Restriction map for
pFN21AE5745
(Linear) (Six-base) MAPSORT of: KIEE5745.seq Check: 3862 from: 1 to: 1920
KIEE5745.orf
With 182 enzymes: SgfI *
December 5, 2014 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 790 1920
Size: 790 1130
Acc65I G'GTAC_C
Cuts at: 0 431 1920
Size: 431 1489
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1920
Size: 295 1625
AflIII A'CryG_T
Cuts at: 0 15 218 1360 1920
Size: 15 203 1142 560
Fragments arranged by size:
1142 560 203 15
AleI CACnn'nnGTG
Cuts at: 0 328 1920
Size: 328 1592
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1774 1920
Size: 1774 146
AlwNI CAG_nnn'CTG
Cuts at: 0 447 1920
Size: 447 1473
ApaI G_GGCC'C
Cuts at: 0 661 1122 1920
Size: 661 461 798
Fragments arranged by size:
798 661 461
ApaLI G'TGCA_C
Cuts at: 0 623 1920
Size: 623 1297
ApoI r'AATT_y
Cuts at: 0 1204 1420 1504 1920
Size: 1204 216 84 416
Fragments arranged by size:
1204 416 216 84
AvaI C'yCGr_G
Cuts at: 0 488 1243 1920
Size: 488 755 677
Fragments arranged by size:
755 677 488
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 423 456 1920
Size: 423 33 1464
Fragments arranged by size:
1464 423 33
BamHI G'GATC_C
Cuts at: 0 1268 1920
Size: 1268 652
BanI G'GyrC_C
Cuts at: 0 431 1255 1920
Size: 431 824 665
Fragments arranged by size:
824 665 431
BanII G_rGCy'C
Cuts at: 0 487 661 1031 1122 1400 1920
Size: 487 174 370 91 278 520
Fragments arranged by size:
520 487 370 278 174 91
BbsI GAAGACnn'nnnn_
Cuts at: 0 904 1920
Size: 904 1016
BfrBI ATG'CAT
Cuts at: 0 1815 1920
Size: 1815 105
BlpI GC'TnA_GC
Cuts at: 0 811 1920
Size: 811 1109
Bme1580I G_kGCm'C
Cuts at: 0 627 661 707 1122 1258 1920
Size: 627 34 46 415 136 662
Fragments arranged by size:
662 627 415 136 46 34
BmgBI CAC'GTC
Cuts at: 0 337 375 1920
Size: 337 38 1545
Fragments arranged by size:
1545 337 38
BmtI G_CTAG'C
Cuts at: 0 196 1920
Size: 196 1724
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 501 1920
Size: 501 1419
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 553 610 787 805 1045 1920
Size: 526 27 57 177 18 240 875
Fragments arranged by size:
875 526 240 177 57 27 18
Bpu10I CC'TnA_GC
Cuts at: 0 1836 1920
Size: 1836 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 703 969 1920
Size: 100 603 266 951
Fragments arranged by size:
951 603 266 100
BsaI GGTCTCn'nnnn_
Cuts at: 0 1774 1920
Size: 1774 146
BsaAI yAC'GTr
Cuts at: 0 129 219 246 1920
Size: 129 90 27 1674
Fragments arranged by size:
1674 129 90 27
BsaBI GATnn'nnATC
Cuts at: 0 1273 1920
Size: 1273 647
BsaHI Gr'CG_yC
Cuts at: 0 438 752 1920
Size: 438 314 1168
Fragments arranged by size:
1168 438 314
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 617 647 778 808 1747 1777 1920
Size: 617 30 131 30 939 30 143
Fragments arranged by size:
939 617 143 131 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 190 386 538 562 607 1755 1920
Size: 190 196 152 24 45 1148 165
Fragments arranged by size:
1148 196 190 165 152 45 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1066 1129 1207 1252 1636 1920
Size: 622 444 63 78 45 384 284
Fragments arranged by size:
622 444 384 284 78 63 45
BsiEI CG_ry'CG
Cuts at: 0 141 1920
Size: 141 1779
BsiHKAI G_wGCw'C
Cuts at: 0 124 251 487 627 812 1031 1920
Size: 124 127 236 140 185 219 889
Fragments arranged by size:
889 236 219 185 140 127 124
BsmBI CGTCTCn'nnnn_
Cuts at: 0 172 327 1920
Size: 172 155 1593
Fragments arranged by size:
1593 172 155
Bsp1286I G_dGCh'C
Cuts at: 0 124 251 487 627 661 707 812 1031
Size: 124 127 236 140 34 46 105 219
Cuts at: 1031 1122 1258 1400 1920
Size: 91 136 142 520
Fragments arranged by size:
520 236 219 142 140 136 127 124
105 91 46 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 790 1920
Size: 790 1130
BsrBI CCG'CTC
Cuts at: 0 978 1920
Size: 978 942
BsrFI r'CCGG_y
Cuts at: 0 327 434 735 1920
Size: 327 107 301 1185
Fragments arranged by size:
1185 327 301 107
BssHII G'CGCG_C
Cuts at: 0 390 1920
Size: 390 1530
BstAPI GCAn_nnn'nTGC
Cuts at: 0 782 1230 1920
Size: 782 448 690
Fragments arranged by size:
782 690 448
BstYI r'GATC_y
Cuts at: 0 210 1268 1920
Size: 210 1058 652
Fragments arranged by size:
1058 652 210
Bsu36I CC'TnA_GG
Cuts at: 0 398 1637 1920
Size: 398 1239 283
Fragments arranged by size:
1239 398 283
BtgI C'CryG_G
Cuts at: 0 197 356 740 935 1176 1733 1920
Size: 197 159 384 195 241 557 187
Fragments arranged by size:
557 384 241 197 195 187 159
BtsI GCAGTG_nn'
Cuts at: 0 1437 1811 1920
Size: 1437 374 109
Fragments arranged by size:
1437 374 109
DraIII CAC_nnn'GTG
Cuts at: 0 1152 1230 1920
Size: 1152 78 690
Fragments arranged by size:
1152 690 78
DrdI GACnn_nn'nnGTC
Cuts at: 0 351 1920
Size: 351 1569
EaeI y'GGCC_r
Cuts at: 0 138 452 599 737 932 1499 1677 1920
Size: 138 314 147 138 195 567 178 243
Fragments arranged by size:
567 314 243 195 178 147 138 138
EagI C'GGCC_G
Cuts at: 0 138 1920
Size: 138 1782
EarI CTCTTCn'nnn_
Cuts at: 0 475 1096 1920
Size: 475 621 824
Fragments arranged by size:
824 621 475
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 357 1920
Size: 357 1563
EcoICRI GAG'CTC
Cuts at: 0 485 1029 1920
Size: 485 544 891
Fragments arranged by size:
891 544 485
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 526 553 610 787 805 1045 1920
Size: 295 231 27 57 177 18 240 875
Fragments arranged by size:
875 295 240 231 177 57 27 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 235 1920
Size: 235 1685
EcoO109I rG'GnC_Cy
Cuts at: 0 270 528 1004 1119 1407 1521 1920
Size: 270 258 476 115 288 114 399
Fragments arranged by size:
476 399 288 270 258 115 114
EcoRI G'AATT_C
Cuts at: 0 1420 1920
Size: 1420 500
FspI TGC'GCA
Cuts at: 0 766 1920
Size: 766 1154
HaeII r_GCGC'y
Cuts at: 0 447 1920
Size: 447 1473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1747 1779 1920
Size: 1747 32 141
Fragments arranged by size:
1747 141 32
KpnI G_GTAC'C
Cuts at: 0 435 1920
Size: 435 1485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 997 1920
Size: 997 923
MscI TGG'CCA
Cuts at: 0 454 601 1501 1920
Size: 454 147 900 419
Fragments arranged by size:
900 454 419 147
MslI CAynn'nnrTG
Cuts at: 0 328 1492 1920
Size: 328 1164 428
Fragments arranged by size:
1164 428 328
MspA1I CmG'CkG
Cuts at: 0 513 1178 1344 1920
Size: 513 665 166 576
Fragments arranged by size:
665 576 513 166
NaeI GCC'GGC
Cuts at: 0 737 1920
Size: 737 1183
NcoI C'CATG_G
Cuts at: 0 197 356 740 1733 1920
Size: 197 159 384 993 187
Fragments arranged by size:
993 384 197 187 159
NgoMIV G'CCGG_C
Cuts at: 0 735 1920
Size: 735 1185
NheI G'CTAG_C
Cuts at: 0 192 1920
Size: 192 1728
NotI GC'GGCC_GC
Cuts at: 0 138 1920
Size: 138 1782
NsiI A_TGCA'T
Cuts at: 0 1817 1920
Size: 1817 103
NspI r_CATG'y
Cuts at: 0 19 974 1364 1761 1920
Size: 19 955 390 397 159
Fragments arranged by size:
955 397 390 159 19
PciI A'CATG_T
Cuts at: 0 15 1360 1920
Size: 15 1345 560
Fragments arranged by size:
1345 560 15
PfoI T'CCnGG_A
Cuts at: 0 206 1108 1920
Size: 206 902 812
Fragments arranged by size:
902 812 206
PmlI CAC'GTG
Cuts at: 0 129 1920
Size: 129 1791
PpuMI rG'GwC_Cy
Cuts at: 0 270 1407 1920
Size: 270 1137 513
Fragments arranged by size:
1137 513 270
PspOMI G'GGCC_C
Cuts at: 0 657 1118 1920
Size: 657 461 802
Fragments arranged by size:
802 657 461
PspXI vC'TCGA_Gb
Cuts at: 0 1243 1920
Size: 1243 677
PstI C_TGCA'G
Cuts at: 0 1753 1920
Size: 1753 167
PvuII CAG'CTG
Cuts at: 0 1344 1920
Size: 1344 576
RsrII CG'GwC_CG
Cuts at: 0 303 1920
Size: 303 1617
SacI G_AGCT'C
Cuts at: 0 487 1031 1920
Size: 487 544 889
Fragments arranged by size:
889 544 487
SacII CC_GC'GG
Cuts at: 0 1179 1920
Size: 1179 741
SapI GCTCTTCn'nnn_
Cuts at: 0 475 1920
Size: 475 1445
SexAI A'CCwGG_T
Cuts at: 0 1721 1920
Size: 1721 199
SfcI C'TryA_G
Cuts at: 0 1626 1749 1920
Size: 1626 123 171
Fragments arranged by size:
1626 171 123
SgrAI Cr'CCGG_yG
Cuts at: 0 327 1920
Size: 327 1593
SmaI CCC'GGG
Cuts at: 0 490 1920
Size: 490 1430
SmlI C'TyrA_G
Cuts at: 0 115 718 948 1243 1920
Size: 115 603 230 295 677
Fragments arranged by size:
677 603 295 230 115
SphI G_CATG'C
Cuts at: 0 974 1920
Size: 974 946
StyI C'CwwG_G
Cuts at: 0 197 356 740 1733 1920
Size: 197 159 384 993 187
Fragments arranged by size:
993 384 197 187 159
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 617 649 1253 1285 1920
Size: 617 32 604 32 635
Fragments arranged by size:
635 617 604 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 376 1920
Size: 376 1544
XhoI C'TCGA_G
Cuts at: 0 1243 1920
Size: 1243 677
XmaI C'CCGG_G
Cuts at: 0 488 1920
Size: 488 1432
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BaeI BamHI BanI BanII BbsI
BfrBI BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII
BstAPI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I KpnI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NheI NotI NsiI NspI PciI
PfoI PmlI PpuMI PspOMI PspXI PstI PvuII RsrII
SacI SacII SapI SexAI SfcI SgrAI SmaI SmlI
SphI StyI TstI Tth111I XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AloI
AscI AseI AsiSI AvrII BbeI BbvCI BcgI BciVI
BclI BglI BglII BmrI BsaWI BseYI BsiWI BsmI
BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstXI
BstZ17I BtgZI ClaI CspCI DraI EcoRV FalI FseI
FspAI HincII HindIII HpaI KasI MfeI MluI NarI
NdeI NruI PacI PasI PflMI PmeI PpiI PshAI
PsiI PsrI PvuI SalI SanDI SbfI ScaI SfiI
SfoI SgfI SnaBI SpeI SrfI SspI StuI SwaI
TaqII TatI XbaI XcmI XmnI ZraI