Restriction map for
pFN21AE5767
(Linear) (Six-base) MAPSORT of: KIEE5767.seq Check: 3408 from: 1 to: 1797
KIEE5767.orf
With 182 enzymes: SgfI *
December 5, 2014 13:10 ..
AatII G_ACGT'C
Cuts at: 0 8 1797
Size: 8 1789
AccI GT'mk_AC
Cuts at: 0 950 1797
Size: 950 847
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 266 514 687 1797
Size: 112 154 248 173 1110
Fragments arranged by size:
1110 248 173 154 112
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 450 482 1797
Size: 450 32 1315
Fragments arranged by size:
1315 450 32
AlwNI CAG_nnn'CTG
Cuts at: 0 135 1797
Size: 135 1662
ApoI r'AATT_y
Cuts at: 0 43 443 767 829 1137 1797
Size: 43 400 324 62 308 660
Fragments arranged by size:
660 400 324 308 62 43
AseI AT'TA_AT
Cuts at: 0 1310 1797
Size: 1310 487
BanII G_rGCy'C
Cuts at: 0 711 1295 1797
Size: 711 584 502
Fragments arranged by size:
711 584 502
BbsI GAAGACnn'nnnn_
Cuts at: 0 277 356 1793 1797
Size: 277 79 1437 4
Fragments arranged by size:
1437 277 79 4
BciVI GTATCCnnnnn_n'
Cuts at: 0 785 1797
Size: 785 1012
BclI T'GATC_A
Cuts at: 0 1451 1797
Size: 1451 346
BglI GCCn_nnn'nGGC
Cuts at: 0 29 1797
Size: 29 1768
BmgBI CAC'GTC
Cuts at: 0 450 1797
Size: 450 1347
BmrI ACTGGGnnnn_n'
Cuts at: 0 583 995 1797
Size: 583 412 802
Fragments arranged by size:
802 583 412
BmtI G_CTAG'C
Cuts at: 0 1417 1797
Size: 1417 380
Bpu10I CC'TnA_GC
Cuts at: 0 1730 1797
Size: 1730 67
BsaI GGTCTCn'nnnn_
Cuts at: 0 989 1180 1797
Size: 989 191 617
Fragments arranged by size:
989 617 191
BsaHI Gr'CG_yC
Cuts at: 0 5 1797
Size: 5 1792
BsaWI w'CCGG_w
Cuts at: 0 385 1189 1797
Size: 385 804 608
Fragments arranged by size:
804 608 385
BseYI C'CCAG_C
Cuts at: 0 1600 1797
Size: 1600 197
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1008 1797
Size: 1008 789
BsiHKAI G_wGCw'C
Cuts at: 0 1295 1797
Size: 1295 502
BsmI GAATG_Cn'
Cuts at: 0 659 1797
Size: 659 1138
BsmBI CGTCTCn'nnnn_
Cuts at: 0 48 1797
Size: 48 1749
Bsp1286I G_dGCh'C
Cuts at: 0 711 1295 1797
Size: 711 584 502
Fragments arranged by size:
711 584 502
BspEI T'CCGG_A
Cuts at: 0 385 1797
Size: 385 1412
BspHI T'CATG_A
Cuts at: 0 1287 1797
Size: 1287 510
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 538 1797
Size: 538 1259
BsrFI r'CCGG_y
Cuts at: 0 23 1797
Size: 23 1774
BsrGI T'GTAC_A
Cuts at: 0 1402 1797
Size: 1402 395
BssSI C'ACGA_G
Cuts at: 0 51 728 1797
Size: 51 677 1069
Fragments arranged by size:
1069 677 51
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1066 1797
Size: 1066 731
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1017 1797
Size: 1017 780
BstYI r'GATC_y
Cuts at: 0 634 906 1180 1797
Size: 634 272 274 617
Fragments arranged by size:
634 617 274 272
BtsI GCAGTG_nn'
Cuts at: 0 821 1797
Size: 821 976
DraI TTT'AAA
Cuts at: 0 1434 1797
Size: 1434 363
EaeI y'GGCC_r
Cuts at: 0 1202 1797
Size: 1202 595
EarI CTCTTCn'nnn_
Cuts at: 0 218 238 1038 1506 1797
Size: 218 20 800 468 291
Fragments arranged by size:
800 468 291 218 20
EcoICRI GAG'CTC
Cuts at: 0 1293 1797
Size: 1293 504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 266 514 687 1797
Size: 112 154 248 173 1110
Fragments arranged by size:
1110 248 173 154 112
EcoRI G'AATT_C
Cuts at: 0 1137 1797
Size: 1137 660
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 438 1797
Size: 438 1359
MfeI C'AATT_G
Cuts at: 0 476 1797
Size: 476 1321
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 652 1158 1492 1797
Size: 374 278 506 334 305
Fragments arranged by size:
506 374 334 305 278
MslI CAynn'nnrTG
Cuts at: 0 1117 1797
Size: 1117 680
MspA1I CmG'CkG
Cuts at: 0 678 1002 1797
Size: 678 324 795
Fragments arranged by size:
795 678 324
NaeI GCC'GGC
Cuts at: 0 25 1797
Size: 25 1772
NdeI CA'TA_TG
Cuts at: 0 1071 1797
Size: 1071 726
NgoMIV G'CCGG_C
Cuts at: 0 23 1797
Size: 23 1774
NheI G'CTAG_C
Cuts at: 0 1413 1797
Size: 1413 384
PstI C_TGCA'G
Cuts at: 0 1146 1797
Size: 1146 651
PvuII CAG'CTG
Cuts at: 0 678 1002 1797
Size: 678 324 795
Fragments arranged by size:
795 678 324
SacI G_AGCT'C
Cuts at: 0 1295 1797
Size: 1295 502
SapI GCTCTTCn'nnn_
Cuts at: 0 1038 1506 1797
Size: 1038 468 291
Fragments arranged by size:
1038 468 291
SfcI C'TryA_G
Cuts at: 0 332 1142 1154 1797
Size: 332 810 12 643
Fragments arranged by size:
810 643 332 12
StyI C'CwwG_G
Cuts at: 0 453 1797
Size: 453 1344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1592 1797
Size: 1592 205
TatI w'GTAC_w
Cuts at: 0 1402 1699 1797
Size: 1402 297 98
Fragments arranged by size:
1402 297 98
Tth111I GACn'n_nGTC
Cuts at: 0 1108 1797
Size: 1108 689
XbaI T'CTAG_A
Cuts at: 0 540 1797
Size: 540 1257
XmnI GAAnn'nnTTC
Cuts at: 0 1283 1797
Size: 1283 514
ZraI GAC'GTC
Cuts at: 0 6 1797
Size: 6 1791
Enzymes that do cut:
AatII AccI AcuI AloI AlwNI ApoI AseI BanII
BbsI BciVI BclI BglI BmgBI BmrI BmtI Bpu10I
BsaI BsaHI BsaWI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrFI BsrGI BssSI BstAPI
BstXI BstYI BtsI DraI EaeI EarI EcoICRI Eco57MI
EcoRI FalI MfeI MmeI MslI MspA1I NaeI NdeI
NgoMIV NheI PstI PvuII SacI SapI SfcI StyI
TaqII TatI Tth111I XbaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BfrBI BglII
BlpI Bme1580I BplI BpmI BpuEI BsaAI BsaBI BsaXI
BseRI BsiEI BsiWI BsrBI BsrDI BssHII BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoNI EcoO109I EcoRV FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MscI NarI NcoI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI XcmI XhoI XmaI