Restriction map for
pFN21AE5721
(Linear) (Six-base) MAPSORT of: KIEE5721.seq Check: 2860 from: 1 to: 2070
KIEE5721.orf
With 182 enzymes: SgfI *
December 5, 2014 13:08 ..
AccI GT'mk_AC
Cuts at: 0 998 1049 1066 1496 1821 1901 2070
Size: 998 51 17 430 325 80 169
Fragments arranged by size:
998 430 325 169 80 51 17
AclI AA'CG_TT
Cuts at: 0 1403 2070
Size: 1403 667
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 876 2070
Size: 176 700 1194
Fragments arranged by size:
1194 700 176
AlwNI CAG_nnn'CTG
Cuts at: 0 246 1962 2070
Size: 246 1716 108
Fragments arranged by size:
1716 246 108
ApaI G_GGCC'C
Cuts at: 0 2057 2070
Size: 2057 13
ApoI r'AATT_y
Cuts at: 0 1021 1225 1990 2017 2070
Size: 1021 204 765 27 53
Fragments arranged by size:
1021 765 204 53 27
AseI AT'TA_AT
Cuts at: 0 1010 2070
Size: 1010 1060
AvaI C'yCGr_G
Cuts at: 0 480 2070
Size: 480 1590
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 980 1013 2070
Size: 980 33 1057
Fragments arranged by size:
1057 980 33
BanI G'GyrC_C
Cuts at: 0 253 2070
Size: 253 1817
BanII G_rGCy'C
Cuts at: 0 2057 2070
Size: 2057 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1611 1645 2070
Size: 1611 34 425
Fragments arranged by size:
1611 425 34
BfrBI ATG'CAT
Cuts at: 0 943 1263 2070
Size: 943 320 807
Fragments arranged by size:
943 807 320
BglII A'GATC_T
Cuts at: 0 1863 2070
Size: 1863 207
Bme1580I G_kGCm'C
Cuts at: 0 2057 2070
Size: 2057 13
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1526 2060 2070
Size: 1526 534 10
Fragments arranged by size:
1526 534 10
BsaAI yAC'GTr
Cuts at: 0 615 1047 2070
Size: 615 432 1023
Fragments arranged by size:
1023 615 432
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1086 1116 2070
Size: 1086 30 954
Fragments arranged by size:
1086 954 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 436 463 2070
Size: 436 27 1607
Fragments arranged by size:
1607 436 27
BsmI GAATG_Cn'
Cuts at: 0 945 1215 1643 2070
Size: 945 270 428 427
Fragments arranged by size:
945 428 427 270
Bsp1286I G_dGCh'C
Cuts at: 0 2057 2070
Size: 2057 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 254 2070
Size: 254 1816
BsrBI CCG'CTC
Cuts at: 0 2044 2070
Size: 2044 26
BsrDI GCAATG_nn'
Cuts at: 0 257 1835 2070
Size: 257 1578 235
Fragments arranged by size:
1578 257 235
BsrGI T'GTAC_A
Cuts at: 0 530 1547 2070
Size: 530 1017 523
Fragments arranged by size:
1017 530 523
BstAPI GCAn_nnn'nTGC
Cuts at: 0 246 1260 1393 2070
Size: 246 1014 133 677
Fragments arranged by size:
1014 677 246 133
BstBI TT'CG_AA
Cuts at: 0 1269 2070
Size: 1269 801
BstEII G'GTnAC_C
Cuts at: 0 664 2070
Size: 664 1406
BstYI r'GATC_y
Cuts at: 0 1827 1863 2070
Size: 1827 36 207
Fragments arranged by size:
1827 207 36
BstZ17I GTA'TAC
Cuts at: 0 1050 2070
Size: 1050 1020
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1631 2070
Size: 1631 439
BtsI GCAGTG_nn'
Cuts at: 0 1857 2070
Size: 1857 213
ClaI AT'CG_AT
Cuts at: 0 638 2070
Size: 638 1432
DraI TTT'AAA
Cuts at: 0 738 2070
Size: 738 1332
EaeI y'GGCC_r
Cuts at: 0 1026 1685 2070
Size: 1026 659 385
Fragments arranged by size:
1026 659 385
EarI CTCTTCn'nnn_
Cuts at: 0 331 440 1781 1865 2070
Size: 331 109 1341 84 205
Fragments arranged by size:
1341 331 205 109 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 876 1526 2060 2070
Size: 176 700 650 534 10
Fragments arranged by size:
700 650 534 176 10
EcoO109I rG'GnC_Cy
Cuts at: 0 2035 2070
Size: 2035 35
EcoRV GAT'ATC
Cuts at: 0 1533 2070
Size: 1533 537
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 601 633 736 768 864 896
Size: 130 32 439 32 103 32 96 32
Cuts at: 896 1930 1962 2070
Size: 1034 32 108
Fragments arranged by size:
1034 439 130 108 103 96 32 32
32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 926 1226 2070
Size: 749 177 300 844
Fragments arranged by size:
844 749 300 177
MslI CAynn'nnrTG
Cuts at: 0 938 2070
Size: 938 1132
NsiI A_TGCA'T
Cuts at: 0 945 1265 2070
Size: 945 320 805
Fragments arranged by size:
945 805 320
NspI r_CATG'y
Cuts at: 0 116 563 947 2070
Size: 116 447 384 1123
Fragments arranged by size:
1123 447 384 116
PfoI T'CCnGG_A
Cuts at: 0 1503 2070
Size: 1503 567
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 689 721 2070
Size: 689 32 1349
Fragments arranged by size:
1349 689 32
PpuMI rG'GwC_Cy
Cuts at: 0 2035 2070
Size: 2035 35
PsiI TTA'TAA
Cuts at: 0 203 2070
Size: 203 1867
PspOMI G'GGCC_C
Cuts at: 0 2053 2070
Size: 2053 17
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1173 1205 2070
Size: 1173 32 865
Fragments arranged by size:
1173 865 32
SapI GCTCTTCn'nnn_
Cuts at: 0 331 2070
Size: 331 1739
SexAI A'CCwGG_T
Cuts at: 0 1915 2070
Size: 1915 155
SfcI C'TryA_G
Cuts at: 0 1497 2070
Size: 1497 573
SnaBI TAC'GTA
Cuts at: 0 615 1047 2070
Size: 615 432 1023
Fragments arranged by size:
1023 615 432
SphI G_CATG'C
Cuts at: 0 116 2070
Size: 116 1954
SspI AAT'ATT
Cuts at: 0 24 595 1692 2070
Size: 24 571 1097 378
Fragments arranged by size:
1097 571 378 24
StyI C'CwwG_G
Cuts at: 0 1979 2070
Size: 1979 91
TatI w'GTAC_w
Cuts at: 0 530 1547 2070
Size: 530 1017 523
Fragments arranged by size:
1017 530 523
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 930 962 2070
Size: 930 32 1108
Fragments arranged by size:
1108 930 32
Tth111I GACn'n_nGTC
Cuts at: 0 625 2070
Size: 625 1445
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1779 2070
Size: 1779 291
Enzymes that do cut:
AccI AclI AcuI AlwNI ApaI ApoI AseI AvaI
BaeI BanI BanII BcgI BfrBI BglII Bme1580I BpmI
BsaAI BsaXI BseRI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrGI BstAPI BstBI BstEII BstYI BstZ17I BtgZI BtsI
ClaI DraI EaeI EarI Eco57MI EcoO109I EcoRV Hin4I
MmeI MslI NsiI NspI PfoI PpiI PpuMI PsiI
PspOMI PsrI SapI SexAI SfcI SnaBI SphI SspI
StyI TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AsiSI AvrII BamHI
BbeI BbsI BbvCI BciVI BclI BglI BlpI BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspHI BsrFI BssHII BssSI BstXI Bsu36I BtgI CspCI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MscI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PmeI PmlI PshAI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SpeI SrfI StuI
SwaI TaqII XbaI XhoI XmaI XmnI ZraI