Restriction map for
pFN21AE5736
(Linear) (Six-base) MAPSORT of: KIEE5736.seq Check: 7970 from: 1 to: 2496
KIEE5736.orf
With 182 enzymes: SgfI *
December 5, 2014 13:08 ..
AccI GT'mk_AC
Cuts at: 0 2185 2370 2496
Size: 2185 185 126
Fragments arranged by size:
2185 185 126
AclI AA'CG_TT
Cuts at: 0 1313 2496
Size: 1313 1183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 1400 2495 2496
Size: 654 746 1095 1
Fragments arranged by size:
1095 746 654 1
AflIII A'CryG_T
Cuts at: 0 1015 1704 2496
Size: 1015 689 792
Fragments arranged by size:
1015 792 689
AleI CACnn'nnGTG
Cuts at: 0 685 781 1750 2496
Size: 685 96 969 746
Fragments arranged by size:
969 746 685 96
AlwNI CAG_nnn'CTG
Cuts at: 0 1573 2496
Size: 1573 923
ApaLI G'TGCA_C
Cuts at: 0 803 2042 2496
Size: 803 1239 454
Fragments arranged by size:
1239 803 454
ApoI r'AATT_y
Cuts at: 0 663 974 1457 2496
Size: 663 311 483 1039
Fragments arranged by size:
1039 663 483 311
AseI AT'TA_AT
Cuts at: 0 998 2496
Size: 998 1498
AvaI C'yCGr_G
Cuts at: 0 575 2496
Size: 575 1921
BamHI G'GATC_C
Cuts at: 0 1773 2496
Size: 1773 723
BanII G_rGCy'C
Cuts at: 0 1945 2496
Size: 1945 551
BbsI GAAGACnn'nnnn_
Cuts at: 0 25 1388 1925 2496
Size: 25 1363 537 571
Fragments arranged by size:
1363 571 537 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 431 2074 2108 2496
Size: 397 34 1643 34 388
Fragments arranged by size:
1643 397 388 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2336 2496
Size: 2336 160
BclI T'GATC_A
Cuts at: 0 518 1329 2079 2496
Size: 518 811 750 417
Fragments arranged by size:
811 750 518 417
BfrBI ATG'CAT
Cuts at: 0 913 2496
Size: 913 1583
BglII A'GATC_T
Cuts at: 0 265 1853 2496
Size: 265 1588 643
Fragments arranged by size:
1588 643 265
Bme1580I G_kGCm'C
Cuts at: 0 807 2046 2496
Size: 807 1239 450
Fragments arranged by size:
1239 807 450
BmrI ACTGGGnnnn_n'
Cuts at: 0 197 1072 1454 2154 2348 2496
Size: 197 875 382 700 194 148
Fragments arranged by size:
875 700 382 197 194 148
BmtI G_CTAG'C
Cuts at: 0 2002 2496
Size: 2002 494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 364 2496
Size: 265 99 2132
Fragments arranged by size:
2132 265 99
Bpu10I CC'TnA_GC
Cuts at: 0 1607 2496
Size: 1607 889
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1959 2450 2496
Size: 1959 491 46
Fragments arranged by size:
1959 491 46
BsaAI yAC'GTr
Cuts at: 0 879 1707 1842 2496
Size: 879 828 135 654
Fragments arranged by size:
879 828 654 135
BsaBI GATnn'nnATC
Cuts at: 0 502 1339 2078 2233 2496
Size: 502 837 739 155 263
Fragments arranged by size:
837 739 502 263 155
BsaHI Gr'CG_yC
Cuts at: 0 1494 2496
Size: 1494 1002
BsaWI w'CCGG_w
Cuts at: 0 1528 2496
Size: 1528 968
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2200 2230 2496
Size: 2200 30 266
Fragments arranged by size:
2200 266 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1235 2221 2496
Size: 1235 986 275
Fragments arranged by size:
1235 986 275
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1073 2496
Size: 1073 1423
BsiHKAI G_wGCw'C
Cuts at: 0 807 1945 2046 2466 2496
Size: 807 1138 101 420 30
Fragments arranged by size:
1138 807 420 101 30
Bsp1286I G_dGCh'C
Cuts at: 0 807 1945 2046 2466 2496
Size: 807 1138 101 420 30
Fragments arranged by size:
1138 807 420 101 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2317 2496
Size: 2317 179
BsrDI GCAATG_nn'
Cuts at: 0 1764 2496
Size: 1764 732
BstEII G'GTnAC_C
Cuts at: 0 2329 2496
Size: 2329 167
BstXI CCAn_nnnn'nTGG
Cuts at: 0 456 687 1846 2388 2496
Size: 456 231 1159 542 108
Fragments arranged by size:
1159 542 456 231 108
BstYI r'GATC_y
Cuts at: 0 265 583 1334 1512 1773 1853 2496
Size: 265 318 751 178 261 80 643
Fragments arranged by size:
751 643 318 265 261 178 80
BstZ17I GTA'TAC
Cuts at: 0 2371 2496
Size: 2371 125
BtgI C'CryG_G
Cuts at: 0 1166 2496
Size: 1166 1330
BtsI GCAGTG_nn'
Cuts at: 0 2130 2496
Size: 2130 366
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 713 748 2496
Size: 713 35 1748
Fragments arranged by size:
1748 713 35
EaeI y'GGCC_r
Cuts at: 0 63 467 480 1913 2251 2320 2496
Size: 63 404 13 1433 338 69 176
Fragments arranged by size:
1433 404 338 176 69 63 13
EarI CTCTTCn'nnn_
Cuts at: 0 1183 2496
Size: 1183 1313
EcoICRI GAG'CTC
Cuts at: 0 1943 2496
Size: 1943 553
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 364 654 1400 2495 2496
Size: 265 99 290 746 1095 1
Fragments arranged by size:
1095 746 290 265 99 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 252 2496
Size: 252 2244
EcoO109I rG'GnC_Cy
Cuts at: 0 1079 2098 2496
Size: 1079 1019 398
Fragments arranged by size:
1079 1019 398
EcoRI G'AATT_C
Cuts at: 0 663 2496
Size: 663 1833
EcoRV GAT'ATC
Cuts at: 0 693 1323 2496
Size: 693 630 1173
Fragments arranged by size:
1173 693 630
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 450 2496
Size: 450 2046
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 667 699 2496
Size: 667 32 1797
Fragments arranged by size:
1797 667 32
HincII GTy'rAC
Cuts at: 0 822 1506 2496
Size: 822 684 990
Fragments arranged by size:
990 822 684
HindIII A'AGCT_T
Cuts at: 0 2130 2496
Size: 2130 366
MfeI C'AATT_G
Cuts at: 0 854 2496
Size: 854 1642
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1604 2496
Size: 1604 892
MscI TGG'CCA
Cuts at: 0 65 482 1915 2496
Size: 65 417 1433 581
Fragments arranged by size:
1433 581 417 65
MslI CAynn'nnrTG
Cuts at: 0 208 343 685 781 1014 1750 2496
Size: 208 135 342 96 233 736 746
Fragments arranged by size:
746 736 342 233 208 135 96
MspA1I CmG'CkG
Cuts at: 0 969 1573 2496
Size: 969 604 923
Fragments arranged by size:
969 923 604
NcoI C'CATG_G
Cuts at: 0 1166 2496
Size: 1166 1330
NdeI CA'TA_TG
Cuts at: 0 915 2496
Size: 915 1581
NheI G'CTAG_C
Cuts at: 0 1998 2496
Size: 1998 498
NsiI A_TGCA'T
Cuts at: 0 915 2496
Size: 915 1581
NspI r_CATG'y
Cuts at: 0 1019 2496
Size: 1019 1477
PciI A'CATG_T
Cuts at: 0 1015 2496
Size: 1015 1481
PflMI CCAn_nnn'nTGG
Cuts at: 0 1444 2496
Size: 1444 1052
PfoI T'CCnGG_A
Cuts at: 0 1046 2496
Size: 1046 1450
PmlI CAC'GTG
Cuts at: 0 1842 2496
Size: 1842 654
PpuMI rG'GwC_Cy
Cuts at: 0 1079 2098 2496
Size: 1079 1019 398
Fragments arranged by size:
1079 1019 398
PstI C_TGCA'G
Cuts at: 0 2296 2496
Size: 2296 200
PvuII CAG'CTG
Cuts at: 0 1573 2496
Size: 1573 923
SacI G_AGCT'C
Cuts at: 0 1945 2496
Size: 1945 551
SanDI GG'GwC_CC
Cuts at: 0 1079 2098 2496
Size: 1079 1019 398
Fragments arranged by size:
1079 1019 398
SfcI C'TryA_G
Cuts at: 0 1134 1739 2292 2496
Size: 1134 605 553 204
Fragments arranged by size:
1134 605 553 204
SmlI C'TyrA_G
Cuts at: 0 1938 2465 2496
Size: 1938 527 31
Fragments arranged by size:
1938 527 31
SspI AAT'ATT
Cuts at: 0 711 811 2496
Size: 711 100 1685
Fragments arranged by size:
1685 711 100
StyI C'CwwG_G
Cuts at: 0 248 890 1166 1769 2003 2258 2496
Size: 248 642 276 603 234 255 238
Fragments arranged by size:
642 603 276 255 248 238 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 911 2496
Size: 911 1585
TatI w'GTAC_w
Cuts at: 0 533 1250 1631 2496
Size: 533 717 381 865
Fragments arranged by size:
865 717 533 381
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 66 98 299 331 2496
Size: 66 32 201 32 2165
Fragments arranged by size:
2165 201 66 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1164 1195 2355 2496
Size: 1164 31 1160 141
Fragments arranged by size:
1164 1160 141 31
XmnI GAAnn'nnTTC
Cuts at: 0 667 2496
Size: 667 1829
Enzymes that do cut:
AccI AclI AcuI AflIII AleI AlwNI ApaLI ApoI
AseI AvaI BamHI BanII BbsI BcgI BciVI BclI
BfrBI BglII Bme1580I BmrI BmtI BpmI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI
Bsp1286I BspMI BsrDI BstEII BstXI BstYI BstZ17I BtgI
BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI Hin4I HincII HindIII MfeI MmeI
MscI MslI MspA1I NcoI NdeI NheI NsiI NspI
PciI PflMI PfoI PmlI PpuMI PstI PvuII SacI
SanDI SfcI SmlI SspI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AlfI
AloI ApaI AscI AsiSI AvrII BaeI BanI BbeI
BbvCI BglI BlpI BmgBI BplI BsaI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI Bsu36I BtgZI ClaI DraI
DraIII DrdI EagI EciI FseI FspI FspAI HaeII
HpaI KasI KpnI MluI NaeI NarI NgoMIV NotI
NruI PacI PasI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI
XmaI ZraI