Restriction map for
pFN21AE5753
(Linear) (Six-base) MAPSORT of: KIEE5753.seq Check: 8533 from: 1 to: 2175
KIEE5753.orf
With 182 enzymes: SgfI *
December 5, 2014 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 48 2175
Size: 48 2127
AccI GT'mk_AC
Cuts at: 0 146 2175
Size: 146 2029
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1228 1843 2175
Size: 1228 615 332
Fragments arranged by size:
1228 615 332
AflII C'TTAA_G
Cuts at: 0 1084 2175
Size: 1084 1091
AflIII A'CryG_T
Cuts at: 0 1138 2175
Size: 1138 1037
AleI CACnn'nnGTG
Cuts at: 0 198 2175
Size: 198 1977
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 323 355 1995 2027 2175
Size: 323 32 1640 32 148
Fragments arranged by size:
1640 323 148 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1318 1563 1644 1987 2175
Size: 1318 245 81 343 188
Fragments arranged by size:
1318 343 245 188 81
ApoI r'AATT_y
Cuts at: 0 213 438 1977 2175
Size: 213 225 1539 198
Fragments arranged by size:
1539 225 213 198
AvrII C'CTAG_G
Cuts at: 0 420 1099 2175
Size: 420 679 1076
Fragments arranged by size:
1076 679 420
BamHI G'GATC_C
Cuts at: 0 354 2175
Size: 354 1821
BanI G'GyrC_C
Cuts at: 0 1095 1295 1469 1749 2175
Size: 1095 200 174 280 426
Fragments arranged by size:
1095 426 280 200 174
BanII G_rGCy'C
Cuts at: 0 1604 2175
Size: 1604 571
BbsI GAAGACnn'nnnn_
Cuts at: 0 1234 1942 2175
Size: 1234 708 233
Fragments arranged by size:
1234 708 233
BciVI GTATCCnnnnn_n'
Cuts at: 0 1278 2175
Size: 1278 897
BglI GCCn_nnn'nGGC
Cuts at: 0 557 1313 2175
Size: 557 756 862
Fragments arranged by size:
862 756 557
BglII A'GATC_T
Cuts at: 0 112 2175
Size: 112 2063
BlpI GC'TnA_GC
Cuts at: 0 449 662 2175
Size: 449 213 1513
Fragments arranged by size:
1513 449 213
Bme1580I G_kGCm'C
Cuts at: 0 1098 1472 1752 2175
Size: 1098 374 280 423
Fragments arranged by size:
1098 423 374 280
BmrI ACTGGGnnnn_n'
Cuts at: 0 101 2175
Size: 101 2074
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 78 2175
Size: 78 2097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 853 1713 1784 1902 2067 2175
Size: 59 794 860 71 118 165 108
Fragments arranged by size:
860 794 165 118 108 71 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 1238 2175
Size: 311 927 937
Fragments arranged by size:
937 927 311
BsaI GGTCTCn'nnnn_
Cuts at: 0 604 1201 1369 1405 1756 2175
Size: 604 597 168 36 351 419
Fragments arranged by size:
604 597 419 351 168 36
BsaAI yAC'GTr
Cuts at: 0 1139 2175
Size: 1139 1036
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1213 1243 2175
Size: 1213 30 932
Fragments arranged by size:
1213 932 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 622 1350 1476 1516 2175
Size: 622 728 126 40 659
Fragments arranged by size:
728 659 622 126 40
BseYI C'CCAG_C
Cuts at: 0 454 631 2175
Size: 454 177 1544
Fragments arranged by size:
1544 454 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 2175
Size: 553 1622
BsmI GAATG_Cn'
Cuts at: 0 166 1434 2175
Size: 166 1268 741
Fragments arranged by size:
1268 741 166
Bsp1286I G_dGCh'C
Cuts at: 0 1098 1472 1604 1752 2175
Size: 1098 374 132 148 423
Fragments arranged by size:
1098 423 374 148 132
BspHI T'CATG_A
Cuts at: 0 1125 2175
Size: 1125 1050
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 48 484 2175
Size: 48 436 1691
Fragments arranged by size:
1691 436 48
BsrDI GCAATG_nn'
Cuts at: 0 320 2175
Size: 320 1855
BstXI CCAn_nnnn'nTGG
Cuts at: 0 857 1360 1777 2175
Size: 857 503 417 398
Fragments arranged by size:
857 503 417 398
BstYI r'GATC_y
Cuts at: 0 112 354 1002 1983 2175
Size: 112 242 648 981 192
Fragments arranged by size:
981 648 242 192 112
Bsu36I CC'TnA_GG
Cuts at: 0 1963 2175
Size: 1963 212
BtgI C'CryG_G
Cuts at: 0 808 1589 2175
Size: 808 781 586
Fragments arranged by size:
808 781 586
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1708 2175
Size: 1708 467
BtsI GCAGTG_nn'
Cuts at: 0 609 1826 2175
Size: 609 1217 349
Fragments arranged by size:
1217 609 349
DraI TTT'AAA
Cuts at: 0 587 2175
Size: 587 1588
EaeI y'GGCC_r
Cuts at: 0 805 2175
Size: 805 1370
EarI CTCTTCn'nnn_
Cuts at: 0 309 756 857 1494 2013 2127 2175
Size: 309 447 101 637 519 114 48
Fragments arranged by size:
637 519 447 309 114 101 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 853 1228 1713 1784 1843 1902 2067
Size: 59 794 375 485 71 59 59 165
Cuts at: 2067 2175
Size: 108
Fragments arranged by size:
794 485 375 165 108 71 59 59
59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 399 1103 1991 2175
Size: 399 704 888 184
Fragments arranged by size:
888 704 399 184
EcoO109I rG'GnC_Cy
Cuts at: 0 1854 1997 2175
Size: 1854 143 178
Fragments arranged by size:
1854 178 143
EcoRI G'AATT_C
Cuts at: 0 213 1977 2175
Size: 213 1764 198
Fragments arranged by size:
1764 213 198
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 958 979 990 1011 2126 2158 2175
Size: 958 21 11 21 1115 32 17
Fragments arranged by size:
1115 958 32 21 21 17 11
HindIII A'AGCT_T
Cuts at: 0 124 2175
Size: 124 2051
MscI TGG'CCA
Cuts at: 0 807 2175
Size: 807 1368
MslI CAynn'nnrTG
Cuts at: 0 198 1139 2175
Size: 198 941 1036
Fragments arranged by size:
1036 941 198
MspA1I CmG'CkG
Cuts at: 0 336 2175
Size: 336 1839
NcoI C'CATG_G
Cuts at: 0 808 1589 2175
Size: 808 781 586
Fragments arranged by size:
808 781 586
PfoI T'CCnGG_A
Cuts at: 0 1879 2175
Size: 1879 296
PpuMI rG'GwC_Cy
Cuts at: 0 1997 2175
Size: 1997 178
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1407 1439 2175
Size: 1407 32 736
Fragments arranged by size:
1407 736 32
PstI C_TGCA'G
Cuts at: 0 1323 2162 2175
Size: 1323 839 13
Fragments arranged by size:
1323 839 13
PvuII CAG'CTG
Cuts at: 0 336 2175
Size: 336 1839
SanDI GG'GwC_CC
Cuts at: 0 1997 2175
Size: 1997 178
ScaI AGT'ACT
Cuts at: 0 1017 2175
Size: 1017 1158
SfcI C'TryA_G
Cuts at: 0 426 615 1319 2158 2175
Size: 426 189 704 839 17
Fragments arranged by size:
839 704 426 189 17
SmlI C'TyrA_G
Cuts at: 0 326 1084 1253 2175
Size: 326 758 169 922
Fragments arranged by size:
922 758 326 169
StyI C'CwwG_G
Cuts at: 0 420 555 808 1099 1589 1674 2175
Size: 420 135 253 291 490 85 501
Fragments arranged by size:
501 490 420 291 253 135 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1041 1313 1519 2175
Size: 1041 272 206 656
Fragments arranged by size:
1041 656 272 206
TatI w'GTAC_w
Cuts at: 0 703 1015 1685 2175
Size: 703 312 670 490
Fragments arranged by size:
703 670 490 312
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 669 701 1462 1494 1861 1893 2175
Size: 669 32 761 32 367 32 282
Fragments arranged by size:
761 669 367 282 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2042 2175
Size: 2042 133
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AleI AloI AlwNI
ApoI AvrII BamHI BanI BanII BbsI BciVI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspHI
BspMI BsrDI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI Hin4I
HindIII MscI MslI MspA1I NcoI PfoI PpuMI PsrI
PstI PvuII SanDI ScaI SfcI SmlI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AlfI ApaI
ApaLI AscI AseI AsiSI AvaI BaeI BbeI BbvCI
BcgI BclI BfrBI BmgBI BmtI Bpu10I BsaBI BsaHI
BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoRV FalI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PvuI
RsrII SacI SacII SalI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI