Restriction map for
pFN21AE5722
(Linear) (Six-base) MAPSORT of: KIEE5722.seq Check: 626 from: 1 to: 2610
KIEE5722.orf
With 182 enzymes: SgfI *
December 5, 2014 13:08 ..
AccI GT'mk_AC
Cuts at: 0 2316 2610
Size: 2316 294
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 529 597 1526 2610
Size: 220 309 68 929 1084
Fragments arranged by size:
1084 929 309 220 68
AhdI GACnn_n'nnGTC
Cuts at: 0 433 2610
Size: 433 2177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1466 2610
Size: 1466 1144
AlwNI CAG_nnn'CTG
Cuts at: 0 1453 2610
Size: 1453 1157
ApaI G_GGCC'C
Cuts at: 0 2063 2610
Size: 2063 547
ApaLI G'TGCA_C
Cuts at: 0 20 2610
Size: 20 2590
ApoI r'AATT_y
Cuts at: 0 283 312 1921 2098 2110 2485 2610
Size: 283 29 1609 177 12 375 125
Fragments arranged by size:
1609 375 283 177 125 29 12
AvrII C'CTAG_G
Cuts at: 0 1149 2610
Size: 1149 1461
BamHI G'GATC_C
Cuts at: 0 1585 2610
Size: 1585 1025
BanII G_rGCy'C
Cuts at: 0 1382 1576 2063 2610
Size: 1382 194 487 547
Fragments arranged by size:
1382 547 487 194
BbsI GAAGACnn'nnnn_
Cuts at: 0 555 1837 1994 2610
Size: 555 1282 157 616
Fragments arranged by size:
1282 616 555 157
BclI T'GATC_A
Cuts at: 0 75 2610
Size: 75 2535
Bme1580I G_kGCm'C
Cuts at: 0 24 1824 2063 2610
Size: 24 1800 239 547
Fragments arranged by size:
1800 547 239 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1717 2610
Size: 1717 893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 671 953 1366 1777 1877 2610
Size: 322 349 282 413 411 100 733
Fragments arranged by size:
733 413 411 349 322 282 100
BsaI GGTCTCn'nnnn_
Cuts at: 0 559 1385 2402 2610
Size: 559 826 1017 208
Fragments arranged by size:
1017 826 559 208
BsaWI w'CCGG_w
Cuts at: 0 26 1029 1582 2610
Size: 26 1003 553 1028
Fragments arranged by size:
1028 1003 553 26
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1011 1041 1264 1294 2610
Size: 1011 30 223 30 1316
Fragments arranged by size:
1316 1011 223 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 185 704 1111 1403 1786 2610
Size: 185 519 407 292 383 824
Fragments arranged by size:
824 519 407 383 292 185
BseYI C'CCAG_C
Cuts at: 0 337 1789 2360 2610
Size: 337 1452 571 250
Fragments arranged by size:
1452 571 337 250
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 1255 1439 2296 2610
Size: 680 575 184 857 314
Fragments arranged by size:
857 680 575 314 184
BsiEI CG_ry'CG
Cuts at: 0 2044 2610
Size: 2044 566
BsiHKAI G_wGCw'C
Cuts at: 0 24 1382 1576 2225 2610
Size: 24 1358 194 649 385
Fragments arranged by size:
1358 649 385 194 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1242 2610
Size: 1242 1368
Bsp1286I G_dGCh'C
Cuts at: 0 24 1382 1576 1824 2063 2225 2610
Size: 24 1358 194 248 239 162 385
Fragments arranged by size:
1358 385 248 239 194 162 24
BspEI T'CCGG_A
Cuts at: 0 26 1029 1582 2610
Size: 26 1003 553 1028
Fragments arranged by size:
1028 1003 553 26
BsrDI GCAATG_nn'
Cuts at: 0 2372 2610
Size: 2372 238
BsrFI r'CCGG_y
Cuts at: 0 2043 2239 2610
Size: 2043 196 371
Fragments arranged by size:
2043 371 196
BssSI C'ACGA_G
Cuts at: 0 121 1438 2610
Size: 121 1317 1172
Fragments arranged by size:
1317 1172 121
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1453 2610
Size: 1453 1157
BstBI TT'CG_AA
Cuts at: 0 1870 2610
Size: 1870 740
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1290 2400 2610
Size: 1290 1110 210
Fragments arranged by size:
1290 1110 210
BstYI r'GATC_y
Cuts at: 0 1397 1585 2610
Size: 1397 188 1025
Fragments arranged by size:
1397 1025 188
BstZ17I GTA'TAC
Cuts at: 0 2317 2610
Size: 2317 293
BtgI C'CryG_G
Cuts at: 0 165 1425 2610
Size: 165 1260 1185
Fragments arranged by size:
1260 1185 165
ClaI AT'CG_AT
Cuts at: 0 48 2610
Size: 48 2562
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1638 1673 2610
Size: 1638 35 937
Fragments arranged by size:
1638 937 35
DraI TTT'AAA
Cuts at: 0 821 1686 2610
Size: 821 865 924
Fragments arranged by size:
924 865 821
EaeI y'GGCC_r
Cuts at: 0 2041 2241 2610
Size: 2041 200 369
Fragments arranged by size:
2041 369 200
EagI C'GGCC_G
Cuts at: 0 2041 2610
Size: 2041 569
EarI CTCTTCn'nnn_
Cuts at: 0 770 2558 2610
Size: 770 1788 52
Fragments arranged by size:
1788 770 52
EcoICRI GAG'CTC
Cuts at: 0 1380 1574 2610
Size: 1380 194 1036
Fragments arranged by size:
1380 1036 194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 322 529 597 671 953 1366 1526
Size: 220 102 207 68 74 282 413 160
Cuts at: 1526 1777 1877 2610
Size: 251 100 733
Fragments arranged by size:
733 413 282 251 220 207 160 102
100 74 68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1976 2610
Size: 1976 634
EcoO109I rG'GnC_Cy
Cuts at: 0 595 2059 2610
Size: 595 1464 551
Fragments arranged by size:
1464 595 551
EcoRI G'AATT_C
Cuts at: 0 312 2098 2610
Size: 312 1786 512
Fragments arranged by size:
1786 512 312
EcoRV GAT'ATC
Cuts at: 0 52 2093 2214 2610
Size: 52 2041 121 396
Fragments arranged by size:
2041 396 121 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 336 368 419 451 1164 1196 1382 1414
Size: 336 32 51 32 713 32 186 32
Cuts at: 1414 2077 2109 2610
Size: 663 32 501
Fragments arranged by size:
713 663 501 336 186 51 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 228 1008 2610
Size: 228 780 1602
Fragments arranged by size:
1602 780 228
HindIII A'AGCT_T
Cuts at: 0 1113 2551 2610
Size: 1113 1438 59
Fragments arranged by size:
1438 1113 59
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1251 2610
Size: 1251 1359
MslI CAynn'nnrTG
Cuts at: 0 170 847 2610
Size: 170 677 1763
Fragments arranged by size:
1763 677 170
MspA1I CmG'CkG
Cuts at: 0 1450 2610
Size: 1450 1160
NaeI GCC'GGC
Cuts at: 0 2241 2610
Size: 2241 369
NcoI C'CATG_G
Cuts at: 0 165 2610
Size: 165 2445
NgoMIV G'CCGG_C
Cuts at: 0 2239 2610
Size: 2239 371
NspI r_CATG'y
Cuts at: 0 14 130 1002 2610
Size: 14 116 872 1608
Fragments arranged by size:
1608 872 116 14
PfoI T'CCnGG_A
Cuts at: 0 648 969 2610
Size: 648 321 1641
Fragments arranged by size:
1641 648 321
PspOMI G'GGCC_C
Cuts at: 0 2059 2610
Size: 2059 551
PstI C_TGCA'G
Cuts at: 0 414 2610
Size: 414 2196
PvuII CAG'CTG
Cuts at: 0 1450 2610
Size: 1450 1160
SacI G_AGCT'C
Cuts at: 0 1382 1576 2610
Size: 1382 194 1034
Fragments arranged by size:
1382 1034 194
SapI GCTCTTCn'nnn_
Cuts at: 0 2558 2610
Size: 2558 52
SfcI C'TryA_G
Cuts at: 0 410 750 2610
Size: 410 340 1860
Fragments arranged by size:
1860 410 340
SspI AAT'ATT
Cuts at: 0 791 2497 2610
Size: 791 1706 113
Fragments arranged by size:
1706 791 113
StuI AGG'CCT
Cuts at: 0 1026 1154 2610
Size: 1026 128 1456
Fragments arranged by size:
1456 1026 128
StyI C'CwwG_G
Cuts at: 0 165 1149 2514 2610
Size: 165 984 1365 96
Fragments arranged by size:
1365 984 165 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 975 2610
Size: 975 1635
TatI w'GTAC_w
Cuts at: 0 2246 2610
Size: 2246 364
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1011 1043 1264 1296 1463 1495 1907 1939
Size: 1011 32 221 32 167 32 412 32
Cuts at: 1939 2258 2290 2610
Size: 319 32 320
Fragments arranged by size:
1011 412 320 319 221 167 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 646 916 2199 2610
Size: 646 270 1283 411
Fragments arranged by size:
1283 646 411 270
Enzymes that do cut:
AccI AcuI AhdI AlfI AlwNI ApaI ApaLI ApoI
AvrII BamHI BanII BbsI BclI Bme1580I BplI BpmI
BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I BtgI ClaI CspCI DraI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
Hin4I HincII HindIII MmeI MslI MspA1I NaeI NcoI
NgoMIV NspI PfoI PspOMI PstI PvuII SacI SapI
SfcI SspI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI AscI AseI AsiSI AvaI BaeI BanI
BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsiWI BsmI BspHI BspMI BsrBI BsrGI BssHII BstEII
Bsu36I BtgZI BtsI DraIII DrdI EciI FalI FseI
FspI FspAI HaeII HpaI KasI KpnI MfeI MluI
MscI NarI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI
XhoI XmaI XmnI ZraI