Restriction map for
pFN21AE5738
(Linear) (Six-base) MAPSORT of: KIEE5738.seq Check: 781 from: 1 to: 2109
KIEE5738.orf
With 182 enzymes: SgfI *
December 5, 2014 13:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 1922 2109
Size: 829 1093 187
Fragments arranged by size:
1093 829 187
AflIII A'CryG_T
Cuts at: 0 758 2109
Size: 758 1351
AgeI A'CCGG_T
Cuts at: 0 719 2109
Size: 719 1390
AleI CACnn'nnGTG
Cuts at: 0 1084 2109
Size: 1084 1025
ApoI r'AATT_y
Cuts at: 0 856 1047 1235 1858 2109
Size: 856 191 188 623 251
Fragments arranged by size:
856 623 251 191 188
AvaI C'yCGr_G
Cuts at: 0 572 2109
Size: 572 1537
BbsI GAAGACnn'nnnn_
Cuts at: 0 229 2109
Size: 229 1880
BbvCI CC'TCA_GC
Cuts at: 0 1057 1369 2109
Size: 1057 312 740
Fragments arranged by size:
1057 740 312
BciVI GTATCCnnnnn_n'
Cuts at: 0 1579 2109
Size: 1579 530
BfrBI ATG'CAT
Cuts at: 0 1065 1219 2109
Size: 1065 154 890
Fragments arranged by size:
1065 890 154
BglII A'GATC_T
Cuts at: 0 1779 2109
Size: 1779 330
BmrI ACTGGGnnnn_n'
Cuts at: 0 42 1318 1568 2109
Size: 42 1276 250 541
Fragments arranged by size:
1276 541 250 42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1791 2109
Size: 1791 318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1529 1661 1938 2109
Size: 1529 132 277 171
Fragments arranged by size:
1529 277 171 132
Bpu10I CC'TnA_GC
Cuts at: 0 1057 1369 2109
Size: 1057 312 740
Fragments arranged by size:
1057 740 312
BsaI GGTCTCn'nnnn_
Cuts at: 0 1949 2109
Size: 1949 160
BsaAI yAC'GTr
Cuts at: 0 759 1623 2109
Size: 759 864 486
Fragments arranged by size:
864 759 486
BsaBI GATnn'nnATC
Cuts at: 0 1567 2109
Size: 1567 542
BsaWI w'CCGG_w
Cuts at: 0 719 2109
Size: 719 1390
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 1244 2029 2109
Size: 91 1153 785 80
Fragments arranged by size:
1153 785 91 80
BseYI C'CCAG_C
Cuts at: 0 697 985 2109
Size: 697 288 1124
Fragments arranged by size:
1124 697 288
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 2109
Size: 662 1447
BsiHKAI G_wGCw'C
Cuts at: 0 1622 2109
Size: 1622 487
BsmI GAATG_Cn'
Cuts at: 0 422 1558 2109
Size: 422 1136 551
Fragments arranged by size:
1136 551 422
Bsp1286I G_dGCh'C
Cuts at: 0 1622 2109
Size: 1622 487
BspHI T'CATG_A
Cuts at: 0 432 2109
Size: 432 1677
BsrDI GCAATG_nn'
Cuts at: 0 287 747 980 1315 2109
Size: 287 460 233 335 794
Fragments arranged by size:
794 460 335 287 233
BsrFI r'CCGG_y
Cuts at: 0 719 2109
Size: 719 1390
BsrGI T'GTAC_A
Cuts at: 0 896 2109
Size: 896 1213
BstEII G'GTnAC_C
Cuts at: 0 394 2109
Size: 394 1715
BstYI r'GATC_y
Cuts at: 0 466 1779 2109
Size: 466 1313 330
Fragments arranged by size:
1313 466 330
BtgI C'CryG_G
Cuts at: 0 767 1469 2109
Size: 767 702 640
Fragments arranged by size:
767 702 640
BtsI GCAGTG_nn'
Cuts at: 0 278 420 872 1157 1974 2109
Size: 278 142 452 285 817 135
Fragments arranged by size:
817 452 285 278 142 135
DraI TTT'AAA
Cuts at: 0 1902 2109
Size: 1902 207
EarI CTCTTCn'nnn_
Cuts at: 0 261 805 1475 2006 2109
Size: 261 544 670 531 103
Fragments arranged by size:
670 544 531 261 103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 1529 1661 1922 1938 2109
Size: 829 700 132 261 16 171
Fragments arranged by size:
829 700 261 171 132 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1038 2109
Size: 1038 1071
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1862 2109
Size: 1862 247
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 555 587 1004 1036 1429 1461 1572 1604
Size: 555 32 417 32 393 32 111 32
Cuts at: 1604 2109
Size: 505
Fragments arranged by size:
555 505 417 393 111 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1659 2109
Size: 1659 450
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 2109
Size: 102 2007
MslI CAynn'nnrTG
Cuts at: 0 509 935 1070 1081 1084 1561 2109
Size: 509 426 135 11 3 477 548
Fragments arranged by size:
548 509 477 426 135 11 3
MspA1I CmG'CkG
Cuts at: 0 1164 1549 1698 2109
Size: 1164 385 149 411
Fragments arranged by size:
1164 411 385 149
NcoI C'CATG_G
Cuts at: 0 1469 2109
Size: 1469 640
NdeI CA'TA_TG
Cuts at: 0 803 2109
Size: 803 1306
NsiI A_TGCA'T
Cuts at: 0 1067 1221 2109
Size: 1067 154 888
Fragments arranged by size:
1067 888 154
NspI r_CATG'y
Cuts at: 0 413 892 1822 2109
Size: 413 479 930 287
Fragments arranged by size:
930 479 413 287
PasI CC'CwG_GG
Cuts at: 0 33 1532 2109
Size: 33 1499 577
Fragments arranged by size:
1499 577 33
PmlI CAC'GTG
Cuts at: 0 759 1623 2109
Size: 759 864 486
Fragments arranged by size:
864 759 486
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 728 760 2109
Size: 728 32 1349
Fragments arranged by size:
1349 728 32
PvuII CAG'CTG
Cuts at: 0 1164 1549 1698 2109
Size: 1164 385 149 411
Fragments arranged by size:
1164 411 385 149
SapI GCTCTTCn'nnn_
Cuts at: 0 261 2109
Size: 261 1848
SmlI C'TyrA_G
Cuts at: 0 572 2109
Size: 572 1537
SphI G_CATG'C
Cuts at: 0 413 1822 2109
Size: 413 1409 287
Fragments arranged by size:
1409 413 287
SspI AAT'ATT
Cuts at: 0 602 651 2109
Size: 602 49 1458
Fragments arranged by size:
1458 602 49
StuI AGG'CCT
Cuts at: 0 1914 1951 2109
Size: 1914 37 158
Fragments arranged by size:
1914 158 37
StyI C'CwwG_G
Cuts at: 0 1469 2109
Size: 1469 640
SwaI ATTT'AAAT
Cuts at: 0 1902 2109
Size: 1902 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 25 452 1448 2109
Size: 25 427 996 661
Fragments arranged by size:
996 661 427 25
TatI w'GTAC_w
Cuts at: 0 896 2109
Size: 896 1213
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1136 1168 2109
Size: 1136 32 941
Fragments arranged by size:
1136 941 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 307 2071 2109
Size: 307 1764 38
Fragments arranged by size:
1764 307 38
XhoI C'TCGA_G
Cuts at: 0 572 2109
Size: 572 1537
XmnI GAAnn'nnTTC
Cuts at: 0 65 2109
Size: 65 2044
Enzymes that do cut:
AcuI AflIII AgeI AleI ApoI AvaI BbsI BbvCI
BciVI BfrBI BglII BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaBI BsaWI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BsrDI BsrFI BsrGI BstEII BstYI BtgI
BtsI DraI EarI Eco57MI EcoNI FalI Hin4I HincII
MmeI MslI MspA1I NcoI NdeI NsiI NspI PasI
PmlI PpiI PvuII SapI SmlI SphI SspI StuI
StyI SwaI TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BanII BbeI BcgI BclI
BglI BlpI Bme1580I BmgBI BmtI BpuEI BsaHI BsaXI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BssHII BssSI
BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraIII DrdI EaeI EagI EciI EcoICRI EcoO109I EcoRI
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NgoMIV NheI
NotI NruI PacI PciI PflMI PfoI PmeI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
Tth111I XbaI XmaI ZraI