Restriction map for
pFN21AE5761
(Linear) (Six-base) MAPSORT of: KIEE5761.seq Check: 3377 from: 1 to: 2433
KIEE5761.orf
With 182 enzymes: SgfI *
December 5, 2014 13:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 203 268 856 955 2433
Size: 73 130 65 588 99 1478
Fragments arranged by size:
1478 588 130 99 73 65
AfeI AGC'GCT
Cuts at: 0 2188 2433
Size: 2188 245
AlwNI CAG_nnn'CTG
Cuts at: 0 508 1630 2212 2230 2433
Size: 508 1122 582 18 203
Fragments arranged by size:
1122 582 508 203 18
ApoI r'AATT_y
Cuts at: 0 867 1509 1743 2433
Size: 867 642 234 690
Fragments arranged by size:
867 690 642 234
AvaI C'yCGr_G
Cuts at: 0 540 645 1193 2433
Size: 540 105 548 1240
Fragments arranged by size:
1240 548 540 105
BanI G'GyrC_C
Cuts at: 0 456 2433
Size: 456 1977
BanII G_rGCy'C
Cuts at: 0 232 1309 2433
Size: 232 1077 1124
Fragments arranged by size:
1124 1077 232
BbsI GAAGACnn'nnnn_
Cuts at: 0 683 1763 2433
Size: 683 1080 670
Fragments arranged by size:
1080 683 670
BbvCI CC'TCA_GC
Cuts at: 0 1203 2433
Size: 1203 1230
BciVI GTATCCnnnnn_n'
Cuts at: 0 255 1656 2433
Size: 255 1401 777
Fragments arranged by size:
1401 777 255
BclI T'GATC_A
Cuts at: 0 372 2433
Size: 372 2061
BglI GCCn_nnn'nGGC
Cuts at: 0 2273 2433
Size: 2273 160
Bme1580I G_kGCm'C
Cuts at: 0 2264 2433
Size: 2264 169
BmrI ACTGGGnnnn_n'
Cuts at: 0 1156 1706 1850 1864 2433
Size: 1156 550 144 14 569
Fragments arranged by size:
1156 569 550 144 14
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 1357 2189 2320 2433
Size: 634 723 832 131 113
Fragments arranged by size:
832 723 634 131 113
Bpu10I CC'TnA_GC
Cuts at: 0 404 1203 2433
Size: 404 799 1230
Fragments arranged by size:
1230 799 404
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 927 2073 2433
Size: 927 1146 360
Fragments arranged by size:
1146 927 360
BsaI GGTCTCn'nnnn_
Cuts at: 0 895 1077 2433
Size: 895 182 1356
Fragments arranged by size:
1356 895 182
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1874 1904 2275 2305 2433
Size: 1874 30 371 30 128
Fragments arranged by size:
1874 371 128 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 332 832 1157 1633 2334 2433
Size: 332 500 325 476 701 99
Fragments arranged by size:
701 500 476 332 325 99
BseYI C'CCAG_C
Cuts at: 0 213 1319 2263 2294 2311 2433
Size: 213 1106 944 31 17 122
Fragments arranged by size:
1106 944 213 122 31 17
BsiEI CG_ry'CG
Cuts at: 0 1722 2433
Size: 1722 711
BsiHKAI G_wGCw'C
Cuts at: 0 232 979 1161 2433
Size: 232 747 182 1272
Fragments arranged by size:
1272 747 232 182
Bsp1286I G_dGCh'C
Cuts at: 0 232 979 1161 1309 2264 2433
Size: 232 747 182 148 955 169
Fragments arranged by size:
955 747 232 182 169 148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 598 820 1095 2118 2433
Size: 598 222 275 1023 315
Fragments arranged by size:
1023 598 315 275 222
BsrDI GCAATG_nn'
Cuts at: 0 318 2171 2433
Size: 318 1853 262
Fragments arranged by size:
1853 318 262
BsrFI r'CCGG_y
Cuts at: 0 269 2267 2433
Size: 269 1998 166
Fragments arranged by size:
1998 269 166
BsrGI T'GTAC_A
Cuts at: 0 1389 2433
Size: 1389 1044
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2379 2433
Size: 2379 54
BstYI r'GATC_y
Cuts at: 0 1275 2433
Size: 1275 1158
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 67 2290 2433
Size: 67 2223 143
Fragments arranged by size:
2223 143 67
DrdI GACnn_nn'nnGTC
Cuts at: 0 608 2433
Size: 608 1825
EaeI y'GGCC_r
Cuts at: 0 323 2433
Size: 323 2110
EarI CTCTTCn'nnn_
Cuts at: 0 323 584 749 893 1521 1734 2433
Size: 323 261 165 144 628 213 699
Fragments arranged by size:
699 628 323 261 213 165 144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 240 1882 2433
Size: 240 1642 551
Fragments arranged by size:
1642 551 240
EcoICRI GAG'CTC
Cuts at: 0 230 2433
Size: 230 2203
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 203 268 634 856 955 1357 2189
Size: 73 130 65 366 222 99 402 832
Cuts at: 2189 2320 2433
Size: 131 113
Fragments arranged by size:
832 402 366 222 131 130 113 99
73 65
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 451 2433
Size: 355 96 1982
Fragments arranged by size:
1982 355 96
EcoO109I rG'GnC_Cy
Cuts at: 0 183 2433
Size: 183 2250
EcoRI G'AATT_C
Cuts at: 0 867 1509 2433
Size: 867 642 924
Fragments arranged by size:
924 867 642
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 793 1660 1930 2433
Size: 793 867 270 503
Fragments arranged by size:
867 793 503 270
FspI TGC'GCA
Cuts at: 0 286 1294 2433
Size: 286 1008 1139
Fragments arranged by size:
1139 1008 286
HaeII r_GCGC'y
Cuts at: 0 2190 2433
Size: 2190 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1612 1644 1711 1743 2235 2267 2433
Size: 1612 32 67 32 492 32 166
Fragments arranged by size:
1612 492 166 67 32 32 32
HincII GTy'rAC
Cuts at: 0 578 852 1072 2433
Size: 578 274 220 1361
Fragments arranged by size:
1361 578 274 220
MfeI C'AATT_G
Cuts at: 0 1238 2433
Size: 1238 1195
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 2433
Size: 239 2194
MslI CAynn'nnrTG
Cuts at: 0 1546 2433
Size: 1546 887
MspA1I CmG'CkG
Cuts at: 0 1207 1335 2230 2298 2433
Size: 1207 128 895 68 135
Fragments arranged by size:
1207 895 135 128 68
NspI r_CATG'y
Cuts at: 0 202 1293 1551 2289 2433
Size: 202 1091 258 738 144
Fragments arranged by size:
1091 738 258 202 144
PasI CC'CwG_GG
Cuts at: 0 1960 1986 2433
Size: 1960 26 447
Fragments arranged by size:
1960 447 26
PflMI CCAn_nnn'nTGG
Cuts at: 0 1630 2212 2433
Size: 1630 582 221
Fragments arranged by size:
1630 582 221
PfoI T'CCnGG_A
Cuts at: 0 633 2142 2433
Size: 633 1509 291
Fragments arranged by size:
1509 633 291
PpuMI rG'GwC_Cy
Cuts at: 0 183 2433
Size: 183 2250
PshAI GACnn'nnGTC
Cuts at: 0 2054 2433
Size: 2054 379
PspXI vC'TCGA_Gb
Cuts at: 0 1193 2433
Size: 1193 1240
PstI C_TGCA'G
Cuts at: 0 1058 2433
Size: 1058 1375
PvuI CG_AT'CG
Cuts at: 0 1722 2433
Size: 1722 711
PvuII CAG'CTG
Cuts at: 0 1207 1335 2230 2298 2433
Size: 1207 128 895 68 135
Fragments arranged by size:
1207 895 135 128 68
SacI G_AGCT'C
Cuts at: 0 232 2433
Size: 232 2201
SanDI GG'GwC_CC
Cuts at: 0 183 2433
Size: 183 2250
SapI GCTCTTCn'nnn_
Cuts at: 0 584 2433
Size: 584 1849
SbfI CC_TGCA'GG
Cuts at: 0 1058 2433
Size: 1058 1375
SfcI C'TryA_G
Cuts at: 0 715 1054 2182 2368 2433
Size: 715 339 1128 186 65
Fragments arranged by size:
1128 715 339 186 65
SmlI C'TyrA_G
Cuts at: 0 906 1193 2052 2433
Size: 906 287 859 381
Fragments arranged by size:
906 859 381 287
SphI G_CATG'C
Cuts at: 0 202 1293 1551 2433
Size: 202 1091 258 882
Fragments arranged by size:
1091 882 258 202
StuI AGG'CCT
Cuts at: 0 141 1941 2276 2433
Size: 141 1800 335 157
Fragments arranged by size:
1800 335 157 141
StyI C'CwwG_G
Cuts at: 0 385 1284 1859 2433
Size: 385 899 575 574
Fragments arranged by size:
899 575 574 385
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 7 1869 1973 2433
Size: 7 1862 104 460
Fragments arranged by size:
1862 460 104 7
TatI w'GTAC_w
Cuts at: 0 1389 1992 2433
Size: 1389 603 441
Fragments arranged by size:
1389 603 441
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1874 1906 2275 2307 2433
Size: 1874 32 369 32 126
Fragments arranged by size:
1874 369 126 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1482 2433
Size: 1482 951
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1417 2433
Size: 1417 1016
XhoI C'TCGA_G
Cuts at: 0 1193 2433
Size: 1193 1240
Enzymes that do cut:
AcuI AfeI AlwNI ApoI AvaI BanI BanII BbsI
BbvCI BciVI BclI BglI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI BsrGI BstAPI BstYI BtgZI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII MfeI MmeI MslI
MspA1I NspI PasI PflMI PfoI PpuMI PshAI PspXI
PstI PvuI PvuII SacI SanDI SapI SbfI SfcI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BcgI BfrBI BglII
BlpI BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI
BsgI BsiWI BsmI BsmBI BspEI BspHI BsrBI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtsI
ClaI CspCI DraI DraIII EagI EcoRV FseI FspAI
HindIII HpaI KasI KpnI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PsiI PspOMI PsrI RsrII
SacII SalI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI XbaI XmaI
XmnI ZraI