Restriction map for
pFN21AE5747
(Linear) (Six-base) MAPSORT of: KIEE5747.seq Check: 3889 from: 1 to: 1914
KIEE5747.orf
With 182 enzymes: SgfI *
December 5, 2014 13:09 ..
AatII G_ACGT'C
Cuts at: 0 259 1914
Size: 259 1655
AccI GT'mk_AC
Cuts at: 0 1511 1914
Size: 1511 403
Acc65I G'GTAC_C
Cuts at: 0 695 1914
Size: 695 1219
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1011 1914
Size: 1011 903
AflIII A'CryG_T
Cuts at: 0 665 1914
Size: 665 1249
AleI CACnn'nnGTG
Cuts at: 0 1672 1914
Size: 1672 242
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 463 495 1914
Size: 463 32 1419
Fragments arranged by size:
1419 463 32
AlwNI CAG_nnn'CTG
Cuts at: 0 585 1197 1317 1361 1458 1592 1914
Size: 585 612 120 44 97 134 322
Fragments arranged by size:
612 585 322 134 120 97 44
ApaI G_GGCC'C
Cuts at: 0 1889 1914
Size: 1889 25
ApaLI G'TGCA_C
Cuts at: 0 361 1078 1914
Size: 361 717 836
Fragments arranged by size:
836 717 361
AvaI C'yCGr_G
Cuts at: 0 594 1087 1914
Size: 594 493 827
Fragments arranged by size:
827 594 493
BanI G'GyrC_C
Cuts at: 0 131 211 332 695 1016 1274 1545 1560
Size: 131 80 121 363 321 258 271 15
Cuts at: 1560 1914
Size: 354
Fragments arranged by size:
363 354 321 271 258 131 121 80
15
BanII G_rGCy'C
Cuts at: 0 55 285 314 548 1096 1523 1637 1811
Size: 55 230 29 234 548 427 114 174
Cuts at: 1811 1889 1914
Size: 78 25
Fragments arranged by size:
548 427 234 230 174 114 78 55
29 25
BbeI G_GCGC'C
Cuts at: 0 215 1549 1914
Size: 215 1334 365
Fragments arranged by size:
1334 365 215
BbvCI CC'TCA_GC
Cuts at: 0 499 1914
Size: 499 1415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1642 1676 1914
Size: 1642 34 238
Fragments arranged by size:
1642 238 34
BglI GCCn_nnn'nGGC
Cuts at: 0 87 596 767 1100 1738 1815 1914
Size: 87 509 171 333 638 77 99
Fragments arranged by size:
638 509 333 171 99 87 77
BlpI GC'TnA_GC
Cuts at: 0 5 586 884 1914
Size: 5 581 298 1030
Fragments arranged by size:
1030 581 298 5
Bme1580I G_kGCm'C
Cuts at: 0 134 365 1019 1082 1889 1914
Size: 134 231 654 63 807 25
Fragments arranged by size:
807 654 231 134 63 25
BmgBI CAC'GTC
Cuts at: 0 415 668 1793 1914
Size: 415 253 1125 121
Fragments arranged by size:
1125 415 253 121
BmrI ACTGGGnnnn_n'
Cuts at: 0 1400 1914
Size: 1400 514
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 535 1230 1914
Size: 535 695 684
Fragments arranged by size:
695 684 535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 463 1339 1383 1651 1660 1914
Size: 46 417 876 44 268 9 254
Fragments arranged by size:
876 417 268 254 46 44 9
Bpu10I CC'TnA_GC
Cuts at: 0 499 1914
Size: 499 1415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1914
Size: 232 1682
BsaI GGTCTCn'nnnn_
Cuts at: 0 1201 1315 1914
Size: 1201 114 599
Fragments arranged by size:
1201 599 114
BsaHI Gr'CG_yC
Cuts at: 0 79 212 256 872 1546 1741 1914
Size: 79 133 44 616 674 195 173
Fragments arranged by size:
674 616 195 173 133 79 44
BsaWI w'CCGG_w
Cuts at: 0 426 824 1914
Size: 426 398 1090
Fragments arranged by size:
1090 426 398
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 272 302 463 493 1138 1168 1914
Size: 272 30 161 30 645 30 746
Fragments arranged by size:
746 645 272 161 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 304 1497 1555 1914
Size: 304 1193 58 359
Fragments arranged by size:
1193 359 304 58
BseYI C'CCAG_C
Cuts at: 0 306 1810 1877 1882 1889 1914
Size: 306 1504 67 5 7 25
Fragments arranged by size:
1504 306 67 25 7 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 1092 1914
Size: 174 918 822
Fragments arranged by size:
918 822 174
BsiEI CG_ry'CG
Cuts at: 0 421 866 993 1686 1914
Size: 421 445 127 693 228
Fragments arranged by size:
693 445 421 228 127
BsiHKAI G_wGCw'C
Cuts at: 0 11 365 1082 1523 1637 1914
Size: 11 354 717 441 114 277
Fragments arranged by size:
717 441 354 277 114 11
BsmI GAATG_Cn'
Cuts at: 0 1375 1914
Size: 1375 539
BsmBI CGTCTCn'nnnn_
Cuts at: 0 360 1914
Size: 360 1554
Bsp1286I G_dGCh'C
Cuts at: 0 11 55 134 285 314 365 548 1019
Size: 11 44 79 151 29 51 183 471
Cuts at: 1019 1082 1096 1523 1637 1811 1889 1914
Size: 63 14 427 114 174 78 25
Fragments arranged by size:
471 427 183 174 151 114 79 78
63 51 44 29 25 14 11
BspEI T'CCGG_A
Cuts at: 0 824 1914
Size: 824 1090
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1219 1914
Size: 1219 695
BsrBI CCG'CTC
Cuts at: 0 1532 1914
Size: 1532 382
BsrFI r'CCGG_y
Cuts at: 0 967 1082 1114 1242 1328 1688 1914
Size: 967 115 32 128 86 360 226
Fragments arranged by size:
967 360 226 128 115 86 32
BsrGI T'GTAC_A
Cuts at: 0 485 1914
Size: 485 1429
BssSI C'ACGA_G
Cuts at: 0 541 1914
Size: 541 1373
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1592 1914
Size: 1592 322
BstEII G'GTnAC_C
Cuts at: 0 390 1914
Size: 390 1524
BstXI CCAn_nnnn'nTGG
Cuts at: 0 222 1914
Size: 222 1692
BstYI r'GATC_y
Cuts at: 0 569 1914
Size: 569 1345
Bsu36I CC'TnA_GG
Cuts at: 0 922 1734 1914
Size: 922 812 180
Fragments arranged by size:
922 812 180
BtgI C'CryG_G
Cuts at: 0 207 234 734 753 903 1580 1828 1914
Size: 207 27 500 19 150 677 248 86
Fragments arranged by size:
677 500 248 207 150 86 27 19
BtsI GCAGTG_nn'
Cuts at: 0 1104 1290 1914
Size: 1104 186 624
Fragments arranged by size:
1104 624 186
DraIII CAC_nnn'GTG
Cuts at: 0 398 711 1914
Size: 398 313 1203
Fragments arranged by size:
1203 398 313
EaeI y'GGCC_r
Cuts at: 0 350 418 768 965 990 1036 1101 1436
Size: 350 68 350 197 25 46 65 335
Cuts at: 1436 1493 1577 1683 1914
Size: 57 84 106 231
Fragments arranged by size:
350 350 335 231 197 106 84 68
65 57 46 25
EagI C'GGCC_G
Cuts at: 0 418 990 1683 1914
Size: 418 572 693 231
Fragments arranged by size:
693 572 418 231
EarI CTCTTCn'nnn_
Cuts at: 0 1865 1914
Size: 1865 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 208 424 513 1401 1914
Size: 208 216 89 888 513
Fragments arranged by size:
888 513 216 208 89
EcoICRI GAG'CTC
Cuts at: 0 1521 1635 1914
Size: 1521 114 279
Fragments arranged by size:
1521 279 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 463 1011 1339 1383 1651 1660 1914
Size: 46 417 548 328 44 268 9 254
Fragments arranged by size:
548 417 328 268 254 46 44 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1161 1309 1733 1914
Size: 1161 148 424 181
Fragments arranged by size:
1161 424 181 148
EcoO109I rG'GnC_Cy
Cuts at: 0 1314 1358 1394 1610 1886 1914
Size: 1314 44 36 216 276 28
Fragments arranged by size:
1314 276 216 44 36 28
EcoRV GAT'ATC
Cuts at: 0 717 1914
Size: 717 1197
HaeII r_GCGC'y
Cuts at: 0 215 891 1549 1914
Size: 215 676 658 365
Fragments arranged by size:
676 658 365 215
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 272 304 1914
Size: 272 32 1610
Fragments arranged by size:
1610 272 32
HincII GTy'rAC
Cuts at: 0 495 1914
Size: 495 1419
KasI G'GCGC_C
Cuts at: 0 211 1545 1914
Size: 211 1334 369
Fragments arranged by size:
1334 369 211
KpnI G_GTAC'C
Cuts at: 0 699 1914
Size: 699 1215
MscI TGG'CCA
Cuts at: 0 1438 1495 1914
Size: 1438 57 419
Fragments arranged by size:
1438 419 57
MslI CAynn'nnrTG
Cuts at: 0 1672 1914
Size: 1672 242
MspA1I CmG'CkG
Cuts at: 0 236 306 387 736 854 1197 1349 1423
Size: 236 70 81 349 118 343 152 74
Cuts at: 1423 1569 1814 1914
Size: 146 245 100
Fragments arranged by size:
349 343 245 236 152 146 118 100
81 74 70
NaeI GCC'GGC
Cuts at: 0 969 1116 1244 1690 1914
Size: 969 147 128 446 224
Fragments arranged by size:
969 446 224 147 128
NarI GG'CG_CC
Cuts at: 0 212 1546 1914
Size: 212 1334 368
Fragments arranged by size:
1334 368 212
NcoI C'CATG_G
Cuts at: 0 1580 1914
Size: 1580 334
NgoMIV G'CCGG_C
Cuts at: 0 967 1114 1242 1688 1914
Size: 967 147 128 446 226
Fragments arranged by size:
967 446 226 147 128
NotI GC'GGCC_GC
Cuts at: 0 1683 1914
Size: 1683 231
PasI CC'CwG_GG
Cuts at: 0 276 1914
Size: 276 1638
PflMI CCAn_nnn'nTGG
Cuts at: 0 711 1317 1914
Size: 711 606 597
Fragments arranged by size:
711 606 597
PfoI T'CCnGG_A
Cuts at: 0 167 686 1628 1637 1914
Size: 167 519 942 9 277
Fragments arranged by size:
942 519 277 167 9
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 1914
Size: 463 32 1419
Fragments arranged by size:
1419 463 32
PpuMI rG'GwC_Cy
Cuts at: 0 1314 1394 1914
Size: 1314 80 520
Fragments arranged by size:
1314 520 80
PspOMI G'GGCC_C
Cuts at: 0 1885 1914
Size: 1885 29
PstI C_TGCA'G
Cuts at: 0 72 581 790 1264 1366 1597 1785 1914
Size: 72 509 209 474 102 231 188 129
Fragments arranged by size:
509 474 231 209 188 129 102 72
PvuII CAG'CTG
Cuts at: 0 306 854 1197 1423 1569 1914
Size: 306 548 343 226 146 345
Fragments arranged by size:
548 345 343 306 226 146
SacI G_AGCT'C
Cuts at: 0 1523 1637 1914
Size: 1523 114 277
Fragments arranged by size:
1523 277 114
SacII CC_GC'GG
Cuts at: 0 237 737 1914
Size: 237 500 1177
Fragments arranged by size:
1177 500 237
SbfI CC_TGCA'GG
Cuts at: 0 1366 1785 1914
Size: 1366 419 129
Fragments arranged by size:
1366 419 129
ScaI AGT'ACT
Cuts at: 0 1258 1914
Size: 1258 656
SfcI C'TryA_G
Cuts at: 0 68 577 786 1260 1362 1593 1781 1914
Size: 68 509 209 474 102 231 188 133
Fragments arranged by size:
509 474 231 209 188 133 102 68
SfoI GGC'GCC
Cuts at: 0 213 1547 1914
Size: 213 1334 367
Fragments arranged by size:
1334 367 213
SgrAI Cr'CCGG_yG
Cuts at: 0 1114 1914
Size: 1114 800
SmlI C'TyrA_G
Cuts at: 0 247 1897 1914
Size: 247 1650 17
Fragments arranged by size:
1650 247 17
StuI AGG'CCT
Cuts at: 0 1171 1914
Size: 1171 743
StyI C'CwwG_G
Cuts at: 0 122 1580 1914
Size: 122 1458 334
Fragments arranged by size:
1458 334 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 135 916 1914
Size: 135 781 998
Fragments arranged by size:
998 781 135
TatI w'GTAC_w
Cuts at: 0 485 1256 1553 1914
Size: 485 771 297 361
Fragments arranged by size:
771 485 361 297
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1138 1170 1914
Size: 1138 32 744
Fragments arranged by size:
1138 744 32
Tth111I GACn'n_nGTC
Cuts at: 0 255 1914
Size: 255 1659
ZraI GAC'GTC
Cuts at: 0 257 1914
Size: 257 1657
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AleI AloI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII KasI
KpnI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI PasI PflMI PfoI PpiI PpuMI PspOMI PstI
PvuII SacI SacII SbfI ScaI SfcI SfoI SgrAI
SmlI StuI StyI TaqII TatI TstI Tth111I ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AlfI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI
BclI BfrBI BglII BmtI BsaAI BsaBI BsiWI BspHI
BsrDI BssHII BstBI BstZ17I BtgZI ClaI CspCI DraI
DrdI EcoRI FalI FseI FspI FspAI HindIII HpaI
MfeI MluI MmeI NdeI NheI NruI NsiI NspI
PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SanDI SapI SexAI SfiI SgfI
SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XcmI XhoI XmaI XmnI