Restriction map for
pFN21AE5749
(Linear) (Six-base) MAPSORT of: KIEE5749.seq Check: 1714 from: 1 to: 2019
KIEE5749.orf
With 182 enzymes: SgfI *
December 5, 2014 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1488 2019
Size: 1488 531
AccI GT'mk_AC
Cuts at: 0 1348 2019
Size: 1348 671
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1340 1552 2019
Size: 1340 212 467
Fragments arranged by size:
1340 467 212
AflIII A'CryG_T
Cuts at: 0 1568 2019
Size: 1568 451
AhdI GACnn_n'nnGTC
Cuts at: 0 43 1316 2019
Size: 43 1273 703
Fragments arranged by size:
1273 703 43
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 631 2019
Size: 631 1388
AlwNI CAG_nnn'CTG
Cuts at: 0 1363 1603 1828 2019
Size: 1363 240 225 191
Fragments arranged by size:
1363 240 225 191
ApaI G_GGCC'C
Cuts at: 0 1706 1957 2019
Size: 1706 251 62
Fragments arranged by size:
1706 251 62
ApoI r'AATT_y
Cuts at: 0 152 2019
Size: 152 1867
AvaI C'yCGr_G
Cuts at: 0 236 275 430 835 1049 1424 2019
Size: 236 39 155 405 214 375 595
Fragments arranged by size:
595 405 375 236 214 155 39
BamHI G'GATC_C
Cuts at: 0 1413 1536 2019
Size: 1413 123 483
Fragments arranged by size:
1413 483 123
BanI G'GyrC_C
Cuts at: 0 742 1045 1631 2019
Size: 742 303 586 388
Fragments arranged by size:
742 586 388 303
BanII G_rGCy'C
Cuts at: 0 533 1243 1388 1586 1706 1957 1976 2019
Size: 533 710 145 198 120 251 19 43
Fragments arranged by size:
710 533 251 198 145 120 43 19
BbeI G_GCGC'C
Cuts at: 0 1635 2019
Size: 1635 384
BbsI GAAGACnn'nnnn_
Cuts at: 0 110 1670 1910 2019
Size: 110 1560 240 109
Fragments arranged by size:
1560 240 110 109
BfrBI ATG'CAT
Cuts at: 0 591 2019
Size: 591 1428
Bme1580I G_kGCm'C
Cuts at: 0 431 896 1706 1957 2019
Size: 431 465 810 251 62
Fragments arranged by size:
810 465 431 251 62
BmgBI CAC'GTC
Cuts at: 0 1571 2019
Size: 1571 448
BmrI ACTGGGnnnn_n'
Cuts at: 0 1463 2019
Size: 1463 556
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1260 2019
Size: 1260 759
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 376 1102 2019
Size: 167 209 726 917
Fragments arranged by size:
917 726 209 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 2019
Size: 886 1133
BsaI GGTCTCn'nnnn_
Cuts at: 0 796 1805 2019
Size: 796 1009 214
Fragments arranged by size:
1009 796 214
BsaHI Gr'CG_yC
Cuts at: 0 1632 2019
Size: 1632 387
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 706 1030 1233 1236 1239 1396 2019
Size: 706 324 203 3 3 157 623
Fragments arranged by size:
706 623 324 203 157 3 3
BseYI C'CCAG_C
Cuts at: 0 579 1167 1333 1396 1617 2019
Size: 579 588 166 63 221 402
Fragments arranged by size:
588 579 402 221 166 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 792 2019
Size: 792 1227
BsiEI CG_ry'CG
Cuts at: 0 1450 2019
Size: 1450 569
BsiHKAI G_wGCw'C
Cuts at: 0 533 1243 1976 2019
Size: 533 710 733 43
Fragments arranged by size:
733 710 533 43
Bsp1286I G_dGCh'C
Cuts at: 0 431 533 896 1243 1388 1586 1706 1957
Size: 431 102 363 347 145 198 120 251
Cuts at: 1957 1976 2019
Size: 19 43
Fragments arranged by size:
431 363 347 251 198 145 120 102
43 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 814 1109 1488 2019
Size: 814 295 379 531
Fragments arranged by size:
814 531 379 295
BsrBI CCG'CTC
Cuts at: 0 1640 2019
Size: 1640 379
BsrDI GCAATG_nn'
Cuts at: 0 639 1071 2019
Size: 639 432 948
Fragments arranged by size:
948 639 432
BssSI C'ACGA_G
Cuts at: 0 1847 2019
Size: 1847 172
BstEII G'GTnAC_C
Cuts at: 0 414 777 2019
Size: 414 363 1242
Fragments arranged by size:
1242 414 363
BstXI CCAn_nnnn'nTGG
Cuts at: 0 792 903 2019
Size: 792 111 1116
Fragments arranged by size:
1116 792 111
BstYI r'GATC_y
Cuts at: 0 435 1413 1536 2019
Size: 435 978 123 483
Fragments arranged by size:
978 483 435 123
Bsu36I CC'TnA_GG
Cuts at: 0 1949 2019
Size: 1949 70
BtgI C'CryG_G
Cuts at: 0 419 1293 1299 1779 2019
Size: 419 874 6 480 240
Fragments arranged by size:
874 480 419 240 6
DrdI GACnn_nn'nnGTC
Cuts at: 0 1672 1748 2019
Size: 1672 76 271
Fragments arranged by size:
1672 271 76
EaeI y'GGCC_r
Cuts at: 0 367 1296 1302 1447 2019
Size: 367 929 6 145 572
Fragments arranged by size:
929 572 367 145 6
EagI C'GGCC_G
Cuts at: 0 1447 2019
Size: 1447 572
EarI CTCTTCn'nnn_
Cuts at: 0 538 794 1369 2019
Size: 538 256 575 650
Fragments arranged by size:
650 575 538 256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1968 2019
Size: 1968 51
EcoICRI GAG'CTC
Cuts at: 0 531 1241 1974 2019
Size: 531 710 733 45
Fragments arranged by size:
733 710 531 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 376 1102 1340 1552 2019
Size: 167 209 726 238 212 467
Fragments arranged by size:
726 467 238 212 209 167
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1105 2019
Size: 1105 914
EcoO109I rG'GnC_Cy
Cuts at: 0 68 711 1471 1953 1954 2019
Size: 68 643 760 482 1 65
Fragments arranged by size:
760 643 482 68 65 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1927 2019
Size: 1927 92
FspI TGC'GCA
Cuts at: 0 607 2019
Size: 607 1412
HaeII r_GCGC'y
Cuts at: 0 1635 2019
Size: 1635 384
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 61 1043 1075 1225 1257 1302 1334
Size: 29 32 982 32 150 32 45 32
Cuts at: 1334 1351 1383 1828 1860 2019
Size: 17 32 445 32 159
Fragments arranged by size:
982 445 159 150 45 32 32 32
32 32 32 29 17
HincII GTy'rAC
Cuts at: 0 545 2019
Size: 545 1474
HindIII A'AGCT_T
Cuts at: 0 1942 2019
Size: 1942 77
KasI G'GCGC_C
Cuts at: 0 1631 2019
Size: 1631 388
MfeI C'AATT_G
Cuts at: 0 728 2019
Size: 728 1291
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 2019
Size: 86 1933
MslI CAynn'nnrTG
Cuts at: 0 424 2019
Size: 424 1595
MspA1I CmG'CkG
Cuts at: 0 187 1202 2019
Size: 187 1015 817
Fragments arranged by size:
1015 817 187
NarI GG'CG_CC
Cuts at: 0 1632 2019
Size: 1632 387
NcoI C'CATG_G
Cuts at: 0 419 2019
Size: 419 1600
NsiI A_TGCA'T
Cuts at: 0 593 2019
Size: 593 1426
PasI CC'CwG_GG
Cuts at: 0 489 1010 2019
Size: 489 521 1009
Fragments arranged by size:
1009 521 489
PfoI T'CCnGG_A
Cuts at: 0 941 2019
Size: 941 1078
PpuMI rG'GwC_Cy
Cuts at: 0 711 1471 2019
Size: 711 760 548
Fragments arranged by size:
760 711 548
PspOMI G'GGCC_C
Cuts at: 0 1702 1953 2019
Size: 1702 251 66
Fragments arranged by size:
1702 251 66
PstI C_TGCA'G
Cuts at: 0 1106 2019
Size: 1106 913
PvuII CAG'CTG
Cuts at: 0 187 2019
Size: 187 1832
SacI G_AGCT'C
Cuts at: 0 533 1243 1976 2019
Size: 533 710 733 43
Fragments arranged by size:
733 710 533 43
SanDI GG'GwC_CC
Cuts at: 0 1471 2019
Size: 1471 548
SapI GCTCTTCn'nnn_
Cuts at: 0 538 2019
Size: 538 1481
SbfI CC_TGCA'GG
Cuts at: 0 1106 2019
Size: 1106 913
SexAI A'CCwGG_T
Cuts at: 0 902 2019
Size: 902 1117
SfcI C'TryA_G
Cuts at: 0 1102 1349 1554 2019
Size: 1102 247 205 465
Fragments arranged by size:
1102 465 247 205
SfoI GGC'GCC
Cuts at: 0 1633 2019
Size: 1633 386
SmaI CCC'GGG
Cuts at: 0 277 2019
Size: 277 1742
SmlI C'TyrA_G
Cuts at: 0 236 865 2019
Size: 236 629 1154
Fragments arranged by size:
1154 629 236
StuI AGG'CCT
Cuts at: 0 1688 2019
Size: 1688 331
StyI C'CwwG_G
Cuts at: 0 419 1985 2019
Size: 419 1566 34
Fragments arranged by size:
1566 419 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 571 784 2019
Size: 571 213 1235
Fragments arranged by size:
1235 571 213
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 726 758 2019
Size: 726 32 1261
Fragments arranged by size:
1261 726 32
Tth111I GACn'n_nGTC
Cuts at: 0 109 1365 1933 2019
Size: 109 1256 568 86
Fragments arranged by size:
1256 568 109 86
XhoI C'TCGA_G
Cuts at: 0 236 2019
Size: 236 1783
XmaI C'CCGG_G
Cuts at: 0 275 2019
Size: 275 1744
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlfI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbeI BbsI BfrBI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaHI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BssSI BstEII BstXI BstYI Bsu36I BtgI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HincII HindIII KasI MfeI
MmeI MslI MspA1I NarI NcoI NsiI PasI PfoI
PpuMI PspOMI PstI PvuII SacI SanDI SapI SbfI
SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII
TstI Tth111I XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BcgI
BciVI BclI BglI BglII BlpI BmtI Bpu10I BsaAI
BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI
BsrFI BsrGI BssHII BstAPI BstBI BstZ17I BtgZI BtsI
ClaI CspCI DraI DraIII EcoRI EcoRV FseI FspAI
HpaI KpnI MluI MscI NaeI NdeI NgoMIV NheI
NotI NruI NspI PacI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TatI XbaI XcmI XmnI ZraI