Restriction map for
pFN21AE5758
(Linear) (Six-base) MAPSORT of: KIEE5758.seq Check: 4008 from: 1 to: 2337
KIEE5758.orf
With 182 enzymes: SgfI *
December 5, 2014 13:09 ..
Acc65I G'GTAC_C
Cuts at: 0 700 2337
Size: 700 1637
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 2176 2236 2337
Size: 153 2023 60 101
Fragments arranged by size:
2023 153 101 60
AfeI AGC'GCT
Cuts at: 0 343 1522 2337
Size: 343 1179 815
Fragments arranged by size:
1179 815 343
AleI CACnn'nnGTG
Cuts at: 0 2266 2337
Size: 2266 71
AlwNI CAG_nnn'CTG
Cuts at: 0 2101 2337
Size: 2101 236
ApoI r'AATT_y
Cuts at: 0 1407 1649 2194 2337
Size: 1407 242 545 143
Fragments arranged by size:
1407 545 242 143
AseI AT'TA_AT
Cuts at: 0 509 1279 1939 2337
Size: 509 770 660 398
Fragments arranged by size:
770 660 509 398
BamHI G'GATC_C
Cuts at: 0 388 2337
Size: 388 1949
BanI G'GyrC_C
Cuts at: 0 151 700 1218 2337
Size: 151 549 518 1119
Fragments arranged by size:
1119 549 518 151
BanII G_rGCy'C
Cuts at: 0 1571 2337
Size: 1571 766
BbsI GAAGACnn'nnnn_
Cuts at: 0 1368 2316 2337
Size: 1368 948 21
Fragments arranged by size:
1368 948 21
BbvCI CC'TCA_GC
Cuts at: 0 1537 2337
Size: 1537 800
BclI T'GATC_A
Cuts at: 0 1083 1911 2337
Size: 1083 828 426
Fragments arranged by size:
1083 828 426
BfrBI ATG'CAT
Cuts at: 0 1234 1415 2337
Size: 1234 181 922
Fragments arranged by size:
1234 922 181
BglII A'GATC_T
Cuts at: 0 80 2337
Size: 80 2257
Bme1580I G_kGCm'C
Cuts at: 0 135 154 2337
Size: 135 19 2183
Fragments arranged by size:
2183 135 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1604 2150 2337
Size: 910 694 546 187
Fragments arranged by size:
910 694 546 187
Bpu10I CC'TnA_GC
Cuts at: 0 1537 2337
Size: 1537 800
BsaHI Gr'CG_yC
Cuts at: 0 353 2337
Size: 353 1984
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 866 896 941 971 1431 1461 2036 2066
Size: 866 30 45 30 460 30 575 30
Cuts at: 2066 2337
Size: 271
Fragments arranged by size:
866 575 460 271 45 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 385 965 1870 2337
Size: 385 580 905 467
Fragments arranged by size:
905 580 467 385
BseYI C'CCAG_C
Cuts at: 0 276 2337
Size: 276 2061
BsiHKAI G_wGCw'C
Cuts at: 0 627 1571 2337
Size: 627 944 766
Fragments arranged by size:
944 766 627
BsmI GAATG_Cn'
Cuts at: 0 492 2337
Size: 492 1845
BsmBI CGTCTCn'nnnn_
Cuts at: 0 788 958 2337
Size: 788 170 1379
Fragments arranged by size:
1379 788 170
Bsp1286I G_dGCh'C
Cuts at: 0 135 154 627 1571 2337
Size: 135 19 473 944 766
Fragments arranged by size:
944 766 473 135 19
BspHI T'CATG_A
Cuts at: 0 1841 2337
Size: 1841 496
BssSI C'ACGA_G
Cuts at: 0 848 2075 2337
Size: 848 1227 262
Fragments arranged by size:
1227 848 262
BstEII G'GTnAC_C
Cuts at: 0 2178 2187 2337
Size: 2178 9 150
Fragments arranged by size:
2178 150 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 304 2337
Size: 304 2033
BstYI r'GATC_y
Cuts at: 0 80 388 1155 2302 2337
Size: 80 308 767 1147 35
Fragments arranged by size:
1147 767 308 80 35
BtgI C'CryG_G
Cuts at: 0 636 2337
Size: 636 1701
DraI TTT'AAA
Cuts at: 0 1754 2337
Size: 1754 583
DraIII CAC_nnn'GTG
Cuts at: 0 853 2337
Size: 853 1484
EarI CTCTTCn'nnn_
Cuts at: 0 786 1679 2168 2337
Size: 786 893 489 169
Fragments arranged by size:
893 786 489 169
EcoICRI GAG'CTC
Cuts at: 0 1569 2337
Size: 1569 768
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 910 1604 2150 2176 2236 2337
Size: 153 757 694 546 26 60 101
Fragments arranged by size:
757 694 546 153 101 60 26
EcoO109I rG'GnC_Cy
Cuts at: 0 783 1089 2098 2337
Size: 783 306 1009 239
Fragments arranged by size:
1009 783 306 239
EcoRV GAT'ATC
Cuts at: 0 444 2337
Size: 444 1893
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 183 408 754 1333 2337
Size: 183 225 346 579 1004
Fragments arranged by size:
1004 579 346 225 183
HaeII r_GCGC'y
Cuts at: 0 345 1269 1524 2337
Size: 345 924 255 813
Fragments arranged by size:
924 813 345 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 72 104 727 759 941 973 2337
Size: 72 32 623 32 182 32 1364
Fragments arranged by size:
1364 623 182 72 32 32 32
HincII GTy'rAC
Cuts at: 0 48 524 2337
Size: 48 476 1813
Fragments arranged by size:
1813 476 48
HpaI GTT'AAC
Cuts at: 0 48 2337
Size: 48 2289
KpnI G_GTAC'C
Cuts at: 0 704 2337
Size: 704 1633
MfeI C'AATT_G
Cuts at: 0 983 2337
Size: 983 1354
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1884 2337
Size: 1884 453
MslI CAynn'nnrTG
Cuts at: 0 302 2266 2337
Size: 302 1964 71
Fragments arranged by size:
1964 302 71
MspA1I CmG'CkG
Cuts at: 0 757 1393 2337
Size: 757 636 944
Fragments arranged by size:
944 757 636
NcoI C'CATG_G
Cuts at: 0 636 2337
Size: 636 1701
NsiI A_TGCA'T
Cuts at: 0 1236 1417 2337
Size: 1236 181 920
Fragments arranged by size:
1236 920 181
PpuMI rG'GwC_Cy
Cuts at: 0 1089 2098 2337
Size: 1089 1009 239
Fragments arranged by size:
1089 1009 239
PstI C_TGCA'G
Cuts at: 0 1289 1557 2337
Size: 1289 268 780
Fragments arranged by size:
1289 780 268
PvuII CAG'CTG
Cuts at: 0 757 1393 2337
Size: 757 636 944
Fragments arranged by size:
944 757 636
SacI G_AGCT'C
Cuts at: 0 1571 2337
Size: 1571 766
SapI GCTCTTCn'nnn_
Cuts at: 0 1679 2337
Size: 1679 658
SexAI A'CCwGG_T
Cuts at: 0 1734 2337
Size: 1734 603
SfcI C'TryA_G
Cuts at: 0 1285 1553 2337
Size: 1285 268 784
Fragments arranged by size:
1285 784 268
SpeI A'CTAG_T
Cuts at: 0 1633 2337
Size: 1633 704
StyI C'CwwG_G
Cuts at: 0 114 527 636 786 2183 2337
Size: 114 413 109 150 1397 154
Fragments arranged by size:
1397 413 154 150 114 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 839 866 871 898 1431 1463 2036 2068
Size: 839 27 5 27 533 32 573 32
Cuts at: 2068 2337
Size: 269
Fragments arranged by size:
839 573 533 269 32 32 27 27
5
XbaI T'CTAG_A
Cuts at: 0 2305 2337
Size: 2305 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 598 2337
Size: 598 1739
XmnI GAAnn'nnTTC
Cuts at: 0 166 2337
Size: 166 2171
Enzymes that do cut:
Acc65I AcuI AfeI AleI AlwNI ApoI AseI BamHI
BanI BanII BbsI BbvCI BclI BfrBI BglII Bme1580I
BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BssSI BstEII BstXI BstYI BtgI
DraI DraIII EarI EcoICRI Eco57MI EcoO109I EcoRV FalI
HaeII Hin4I HincII HpaI KpnI MfeI MmeI MslI
MspA1I NcoI NsiI PpuMI PstI PvuII SacI SapI
SexAI SfcI SpeI StyI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BbeI BcgI BciVI BglI BlpI BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsgI
BsiEI BsiWI BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DrdI EaeI EagI EciI EcoNI EcoRI FseI
FspI FspAI HindIII KasI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SphI SrfI SspI StuI SwaI TaqII TatI
Tth111I XhoI XmaI ZraI