Restriction map for
pFN21AE5751
(Linear) (Six-base) MAPSORT of: KIEE5751.seq Check: 8545 from: 1 to: 2124
KIEE5751.orf
With 182 enzymes: SgfI *
December 5, 2014 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1125 2124
Size: 1125 999
AccI GT'mk_AC
Cuts at: 0 269 2124
Size: 269 1855
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1718 1837 2124
Size: 1718 119 287
Fragments arranged by size:
1718 287 119
AhdI GACnn_n'nnGTC
Cuts at: 0 286 2124
Size: 286 1838
AlwNI CAG_nnn'CTG
Cuts at: 0 1342 2124
Size: 1342 782
ApaI G_GGCC'C
Cuts at: 0 1398 2124
Size: 1398 726
ApaLI G'TGCA_C
Cuts at: 0 358 2124
Size: 358 1766
ApoI r'AATT_y
Cuts at: 0 126 569 956 1645 1810 2124
Size: 126 443 387 689 165 314
Fragments arranged by size:
689 443 387 314 165 126
AseI AT'TA_AT
Cuts at: 0 308 509 2124
Size: 308 201 1615
Fragments arranged by size:
1615 308 201
AvaI C'yCGr_G
Cuts at: 0 400 2124
Size: 400 1724
BanI G'GyrC_C
Cuts at: 0 337 2124
Size: 337 1787
BanII G_rGCy'C
Cuts at: 0 213 904 1398 1490 2124
Size: 213 691 494 92 634
Fragments arranged by size:
691 634 494 213 92
BbsI GAAGACnn'nnnn_
Cuts at: 0 1155 1782 2124
Size: 1155 627 342
Fragments arranged by size:
1155 627 342
BclI T'GATC_A
Cuts at: 0 1001 2124
Size: 1001 1123
BfrBI ATG'CAT
Cuts at: 0 1276 2124
Size: 1276 848
BglI GCCn_nnn'nGGC
Cuts at: 0 1108 1996 2124
Size: 1108 888 128
Fragments arranged by size:
1108 888 128
Bme1580I G_kGCm'C
Cuts at: 0 362 1398 2124
Size: 362 1036 726
Fragments arranged by size:
1036 726 362
BmrI ACTGGGnnnn_n'
Cuts at: 0 798 2124
Size: 798 1326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 888 1256 2124
Size: 428 460 368 868
Fragments arranged by size:
868 460 428 368
Bpu10I CC'TnA_GC
Cuts at: 0 40 2124
Size: 40 2084
BsaAI yAC'GTr
Cuts at: 0 363 2124
Size: 363 1761
BsaBI GATnn'nnATC
Cuts at: 0 1000 2124
Size: 1000 1124
BsaHI Gr'CG_yC
Cuts at: 0 196 2124
Size: 196 1928
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1229 1259 2124
Size: 1229 30 865
Fragments arranged by size:
1229 865 30
BseYI C'CCAG_C
Cuts at: 0 137 231 2022 2124
Size: 137 94 1791 102
Fragments arranged by size:
1791 137 102 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1153 2124
Size: 1153 971
BsiHKAI G_wGCw'C
Cuts at: 0 213 362 779 1490 1901 2124
Size: 213 149 417 711 411 223
Fragments arranged by size:
711 417 411 223 213 149
BsmI GAATG_Cn'
Cuts at: 0 101 2124
Size: 101 2023
Bsp1286I G_dGCh'C
Cuts at: 0 213 362 779 904 1398 1490 1901 2124
Size: 213 149 417 125 494 92 411 223
Fragments arranged by size:
494 417 411 223 213 149 125 92
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1125 2124
Size: 1125 999
BsrBI CCG'CTC
Cuts at: 0 813 2124
Size: 813 1311
BsrDI GCAATG_nn'
Cuts at: 0 1040 2124
Size: 1040 1084
BsrGI T'GTAC_A
Cuts at: 0 665 2124
Size: 665 1459
BssSI C'ACGA_G
Cuts at: 0 225 2124
Size: 225 1899
BstAPI GCAn_nnn'nTGC
Cuts at: 0 366 2124
Size: 366 1758
BstXI CCAn_nnnn'nTGG
Cuts at: 0 878 2124
Size: 878 1246
BtgI C'CryG_G
Cuts at: 0 1106 1874 2124
Size: 1106 768 250
Fragments arranged by size:
1106 768 250
BtsI GCAGTG_nn'
Cuts at: 0 663 913 1340 2124
Size: 663 250 427 784
Fragments arranged by size:
784 663 427 250
ClaI AT'CG_AT
Cuts at: 0 1145 2124
Size: 1145 979
DraIII CAC_nnn'GTG
Cuts at: 0 366 2124
Size: 366 1758
DrdI GACnn_nn'nnGTC
Cuts at: 0 252 2124
Size: 252 1872
EaeI y'GGCC_r
Cuts at: 0 707 2124
Size: 707 1417
EarI CTCTTCn'nnn_
Cuts at: 0 449 1160 1235 2124
Size: 449 711 75 889
Fragments arranged by size:
889 711 449 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1595 2124
Size: 1595 529
EcoICRI GAG'CTC
Cuts at: 0 211 1488 2124
Size: 211 1277 636
Fragments arranged by size:
1277 636 211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 888 1256 1718 1837 2124
Size: 428 460 368 462 119 287
Fragments arranged by size:
462 460 428 368 287 119
EcoO109I rG'GnC_Cy
Cuts at: 0 1395 2124
Size: 1395 729
EcoRI G'AATT_C
Cuts at: 0 569 1810 2124
Size: 569 1241 314
Fragments arranged by size:
1241 569 314
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 200 232 272 304 1322 1354
Size: 136 32 32 32 40 32 1018 32
Cuts at: 1354 2022 2054 2124
Size: 668 32 70
Fragments arranged by size:
1018 668 136 70 40 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1618 1737 2124
Size: 1618 119 387
Fragments arranged by size:
1618 387 119
HindIII A'AGCT_T
Cuts at: 0 584 2124
Size: 584 1540
HpaI GTT'AAC
Cuts at: 0 1737 2124
Size: 1737 387
MfeI C'AATT_G
Cuts at: 0 275 2124
Size: 275 1849
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1859 2124
Size: 1859 265
MscI TGG'CCA
Cuts at: 0 709 2124
Size: 709 1415
MslI CAynn'nnrTG
Cuts at: 0 329 544 1501 1894 2124
Size: 329 215 957 393 230
Fragments arranged by size:
957 393 329 230 215
MspA1I CmG'CkG
Cuts at: 0 137 1691 2026 2124
Size: 137 1554 335 98
Fragments arranged by size:
1554 335 137 98
NcoI C'CATG_G
Cuts at: 0 1106 2124
Size: 1106 1018
NsiI A_TGCA'T
Cuts at: 0 1278 2124
Size: 1278 846
PfoI T'CCnGG_A
Cuts at: 0 934 1233 1326 2124
Size: 934 299 93 798
Fragments arranged by size:
934 798 299 93
PmlI CAC'GTG
Cuts at: 0 363 2124
Size: 363 1761
PspOMI G'GGCC_C
Cuts at: 0 1394 2124
Size: 1394 730
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 658 690 2124
Size: 658 32 1434
Fragments arranged by size:
1434 658 32
PstI C_TGCA'G
Cuts at: 0 920 2124
Size: 920 1204
PvuII CAG'CTG
Cuts at: 0 137 1691 2124
Size: 137 1554 433
Fragments arranged by size:
1554 433 137
SacI G_AGCT'C
Cuts at: 0 213 1490 2124
Size: 213 1277 634
Fragments arranged by size:
1277 634 213
SexAI A'CCwGG_T
Cuts at: 0 1409 2124
Size: 1409 715
SfcI C'TryA_G
Cuts at: 0 629 916 2124
Size: 629 287 1208
Fragments arranged by size:
1208 629 287
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1108 2124
Size: 1108 1016
StyI C'CwwG_G
Cuts at: 0 651 1106 2124
Size: 651 455 1018
Fragments arranged by size:
1018 651 455
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 558 2124
Size: 558 1566
TatI w'GTAC_w
Cuts at: 0 116 665 1943 2124
Size: 116 549 1278 181
Fragments arranged by size:
1278 549 181 116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1229 1261 2124
Size: 1229 32 863
Fragments arranged by size:
1229 863 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 331 498 1113 1134 2124
Size: 331 167 615 21 990
Fragments arranged by size:
990 615 331 167 21
Enzymes that do cut:
AarI AccI AcuI AhdI AlwNI ApaI ApaLI ApoI
AseI AvaI BanI BanII BbsI BclI BfrBI BglI
Bme1580I BmrI BpmI Bpu10I BsaAI BsaBI BsaHI BsaXI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrGI BssSI BstAPI BstXI BtgI BtsI ClaI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI
MspA1I NcoI NsiI PfoI PmlI PspOMI PsrI PstI
PvuII SacI SexAI SfcI SfiI StyI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI AscI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BglII BlpI BmgBI BmtI BplI
BpuEI BsaI BsaWI BseRI BsiEI BsiWI BsmBI BspEI
BspHI BsrFI BssHII BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI CspCI DraI EagI EcoNI EcoRV FalI FseI
FspI FspAI HaeII KasI KpnI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI
XmnI ZraI