Restriction map for
pFN21AE5720
(Linear) (Six-base) MAPSORT of: KIEE5720.seq Check: 3301 from: 1 to: 1848
KIEE5720.orf
With 182 enzymes: SgfI *
December 5, 2014 13:08 ..
AccI GT'mk_AC
Cuts at: 0 461 1848
Size: 461 1387
AclI AA'CG_TT
Cuts at: 0 1424 1848
Size: 1424 424
AfeI AGC'GCT
Cuts at: 0 278 1848
Size: 278 1570
AflII C'TTAA_G
Cuts at: 0 1266 1519 1848
Size: 1266 253 329
Fragments arranged by size:
1266 329 253
ApaLI G'TGCA_C
Cuts at: 0 45 1848
Size: 45 1803
ApoI r'AATT_y
Cuts at: 0 75 714 1848
Size: 75 639 1134
Fragments arranged by size:
1134 639 75
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 340 373 1254 1287 1848
Size: 340 33 881 33 561
Fragments arranged by size:
881 561 340 33 33
BamHI G'GATC_C
Cuts at: 0 1122 1848
Size: 1122 726
BanII G_rGCy'C
Cuts at: 0 236 1848
Size: 236 1612
BbsI GAAGACnn'nnnn_
Cuts at: 0 11 1848
Size: 11 1837
BciVI GTATCCnnnnn_n'
Cuts at: 0 368 1848
Size: 368 1480
BclI T'GATC_A
Cuts at: 0 1218 1848
Size: 1218 630
BglII A'GATC_T
Cuts at: 0 942 1848
Size: 942 906
Bme1580I G_kGCm'C
Cuts at: 0 49 1848
Size: 49 1799
BmrI ACTGGGnnnn_n'
Cuts at: 0 302 1848
Size: 302 1546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 287 1054 1806 1848
Size: 125 162 767 752 42
Fragments arranged by size:
767 752 162 125 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1027 1057 1848
Size: 1027 30 791
Fragments arranged by size:
1027 791 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1225 1361 1848
Size: 1225 136 487
Fragments arranged by size:
1225 487 136
BseYI C'CCAG_C
Cuts at: 0 1590 1848
Size: 1590 258
BsiHKAI G_wGCw'C
Cuts at: 0 49 1848
Size: 49 1799
Bsp1286I G_dGCh'C
Cuts at: 0 49 236 1848
Size: 49 187 1612
Fragments arranged by size:
1612 187 49
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1315 1776 1848
Size: 1315 461 72
Fragments arranged by size:
1315 461 72
BstXI CCAn_nnnn'nTGG
Cuts at: 0 87 1848
Size: 87 1761
BstYI r'GATC_y
Cuts at: 0 942 1122 1848
Size: 942 180 726
Fragments arranged by size:
942 726 180
BtgI C'CryG_G
Cuts at: 0 1130 1848
Size: 1130 718
BtsI GCAGTG_nn'
Cuts at: 0 252 1084 1848
Size: 252 832 764
Fragments arranged by size:
832 764 252
DraI TTT'AAA
Cuts at: 0 20 1848
Size: 20 1828
DrdI GACnn_nn'nnGTC
Cuts at: 0 207 1848
Size: 207 1641
EaeI y'GGCC_r
Cuts at: 0 759 1848
Size: 759 1089
EarI CTCTTCn'nnn_
Cuts at: 0 218 849 984 1383 1848
Size: 218 631 135 399 465
Fragments arranged by size:
631 465 399 218 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 287 1054 1806 1848
Size: 125 162 767 752 42
Fragments arranged by size:
767 752 162 125 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1785 1848
Size: 1785 63
EcoO109I rG'GnC_Cy
Cuts at: 0 99 1848
Size: 99 1749
EcoRI G'AATT_C
Cuts at: 0 75 1848
Size: 75 1773
EcoRV GAT'ATC
Cuts at: 0 1793 1848
Size: 1793 55
HaeII r_GCGC'y
Cuts at: 0 280 1848
Size: 280 1568
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 727 759 1158 1190 1228 1260 1473 1505
Size: 727 32 399 32 38 32 213 32
Cuts at: 1505 1782 1814 1848
Size: 277 32 34
Fragments arranged by size:
727 399 277 213 38 34 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1356 1848
Size: 1356 492
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 1407 1848
Size: 105 1302 441
Fragments arranged by size:
1302 441 105
MscI TGG'CCA
Cuts at: 0 761 1848
Size: 761 1087
MslI CAynn'nnrTG
Cuts at: 0 1232 1848
Size: 1232 616
MspA1I CmG'CkG
Cuts at: 0 354 1306 1848
Size: 354 952 542
Fragments arranged by size:
952 542 354
NcoI C'CATG_G
Cuts at: 0 1130 1848
Size: 1130 718
NspI r_CATG'y
Cuts at: 0 1779 1848
Size: 1779 69
PasI CC'CwG_GG
Cuts at: 0 601 1848
Size: 601 1247
PflMI CCAn_nnn'nTGG
Cuts at: 0 1830 1848
Size: 1830 18
PpuMI rG'GwC_Cy
Cuts at: 0 99 1848
Size: 99 1749
PvuII CAG'CTG
Cuts at: 0 354 1306 1848
Size: 354 952 542
Fragments arranged by size:
952 542 354
SfcI C'TryA_G
Cuts at: 0 318 492 960 1275 1465 1614 1848
Size: 318 174 468 315 190 149 234
Fragments arranged by size:
468 318 315 234 190 174 149
SmlI C'TyrA_G
Cuts at: 0 1266 1519 1848
Size: 1266 253 329
Fragments arranged by size:
1266 329 253
SspI AAT'ATT
Cuts at: 0 1647 1848
Size: 1647 201
StyI C'CwwG_G
Cuts at: 0 1130 1143 1437 1848
Size: 1130 13 294 411
Fragments arranged by size:
1130 411 294 13
SwaI ATTT'AAAT
Cuts at: 0 20 1848
Size: 20 1828
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 505 948 1848
Size: 505 443 900
Fragments arranged by size:
900 505 443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 510 1848
Size: 510 1338
Enzymes that do cut:
AccI AclI AfeI AflII ApaLI ApoI BaeI BamHI
BanII BbsI BciVI BclI BglII Bme1580I BmrI BpmI
BsaXI BseRI BseYI BsiHKAI Bsp1286I BstAPI BstXI BstYI
BtgI BtsI DraI DrdI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI EcoRV HaeII Hin4I HincII MmeI MscI
MslI MspA1I NcoI NspI PasI PflMI PpuMI PvuII
SfcI SmlI SspI StyI SwaI TaqII XcmI
Enzymes that do not cut:
AarI AatII Acc65I AcuI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI AscI AseI AsiSI AvaI
AvrII BanI BbeI BbvCI BcgI BfrBI BglI BlpI
BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII
EagI EciI EcoICRI FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI