Restriction map for
pFN21AE5740
(Linear) (Six-base) MAPSORT of: KIEE5740.seq Check: 4392 from: 1 to: 2106
KIEE5740.orf
With 182 enzymes: SgfI *
December 5, 2014 13:09 ..
AatII G_ACGT'C
Cuts at: 0 1760 2106
Size: 1760 346
AccI GT'mk_AC
Cuts at: 0 505 2106
Size: 505 1601
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 2106
Size: 808 1298
AfeI AGC'GCT
Cuts at: 0 1185 2106
Size: 1185 921
AflII C'TTAA_G
Cuts at: 0 1948 2106
Size: 1948 158
AgeI A'CCGG_T
Cuts at: 0 342 2106
Size: 342 1764
AleI CACnn'nnGTG
Cuts at: 0 1876 2106
Size: 1876 230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1063 2106
Size: 1063 1043
ApaI G_GGCC'C
Cuts at: 0 361 1781 2106
Size: 361 1420 325
Fragments arranged by size:
1420 361 325
ApoI r'AATT_y
Cuts at: 0 588 639 1021 1435 1550 2106
Size: 588 51 382 414 115 556
Fragments arranged by size:
588 556 414 382 115 51
AvaI C'yCGr_G
Cuts at: 0 9 109 283 312 2106
Size: 9 100 174 29 1794
Fragments arranged by size:
1794 174 100 29 9
BamHI G'GATC_C
Cuts at: 0 916 2106
Size: 916 1190
BanI G'GyrC_C
Cuts at: 0 137 2106
Size: 137 1969
BanII G_rGCy'C
Cuts at: 0 198 361 1247 1781 2106
Size: 198 163 886 534 325
Fragments arranged by size:
886 534 325 198 163
BbeI G_GCGC'C
Cuts at: 0 141 2106
Size: 141 1965
BbsI GAAGACnn'nnnn_
Cuts at: 0 970 2085 2106
Size: 970 1115 21
Fragments arranged by size:
1115 970 21
BbvCI CC'TCA_GC
Cuts at: 0 85 2106
Size: 85 2021
BciVI GTATCCnnnnn_n'
Cuts at: 0 535 1759 2106
Size: 535 1224 347
Fragments arranged by size:
1224 535 347
BglI GCCn_nnn'nGGC
Cuts at: 0 169 437 2106
Size: 169 268 1669
Fragments arranged by size:
1669 268 169
BglII A'GATC_T
Cuts at: 0 1560 2106
Size: 1560 546
Bme1580I G_kGCm'C
Cuts at: 0 361 1781 1883 2106
Size: 361 1420 102 223
Fragments arranged by size:
1420 361 223 102
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 767 2106
Size: 767 1339
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 2036 2106
Size: 204 1832 70
Fragments arranged by size:
1832 204 70
Bpu10I CC'TnA_GC
Cuts at: 0 85 1582 1894 2106
Size: 85 1497 312 212
Fragments arranged by size:
1497 312 212 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 801 1593 2106
Size: 540 261 792 513
Fragments arranged by size:
792 540 513 261
BsaI GGTCTCn'nnnn_
Cuts at: 0 222 2106
Size: 222 1884
BsaAI yAC'GTr
Cuts at: 0 1290 2106
Size: 1290 816
BsaHI Gr'CG_yC
Cuts at: 0 138 1757 2106
Size: 138 1619 349
Fragments arranged by size:
1619 349 138
BsaWI w'CCGG_w
Cuts at: 0 342 1670 2106
Size: 342 1328 436
Fragments arranged by size:
1328 436 342
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 322 638 2065 2106
Size: 322 316 1427 41
Fragments arranged by size:
1427 322 316 41
BseYI C'CCAG_C
Cuts at: 0 74 247 1282 1727 2106
Size: 74 173 1035 445 379
Fragments arranged by size:
1035 445 379 173 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 2106
Size: 466 1640
BsiEI CG_ry'CG
Cuts at: 0 132 173 366 389 2106
Size: 132 41 193 23 1717
Fragments arranged by size:
1717 193 132 41 23
BsmI GAATG_Cn'
Cuts at: 0 1510 2106
Size: 1510 596
Bsp1286I G_dGCh'C
Cuts at: 0 198 361 1247 1781 1883 2106
Size: 198 163 886 534 102 223
Fragments arranged by size:
886 534 223 198 163 102
BspEI T'CCGG_A
Cuts at: 0 1670 2106
Size: 1670 436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 438 2106
Size: 438 1668
BsrBI CCG'CTC
Cuts at: 0 1304 2106
Size: 1304 802
BsrFI r'CCGG_y
Cuts at: 0 92 104 172 323 342 384 2106
Size: 92 12 68 151 19 42 1722
Fragments arranged by size:
1722 151 92 68 42 19 12
BsrGI T'GTAC_A
Cuts at: 0 1367 2106
Size: 1367 739
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1420 2106
Size: 1420 686
BstXI CCAn_nnnn'nTGG
Cuts at: 0 928 1290 2106
Size: 928 362 816
Fragments arranged by size:
928 816 362
BstYI r'GATC_y
Cuts at: 0 413 916 1560 1745 2106
Size: 413 503 644 185 361
Fragments arranged by size:
644 503 413 361 185
BstZ17I GTA'TAC
Cuts at: 0 506 2106
Size: 506 1600
Bsu36I CC'TnA_GG
Cuts at: 0 1093 2048 2106
Size: 1093 955 58
Fragments arranged by size:
1093 955 58
BtgI C'CryG_G
Cuts at: 0 353 2106
Size: 353 1753
DraI TTT'AAA
Cuts at: 0 2007 2106
Size: 2007 99
DraIII CAC_nnn'GTG
Cuts at: 0 1942 2106
Size: 1942 164
EaeI y'GGCC_r
Cuts at: 0 170 363 438 2106
Size: 170 193 75 1668
Fragments arranged by size:
1668 193 170 75
EagI C'GGCC_G
Cuts at: 0 170 363 2106
Size: 170 193 1743
Fragments arranged by size:
1743 193 170
EarI CTCTTCn'nnn_
Cuts at: 0 795 851 1883 2106
Size: 795 56 1032 223
Fragments arranged by size:
1032 795 223 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 297 2106
Size: 19 278 1809
Fragments arranged by size:
1809 278 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 808 2036 2106
Size: 204 604 1228 70
Fragments arranged by size:
1228 604 204 70
EcoO109I rG'GnC_Cy
Cuts at: 0 358 1647 2106
Size: 358 1289 459
Fragments arranged by size:
1289 459 358
HaeII r_GCGC'y
Cuts at: 0 141 1187 2106
Size: 141 1046 919
Fragments arranged by size:
1046 919 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 481 513 1380 1412 1626 1658 2106
Size: 481 32 867 32 214 32 448
Fragments arranged by size:
867 481 448 214 32 32 32
HincII GTy'rAC
Cuts at: 0 830 938 990 1215 2106
Size: 830 108 52 225 891
Fragments arranged by size:
891 830 225 108 52
HpaI GTT'AAC
Cuts at: 0 830 2106
Size: 830 1276
KasI G'GCGC_C
Cuts at: 0 137 2106
Size: 137 1969
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 909 1899 2106
Size: 772 137 990 207
Fragments arranged by size:
990 772 207 137
MscI TGG'CCA
Cuts at: 0 440 2106
Size: 440 1666
MslI CAynn'nnrTG
Cuts at: 0 733 1685 1843 1876 2106
Size: 733 952 158 33 230
Fragments arranged by size:
952 733 230 158 33
MspA1I CmG'CkG
Cuts at: 0 1590 1727 2106
Size: 1590 137 379
Fragments arranged by size:
1590 379 137
NaeI GCC'GGC
Cuts at: 0 94 106 174 2106
Size: 94 12 68 1932
Fragments arranged by size:
1932 94 68 12
NarI GG'CG_CC
Cuts at: 0 138 2106
Size: 138 1968
NgoMIV G'CCGG_C
Cuts at: 0 92 104 172 2106
Size: 92 12 68 1934
Fragments arranged by size:
1934 92 68 12
PfoI T'CCnGG_A
Cuts at: 0 1748 2013 2106
Size: 1748 265 93
Fragments arranged by size:
1748 265 93
PmlI CAC'GTG
Cuts at: 0 1290 2106
Size: 1290 816
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 284 316 2106
Size: 284 32 1790
Fragments arranged by size:
1790 284 32
PpuMI rG'GwC_Cy
Cuts at: 0 1647 2106
Size: 1647 459
PspOMI G'GGCC_C
Cuts at: 0 357 1777 2106
Size: 357 1420 329
Fragments arranged by size:
1420 357 329
PspXI vC'TCGA_Gb
Cuts at: 0 312 2106
Size: 312 1794
PstI C_TGCA'G
Cuts at: 0 1595 2106
Size: 1595 511
PvuII CAG'CTG
Cuts at: 0 1590 1727 2106
Size: 1590 137 379
Fragments arranged by size:
1590 379 137
SapI GCTCTTCn'nnn_
Cuts at: 0 1883 2106
Size: 1883 223
SfcI C'TryA_G
Cuts at: 0 1591 1830 2106
Size: 1591 239 276
Fragments arranged by size:
1591 276 239
SfoI GGC'GCC
Cuts at: 0 139 2106
Size: 139 1967
SgrAI Cr'CCGG_yG
Cuts at: 0 342 2106
Size: 342 1764
SmlI C'TyrA_G
Cuts at: 0 312 519 780 1572 1948 2106
Size: 312 207 261 792 376 158
Fragments arranged by size:
792 376 312 261 207 158
SspI AAT'ATT
Cuts at: 0 772 1056 2106
Size: 772 284 1050
Fragments arranged by size:
1050 772 284
StuI AGG'CCT
Cuts at: 0 821 2106
Size: 821 1285
StyI C'CwwG_G
Cuts at: 0 471 2106
Size: 471 1635
TatI w'GTAC_w
Cuts at: 0 1367 2106
Size: 1367 739
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 405 437 1928 1960 2106
Size: 405 32 1491 32 146
Fragments arranged by size:
1491 405 146 32 32
XhoI C'TCGA_G
Cuts at: 0 312 2106
Size: 312 1794
XmnI GAAnn'nnTTC
Cuts at: 0 1067 2106
Size: 1067 1039
ZraI GAC'GTC
Cuts at: 0 1758 2106
Size: 1758 348
Enzymes that do cut:
AatII AccI AcuI AfeI AflII AgeI AleI AlfI
ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BglI BglII Bme1580I BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsmI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI
BstAPI BstXI BstYI BstZ17I Bsu36I BtgI DraI DraIII
EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I
HincII HpaI KasI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV PfoI PmlI PpiI PpuMI PspOMI PspXI
PstI PvuII SapI SfcI SfoI SgrAI SmlI SspI
StuI StyI TatI TstI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflIII AhdI AloI AlwNI ApaLI
AscI AseI AsiSI AvrII BaeI BcgI BclI BfrBI
BlpI BmgBI BmrI BmtI BsaBI BsaXI BsiHKAI BsiWI
BsmBI BspHI BsrDI BssHII BssSI BstBI BstEII BtgZI
BtsI ClaI CspCI DrdI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HindIII KpnI MfeI MluI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PshAI PsiI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SgfI SmaI SnaBI SpeI SphI SrfI SwaI
TaqII Tth111I XbaI XcmI XmaI