Restriction map for
pFN21AE5762
(Linear) (Six-base) MAPSORT of: KIEE5762.seq Check: 9686 from: 1 to: 2529
KIEE5762.orf
With 182 enzymes: SgfI *
December 5, 2014 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2213 2529
Size: 2213 316
AatII G_ACGT'C
Cuts at: 0 1889 2529
Size: 1889 640
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 901 1576 2236 2529
Size: 298 603 675 660 293
Fragments arranged by size:
675 660 603 298 293
AfeI AGC'GCT
Cuts at: 0 1159 2529
Size: 1159 1370
AflIII A'CryG_T
Cuts at: 0 615 2529
Size: 615 1914
AleI CACnn'nnGTG
Cuts at: 0 298 658 688 1334 2529
Size: 298 360 30 646 1195
Fragments arranged by size:
1195 646 360 298 30
ApaI G_GGCC'C
Cuts at: 0 2061 2529
Size: 2061 468
ApaLI G'TGCA_C
Cuts at: 0 601 2529
Size: 601 1928
ApoI r'AATT_y
Cuts at: 0 265 487 2529
Size: 265 222 2042
Fragments arranged by size:
2042 265 222
AvaI C'yCGr_G
Cuts at: 0 367 1491 2209 2424 2522 2529
Size: 367 1124 718 215 98 7
Fragments arranged by size:
1124 718 367 215 98 7
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1496 1529 2529
Size: 1496 33 1000
Fragments arranged by size:
1496 1000 33
BamHI G'GATC_C
Cuts at: 0 203 1055 1373 1709 2529
Size: 203 852 318 336 820
Fragments arranged by size:
852 820 336 318 203
BanI G'GyrC_C
Cuts at: 0 1196 1229 2011 2071 2083 2529
Size: 1196 33 782 60 12 446
Fragments arranged by size:
1196 782 446 60 33 12
BanII G_rGCy'C
Cuts at: 0 1049 2061 2489 2529
Size: 1049 1012 428 40
Fragments arranged by size:
1049 1012 428 40
BbeI G_GCGC'C
Cuts at: 0 2087 2529
Size: 2087 442
BbsI GAAGACnn'nnnn_
Cuts at: 0 1117 2529
Size: 1117 1412
BciVI GTATCCnnnnn_n'
Cuts at: 0 851 2529
Size: 851 1678
BclI T'GATC_A
Cuts at: 0 1856 2529
Size: 1856 673
BglI GCCn_nnn'nGGC
Cuts at: 0 2107 2529
Size: 2107 422
BglII A'GATC_T
Cuts at: 0 1997 2471 2529
Size: 1997 474 58
Fragments arranged by size:
1997 474 58
Bme1580I G_kGCm'C
Cuts at: 0 605 695 1802 2061 2529
Size: 605 90 1107 259 468
Fragments arranged by size:
1107 605 468 259 90
BmgBI CAC'GTC
Cuts at: 0 72 1068 1602 1749 2529
Size: 72 996 534 147 780
Fragments arranged by size:
996 780 534 147 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 682 1092 1897 2529
Size: 682 410 805 632
Fragments arranged by size:
805 682 632 410
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 843 2529
Size: 843 1686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 813 898 1222 1444 1669 1957 2421 2529
Size: 813 85 324 222 225 288 464 108
Fragments arranged by size:
813 464 324 288 225 222 108 85
Bpu10I CC'TnA_GC
Cuts at: 0 1043 1546 2529
Size: 1043 503 983
Fragments arranged by size:
1043 983 503
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1615 2529
Size: 1615 914
BsaI GGTCTCn'nnnn_
Cuts at: 0 1772 2529
Size: 1772 757
BsaAI yAC'GTr
Cuts at: 0 1242 2529
Size: 1242 1287
BsaHI Gr'CG_yC
Cuts at: 0 1886 2084 2529
Size: 1886 198 445
Fragments arranged by size:
1886 445 198
BsaWI w'CCGG_w
Cuts at: 0 806 2168 2375 2444 2529
Size: 806 1362 207 69 85
Fragments arranged by size:
1362 806 207 85 69
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1642 1666 1672 1696 2529
Size: 1642 24 6 24 833
Fragments arranged by size:
1642 833 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 385 547 1312 1543 1671 2119 2529
Size: 385 162 765 231 128 448 410
Fragments arranged by size:
765 448 410 385 231 162 128
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1729 2529
Size: 1729 800
BsiEI CG_ry'CG
Cuts at: 0 1979 2529
Size: 1979 550
BsiHKAI G_wGCw'C
Cuts at: 0 605 626 1049 2529
Size: 605 21 423 1480
Fragments arranged by size:
1480 605 423 21
BsmI GAATG_Cn'
Cuts at: 0 324 2529
Size: 324 2205
BsmBI CGTCTCn'nnnn_
Cuts at: 0 976 2529
Size: 976 1553
Bsp1286I G_dGCh'C
Cuts at: 0 605 626 695 1049 1802 2061 2489 2529
Size: 605 21 69 354 753 259 428 40
Fragments arranged by size:
753 605 428 354 259 69 40 21
BspHI T'CATG_A
Cuts at: 0 1896 2529
Size: 1896 633
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 516 2213 2504 2529
Size: 516 1697 291 25
Fragments arranged by size:
1697 516 291 25
BsrDI GCAATG_nn'
Cuts at: 0 138 2529
Size: 138 2391
BsrFI r'CCGG_y
Cuts at: 0 98 1469 2529
Size: 98 1371 1060
Fragments arranged by size:
1371 1060 98
BsrGI T'GTAC_A
Cuts at: 0 1760 2529
Size: 1760 769
BssSI C'ACGA_G
Cuts at: 0 190 1714 2529
Size: 190 1524 815
Fragments arranged by size:
1524 815 190
BstAPI GCAn_nnn'nTGC
Cuts at: 0 177 2529
Size: 177 2352
BstEII G'GTnAC_C
Cuts at: 0 1837 1869 2529
Size: 1837 32 660
Fragments arranged by size:
1837 660 32
BstYI r'GATC_y
Cuts at: 0 203 1055 1373 1709 1997 2471 2501 2529
Size: 203 852 318 336 288 474 30 28
Fragments arranged by size:
852 474 336 318 288 203 30 28
Bsu36I CC'TnA_GG
Cuts at: 0 1145 2022 2529
Size: 1145 877 507
Fragments arranged by size:
1145 877 507
BtgI C'CryG_G
Cuts at: 0 1192 2075 2225 2529
Size: 1192 883 150 304
Fragments arranged by size:
1192 883 304 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 341 1277 2400 2529
Size: 341 936 1123 129
Fragments arranged by size:
1123 936 341 129
BtsI GCAGTG_nn'
Cuts at: 0 2003 2247 2353 2529
Size: 2003 244 106 176
Fragments arranged by size:
2003 244 176 106
DraIII CAC_nnn'GTG
Cuts at: 0 177 192 1137 2529
Size: 177 15 945 1392
Fragments arranged by size:
1392 945 177 15
EaeI y'GGCC_r
Cuts at: 0 74 137 668 902 1001 1244 1499 1604
Size: 74 63 531 234 99 243 255 105
Cuts at: 1604 1784 1847 1976 2099 2529
Size: 180 63 129 123 430
Fragments arranged by size:
531 430 255 243 234 180 129 123
105 99 74 63 63
EagI C'GGCC_G
Cuts at: 0 1976 2529
Size: 1976 553
EarI CTCTTCn'nnn_
Cuts at: 0 82 1699 2131 2529
Size: 82 1617 432 398
Fragments arranged by size:
1617 432 398 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 726 916 2529
Size: 726 190 1613
Fragments arranged by size:
1613 726 190
EcoICRI GAG'CTC
Cuts at: 0 1047 2529
Size: 1047 1482
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 813 898 901 1222 1444 1576 1669
Size: 298 515 85 3 321 222 132 93
Cuts at: 1669 1957 2236 2421 2529
Size: 288 279 185 108
Fragments arranged by size:
515 321 298 288 279 222 185 132
108 93 85 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1651 2529
Size: 1651 878
EcoO109I rG'GnC_Cy
Cuts at: 0 834 960 1346 2057 2058 2529
Size: 834 126 386 711 1 471
Fragments arranged by size:
834 711 471 386 126 1
EcoRI G'AATT_C
Cuts at: 0 265 2529
Size: 265 2264
FspI TGC'GCA
Cuts at: 0 1279 2529
Size: 1279 1250
HaeII r_GCGC'y
Cuts at: 0 1161 1834 2087 2529
Size: 1161 673 253 442
Fragments arranged by size:
1161 673 442 253
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 773 805 2529
Size: 773 32 1724
Fragments arranged by size:
1724 773 32
HincII GTy'rAC
Cuts at: 0 717 2529
Size: 717 1812
KasI G'GCGC_C
Cuts at: 0 2083 2529
Size: 2083 446
MluI A'CGCG_T
Cuts at: 0 615 2529
Size: 615 1914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2516 2529
Size: 2516 13
MscI TGG'CCA
Cuts at: 0 139 670 1003 1606 1849 2529
Size: 139 531 333 603 243 680
Fragments arranged by size:
680 603 531 333 243 139
MslI CAynn'nnrTG
Cuts at: 0 298 658 688 1334 2296 2529
Size: 298 360 30 646 962 233
Fragments arranged by size:
962 646 360 298 233 30
MspA1I CmG'CkG
Cuts at: 0 12 200 1011 1194 1227 1554 1572 1731
Size: 12 188 811 183 33 327 18 159
Cuts at: 1731 1981 2370 2529
Size: 250 389 159
Fragments arranged by size:
811 389 327 250 188 183 159 159
33 18 12
NarI GG'CG_CC
Cuts at: 0 2084 2529
Size: 2084 445
NcoI C'CATG_G
Cuts at: 0 2075 2529
Size: 2075 454
NspI r_CATG'y
Cuts at: 0 1702 2295 2346 2529
Size: 1702 593 51 183
Fragments arranged by size:
1702 593 183 51
PasI CC'CwG_GG
Cuts at: 0 566 2529
Size: 566 1963
PflMI CCAn_nnn'nTGG
Cuts at: 0 192 2390 2529
Size: 192 2198 139
Fragments arranged by size:
2198 192 139
PfoI T'CCnGG_A
Cuts at: 0 800 914 1646 1934 2529
Size: 800 114 732 288 595
Fragments arranged by size:
800 732 595 288 114
PmlI CAC'GTG
Cuts at: 0 1242 2529
Size: 1242 1287
PpuMI rG'GwC_Cy
Cuts at: 0 834 960 1346 2529
Size: 834 126 386 1183
Fragments arranged by size:
1183 834 386 126
PspOMI G'GGCC_C
Cuts at: 0 2057 2529
Size: 2057 472
PspXI vC'TCGA_Gb
Cuts at: 0 367 2529
Size: 367 2162
PstI C_TGCA'G
Cuts at: 0 290 767 2010 2501 2529
Size: 290 477 1243 491 28
Fragments arranged by size:
1243 491 477 290 28
PvuII CAG'CTG
Cuts at: 0 1011 1554 1731 2370 2529
Size: 1011 543 177 639 159
Fragments arranged by size:
1011 639 543 177 159
SacI G_AGCT'C
Cuts at: 0 1049 2529
Size: 1049 1480
SacII CC_GC'GG
Cuts at: 0 1195 2529
Size: 1195 1334
SanDI GG'GwC_CC
Cuts at: 0 960 1346 2529
Size: 960 386 1183
Fragments arranged by size:
1183 960 386
SapI GCTCTTCn'nnn_
Cuts at: 0 82 2529
Size: 82 2447
ScaI AGT'ACT
Cuts at: 0 892 2194 2529
Size: 892 1302 335
Fragments arranged by size:
1302 892 335
SfcI C'TryA_G
Cuts at: 0 286 763 2006 2497 2529
Size: 286 477 1243 491 32
Fragments arranged by size:
1243 491 477 286 32
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2107 2529
Size: 2107 422
SfoI GGC'GCC
Cuts at: 0 2085 2529
Size: 2085 444
SgrAI Cr'CCGG_yG
Cuts at: 0 1469 2529
Size: 1469 1060
SmaI CCC'GGG
Cuts at: 0 1493 2524 2529
Size: 1493 1031 5
Fragments arranged by size:
1493 1031 5
SmlI C'TyrA_G
Cuts at: 0 367 1630 2529
Size: 367 1263 899
Fragments arranged by size:
1263 899 367
StuI AGG'CCT
Cuts at: 0 407 1093 1150 2020 2161 2529
Size: 407 686 57 870 141 368
Fragments arranged by size:
870 686 407 368 141 57
StyI C'CwwG_G
Cuts at: 0 393 449 740 1151 1607 1787 1955 2075
Size: 393 56 291 411 456 180 168 120
Cuts at: 2075 2162 2258 2384 2529
Size: 87 96 126 145
Fragments arranged by size:
456 411 393 291 180 168 145 126
120 96 87 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1036 1389 1900 2529
Size: 1036 353 511 629
Fragments arranged by size:
1036 629 511 353
TatI w'GTAC_w
Cuts at: 0 890 1658 1718 1760 2192 2529
Size: 890 768 60 42 432 337
Fragments arranged by size:
890 768 432 337 60 42
Tth111I GACn'n_nGTC
Cuts at: 0 1888 2529
Size: 1888 641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 342 1357 2073 2094 2239 2529
Size: 342 1015 716 21 145 290
Fragments arranged by size:
1015 716 342 290 145 21
XhoI C'TCGA_G
Cuts at: 0 367 2529
Size: 367 2162
XmaI C'CCGG_G
Cuts at: 0 1491 2522 2529
Size: 1491 1031 7
Fragments arranged by size:
1491 1031 7
XmnI GAAnn'nnTTC
Cuts at: 0 91 2125 2529
Size: 91 2034 404
Fragments arranged by size:
2034 404 91
ZraI GAC'GTC
Cuts at: 0 1887 2529
Size: 1887 642
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AleI ApaI ApaLI
ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI
BciVI BclI BglI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII KasI MluI MmeI MscI MslI MspA1I
NarI NcoI NspI PasI PflMI PfoI PmlI PpuMI
PspOMI PspXI PstI PvuII SacI SacII SanDI SapI
ScaI SfcI SfiI SfoI SgrAI SmaI SmlI StuI
StyI TaqII TatI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AlfI AloI
AlwNI AscI AseI AsiSI AvrII BbvCI BcgI BfrBI
BlpI BmtI BsaBI BseYI BsiWI BspEI BsrBI BssHII
BstBI BstXI BstZ17I ClaI CspCI DraI DrdI EcoRV
FalI FseI FspAI HindIII HpaI KpnI MfeI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PpiI PshAI PsiI PsrI PvuI RsrII SalI
SbfI SexAI SgfI SnaBI SpeI SphI SrfI SspI
SwaI TstI XbaI