Fragments arranged by size:
742 684 446
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 217 1813 1872
Size: 217 1596 59
Fragments arranged by size:
1596 217 59
TatI w'GTAC_w
Cuts at: 0 1381 1872
Size: 1381 491
XbaI T'CTAG_A
Cuts at: 0 1254 1872
Size: 1254 618
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 453 1872
Size: 453 1419
XmnI GAAnn'nnTTC
Cuts at: 0 938 1872
Size: 938 934
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AlfI AlwNI ApaLI
ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BpmI BpuEI BsaAI BsaHI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI
BstAPI BstEII BstXI BstYI BtgI BtsI ClaI DraI
DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HindIII KasI KpnI MscI MslI MspA1I
NarI NcoI NspI PasI PfoI PmlI PstI PvuII
SacI SfcI SfoI SmlI SpeI StyI TaqII TatI
XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AleI AloI
ApaI AscI AseI AsiSI AvrII BbvCI BcgI BfrBI
BmtI BplI Bpu10I BsaI BsaBI BsaWI BseRI BsiWI
BsmBI BspEI BsrFI BsrGI BssHII BstBI BstZ17I Bsu36I
BtgZI CspCI DrdI EciI EcoRI EcoRV FalI FseI
FspI FspAI HincII HpaI MfeI MluI MmeI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SphI
SrfI SspI StuI SwaI TstI Tth111I XhoI XmaI
ZraI