(Linear) (Six-base) MAPSORT of: KIEE5739.seq  Check: 2575  from: 1  to: 1827



KIEE5739.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:09  ..

AatII G_ACGT'C

Cuts at:      0      43    1827 
   Size:         43    1784
 
AclI AA'CG_TT

Cuts at:      0     483    1827 
   Size:        483    1344
 
ApoI r'AATT_y

Cuts at:      0     498     807    1022    1159    1259    1827 
   Size:        498     309     215     137     100     568

  Fragments arranged by size: 

                 568     498     309     215     137     100
 
AvrII C'CTAG_G

Cuts at:      0     493    1827 
   Size:        493    1334
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     335     368    1827 
   Size:        335      33    1459

  Fragments arranged by size: 

                1459     335      33
 
BanII G_rGCy'C

Cuts at:      0    1567    1827 
   Size:       1567     260
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     382    1827 
   Size:        382    1445
 
Bme1580I G_kGCm'C

Cuts at:      0    1405    1827 
   Size:       1405     422
 
BmtI G_CTAG'C

Cuts at:      0      92    1827 
   Size:         92    1735
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     761    1827 
   Size:        761    1066
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1450    1827 
   Size:       1450     377
 
BsaBI GATnn'nnATC

Cuts at:      0     470    1827 
   Size:        470    1357
 
BsaHI Gr'CG_yC

Cuts at:      0      40    1827 
   Size:         40    1787
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     200     224    1705    1827 
   Size:        200      24    1481     122

  Fragments arranged by size: 

                1481     200     122      24
 
BsiHKAI G_wGCw'C

Cuts at:      0     204    1827 
   Size:        204    1623
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      31    1827 
   Size:         31    1796
 
Bsp1286I G_dGCh'C

Cuts at:      0     204    1405    1567    1827 
   Size:        204    1201     162     260

  Fragments arranged by size: 

                1201     260     204     162
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      52    1827 
   Size:         52    1775
 
BsrDI GCAATG_nn'

Cuts at:      0     930    1827 
   Size:        930     897
 
BsrGI T'GTAC_A

Cuts at:      0    1231    1827 
   Size:       1231     596
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     196    1743    1827 
   Size:        196    1547      84

  Fragments arranged by size: 

                1547     196      84
 
BstYI r'GATC_y

Cuts at:      0     742    1827 
   Size:        742    1085
 
BtgI C'CryG_G

Cuts at:      0    1430    1827 
   Size:       1430     397
 
BtsI GCAGTG_nn'

Cuts at:      0     524    1827 
   Size:        524    1303
 
ClaI AT'CG_AT

Cuts at:      0     846    1827 
   Size:        846     981
 
DraI TTT'AAA

Cuts at:      0    1125    1827 
   Size:       1125     702
 
EarI CTCTTCn'nnn_

Cuts at:      0     985    1555    1827 
   Size:        985     570     272

  Fragments arranged by size: 

                 985     570     272
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     761    1827 
   Size:        761    1066
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     380     412     778     810    1746    1778    1827 
   Size:        380      32     366      32     936      32      49

  Fragments arranged by size: 

                 936     380     366      49      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1585    1827 
   Size:       1585     242
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     238     652    1827 
   Size:        137     101     414    1175

  Fragments arranged by size: 

                1175     414     137     101
 
MslI CAynn'nnrTG

Cuts at:      0     194     383     474    1827 
   Size:        194     189      91    1353

  Fragments arranged by size: 

                1353     194     189      91
 
MspA1I CmG'CkG

Cuts at:      0    1759    1827 
   Size:       1759      68
 
NcoI C'CATG_G

Cuts at:      0    1430    1827 
   Size:       1430     397
 
NdeI CA'TA_TG

Cuts at:      0     364    1769    1827 
   Size:        364    1405      58

  Fragments arranged by size: 

                1405     364      58
 
NheI G'CTAG_C

Cuts at:      0      88    1827 
   Size:         88    1739
 
NspI r_CATG'y

Cuts at:      0    1382    1827 
   Size:       1382     445
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1308    1340    1827 
   Size:       1308      32     487

  Fragments arranged by size: 

                1308     487      32
 
PstI C_TGCA'G

Cuts at:      0      63    1827 
   Size:         63    1764
 
PvuII CAG'CTG

Cuts at:      0    1759    1827 
   Size:       1759      68
 
SapI GCTCTTCn'nnn_

Cuts at:      0     985    1555    1827 
   Size:        985     570     272

  Fragments arranged by size: 

                 985     570     272
 
SexAI A'CCwGG_T

Cuts at:      0     355    1827 
   Size:        355    1472
 
SfcI C'TryA_G

Cuts at:      0      59     992    1827 
   Size:         59     933     835

  Fragments arranged by size: 

                 933     835      59
 
SphI G_CATG'C

Cuts at:      0    1382    1827 
   Size:       1382     445
 
SspI AAT'ATT

Cuts at:      0    1524    1827 
   Size:       1524     303
 
StyI C'CwwG_G

Cuts at:      0     493    1430    1827 
   Size:        493     937     397

  Fragments arranged by size: 

                 937     493     397
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1251    1827 
   Size:       1251     576
 
TatI w'GTAC_w

Cuts at:      0     872    1231    1361    1827 
   Size:        872     359     130     466

  Fragments arranged by size: 

                 872     466     359     130
 
XmnI GAAnn'nnTTC

Cuts at:      0    1587    1827 
   Size:       1587     240
 
ZraI GAC'GTC

Cuts at:      0      41    1827 
   Size:         41    1786
 

 Enzymes that do cut:

    AatII     AclI     ApoI    AvrII     BaeI    BanII    BciVI Bme1580I
     BmtI     BpmI     BsaI    BsaBI    BsaHI    BseRI  BsiHKAI    BsmBI
 Bsp1286I    BspMI    BsrDI    BsrGI    BstXI    BstYI     BtgI     BtsI
     ClaI     DraI     EarI  Eco57MI    Hin4I  HindIII     MmeI     MslI
   MspA1I     NcoI     NdeI     NheI     NspI     PsrI     PstI    PvuII
     SapI    SexAI     SfcI     SphI     SspI     StyI    TaqII     TatI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BplI   Bpu10I    BpuEI    BsaAI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI    CspCI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
   NgoMIV     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI