Restriction map for
pFN21AE5728
(Linear) (Six-base) MAPSORT of: KIEE5728.seq Check: 145 from: 1 to: 2778
KIEE5728.orf
With 182 enzymes: SgfI *
December 5, 2014 13:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 828 832 1542 1603 1977 2778
Size: 757 71 4 710 61 374 801
Fragments arranged by size:
801 757 710 374 71 61 4
AfeI AGC'GCT
Cuts at: 0 1990 2778
Size: 1990 788
AflIII A'CryG_T
Cuts at: 0 225 2778
Size: 225 2553
AhdI GACnn_n'nnGTC
Cuts at: 0 1401 2778
Size: 1401 1377
AleI CACnn'nnGTG
Cuts at: 0 1849 2778
Size: 1849 929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 423 2778
Size: 423 2355
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 550 582 1885 1917 2778
Size: 550 32 1303 32 861
Fragments arranged by size:
1303 861 550 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 124 1407 2275 2778
Size: 124 1283 868 503
Fragments arranged by size:
1283 868 503 124
ApaI G_GGCC'C
Cuts at: 0 2137 2186 2499 2772 2778
Size: 2137 49 313 273 6
Fragments arranged by size:
2137 313 273 49 6
ApaLI G'TGCA_C
Cuts at: 0 205 576 1615 2778
Size: 205 371 1039 1163
Fragments arranged by size:
1163 1039 371 205
AscI GG'CGCG_CC
Cuts at: 0 2697 2778
Size: 2697 81
AvaI C'yCGr_G
Cuts at: 0 277 344 2179 2232 2491 2646 2778
Size: 277 67 1835 53 259 155 132
Fragments arranged by size:
1835 277 259 155 132 67 53
BamHI G'GATC_C
Cuts at: 0 1325 1331 2778
Size: 1325 6 1447
Fragments arranged by size:
1447 1325 6
BanI G'GyrC_C
Cuts at: 0 1350 1937 2015 2292 2487 2566 2778
Size: 1350 587 78 277 195 79 212
Fragments arranged by size:
1350 587 277 212 195 79 78
BanII G_rGCy'C
Cuts at: 0 2137 2179 2186 2499 2772 2778
Size: 2137 42 7 313 273 6
Fragments arranged by size:
2137 313 273 42 7 6
BbeI G_GCGC'C
Cuts at: 0 1354 2019 2296 2570 2778
Size: 1354 665 277 274 208
Fragments arranged by size:
1354 665 277 274 208
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 532 566 2665 2699 2778
Size: 532 34 2099 34 79
Fragments arranged by size:
2099 532 79 34 34
BclI T'GATC_A
Cuts at: 0 1089 2778
Size: 1089 1689
BglI GCCn_nnn'nGGC
Cuts at: 0 1258 1607 2068 2365 2642 2778
Size: 1258 349 461 297 277 136
Fragments arranged by size:
1258 461 349 297 277 136
BlpI GC'TnA_GC
Cuts at: 0 366 1858 2778
Size: 366 1492 920
Fragments arranged by size:
1492 920 366
Bme1580I G_kGCm'C
Cuts at: 0 209 284 580 1619 2137 2186 2492 2499
Size: 209 75 296 1039 518 49 306 7
Cuts at: 2499 2772 2778
Size: 273 6
Fragments arranged by size:
1039 518 306 296 273 209 75 49
7 6
BmgBI CAC'GTC
Cuts at: 0 1322 1398 1742 2778
Size: 1322 76 344 1036
Fragments arranged by size:
1322 1036 344 76
BmrI ACTGGGnnnn_n'
Cuts at: 0 168 658 1230 2778
Size: 168 490 572 1548
Fragments arranged by size:
1548 572 490 168
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2142 2778
Size: 2142 636
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 807 2605 2778
Size: 451 356 1798 173
Fragments arranged by size:
1798 451 356 173
Bpu10I CC'TnA_GC
Cuts at: 0 466 2778
Size: 466 2312
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2140 2146 2778
Size: 2140 6 632
Fragments arranged by size:
2140 632 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 131 1124 2698 2778
Size: 131 993 1574 80
Fragments arranged by size:
1574 993 131 80
BsaAI yAC'GTr
Cuts at: 0 396 2319 2387 2778
Size: 396 1923 68 391
Fragments arranged by size:
1923 396 391 68
BsaBI GATnn'nnATC
Cuts at: 0 439 1330 2778
Size: 439 891 1448
Fragments arranged by size:
1448 891 439
BsaHI Gr'CG_yC
Cuts at: 0 1351 1829 2016 2024 2293 2326 2567 2597
Size: 1351 478 187 8 269 33 241 30
Cuts at: 2597 2778
Size: 181
Fragments arranged by size:
1351 478 269 241 187 181 33 30
8
BsaWI w'CCGG_w
Cuts at: 0 1328 2091 2778
Size: 1328 763 687
Fragments arranged by size:
1328 763 687
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 769 781 799 811 1885 1915 2704 2734
Size: 769 12 18 12 1074 30 789 30
Cuts at: 2734 2778
Size: 44
Fragments arranged by size:
1074 789 769 44 30 30 18 12
12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 793 796 1732 1966 2752 2778
Size: 793 3 936 234 786 26
Fragments arranged by size:
936 793 786 234 26 3
BseYI C'CCAG_C
Cuts at: 0 1548 1569 2778
Size: 1548 21 1209
Fragments arranged by size:
1548 1209 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1177 1285 1420 1464 2341 2677 2778
Size: 1177 108 135 44 877 336 101
Fragments arranged by size:
1177 877 336 135 108 101 44
BsiEI CG_ry'CG
Cuts at: 0 672 1670 2039 2080 2173 2360 2377 2468
Size: 672 998 369 41 93 187 17 91
Cuts at: 2468 2531 2612 2637 2778
Size: 63 81 25 141
Fragments arranged by size:
998 672 369 187 141 93 91 81
63 41 25 17
BsiHKAI G_wGCw'C
Cuts at: 0 209 580 1178 1619 2156 2537 2714 2778
Size: 209 371 598 441 537 381 177 64
Fragments arranged by size:
598 537 441 381 371 209 177 64
BsmI GAATG_Cn'
Cuts at: 0 412 2778
Size: 412 2366
BsmBI CGTCTCn'nnnn_
Cuts at: 0 381 2089 2778
Size: 381 1708 689
Fragments arranged by size:
1708 689 381
Bsp1286I G_dGCh'C
Cuts at: 0 209 284 580 1178 1619 2137 2156 2179
Size: 209 75 296 598 441 518 19 23
Cuts at: 2179 2186 2492 2499 2537 2714 2772 2778
Size: 7 306 7 38 177 58 6
Fragments arranged by size:
598 518 441 306 296 209 177 75
58 38 23 19 7 7 6
BspEI T'CCGG_A
Cuts at: 0 1328 2778
Size: 1328 1450
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 149 538 1040 2778
Size: 149 389 502 1738
Fragments arranged by size:
1738 502 389 149
BsrBI CCG'CTC
Cuts at: 0 175 504 792 1674 2169 2405 2778
Size: 175 329 288 882 495 236 373
Fragments arranged by size:
882 495 373 329 288 236 175
BsrDI GCAATG_nn'
Cuts at: 0 320 2778
Size: 320 2458
BsrFI r'CCGG_y
Cuts at: 0 1797 1912 1934 2109 2355 2652 2778
Size: 1797 115 22 175 246 297 126
Fragments arranged by size:
1797 297 246 175 126 115 22
BsrGI T'GTAC_A
Cuts at: 0 473 2778
Size: 473 2305
BssHII G'CGCG_C
Cuts at: 0 1524 1875 1979 2121 2243 2299 2333 2351
Size: 1524 351 104 142 122 56 34 18
Cuts at: 2351 2434 2436 2479 2481 2614 2697 2761 2778
Size: 83 2 43 2 133 83 64 17
Fragments arranged by size:
1524 351 142 133 122 104 83 83
64 56 43 34 18 17 2 2
BssSI C'ACGA_G
Cuts at: 0 579 1948 2778
Size: 579 1369 830
Fragments arranged by size:
1369 830 579
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1182 2151 2197 2778
Size: 1182 969 46 581
Fragments arranged by size:
1182 969 581 46
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1548 2148 2778
Size: 1548 600 630
Fragments arranged by size:
1548 630 600
BstYI r'GATC_y
Cuts at: 0 1025 1325 1331 2778
Size: 1025 300 6 1447
Fragments arranged by size:
1447 1025 300 6
BtgI C'CryG_G
Cuts at: 0 110 1118 1376 1535 1819 2066 2186 2360
Size: 110 1008 258 159 284 247 120 174
Cuts at: 2360 2637 2778
Size: 277 141
Fragments arranged by size:
1008 284 277 258 247 174 159 141
120 110
BtsI GCAGTG_nn'
Cuts at: 0 1062 1208 2778
Size: 1062 146 1570
Fragments arranged by size:
1570 1062 146
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 512 547 2778
Size: 512 35 2231
Fragments arranged by size:
2231 512 35
DraIII CAC_nnn'GTG
Cuts at: 0 288 575 2778
Size: 288 287 2203
Fragments arranged by size:
2203 288 287
DrdI GACnn_nn'nnGTC
Cuts at: 0 1764 2778
Size: 1764 1014
EaeI y'GGCC_r
Cuts at: 0 131 605 1409 1538 1667 1733 2036 2069
Size: 131 474 804 129 129 66 303 33
Cuts at: 2069 2077 2170 2277 2357 2374 2528 2634 2778
Size: 8 93 107 80 17 154 106 144
Fragments arranged by size:
804 474 303 154 144 131 129 129
107 106 93 80 66 33 17 8
EagI C'GGCC_G
Cuts at: 0 1667 2036 2077 2170 2357 2374 2528 2634
Size: 1667 369 41 93 187 17 154 106
Cuts at: 2634 2778
Size: 144
Fragments arranged by size:
1667 369 187 154 144 106 93 41
17
EarI CTCTTCn'nnn_
Cuts at: 0 905 2778
Size: 905 1873
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 187 1632 1816 2778
Size: 187 1445 184 962
Fragments arranged by size:
1445 962 187 184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 757 807 828 832 1542 1603 1977
Size: 451 306 50 21 4 710 61 374
Cuts at: 1977 2605 2778
Size: 628 173
Fragments arranged by size:
710 628 451 374 306 173 61 50
21 4
EcoO109I rG'GnC_Cy
Cuts at: 0 162 247 675 888 1044 2495 2496 2768
Size: 162 85 428 213 156 1451 1 272
Cuts at: 2768 2778
Size: 10
Fragments arranged by size:
1451 428 272 213 162 156 85 10
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1923 2778
Size: 1923 855
FspI TGC'GCA
Cuts at: 0 2452 2778
Size: 2452 326
HaeII r_GCGC'y
Cuts at: 0 1354 1992 2000 2019 2261 2296 2371 2570
Size: 1354 638 8 19 242 35 75 199
Cuts at: 2570 2667 2778
Size: 97 111
Fragments arranged by size:
1354 638 242 199 111 97 75 35
19 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 159 191 1387 1419 2524 2556 2778
Size: 159 32 1196 32 1105 32 222
Fragments arranged by size:
1196 1105 222 159 32 32 32
HincII GTy'rAC
Cuts at: 0 2460 2778
Size: 2460 318
KasI G'GCGC_C
Cuts at: 0 1350 2015 2292 2566 2778
Size: 1350 665 277 274 212
Fragments arranged by size:
1350 665 277 274 212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1730 2778
Size: 1730 1048
MscI TGG'CCA
Cuts at: 0 133 607 1540 2778
Size: 133 474 933 1238
Fragments arranged by size:
1238 933 474 133
MslI CAynn'nnrTG
Cuts at: 0 1315 1849 2317 2778
Size: 1315 534 468 461
Fragments arranged by size:
1315 534 468 461
MspA1I CmG'CkG
Cuts at: 0 1786 1821 2013 2044 2148 2188 2264 2661
Size: 1786 35 192 31 104 40 76 397
Cuts at: 2661 2778
Size: 117
Fragments arranged by size:
1786 397 192 117 104 76 40 35
31
NaeI GCC'GGC
Cuts at: 0 1799 1914 2357 2778
Size: 1799 115 443 421
Fragments arranged by size:
1799 443 421 115
NarI GG'CG_CC
Cuts at: 0 1351 2016 2293 2567 2778
Size: 1351 665 277 274 211
Fragments arranged by size:
1351 665 277 274 211
NcoI C'CATG_G
Cuts at: 0 1118 2778
Size: 1118 1660
NgoMIV G'CCGG_C
Cuts at: 0 1797 1912 2355 2778
Size: 1797 115 443 423
Fragments arranged by size:
1797 443 423 115
NotI GC'GGCC_GC
Cuts at: 0 2036 2077 2778
Size: 2036 41 701
Fragments arranged by size:
2036 701 41
NspI r_CATG'y
Cuts at: 0 229 640 2778
Size: 229 411 2138
Fragments arranged by size:
2138 411 229
PciI A'CATG_T
Cuts at: 0 225 2778
Size: 225 2553
PflMI CCAn_nnn'nTGG
Cuts at: 0 2275 2778
Size: 2275 503
PfoI T'CCnGG_A
Cuts at: 0 2114 2778
Size: 2114 664
PmlI CAC'GTG
Cuts at: 0 396 2319 2778
Size: 396 1923 459
Fragments arranged by size:
1923 459 396
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1648 1680 1885 1917 2778
Size: 1648 32 205 32 861
Fragments arranged by size:
1648 861 205 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 162 247 675 888 1044 2778
Size: 162 85 428 213 156 1734
Fragments arranged by size:
1734 428 213 162 156 85
PspOMI G'GGCC_C
Cuts at: 0 2133 2182 2495 2768 2778
Size: 2133 49 313 273 10
Fragments arranged by size:
2133 313 273 49 10
PstI C_TGCA'G
Cuts at: 0 308 2778
Size: 308 2470
PvuII CAG'CTG
Cuts at: 0 2148 2778
Size: 2148 630
RsrII CG'GwC_CG
Cuts at: 0 1428 2778
Size: 1428 1350
SacII CC_GC'GG
Cuts at: 0 1822 2189 2778
Size: 1822 367 589
Fragments arranged by size:
1822 589 367
SanDI GG'GwC_CC
Cuts at: 0 247 2778
Size: 247 2531
SexAI A'CCwGG_T
Cuts at: 0 123 677 2778
Size: 123 554 2101
Fragments arranged by size:
2101 554 123
SfcI C'TryA_G
Cuts at: 0 304 2424 2778
Size: 304 2120 354
Fragments arranged by size:
2120 354 304
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1607 2642 2778
Size: 1607 1035 136
Fragments arranged by size:
1607 1035 136
SfoI GGC'GCC
Cuts at: 0 1352 2017 2294 2568 2778
Size: 1352 665 277 274 210
Fragments arranged by size:
1352 665 277 274 210
SgrAI Cr'CCGG_yG
Cuts at: 0 1797 2778
Size: 1797 981
SmaI CCC'GGG
Cuts at: 0 279 346 2181 2234 2493 2648 2778
Size: 279 67 1835 53 259 155 130
Fragments arranged by size:
1835 279 259 155 130 67 53
SmlI C'TyrA_G
Cuts at: 0 2155 2161 2778
Size: 2155 6 617
Fragments arranged by size:
2155 617 6
SrfI GCCC'GGGC
Cuts at: 0 279 2778
Size: 279 2499
StyI C'CwwG_G
Cuts at: 0 1037 1118 2778
Size: 1037 81 1660
Fragments arranged by size:
1660 1037 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 95 1023 2154 2722 2778
Size: 95 928 1131 568 56
Fragments arranged by size:
1131 928 568 95 56
TatI w'GTAC_w
Cuts at: 0 473 1838 2778
Size: 473 1365 940
Fragments arranged by size:
1365 940 473
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 769 781 801 813 1210 1242 2332 2364
Size: 769 12 20 12 397 32 1090 32
Cuts at: 2364 2704 2736 2778
Size: 340 32 42
Fragments arranged by size:
1090 769 397 340 42 32 32 32
20 12 12
Tth111I GACn'n_nGTC
Cuts at: 0 85 2778
Size: 85 2693
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 141 378 960 2778
Size: 141 237 582 1818
Fragments arranged by size:
1818 582 237 141
XmaI C'CCGG_G
Cuts at: 0 277 344 2179 2232 2491 2646 2778
Size: 277 67 1835 53 259 155 132
Fragments arranged by size:
1835 277 259 155 132 67 53
Enzymes that do cut:
AcuI AfeI AflIII AhdI AleI AlfI AloI AlwNI
ApaI ApaLI AscI AvaI BamHI BanI BanII BbeI
BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstXI BstYI BtgI BtsI CspCI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I FalI
FspI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI
PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII
RsrII SacII SanDI SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SrfI StyI TaqII TatI TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI ApoI
AseI AsiSI AvrII BaeI BbsI BbvCI BciVI BfrBI
BglII BmtI BsiWI BspHI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI DraI EcoICRI EcoNI EcoRI EcoRV FseI
FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI
NruI NsiI PacI PasI PmeI PshAI PsiI PspXI
PsrI PvuI SacI SalI SapI SbfI ScaI SgfI
SnaBI SpeI SphI SspI StuI SwaI XbaI XhoI
XmnI ZraI